LOCUS       RC208907L1                9249 bp    DNA     circular  SYN 
DEFINITION  DIS3 (Myc-DDK-tagged)-Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1
ACCESSION   NM_014953
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7540..7720 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8448..9107 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7879..8447 
                     /label="ColE1"  
     misc_feature    6415..6435 
                     /label="LR50 primer"  
     misc_feature    6212..6283 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6424..7015 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6194..6200 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6194 
                     /label="DIS3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCTCAAGTCC AAGACGTTCT TAAAAAAGAC 
     3361 CCGGGCGGGC GGCGTGATGA AGATCGTGCG CGAGCACTAC CTGCGAGACG ACATCGGCTG 
     3421 CGGTGCGCCC GGGTGCGCAG CGTGTGGAGG GGCGCACGAG GGGCCGGCCC TGGAGCCGCA 
     3481 GCCCCAGGAC CCGGCGAGCA GCGTCTGCCC GCAACCGCAC TACTTGCTGC CCGACACTAA 
     3541 TGTGTTACTG CACCAGATTG ATGTTCTTGA GGACCCTGCC ATCAGGAATG TAATTGTGCT 
     3601 ACAAACAGTT CTTCAAGAAG TGAGAAATCG CAGTGCCCCC GTATATAAAC GCATCCGAGA 
     3661 TGTGACTAAT AACCAAGAGA AGCATTTCTA TACTTTCACT AATGAGCACC ATAGAGAAAC 
     3721 CTATGTAGAA CAAGAACAGG GAGAAAATGC TAATGACAGG AATGATAGAG CGATTCGAGT 
     3781 AGCAGCAAAA TGGTACAATG AACATTTGAA AAAAATGTCA GCAGACAACC AGCTGCAAGT 
     3841 TATCTTCATA ACAAATGACA GGAGAAACAA AGAGAAAGCC ATAGAAGAAG GAATACCAGC 
     3901 TTTCACTTGT GAAGAATATG TAAAGAGCCT AACTGCTAAC CCCGAACTCA TAGATCGTCT 
     3961 TGCTTGTTTG TCTGAAGAAG GGAATGAAAT AGAAAGTGGA AAAATAATAT TTTCAGAGCA 
     4021 TCTTCCCTTA AGTAAGCTAC AGCAAGGCAT AAAATCTGGT ACATACCTTC AAGGAACATT 
     4081 TAGAGCTAGC AGGGAAAATT ACTTGGAAGC TACAGTATGG ATTCATGGCG ACAATGAAGA 
     4141 AAATAAAGAG ATAATCTTAC AGGGACTTAA ACATTTAAAC AGAGCTGTTC ACGAAGATAT 
     4201 TGTGGCTGTG GAGCTTCTCC CCAAGAGTCA GTGGGTAGCA CCATCTTCTG TGGTTTTACA 
     4261 TGATGAAGGT CAAAATGAAG AAGATGTGGA GAAAGAAGAA GAGAGAGAAC GAATGCTTAA 
     4321 GACTGCTGTA AGCGAGAAAA TGTTGAAGCC TACAGGTAGA GTTGTAGGAA TAATAAAAAG 
     4381 GAATTGGAGA CCATATTGTG GCATGCTTTC CAAGTCTGAC ATTAAGGAGT CAAGAAGACA 
     4441 TCTCTTTACA CCTGCTGATA AGAGAATCCC TCGAATTCGC ATAGAAACCA GACAGGCTTC 
     4501 CACATTAGAA GGACGGAGAA TTATTGTTGC TATTGATGGT TGGCCCAGAA ATTCCAGATA 
     4561 TCCAAATGGA CACTTTGTGA GAAATTTAGG TGATGTTGGA GAGAAAGAGA CTGAAACAGA 
     4621 AGTTTTGTTA CTTGAACACG ATGTTCCCCA TCAGCCTTTT TCACAGGCTG TTCTTAGTTT 
     4681 TCTGCCAAAG ATGCCCTGGA GCATTACTGA AAAGGACATG AAAAACCGAG AAGACCTGAG 
     4741 GCATCTGTGT ATTTGTAGTG TAGACCCACC AGGATGTACT GATATAGACG ATGCTCTACA 
     4801 TTGTCGAGAA CTCGAAAATG GAAATTTGGA GGTTGGTGTT CATATTGCTG ATGTGAGCCA 
     4861 TTTTATTAGG CCAGGAAATG CCTTGGATCA AGAATCAGCC AGAAGAGGAA CAACTGTGTA 
     4921 TCTTTGTGAA AAGAGGATTG ACATGGTTCC AGAGTTGCTT AGCTCTAACT TGTGTTCCTT 
     4981 AAAATGTGAC GTGGACAGGC TGGCATTTTC ATGTATTTGG GAAATGAATC ACAATGCTGA 
     5041 AATCTTAAAA ACGAAGTTTA CCAAAAGTGT TATTAATTCA AAGGCATCTC TGACATATGC 
     5101 TGAAGCTCAG TTGAGAATTG ATTCAGCAAA CATGAATGAT GATATTACCA CTAGTCTCCG 
     5161 TGGACTGAAT AAACTAGCCA AAATTCTGAA GAAAAGAAGG ATTGAAAAAG GGGCTTTGAC 
     5221 TCTATCCTCT CCTGAAGTTC GATTCCACAT GGACAGTGAA ACTCACGATC CTATAGATCT 
     5281 GCAGACCAAG GAACTTAGGG AAACAAATTC CATGGTTGAA GAATTTATGT TACTTGCCAA 
     5341 TATTTCTGTT GCAAAAAAAA TTCATGAGGA ATTTTCTGAA CATGCTCTGC TTCGAAAACA 
     5401 TCCTGCTCCA CCTCCATCAA ATTATGAAAT TCTTGTTAAG GCAGCCAGGT CAAGGAATTT 
     5461 GGAAATTAAG ACTGATACAG CCAAGTCTTT GGCTGAGTCT TTGGATCAGG CCGAATCTCC 
     5521 TACTTTTCCA TATCTAAACA CTCTGTTGAG AATATTAGCC ACTCGCTGTA TGATGCAAGC 
     5581 TGTGTACTTC TGTTCTGGAA TGGATAATGA TTTTCATCAC TATGGCTTAG CGTCTCCAAT 
     5641 ATACACACAT TTTACTTCAC CCATTAGAAG ATACGCAGAT GTCATTGTTC ATCGGCTTTT 
     5701 GGCTGTGGCT ATTGGGGCTG ACTGTACTTA TCCAGAGTTG ACAGACAAAC ACAAGCTTGC 
