LOCUS       RC209137                6651 bp    DNA     circular  SYN 
DEFINITION  GRK5 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 5 (GRK5)
ACCESSION   NM_005308
VERSION     NM_005308.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2808..2899 
                     /label="Myc-DDK"  
     misc_feature    2929..3514 
                     /label="PolyA signal"  
     misc_feature    2986..3005 
                     /label="Restriction Enzyme"  
     misc_feature    3790..4462 
                     /label="ColE1"  
     misc_feature    4892..5686 
                     /label="Kan/Neo"  
     misc_feature    5721..6023 
                     /label="SV40 ori"  
     misc_feature    6109..6586 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2790..2796 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2790 
                     /label="GRK5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGGAA AACATCGTGG CCAACACGGT CTTGCTGAAA GCCAGGGAAG 
     1081 GGGGCGGAGG AAAGCGCAAA GGGAAAAGCA AGAAGTGGAA AGAAATCCTG AAGTTCCCTC 
     1141 ACATTAGCCA GTGTGAAGAC CTCCGAAGGA CCATAGACAG AGATTACTGC AGTTTATGTG 
     1201 ACAAGCAGCC AATCGGGAGG CTGCTTTTCC GGCAGTTTTG TGAAACCAGG CCTGGGCTGG 
     1261 AGTGTTACAT TCAGTTCCTG GACTCCGTGG CAGAATATGA AGTTACTCCA GATGAAAAAC 
     1321 TGGGAGAGAA AGGGAAGGAA ATTATGACCA AGTACCTCAC CCCAAAGTCC CCTGTTTTCA 
     1381 TAGCCCAAGT TGGCCAAGAC CTGGTCTCCC AGACGGAGGA GAAGCTCCTA CAGAAGCCGT 
     1441 GCAAAGAACT CTTTTCTGCC TGTGCACAGT CTGTCCACGA GTACCTGAGG GGAGAACCAT 
     1501 TCCACGAATA TCTGGACAGC ATGTTTTTTG ACCGCTTTCT CCAGTGGAAG TGGTTGGAAA 
     1561 GGCAACCGGT GACCAAAAAC ACTTTCAGGC AGTATCGAGT GCTAGGAAAA GGGGGCTTCG 
     1621 GGGAGGTCTG TGCCTGCCAG GTTCGGGCCA CGGGTAAAAT GTATGCCTGC AAGCGCTTGG 
     1681 AGAAGAAGAG GATCAAAAAG AGGAAAGGGG AGTCCATGGC CCTCAATGAG AAGCAGATCC 
     1741 TCGAGAAGGT CAACAGTCAG TTTGTGGTCA ACCTGGCCTA TGCCTACGAG ACCAAGGATG 
     1801 CACTGTGCTT GGTCCTGACC ATCATGAATG GGGGTGACCT GAAGTTCCAC ATCTACAACA 
     1861 TGGGCAACCC TGGCTTCGAG GAGGAGCGGG CCTTGTTTTA TGCGGCAGAG ATCCTCTGCG 
     1921 GCTTAGAAGA CCTCCACCGT GAGAACACCG TCTACCGAGA TCTGAAACCT GAAAACATCC 
     1981 TGTTAGATGA TTATGGCCAC ATTAGGATCT CAGACCTGGG CTTGGCTGTG AAGATCCCCG 
     2041 AGGGAGACCT GATCCGCGGC CGGGTGGGCA CTGTTGGCTA CATGGCTCCA GAGGTCCTGA 
     2101 ACAACCAGAG GTACGGCCTG AGCCCCGACT ACTGGGGCCT TGGCTGCCTC ATCTATGAGA 
     2161 TGATCGAGGG CCAGTCGCCG TTCCGCGGCC GCAAGGAGAA GGTGAAGCGG GAGGAGGTGG 
     2221 ACCGCCGGGT CCTGGAGACG GAGGAGGTGT ACTCCCACAA GTTCTCCGAG GAGGCCAAGT 
     2281 CCATCTGCAA GATGCTGCTC ACGAAAGATG CGAAGCAGAG GCTGGGCTGC CAGGAGGAGG 
     2341 GGGCTGCAGA GGTCAAGAGA CACCCCTTCT TCAGGAACAT GAACTTCAAG CGCTTAGAAG 
     2401 CCGGGATGTT GGACCCTCCC TTCGTTCCAG ACCCCCGCGC TGTGTACTGT AAGGACGTGC 
     2461 TGGACATCGA GCAGTTCTCC ACTGTGAAGG GCGTCAATCT GGACCACACA GACGACGACT 
     2521 TCTACTCCAA GTTCTCCACG GGCTCTGTGT CCATCCCATG GCAAAACGAG ATGATAGAAA 
     2581 CAGAATGCTT TAAGGAGCTG AACGTGTTTG GACCTAATGG TACCCTCCCG CCAGATCTGA 
     2641 ACAGAAACCA CCCTCCGGAA CCGCCCAAGA AAGGGCTGCT CCAGAGACTC TTCAAGCGGC 
     2701 AGCATCAGAA CAATTCCAAG AGTTCGCCCA GCTCCAAGAC CAGTTTTAAC CACCACATAA 
     2761 ACTCAAACCA TGTCAGCTCG AACTCCACCG GAAGCAGCAC GCGTACGCGG CCGCTCGAGC 
     2821 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     2881 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     2941 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3001 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3061 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3121 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3181 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3241 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3301 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3361 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3421 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3481 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3541 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3601 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3661 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     3721 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     3781 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     3841 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     3901 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     3961 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4021 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4081 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4141 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4201 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4261 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4321 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4381 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4441 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4501 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4561 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4621 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4681 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     4741 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     4801 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     4861 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     4921 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     4981 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5041 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5101 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5161 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5221 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5281 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5341 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5401 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5461 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5521 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5581 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5641 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     5701 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     5761 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     5821 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     5881 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     5941 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6001 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6061 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6121 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6181 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6241 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6301 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6361 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6421 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6481 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6541 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6601 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//