LOCUS       RC209141                8151 bp    DNA     circular  SYN 
DEFINITION  UNC13D (Myc-DDK-tagged)-Human unc-13 homolog D (C. elegans) (UNC13D)
ACCESSION   NM_199242
VERSION     NM_199242.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4308..4399 
                     /label="Myc-DDK"  
     misc_feature    4429..5014 
                     /label="PolyA signal"  
     misc_feature    4486..4505 
                     /label="Restriction Enzyme"  
     misc_feature    5290..5962 
                     /label="ColE1"  
     misc_feature    6392..7186 
                     /label="Kan/Neo"  
     misc_feature    7221..7523 
                     /label="SV40 ori"  
     misc_feature    7609..8086 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4290..4296 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4290 
                     /label="UNC13D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACACTC CTCTCCCATC CGCAGCAGCG CCCTCCCTTC TTGCGCCAGG 
     1081 CCATCAAGAT AAGGCGCCGC AGAGTCAGAG ATCTACAGGA TCCCCCGCCC CAAATGGCCC 
     1141 CGGAGATCCA GCCTCCATCC CACCACTTCT CCCCCGAGCA GCGGGCCCTG CTCTACGAGG 
     1201 ACGCACTCTA CACTGTCTTG CACCGCCTGG GTCATCCTGA GCCCAACCAT GTGACGGAGG 
     1261 CCTCTGAGCT GCTGCGATAC CTGCAGGAGG CCTTCCACGT GGAGCCCGAG GAGCACCAGC 
     1321 AGACACTGCA GCGGGTCAGG GAGCTTGAGA AGCCAATATT TTGTCTGAAG GCAACAGTGA 
     1381 AACAGGCCAA GGGCATTCTG GGCAAAGATG TCAGTGGGTT CAGCGACCCC TACTGCCTGC 
     1441 TGGGCATTGA GCAGGGGGTA GGTGTGCCAG GGGGCAGCCC CGGGTCCCGG CATCGGCAGA 
     1501 AGGCTGTGGT GAGGCACACC ATCCCCGAGG AGGAGACCCA CCGCACGCAG GTCATCACCC 
     1561 AGACACTCAA CCCCGTCTGG GACGAGACCT TCATCCTGGA GTTTGAGGAC ATCACCAATG 
     1621 CGAGCTTTCA TCTGGACATG TGGGACCTGG ACACTGTGGA GTCTGTCCGA CAGAAGCTTG 
     1681 GGGAGCTCAC GGATCTGCAT GGGCTTCGCA GGATCTTTAA AGAGGCCCGG AAGGACAAAG 
     1741 GCCAGGACGA CTTTCTGGGG AACGTGGTTC TGAGGCTGCA GGACCTGCGC TGCCGAGAGG 
     1801 ACCAGTGGTA CCCCCTGGAA CCCCGCACTG AGACCTACCC AGACCGAGGC CAGTGCCACC 
     1861 TCCAGTTCCA ACTCATCCAT AAGCGGAGAG CCACTTCGGC CAGCCGCTCG CAGCCGAGCT 
     1921 ACACCGTGCA CCTCCACCTC CTGCAGCAGC TTGTGTCCCA CGAGGTCACC CAGCACGAGG 
     1981 CGGGAAGCAC CTCCTGGGAC GGGTCGCTGA GTCCCCAGGC TGCCACCGTC CTCTTTCTGC 
     2041 ACGCCACACA GAAGGACCTA TCCGACTTCC ACCAGTCCAT GGCGCAGTGG CTGGCCTACA 
     2101 GCCGCCTCTA CCAGAGCCTG GAGTTCCCCA GCAGCTGCCT CCTGCACCCC ATCACCAGCA 
     2161 TCGAGTACCA GTGGATCCAG GGTCGGCTCA AGGCAGAACA GCAGGAGGAG CTGGCCGCCT 
     2221 CATTCAGCTC CCTGCTGACC TACGGCCTCT CCCTCATCCG GAGGTTCCGC TCTGTCTTCC 
     2281 CCCTCTCTGT CTCGGACTCC CCAGCCCGGC TGCAGTCTCT TCTCAGGGTC CTGGTACAGA 
     2341 TGTGCAAGAT GAAGGCCTTT GGAGAACTGT GCCCCAACAC CGCCCCATTG CCCCAGCTGG 
     2401 TGACTGAGGC CCTGCAGACT GGCACCACTG AATGGTTCCA CCTGAAGCAG CAGCACCATC 
     2461 AACCCATGGT GCAGGGCATC CCGGAGGCAG GCAAGGCCTT GCTGGGCCTG GTACAGGATG 
     2521 TCATTGGCGA CCTGCACCAG TGCCAGCGCA CATGGGACAA GATCTTCCAC AATACCCTCA 
     2581 AGATCCACCT CTTCTCCATG GCTTTCCGGG AGCTGCAGTG GCTGGTGGCC AAGCGGGTGC 
     2641 AGGACCACAC GACGGTTGTG GGTGATGTAG TGTCCCCAGA GATGGGCGAG AGTCTGTTCC 
     2701 AGCTCTACAT CAGCCTCAAG GAGCTCTGCC AGCTGCGCAT GAGCTCCTCA GAGAGGGATG 
     2761 GAGTCCTGGC CCTGGATAAT TTCCACCGCT GGTTCCAGCC GGCCATCCCC TCCTGGCTGC 
     2821 AGAAGACGTA CAACGAGGCC CTGGCGCGGG TGCAGCGCGC TGTGCAGATG GATGAGCTGG 
     2881 TGCCCCTGGG TGAACTGACC AAGCACAGCA CATCAGCGGT GGATCTATCC ACCTGCTTTG 
     2941 CCCAGATCAG CCACACTGCC CGGCAGCTGG ACTGGCCAGA CCCAGAGGAG GCCTTCATGA 
     3001 TTACCGTCAA GTTTGTGGAG GACACCTGTC GCCTGGCCCT GGTGTACTGC AGCCTTATAA 
     3061 AGGCCCGGGC CCGCGAGCTC TCTTCAGGCC AGAAGGACCA AGGCCAGGCA GCCAACATGC 
     3121 TGTGTGTGGT GGTGAATGAC ATGGAGCAGC TGCGGCTGGT GATCGGCAAG TTGCCCGCCC 
     3181 AGCTGGCATG GGAGGCCCTG GAGCAGCGGG TAGGGGCCGT GCTGGAGCAG GGGCAGCTGC 
     3241 AGAACACGCT GCATGCCCAG CTGCAGAGCG CGCTGGCCGG GCTGGGCCAT GAGATCCGCA 
     3301 CTGGCGTCCG CACCCTGGCC GAGCAGTTGG AGGTGGGCAT CGCCAAGCAC ATCCAGAAAC 
     3361 TGGTGGGCGT CAGGGAGTCT GTCCTGCCTG AGGATGCCAT TCTGCCCCTG ATGAAGTTCC 
     3421 TGGAGGTGGA GCTTTGCTAC ATGAACACCA ACTTGGTGCA GGAGAACTTC AGCAGCCTCC 
     3481 TGACCCTGCT CTGGACCCAC ACACTCACAG TGCTGGTGGA GGCGGCCGCC TCCCAGCGCA 
     3541 GCTCATCCCT GGCTTCCAAC AGGCTGAAGA TTGCCCTGCA GAACCTGGAG ATCTGCTTCC 
     3601 ACGCTGAGGG CTGTGGCCTG CCACCCAAGG CCCTGCACAC TGCCACCTTC CAGGCTCTGC 
     3661 AGAGGGACCT GGAGCTGCAG GCGGCCTCCA GCCGGGAACT CATCCGGAAG TACTTCTGCA 
