LOCUS       RC209051L1                9162 bp    DNA     circular  SYN 
DEFINITION  SCYL2 (Myc-DDK-tagged)-Human SCY1-like 2 (S. cerevisiae) (SCYL2)
ACCESSION   NM_017988
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7453..7633 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8361..9020 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7792..8360 
                     /label="ColE1"  
     misc_feature    6328..6348 
                     /label="LR50 primer"  
     misc_feature    6125..6196 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6337..6928 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6107..6113 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6107 
                     /label="SCYL2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGTCCATG CTTAATAAAT TGAAGAGTAC 
     3361 TGTTACAAAA GTAACAGCTG ATGTCACTAG TGCTGTAATG GGAAATCCTG TCACTAGAGA 
     3421 ATTTGATGTT GGTCGACACA TTGCCAGTGG TGGCAATGGG CTAGCTTGGA AGATTTTTAA 
     3481 TGGCACAAAA AAGTCAACAA AGCAGGAAGT GGCAGTTTTT GTCTTTGATA AAAAACTGAT 
     3541 TGACAAGTAT CAAAAATTTG AAAAGGATCA AATCATTGAT TCTCTAAAAC GAGGAGTCCA 
     3601 ACAGTTAACT CGGCTTCGAC ACCCTCGACT TCTTACTGTC CAGCATCCTT TAGAAGAATC 
     3661 CAGGGATTGC TTGGCATTTT GTACAGAACC AGTTTTTGCC AGTTTAGCCA ATGTTCTTGG 
     3721 TAACTGGGAA AATCTACCTT CCCCTATATC TCCAGACATT AAGGATTATA AACTTTATGA 
     3781 TGTAGAAACC AAATATGGTT TGCTTCAGGT TTCTGAAGGA TTGTCATTCT TGCATAGCAG 
     3841 TGTGAAAATG GTGCATGGAA ATATCACTCC TGAAAATATA ATTTTGAATA AAAGTGGAGC 
     3901 CTGGAAAATA ATGGGTTTTG ATTTTTGTGT ATCATCAACC AATCCTTCTG AACAAGAGCC 
     3961 TAAATTTCCT TGTAAAGAAT GGGACCCAAA TTTACCTTCA TTGTGTCTTC CAAATCCTGA 
     4021 ATATTTGGCT CCTGAATACA TACTTTCTGT GAGCTGTGAA ACAGCCAGTG ATATGTATTC 
     4081 TTTAGGAACT GTTATGTATG CTGTATTTAA TAAAGGGAAA CCTATATTTG AAGTCAACAA 
     4141 GCAAGATATT TACAAGAGTT TCAGTAGGCA GTTGGATCAG TTGAGTCGTT TAGGATCTAG 
     4201 TTCACTTACA AATATACCTG AGGAAGTTCG TGAACATGTA AAGCTACTGT TAAATGTAAC 
     4261 TCCGACTGTA AGACCAGATG CAGATCAAAT GACAAAGATT CCCTTCTTTG ATGATGTTGG 
     4321 TGCAGTAACA CTGCAATATT TTGATACCTT ATTCCAAAGA GATAATCTTC AGAAATCACA 
     4381 GTTTTTCAAA GGACTGCCAA AGGTTCTACC AAAACTGCCC AAGCGTGTCA TTGTGCAGAG 
     4441 AATTTTGCCT TGTTTGACTT CAGAATTTGT AAACCCTGAC ATGGTACCTT TTGTTTTGCC 
     4501 CAATGTTCTA CTTATTGCTG AGGAATGCAC CAAAGAAGAA TATGTCAAAT TAATTCTTCC 
     4561 TGAACTTGGC CCTGTGTTTA AGCAGCAGGA GCCAATCCAG ATTTTGTTAA TTTTCCTACA 
     4621 AAAAATGGAT TTGCTACTAA CCAAAACCCC TCCTGATGAG ATAAAGAACA GTGTTCTACC 
     4681 CATGGTTTAC AGAGCACTAG AAGCTCCTTC CATTCAGATC CAGGAGCTCT GTCTAAACAT 
     4741 CATTCCAACC TTTGCAAATC TTATAGACTA CCCATCCATG AAAAACGCTT TGATACCAAG 
     4801 AATTAAAAAT GCTTGTCTAC AAACATCTTC CCTTGCGGTT CGTGTAAATT CATTAGTGTG 
     4861 CTTAGGAAAG ATTTTGGAAT ACTTGGATAA GTGGTTTGTA CTTGATGATA TCCTACCCTT 
     4921 CTTACAACAA ATTCCATCCA AGGAACCTGC GGTCCTCATG GGAATTTTAG GTATTTACAA 
     4981 ATGTACTTTT ACTCATAAGA AGTTGGGAAT CACCAAAGAG CAGCTGGCCG GAAAAGTGTT 
     5041 GCCTCATCTT ATTCCCCTGA GTATTGAAAA CAATCTTAAT CTTAATCAGT TCAATTCTTT 
     5101 CATTTCCGTC ATAAAAGAAA TGCTTAATAG ATTGGAGTCT GAACATAAGA CTAAACTGGA 
     5161 GCAACTTCAT ATAATGCAAG AACAGCAGAA ATCTTTGGAT ATAGGAAATC AAATGAATGT 
     5221 TTCTGAGGAG ATGAAAGTTA CAAATATTGG GAATCAGCAA ATTGACAAAG TTTTTAACAA 
     5281 CATTGGAGCA GACCTTCTGA CTGGCAGTGA GTCCGAAAAT AAAGAGGACG GGTTACAGAA 
     5341 TAAACATAAA AGAGCATCAC TTACACTTGA AGAAAAACAA AAATTAGCAA AAGAACAAGA 
     5401 GCAGGCACAG AAGCTGAAAA GCCAGCAGCC TCTTAAACCC CAAGTGCACA CACCTGTTGC 
     5461 TACTGTTAAA CAGACTAAGG ACTTGACAGA CACACTGATG GATAATATGT CATCCTTGAC 
     5521 CAGCCTTTCT GTTAGTACCC CTAAATCTTC TGCTTCAAGT ACTTTCACTT CTGTTCCTTC 
     5581 CATGGGCATT GGTATGATGT TTTCTACACC AACTGATAAT ACAAAGAGAA ATTTGACAAA 
     5641 TGGCCTAAAT GCCAATATGG GCTTTCAGAC TTCAGGATTC AACATGCCCG TTAATACAAA 
     5701 CCAGAACTTC TACAGTAGTC CAAGCACAGT TGGAGTGACC AAGATGACTC TGGGAACACC 
     5761 TCCCACTTTG CCAAACTTCA ATGCTTTGAG TGTTCCTCCT GCTGGTGCAA AGCAGACCCA 
