LOCUS       RC208942                8313 bp    DNA     circular  SYN 
DEFINITION  SREBF2 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 2 (SREBF2)
ACCESSION   NM_004599
VERSION     NM_004599.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4468..4559 
                     /label="Myc-DDK"  
     misc_feature    4589..5174 
                     /label="PolyA signal"  
     misc_feature    4646..4665 
                     /label="Restriction Enzyme"  
     misc_feature    5450..6122 
                     /label="ColE1"  
     misc_feature    6552..7346 
                     /label="Kan/Neo"  
     misc_feature    7381..7683 
                     /label="SV40 ori"  
     misc_feature    7769..8246 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4443..4450 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4443 
                     /label="SREBF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGACAGC GGCGAGCTGG GTGGTCTGGA GACCATGGAG ACCCTCACGG 
     1081 AGCTGGGCGA CGAGCTGACC CTGGGAGACA TCGACGAGAT GCTGCAATTT GTCAGTAATC 
     1141 AAGTGGGAGA GTTCCCTGAC TTGTTTTCAG AACAGCTGTG TAGCTCCTTT CCTGGCAGTG 
     1201 GTGGTAGTGG TAGCAGCAGC GGCAGCAGTG GCAGCAGCAG CAGCAGCAGC AATGGCAGGG 
     1261 GCAGCAGCAG CGGAGCTGTG GACCCTTCAG TGCAACGGTC ATTCACCCAG GTCACATTAC 
     1321 CTTCCTTCTC TCCCTCGGCG GCCTCCCCAC AGGCTCCAAC TCTGCAAGTC AAGGTTTCTC 
     1381 CCACCTCAGT TCCCACCACA CCCAGGGCAA CTCCTATTCT TCAGCCCCGC CCCCAGCCCC 
     1441 AGCCTCAACC TCAAACTCAG CTGCAACAAC AGACGGTAAT GATCACGCCA ACATTCAGCA 
     1501 CCACTCCGCA GACGAGGATC ATCCAGCAGC CTTTGATATA CCAGAATGCA GCTACTAGCT 
     1561 TTCAAGTCCT TCAGCCTCAA GTCCAAAGCC TGGTGACATC CTCCCAGGTA CAGCCGGTCA 
     1621 CCATTCAGCA GCAGGTGCAG ACAGTACAGG CCCAGCGGGT GCTGACACAA ACGGCCAATG 
     1681 GCACGCTGCA GACCCTTGCC CCGGCTACGG TGCAGACAGT TGCTGCGCCA CAGGTGCAGC 
     1741 AGGTCCCGGT CCTGGTCCAG CCTCAGATCA TCAAGACAGA TTCCCTTGTT TTGACCACAC 
     1801 TGAAGACAGA TGGCAGCCCT GTTATGGCTG CGGTCCAGAA CCCGGCCCTC ACCGCCCTCA 
     1861 CCACCCCTAT CCAGACGGCT GCCCTTCAAG TACCAACCCT GGTGGGCAGC AGTGGGACCA 
     1921 TTCTGACCAC AATGCCTGTA ATGATGGGGC AAGAGAAAGT GCCCATTAAG CAGGTACCTG 
     1981 GGGGAGTCAA GCAGCTTGAG CCCCCCAAAG AAGGAGAAAG GCGGACAACC CATAATATCA 
     2041 TTGAGAAACG ATATCGCTCC TCCATCAATG ACAAAATCAT CGAATTGAAA GACCTGGTCA 
     2101 TGGGGACAGA CGCCAAGATG CACAAGTCTG GCGTTCTGAG GAAGGCCATT GATTACATCA 
     2161 AATACTTGCA GCAGGTCAAT CATAAACTGC GCCAGGAGAA CATGGTGCTG AAGCTGGCAA 
     2221 ATCAAAAGAA CAAGCTTCTA AAGGGCATCG ACCTAGGCAG TCTGGTGGAC AATGAGGTGG 
     2281 ACCTGAAGAT CGAGGACTTT AATCAGAATG TCCTTCTGAT GTCCCCCCCA GCCTCTGACT 
     2341 CAGGGTCCCA GGCTGGCTTC TCTCCCTACT CCATTGACTC TGAGCCAGGA AGCCCTCTAT 
     2401 TGGATGATGC AAAGGTCAAA GATGAGCCAG ACTCTCCTCC TGTGGCGCTG GGCATGGTAG 
     2461 ACCGCTCACG GATTCTTCTG TGTGTCCTCA CCTTCCTGTG CCTCTCCTTT AACCCCCTGA 
     2521 CTTCCCTGCT GCAGTGGGGA GGGGCCCACG ACTCTGACCA GCACCCACAC TCAGGCTCTG 
     2581 GCCGCAGTGT CCTGTCATTC GAGTCAGGTT CTGGGGGCTG GTTTGACTGG ATGATGCCTA 
     2641 CTCTTCTCTT ATGGCTGGTA AATGGTGTGA TTGTCCTGAG CGTCTTTGTG AAGCTTCTGG 
     2701 TTCATGGGGA GCCAGTGATC CGGCCACACT CGCGCTCCTC GGTCACCTTC TGGAGGCACC 
     2761 GGAAACAGGC AGATCTGGAT CTCGCCAGAG GAGATTTTGC AGCTGCTGCC GGCAACCTAC 
     2821 AAACCTGCCT GGCAGTTTTG GGCCGGGCAC TGCCCACCTC CCGCCTGGAC CTGGCCTGCA 
     2881 GCCTCTCCTG GAACGTGATC CGCTACAGCC TGCAGAAGCT ACGCCTGGTG CGCTGGCTGC 
     2941 TCAAGAAAGT CTTCCAGTGC CGGCGGGCCA CGCCAGCCAC TGAGGCAGGC TTTGAAGACG 
     3001 AAGCTAAGAC CAGCGCCCGG GATGCGGCTC TGGCCTATCA CCGGCTGCAC CAGCTGCACA 
     3061 TCACAGGGAA GCTTCCTGCA GGATCCGCCT GTTCCGATGT ACACATGGCG TTGTGTGCCG 
     3121 TGAACCTGGC TGAATGTGCA GAGGAGAAGA TCCCACCGAG CACACTGGTT GAGATCCATC 
     3181 TGACTGCTGC CATGGGGCTC AAGACCCGGT GTGGAGGCAA GCTGGGCTTC CTGGCCAGCT 
     3241 ACTTCCTCAG CCGAGCCCAG AGCCTGTGTG GCCCCGAGCA CAGTGCTGTT CCTGACTCCC 
     3301 TGCGCTGGCT CTGCCACCCC CTGGGCCAGA AGTTTTTCAT GGAGCGGAGC TGGTCTGTGA 
     3361 AGTCAGCTGC CAAGGAGAGT CTATACTGTG CCCAGAGGAA CCCAGCTGAC CCCATTGCGC 
     3421 AGGTCCACCA GGCCTTCTGC AAGAACCTGC TGGAGCGAGC TATAGAGTCC TTGGTGAAAC 
     3481 CTCAGGCCAA GAAGAAGGCT GGAGACCAGG AAGAAGAGAG CTGTGAATTC TCCAGTGCTC 
