LOCUS       RC208715                8379 bp    DNA     circular  SYN 
DEFINITION  CASZ1 (Myc-DDK-tagged)-Human castor zinc finger 1 (CASZ1), transcript variant 2
ACCESSION   NM_017766
VERSION     NM_017766.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4536..4627 
                     /label="Myc-DDK"  
     misc_feature    4657..5242 
                     /label="PolyA signal"  
     misc_feature    4714..4733 
                     /label="Restriction Enzyme"  
     misc_feature    5518..6190 
                     /label="ColE1"  
     misc_feature    6620..7414 
                     /label="Kan/Neo"  
     misc_feature    7449..7751 
                     /label="SV40 ori"  
     misc_feature    7837..8314 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4518..4524 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4518 
                     /label="CASZ1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTTGGA ACAGCTGAGG GCACCCGGTG CACGGACCCG CCTGCAGGCA 
     1081 AGCCCGCCAT GGCGCCCAAA CGCAAGGGTG GCCTGAAGCT GAACGCCATC TGCGCCAAGC 
     1141 TGAGCCGCCA GGTGGTGGTG GAGAAGCGAG CTGACGCCGG CTCCCACACG GAGGGCAGCC 
     1201 CATCGCAGCC CCGGGACCAA GAGCGCAGTG GCCCTGAGTC TGGGGCAGCC CGGGCCCCCC 
     1261 GCAGCGAGGA AGACAAGAGA CGGGCAGTGA TCGAGAAGTG GGTGAACGGG GAGTACAGCG 
     1321 AGGAGCCGGC ACCCACACCC GTGTTGGGGC GGATTGCCCG CGAGGGCCTG GAGCTGCCTC 
     1381 CCGAGGGTGT CTACATGGTG CAGCCCCAGG GGTGCAGCGA TGAGGAAGAC CACGCGGAGG 
     1441 AGCCCTCCAA GGACGGCGGT GCCCTGGAGG AGAAGGATTC GGACGGGGCA GCCTCCAAGG 
     1501 AGGACAGCGG CCCCAGCACC AGGCAGGCTT CAGGAGAGGC CTCCTCGCTG CGGGACTACG 
     1561 CGGCCTCCAC CATGACCGAG TTCCTCGGCA TGTTTGGCTA TGATGACCAG AACACGCGGG 
     1621 ACGAGCTGGC CAGGAAGATC AGCTTTGAGA AGCTGCACGC GGGCTCCACC CCGGAGGCAG 
     1681 CCACCTCCTC CATGCTGCCC ACCTCCGAGG ATACCCTCAG CAAGCGGGCG CGGTTCTCTA 
     1741 AGTATGAGGA GTACATCCGC AAGCTCAAGG CTGGCGAGCA GCTCTCCTGG CCGGCCCCCA 
     1801 GCACCAAGAC CGAGGAGCGG GTGGGCAAGG AGGTGGTGGG CACCCTGCCC GGCCTGCGGC 
     1861 TGCCCAGCAG CACGGCCCAC CTGGAGACCA AGGCCACCAT CCTGCCCCTG CCGTCGCACA 
     1921 GCAGTGTCCA GATGCAGAAC CTGGTAGCCC GGGCCTCCAA GTACGACTTC TTCATCCAAA 
     1981 AACTGAAGAC CGGCGAGAAT CTGCGGCCCC AGAACGGGAG CACCTACAAG AAGCCATCCA 
     2041 AGTACGACCT GGAGAATGTC AAGTACCTGC ACCTCTTCAA ACCCGGGGAG GGCAGCCCCG 
     2101 ACATGGGCGG GGCCATCGCC TTCAAGACAG GCAAGGTGGG GCGCCCTTCC AAGTACGACG 
     2161 TCCGGGGCAT CCAGAAGCCA GGCCCCGCCA AGGTTCCGCC CACCCCCAGC CTGGCTCCCG 
     2221 CACCCCTCGC CAGCGTGCCC AGTGCCCCCA GCGCCCCCGG GCCAGGGCCA GAGCCTCCTG 
     2281 CCTCCCTGTC CTTCAACACT CCCGAGTACC TGAAGTCAAC CTTCTCCAAA ACAGACTCCA 
     2341 TCACCACGGG GACCGTCTCC ACTGTCAAGA ACGGACTGCC CACAGATAAA CCAGCCGTCA 
     2401 CTGAAGATGT AAACATTTAC CAGAAATATA TTGCCAGGTT CTCGGGCAGC CAGCACTGTG 
     2461 GCCACATCCA CTGTGCCTAC CAGTACCGCG AGCACTACCA CTGCCTTGAC CCTGAGTGTA 
     2521 ACTACCAGAG GTTCACGAGT AAGCAGGACG TGATCCGCCA CTACAACATG CACAAGAAGC 
     2581 GCGACAACTC CCTGCAGCAC GGCTTCATGC GTTTCAGCCC GCTGGACGAC TGCAGCGTCT 
     2641 ACTACCACGG CTGCCACCTC AATGGGAAGA GCACCCACTA TCACTGCATG CAGGTGGGCT 
     2701 GTAACAAGGT GTACACGAGC ACGTCTGACG TGATGACCCA CGAGAACTTC CACAAGAAGA 
     2761 ATACCCAGCT CATTAACGAC GGCTTCCAGC GCTTCCGAGC CACCGAAGAC TGTGGCACAG 
     2821 CCGACTGCCA GTTCTACGGA CAGAAGACCA CGCACTTCCA CTGCAGGCGC CCCGGCTGCA 
     2881 CATTCACTTT CAAGAACAAG TGTGACATCG AGAAGCACAA GAGCTACCAC ATCAAGGACG 
     2941 ATGCCTACGC CAAGGACGGC TTCAAGAAGT TCTACAAGTA CGAGGAGTGC AAGTACGAGG 
     3001 GCTGCGTGTA CAGCAAGGCT ACCAACCACT TCCACTGCAT CCGCGCCGGC TGCGGCTTCA 
     3061 CCTTCACCTC CACCAGCCAG ATGACCTCTC ACAAGCGCAA GCATGAGCGC CGGCACATCC 
     3121 GCTCCTCGGG CGCGCTGGGG CTGCCGCCCT CGCTGCTGGG CGCCAAGGAC ACGGAGCACG 
     3181 AGGAGTCCAG CAACGACGAC CTTGTTGACT TCTCCGCCCT GAGCAGCAAG AACTCCAGCC 
     3241 TGAGCGCCTC CCCTACCAGC CAGCAGTCCT CTGCGTCCCT GGCTGCCGCC ACTGCCGCCA 
