LOCUS       RC209096                8850 bp    DNA     circular  SYN 
DEFINITION  PASK (Myc-DDK-tagged)-Human PAS domain containing serine/threonine kinase (PASK)
ACCESSION   NM_015148
VERSION     NM_015148.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5007..5098 
                     /label="Myc-DDK"  
     misc_feature    5128..5713 
                     /label="PolyA signal"  
     misc_feature    5185..5204 
                     /label="Restriction Enzyme"  
     misc_feature    5989..6661 
                     /label="ColE1"  
     misc_feature    7091..7885 
                     /label="Kan/Neo"  
     misc_feature    7920..8222 
                     /label="SV40 ori"  
     misc_feature    8308..8785 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4989..4995 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4989 
                     /label="PASK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGACGGG GGCTTAACAG CCTTTGAAGA GGACCAGAGA TGCCTTTCCC 
     1081 AGAGCCTCCC CTTGCCAGTG TCAGCAGAGG GCCCAGCTGC ACAGACCACT GCTGAGCCCA 
     1141 GCAGGTCGTT TTCCTCAGCC CACAGACACC TGAGCAGAAG GAATGGGCTT TCCAGACTCT 
     1201 GCCAGAGCAG GACAGCGCTC TCTGAAGACA GATGGAGCTC CTATTGTCTA TCATCACTGG 
     1261 CTGCCCAGAA TATTTGTACA AGTAAACTGC ACTGCCCTGC TGCCCCTGAG CACACGGACC 
     1321 CGTCCGAACC GCGGGGCAGT GTGTCCTGCT GCTCCCTGCT GCGGGGACTG TCCTCAGGGT 
     1381 GGTCCTCACC TCTGCTTCCG GCCCCTGTGT GCAACCCTAA CAAGGCCATC TTCACGGTGG 
     1441 ATGCCAAGAC CACAGAGATC CTGGTTGCTA ACGACAAAGC TTGCGGGCTC CTGGGGTACA 
     1501 GCAGCCAGGA CCTGATTGGC CAGAAGCTCA CGCAGTTCTT TCTGAGGTCA GATTCTGATG 
     1561 TGGTGGAGGC CCTCAGCGAG GAGCACATGG AGGCCGACGG CCACGCTGCG GTGGTGTTTG 
     1621 GCACGGTGGT GGACATCATC AGCCGTAGTG GGGAGAAGAT TCCAGTGTCT GTGTGGATGA 
     1681 AGAGGATGCG GCAGGAGCGC CGCCTATGCT GCGTGGTGGT CCTGGAGCCC GTGGAGAGGG 
     1741 TCTCGACCTG GGTCGCTTTC CAGAGCGATG GCACCGTCAC GTCATGTGAC AGTCTCTTTG 
     1801 CTCATCTTCA CGGGTACGTG TCTGGGGAGG ACGTGGCTGG GCAGCATATC ACAGACCTGA 
     1861 TCCCTTCTGT GCAGCTCCCT CCTTCTGGCC AGCACATCCC AAAGAATCTC AAGATTCAGA 
     1921 GGTCTGTTGG AAGAGCCAGG GACGGTACCA CCTTCCCTCT GAGCTTAAAG CTGAAATCCC 
     1981 AACCCAGCAG CGAGGAGGCG ACCACCGGTG AGGCGGCCCC TGTGAGCGGC TACCGGGCAT 
     2041 CTGTCTGGGT GTTCTGCACC ATCAGTGGCC TCATCACCCT CCTGCCGGAT GGGACCATCC 
     2101 ACGGCATCAA CCACAGCTTC GCGCTGACAC TGTTTGGTTA CGGAAAGACG GAGCTCCTGG 
     2161 GCAAGAATAT CACTTTCCTG ATTCCTGGTT TCTACAGCTA CATGGACCTT GCGTACAACA 
     2221 GCTCATTACA GCTCCCAGAC CTGGCCAGCT GCCTGGACGT CGGCAATGAG AGTGGGTGTG 
     2281 GGGAGAGAAC CTTGGACCCG TGGCAGGGCC AGGACCCAGC TGAGGGGGGC CAGGATCCAA 
     2341 GGATTAATGT CGTGCTTGCT GGTGGCCACG TTGTGCCCCG AGATGAGATC CGGAAGCTGA 
     2401 TGGAAAGCCA AGACATCTTC ACCGGGACTC AGACTGAGCT GATTGCTGGA GGCCAGCTCC 
     2461 TTTCCTGCCT CTCACCTCAG CCTGCTCCAG GGGTGGACAA TGTCCCAGAA GGAAGCCTGC 
     2521 CAGTGCACGG TGAACAGGCG CTGCCCAAGG ACCAGCAAAT CACTGCCTTG GGGAGAGAGG 
     2581 AACCTGTGGC AATAGAGAGC CCCGGACAGG ATCTTCTGGG AGAAAGCAGG TCTGAACCAG 
     2641 TGGATGTGAA GCCATTTGCT TCCTGCGAAG ATTCTGAAGC TCCAGTCCCA GCTGAGGATG 
     2701 GGGGCAGTGA TGCTGGCATG TGTGGCCTGT GTCAGAAGGC CCAGCTAGAG CGGATGGGAG 
     2761 TCAGTGGTCC CAGCGGTTCA GACCTTTGGG CTGGGGCTGC CGTGGCCAAG CCCCAGGCCA 
