LOCUS       RC208984                8271 bp    DNA     circular  SYN 
DEFINITION  PELP1 (Myc-DDK-tagged)-Human proline, glutamate and leucine rich protein 1 (PELP1)
ACCESSION   NM_014389
VERSION     NM_014389.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="PELP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCAGCC GTTCTGAGTG GGCCCTCTGC GGGCTCCGCG GCTGGGGTTC 
     1081 CTGGCGGGAC CGGGGGTCTC TCGGCAGTGA GCTCGGGCCC GCGGCTCCGC CTGCTGCTGC 
     1141 TGGAGAGTGT TTCTGGTTTG CTGCAACCTC GAACGGGGTC TGCCGTTGCT CCGGTGCATC 
     1201 CCCCAAACCG CTCGGCCCCA CATTTGCCCG GGCTCATGTG CCTATTGCGG CTGCATGGGT 
     1261 CGGTGGGCGG GGCCCAGAAC CTTTCAGCTC TTGGGGCATT GGTGAGTCTC AGTAATGCAC 
     1321 GTCTCAGTTC CATCAAAACT CGGTTTGAGG GCCTGTGTCT GCTGTCCCTG CTGGTAGGGG 
     1381 AGAGCCCCAC AGAGCTATTC CAGCAGCACT GTGTGTCTTG GCTTCGGAGC ATTCAGCAGG 
     1441 TGTTACAGAC CCAGGACCCG CCTGCCACAA TGGAGCTGGC CGTGGCTGTC CTGAGGGACC 
     1501 TCCTCCGATA TGCAGCCCAG CTGCCTGCAC TGTTCCGGGA CATCTCCATG AACCACCTCC 
     1561 CTGGCCTTCT CACCTCCCTG CTGGGCCTCA GGCCAGAGTG TGAGCAGTCA GCATTGGAAG 
     1621 GAATGAAGGC TTGTATGACC TATTTCCCTC GGGCTTGTGG TTCTCTCAAA GGCAAGCTGG 
     1681 CCTCATTTTT TCTGTCTAGG GTGGATGCCT TGAGCCCTCA GCTCCAACAG TTGGCCTGTG 
     1741 AGTGTTATTC CCGGCTGCCC TCTTTAGGGG CTGGCTTTTC CCAAGGCCTG AAGCACACCG 
     1801 AGAGCTGGGA GCAGGAGCTA CACAGTCTGC TGGCCTCACT GCACACCCTG CTGGGGGCCC 
     1861 TGTACGAGGG AGCAGAGACT GCTCCTGTGC AGAATGAAGG CCCTGGGGTG GAGATGCTGC 
     1921 TGTCCTCAGA AGATGGTGAT GCCCATGTCC TTCTCCAGCT TCGGCAGAGG TTTTCGGGAC 
     1981 TGGCCCGCTG CCTAGGGCTC ATGCTCAGCT CTGAGTTTGG AGCTCCCGTG TCCGTCCCTG 
     2041 TGCAGGAAAT CCTGGATTTC ATCTGCCGGA CCCTCAGCGT CAGTAGCAAG AATATTAGCT 
     2101 TGCATGGAGA TGGTCCCCTG CGGCTGCTGC TGCTGCCCTC TATCCACCTT GAGGCCTTGG 
     2161 ACCTGCTGTC TGCACTCATC CTCGCGTGTG GAAGCCGGCT CTTGCGCTTT GGGATCCTGA 
     2221 TCGGCCGCCT GCTTCCCCAG GTCCTCAATT CCTGGAGCAT CGGTAGAGAT TCCCTCTCTC 
     2281 CAGGCCAGGA GAGGCCTTAC AGCACGGTTC GGACCAAGGT GTATGCGATA TTAGAGCTGT 
     2341 GGGTGCAGGT TTGTGGGGCC TCGGCGGGAA TGCTTCAGGG AGGAGCCTCT GGAGAGGCCC 
     2401 TGCTCACCCA CCTGCTCAGC GACATCTCCC CGCCAGCTGA TGCCCTTAAG CTGCGTAGCC 
     2461 CGCGGGGGAG CCCTGATGGG AGTTTGCAGA CTGGGAAGCC TAGCGCCCCC AAGAAGCTAA 
     2521 AGCTGGATGT GGGGGAAGCT ATGGCCCCGC CAAGCCACCG GAAAGGGGAT AGCAATGCCA 
     2581 ACAGCGACGT GTGTGCGGCT GCACTCAGAG GCCTCAGCCG GACCATCCTC ATGTGTGGGC 