     3301 CCGAGGCTGG GCCCAGTGCC ACCAAACCTC CCAACAGCAA GATCTCGGGG CTGCTGCCCC 
     3361 AGGGCCTGCC TGGCTCGATC CCCCTGGCCC TGGCCCTCTC CAACTCGGGC CTGCCCACCC 
     3421 CCACGCCCTA CTTCCCCATA CTGGCTGGCC GTGGGAGCAC CTCCCCGCCT GTGGGCACCC 
     3481 CCAGCCTCCT GGGTGCCGTG TCGTCTGGGT CAGCAGCCTC AGCCACCCCT GACACACCCA 
     3541 CGCTGGTCGC CTCGGGAGCT GGAGACTCAG CCCCCGTGGC TGCCGCCTCT GTCCCGGCAC 
     3601 CACCCGCCTC CATCATGGAG AGGATCTCTG CAAGCAAGGG CCTCATCTCG CCCATGATGG 
     3661 CCAGGCTGGC TGCAGCTGCC CTTAAGCCCT CTGCCACCTT TGACCCAGGA AGCGGGCAGC 
     3721 AGGTCACCCC AGCCAGGTTC CCCCCGGCCC AAGTGAAGCC GGAACCCGGT GAGAGCACCG 
     3781 GCGCCCCAGG CCCCCACGAA GCCTCCCAGG ACCGCAGTCT AGACCTGACT GTGAAGGAGC 
     3841 CCAGCAACGA ATCAAATGGC CACGCAGTCC CGGCAAATTC ATCTCTTTTA TCCTCGCTTA 
     3901 TGAATAAGAT GTCTCAGGGC AACCCTGGCC TGGGCAGCCT GCTGAACATC AAGGCGGAAG 
     3961 CGGAGGGGAG CCCCGCTGCG GAGCCCTCGC CCTTCCTAGG CAAGGCCGTG AAGGCGCTGG 
     4021 TTCAGGAGAA GTTGGCAGAG CCCTGGAAGG TGTACCTGCG CAGGTTTGGT ACAAAGGACT 
     4081 TCTGTGACGG CCAGTGTGAC TTCCTCCACA AGGCCCACTT CCACTGCGTG GTGGAGGAAT 
     4141 GCGGCGCGCT CTTCAGCACC TTGGACGGGG CCATCAAGCA CGCAAACTTC CACTTCCGGA 
     4201 CAGAGGGAGG AGCAGCAAAA GGAAACACAG AGGCTGCCTT TCCGGCCTCG GCCGCCGAGA 
     4261 CCAAACCTCC CATGGCCCCC TCGTCCCCTC CGGTCCCTCC TGTCACCACG GCCACGGTGT 
     4321 CCTCTCTGGA GGGGCCCGCT CCCAGCCCGG CCTCCGTGCC CTCCACCCCC ACCCTGCTCG 
     4381 CCTGGAAGCA GCTGGCTTCC ACCATACCCC AGATGCCTCA GATCCCAGCG TCAGTGCCTC 
     4441 ACCTGCCCGC CTCGCCCTTG GCAACGACTT CTCTAGAGAA CGCCAAGCCC CAGGTCAAAC 
     4501 CCGGATTCCT CCAGTTCCAG GAGAAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4561 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6001 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6061 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6121 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6181 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6241 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6301 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6361 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6421 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6481 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6541 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6601 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6661 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6721 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6781 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6841 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6901 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6961 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7021 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7081 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7141 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7201 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7261 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7321 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7381 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7441 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7501 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7561 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7621 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7801 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7861 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7921 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7981 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8041 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8101 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8161 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8221 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8281 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8341 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//