     3541 TGGAGTACTT GAAATTACTT CATTCTTTTG TGGACTCTGT GGGGGTTATG AGCCCCCCAC 
     3601 TCTCCAGGAG CTCCGTGCTC AAGTCCGCCC TGGGTCCAGA CATCATCTGT CGGTGGTGGA 
     3661 CGTCTGCAAT CACTGTGGCC ATCAGCTGGC TCCAGGGAGA CGATGCAGCT GTGCGCTCTC 
     3721 ATTTTACCAA AGTGGAACGC ATCCCCAAGG CCCTGGAAGT GACAGAGAGC CCCCTGGTGA 
     3781 AGGCCATCTT CCATGCCTGC AGAGCCATGC ATGCCTCACT CCCTGGGAAA GCAGATGGGC 
     3841 AGCAGAGTTC CTTCTGCCAT TGCGAGAGGG CCAGTGGCCA CCTATGGAGC AGCCTCAACG 
     3901 TCAGTGGGGC CACCTCTGAC CCTGCCCTCA ACCACGTGGT CCAGCTGCTC ACCTGTGACC 
     3961 TGCTACTGTC GCTACGGACA GCGCTCTGGC AAAAACAGGC CAGTGCCAGC CAGGCTGTGG 
     4021 GGGAGACCTA CCACGCGTCA GGCGCTGAAC TGGCGGGCTT CCAACGGGAC CTGGGCAGCC 
     4081 TGCGCAGGCT GGCACACAGC TTCCGCCCAG CATACCGCAA GGTGTTCCTG CATGAAGCCA 
     4141 CCGTGCGCCT GATGGCAGGA GCCAGCCCCA CCCGCACCCA CCAGCTGCTG GAACACAGCC 
     4201 TGCGGCGGCG CACCACGCAG AGCACCAAGC ACGGAGAGGT GGATGCCTGG CCCGGCCAGC 
     4261 GAGAGCGGGC CACCGCCATC CTGCTGGCCT GCCGCCACCT GCCCCTCTCC TTCCTCTCCT 
     4321 CCCCGGGCCA GCGGGCAGTG CTGCTGGCCG AAGCTGCCCG CACCCTGGAG AAGGTGGGCG 
     4381 ACCGGCGCTC CTGCAACGAC TGCCAGCAGA TGATTGTTAA GCTGGGTGGT GGCACTGCCA 
     4441 TTGCCGCCTC CAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4501 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5281 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5341 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5401 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5461 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5521 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5581 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5641 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5701 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5761 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5821 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5881 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5941 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6001 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6061 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6121 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6181 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6241 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6301 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6361 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6421 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6481 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6541 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6601 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6661 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6721 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6781 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6841 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6901 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6961 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7021 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7081 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7141 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7201 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7261 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7321 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7381 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7441 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7501 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7561 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7741 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7801 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7861 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7921 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7981 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8041 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8101 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8161 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8221 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8281 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//