     2641 CTCTCATCAA GGAGGAGACT CACAGGAGAC TGCATGACCT GGTCCTCCCC CTGGTCATGG 
     2701 GTGTACAGCA GGGTGAGGTC CTAGGCAGCT CCCCGTACAC GAGCTCCCGC TGCCGCCGTG 
     2761 AACTCTACTG CCTGCTGCTG GCGCTGCTGC TGGCCCCGTC TCCTCGCTGC CCACCTCCTC 
     2821 TTGCCTGTGC CCTGCAAGCC TTCTCCCTCG GCCAGCGAGA AGATAGCCTT GAGGTCTCCT 
     2881 CTTTCTGCTC AGAAGCACTG GTGACCTGTG CTGCTCTGAC CCACCCCCGG GTTCCTCCCC 
     2941 TGCAGCCCAT GGGCCCCACC TGCCCCACAC CTGCTCCAGT TCCCCCTCCT GAGGCCCCAT 
     3001 CGCCCTTCAG GGCCCCACCG TTCCATCCTC CGGGCCCCAT GCCCTCAGTG GGCTCCATGC 
     3061 CCTCAGCAGG CCCCATGCCT TCAGCAGGCC CCATGCCCTC AGCAGGCCCT GTGCCCTCGG 
     3121 CACGCCCTGG ACCTCCCACC ACAGCCAACC ACCTAGGCCT TTCTGTCCCA GGCCTAGTGT 
     3181 CTGTCCCTCC CCGGCTTCTT CCTGGCCCTG AGAACCACCG GGCAGGCTCA AATGAGGACC 
     3241 CCATCCTTGC CCCTAGTGGG ACTCCCCCAC CTACTATACC CCCAGATGAA ACTTTTGGGG 
     3301 GGAGAGTGCC CAGACCAGCC TTTGTCCACT ATGACAAGGA GGAGGCATCT GATGTGGAGA 
     3361 TCTCCTTGGA AAGTGACTCT GATGACAGCG TGGTGATCGT GCCCGAGGGG CTTCCCCCCC 
     3421 TGCCACCCCC ACCACCCTCA GGTGCCACAC CACCCCCTAT AGCCCCCACT GGGCCACCAA 
     3481 CAGCCTCCCC TCCTGTGCCA GCGAAGGAGG AGCCTGAAGA ACTTCCTGCA GCCCCAGGGC 
     3541 CTCTCCCGCC ACCCCCACCT CCGCCGCCGC CTGTTCCTGG TCCTGTGACG CTCCCTCCAC 
     3601 CCCAGTTGGT CCCTGAAGGG ACTCCTGGTG GGGGAGGACC CCCAGCCCTG GAAGAGGATT 
     3661 TGACAGTTAT TAATATCAAC AGCAGTGATG AAGAGGAGGA GGAAGAGGAA GAAGAGGAAG 
     3721 AAGAAGAAGA GGAAGAAGAG GAAGAGGAGG AAGACTTTGA GGAAGAGGAA GAGGATGAAG 
     3781 AGGAATATTT TGAAGAGGAA GAAGAGGAGG AAGAAGAGTT TGAGGAAGAA TTTGAGGAAG 
     3841 AAGAAGGTGA GTTAGAGGAA GAAGAAGAAG AGGAGGATGA GGAGGAGGAA GAAGAACTGG 
     3901 AAGAGGTGGA AGACCTGGAG TTTGGCACAG CAGGAGGGGA GGTAGAAGAA GGTGCACCTC 
     3961 CACCCCCAAC CCTGCCTCCA GCTCTGCCTC CCCCTGAGTC TCCCCCAAAG GTGCAGCCAG 
     4021 AACCCGAACC TGAACCCGGG CTGCTTTTGG AAGTGGAGGA GCCAGGGACG GAGGAGGAGC 
     4081 GTGGGGCTGA CACAGCTCCC ACCCTGGCCC CTGAAGCGCT CCCCTCCCAG GGAGAGGTGG 
     4141 AGAGGGAAGG GGAAAGCCCT GCGGCAGGGC CCCCTCCCCA GGAGCTTGTT GAAGAAGAGC 
     4201 CCTCTGCTCC CCCAACCCTG TTGGAAGAGG AGACTGAGGA TGGGAGTGAC AAGGTGCAGC 
     4261 CCCCACCAGA GACACCTGCA GAAGAAGAGA TGGAGACAGA GACAGAGGCC GAAGCTCTCC 
     4321 AGGAAAAGGA GCAGGATGAC ACAGCTGCCA TGCTGGCCGA CTTCATCGAT TGTCCCCCTG 
     4381 ATGATGAGAA GCCACCACCT CCCACAGAGC CTGACTCCAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//