     2821 AGGGTCAGCT GGCGGGGGGC AGCCTCCTGA TGCACTGCCC TTGCTATGGG AGTGAATGGG 
     2881 GCTTGTGGTG GCGAAGCCAG GACTTGGCCC CCAGCCCCTC TGGGATGGCA GGCCTCTCGT 
     2941 TTGGGACACC TACTCTAGAT GAGCCGTGGC TGGGAGTGGA AAACGACCGA GAAGAGCTGC 
     3001 AGACCTGCTT GATTAAGGAG CAGCTGTCCC AGTTGAGCCT TGCAGGAGCC CTGGATGTCC 
     3061 CCCACGCCGA ACTCGTTCCG ACAGAGTGCC AGGCTGTCAC CGCTCCTGTG TCGTCCTGCG 
     3121 ATCTGGGAGG CAGAGACCTG TGCGGTGGCT GCACGGGCAG CTCCTCAGCC TGCTATGCCT 
     3181 TGGCCACGGA CCTCCCTGGG GGCCTGGAAG CAGTGGAGGC CCAGGAGGTT GATGTGAATT 
     3241 CGTTTTCCTG GAACCTCAAG GAACTCTTTT TCAGTGACCA GACAGACCAA ACGTCATCAA 
     3301 ATTGTTCCTG TGCTACGTCT GAACTCAGAG AGACACCCTC TTCCTTGGCA GTGGGCTCCG 
     3361 ATCCAGATGT AGGCAGTCTC CAGGAACAGG GGTCGTGTGT CCTGGATGAC AGGGAGCTGT 
     3421 TACTACTGAC CGGCACCTGT GTTGACCTTG GCCAAGGCCG ACGGTTCCGG GAGAGCTGTG 
     3481 TGGGACATGA TCCAACAGAA CCGCTTGAGG TTTGTTTGGT GTCCTCTGAG CATTATGCAG 
     3541 CAAGCGACAG AGAAAGCCCA GGACACGTTC CTTCCACGTT GGATGCTGGC CCTGAGGACA 
     3601 CGTGCCCATC AGCAGAGGAG CCAAGGCTGA ACGTCCAGGT CACCTCCACG CCCGTGATCG 
     3661 TGATGCGCGG GGCTGCTGGC CTGCAGCGGG AGATCCAGGA GGGTGCCTAC TCCGGGAGCT 
     3721 GCTACCATCG AGATGGCTTA CGGCTGAGTA TACAGTTTGA GGTGAGGCGG GTGGAGCTCC 
     3781 AGGGCCCCAC ACCTCTGTTC TGCTGCTGGC TGGTGAAAGA CCTCCTCCAC AGCCAACGCG 
     3841 ACTCAGCCGC CAGGACCCGC CTGTTCCTTG CCAGCCTGCC CGGCTCCACC CACTCTACCG 
     3901 CTGCTGAGCT CACCGGACCC AGCCTGGTGG AAGTGCTCAG AGCCAGACCC TGGTTTGAGG 
     3961 AGCCCCCCAA GGCTGTGGAA CTGGAGGGGT TGGCGGCCTG TGAGGGCGAG TACTCCCAAA 
     4021 AGTACAGTAC CATGAGCCCG CTGGGCAGTG GGGCCTTCGG CTTCGTGTGG ACTGCTGTGG 
     4081 ACAAGGAAAA AAACAAGGAG GTGGTGGTGA AGTTTATTAA GAAGGAGAAG GTCTTGGAGG 
     4141 ATTGTTGGAT TGAGGATCCC AAACTTGGGA AAGTTACTTT AGAGATCGCA ATTCTATCCA 
     4201 GGGTGGAGCA CGCCAATATC ATCAAGGTAT TGGATATATT TGAAAACCAA GGGTTCTTCC 
     4261 AGCTTGTGAT GGAGAAGCAC GGCTCCGGCC TAGACCTCTT CGCTTTCATC GACCGCCACC 
     4321 CCAGGCTGGA TGAGCCCCTG GCGAGCTACA TCTTCCGACA ACTAGTGTCA GCAGTGGGAT 
     4381 ACCTGCGCTT GAAGGACATC ATCCACCGTG ACATCAAGGA TGAGAACATC GTGATCGCCG 
     4441 AGGACTTCAC AATCAAGCTG ATAGACTTTG GCTCGGCCGC CTACTTGGAA AGGGGAAAAT 
     4501 TATTTTATAC TTTTTGTGGG ACCATCGAGT ACTGTGCACC GGAAGTTCTC ATGGGGAATC 
     4561 CCTACAGAGG GCCGGAGCTG GAGATGTGGT CTCTGGGAGT CACTCTGTAC ACGCTGGTCT 
     4621 TTGAGGAGAA CCCCTTCTGT GAGCTGGAGG AGACCGTGGA GGCTGCCATA CACCCGCCAT 
     4681 ACCTGGTGTC CAAAGAACTC ATGAGCCTTG TGTCTGGGCT GCTGCAGCCA GTCCCTGAGA 
     4741 GACGCACCAC CTTGGAGAAG CTGGTGACAG ACCCGTGGGT AACACAGCCT GTGAATCTTG 
     4801 CTGACTATAC ATGGGAAGAG GTGTTTCGAG TAAACAAGCC AGAAAGTGGA GTTCTGTCCG 
     4861 CTGCGAGCCT GGAGATGGGG AACAGGAGCC TGAGTGATGT GGCCCAGGCT CAGGAGCTTT 
     4921 GTGGGGGCCC CGTTCCAGGC GAGGCTCCTA ATGGCCAAGG CTGTTTGCAT CCCGGGGATC 
     4981 CCCGTCTGCT GACCAGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5041 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5101 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5161 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5221 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5281 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5341 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5401 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5461 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5521 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5581 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5641 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5701 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5761 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5821 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5881 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5941 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6001 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6061 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6121 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6181 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6241 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6301 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6361 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6421 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6481 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6541 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6601 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6661 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6721 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6781 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6841 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6901 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6961 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7021 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7081 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7141 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7201 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7261 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7321 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7381 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7441 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7501 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7561 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7621 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7681 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7741 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7801 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7861 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7921 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7981 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8041 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8101 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8221 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8281 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8341 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8401 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8461 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8521 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8581 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8641 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8701 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8761 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8821 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//