     3721 GCCGAATCCA GCAGCAGGCA GAAACCACCT CTGAGGAGCT GGGGGCTGTG ACAGTCAAGG 
     3781 CCTCCTACCG CGCCTCTGAG CAGAAGCTGC GTGTGGAGCT GCTCAGCGCC TCCAGCCTGC 
     3841 TGCCCCTGGA CTCCAATGGC TCCAGCGACC CCTTTGTCCA GCTGACCTTG GAGCCCAGGC 
     3901 ATGAGTTCCC TGAGCTGGCC GCCCGGGAGA CCCAGAAGCA CAAGAAGGAC CTTCACCCAT 
     3961 TGTTTGATGA GACCTTTGAA TTCCTGGTGC CTGCTGAGCC GTGCCGCAAG GCTGGGGCAT 
     4021 GCCTCCTGCT CACCGTGCTG GACTACGACA CGCTGGGGGC CGACGACCTG GAAGGCGAGG 
     4081 CCTTCCTGCC GCTGCGTGAG GTGCCCGGGC TGAGTGGCTC TGAGGAGCCT GGTGAGGTGC 
     4141 CTCAGACCCG CCTGCCCCTC ACGTACCCCG CACCCAACGG GGACCCAATC CTGCAGCTGC 
     4201 TGGAGGGCCG GAAGGGTGAC CGAGAAGCCC AGGTCTTTGT GAGGCTGCGG CGGCACCGGG 
     4261 CCAAGCAGGC CTCCCAGCAT GCCTTGCGGC CGGCACCGAC GCGTACGCGG CCGCTCGAGC 
     4321 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4381 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4441 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4501 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4561 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4621 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4681 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4741 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4801 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4861 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4921 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4981 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5041 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5161 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5221 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5281 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5341 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5461 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5521 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5581 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5641 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5761 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5821 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5881 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5941 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6001 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6061 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6121 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6181 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6241 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6301 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6361 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6421 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6481 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6541 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6601 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6661 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6721 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6781 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6841 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6901 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6961 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7021 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7081 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7141 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7201 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7261 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7321 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7381 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7501 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7561 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7621 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7681 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7741 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7801 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7861 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7921 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7981 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8041 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8101 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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