     5761 AGATATATGT AAAAATCTAA ATTTCCGGCA CAAAATGGCT CAATATGCCC AACGTGCATC 
     5821 AGTGGCTTTT CATACCCAGT TATTCTTCAA AAGCAAAGGA ATAGTAAGTG AAGAAGCCTA 
     5881 TATTTTATTT GTAAGAAAGA ATGCCATTGT GGTATTAATT CCAAAGTATG GTTTAGAAGG 
     5941 GACAGTCTTT TTTGAAGAAA AGGACAAACC AAACCCACAG CTTATTTATG ATGATGAGAT 
     6001 ACCCTCACTT AAAATAGAAG ATACAGTGTT CCATGTATTT GATAAAGTTA AAGTGAAAAT 
     6061 CATGTTAGAC TCATCTAATC TTCAACATCA GAAGATCCGA ATGTCCCTGG TAGAACCACA 
     6121 GATACCAGGA ATAAGCATTC CTACTGATAC TTCAAACATG GACCTTAATG GACCAAAGAA 
     6181 AAAGAAGATG AAGCTTGGAA AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     6241 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC 
     6301 GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT 
     6361 ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC 
     6421 AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 
     6481 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT 
     6541 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT 
     6601 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC 
     6661 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT 
     6721 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA 
     6781 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT 
     6841 CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC 
     6901 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT 
     6961 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG 
     7021 CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA 
     7081 TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG 
     7141 TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 
     7201 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA 
     7261 AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA 
     7321 CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC 
     7381 AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC 
     7441 TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG 
     7501 CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG 
     7561 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC 
     7621 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG 
     7681 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC 
     7741 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     7801 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     7861 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     7921 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     7981 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     8041 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     8101 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     8161 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     8221 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     8281 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     8341 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     8401 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC 
     8461 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG 
     8521 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT 
     8581 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG 
     8641 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA 
     8701 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT 
     8761 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA 
     8821 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG 
     8881 TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA 
     8941 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC 
     9001 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT 
     9061 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC 
     9121 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     9181 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTCCCGC GCACATTTCC CCGAAAAGTG 
     9241 CCACCTGAC
//