     5821 ACAAAGACCC ACAGATATGT CTGCCCTTAA TAATCTCTTT GGCCCTCAGA AACCCAAAGT 
     5881 TAGCATGAAC CAGTTATCAC AACAGAAACC AAATCAGTGG CTTAATCAGT TTGTACCTCC 
     5941 TCAAGGTTCT CCAACTATGG GCAGTTCAGT AATGGGGACA CAGATGAACG TGATAGGACA 
     6001 ATCTGCTTTT GGTATGCAGG GTAATCCTTT CTTTAACCCA CAGAACTTTG CACAGCCACC 
     6061 AACTACTATG ACCAATAGCA GTTCAGCTAG CAATGATTTA AAAGATCTTT TTGGGACGCG 
     6121 TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT 
     6181 GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCTGT ACAAGTAGGA 
     6241 TTCGTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ACATGTTTAA 
     6301 GGGTTCCGGT TCCACTAGGT ACAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT 
     6361 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 
     6421 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 
     6481 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 
     6541 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 
     6601 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 
     6661 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 
     6721 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC 
     6781 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 
     6841 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 
     6901 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC TCGATCGAGA 
     6961 CCTAGAAAAA CATGGAGCAA TCACAAGTAG CAATACAGCA GCTACCAATG CTGATTGTGC 
     7021 CTGGCTAGAA GCACAAGAGG AGGAGGAGGT GGGTTTTCCA GTCACACCTC AGGTACCTTT 
     7081 AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG 
     7141 ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATATC CTTGATCTGT GGATCTACCA 
     7201 CACACAAGGC TACTTCCCTG ATTGGCAGAA CTACACACCA GGGCCAGGGA TCAGATATCC 
     7261 ACTGACCTTT GGATGGTGCT ACAAGCTAGT ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC 
     7321 CAATGAAGGA GAGAACACCC GCTTGTTACA CCCTGTGAGC CTGCATGGGA TGGATGACCC 
     7381 GGAGAGAGAA GTATTAGAGT GGAGGTTTGA CAGCCGCCTA GCATTTCATC ACATGGCCCG 
     7441 AGAGCTGCAT CCGGACTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 
     7501 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 
     7561 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 
     7621 GTCAGTGTGG AAAATCTCTA GCAGCATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     7681 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     7741 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     7801 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     7861 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     7921 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     7981 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     8041 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     8101 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     8161 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     8221 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     8281 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     8341 AAACTCACGT TAAGGGATTT TGGTCATGAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC 
     8401 TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC 
     8461 TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA 
     8521 ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC 
     8581 CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG 
     8641 TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG 
     8701 TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA 
     8761 GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAACTCCGGA 
     8821 TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT 
     8881 TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT 
     8941 TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG 
     9001 GTGGTATATC CAGTGATTTT TTTCTCCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     9061 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     9121 ATAGGGGTCC CGCGCACATT TCCCCGAAAA GTGCCACCTG AC
//