LOCUS       RC208705                6585 bp    DNA     circular  SYN 
DEFINITION  SGPL1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate lyase 1 (SGPL1)
ACCESSION   NM_003901
VERSION     NM_003901.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2742..2833 
                     /label="Myc-DDK"  
     misc_feature    2863..3448 
                     /label="PolyA signal"  
     misc_feature    2920..2939 
                     /label="Restriction Enzyme"  
     misc_feature    3724..4396 
                     /label="ColE1"  
     misc_feature    4826..5620 
                     /label="Kan/Neo"  
     misc_feature    5655..5957 
                     /label="SV40 ori"  
     misc_feature    6043..6520 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2724..2730 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2724 
                     /label="SGPL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTAGCACA GACCTTCTGA TGTTGAAGGC CTTTGAGCCC TACTTAGAGA 
     1081 TTTTGGAAGT ATACTCCACA AAAGCCAAGA ATTATGTAAA TGGACATTGC ACCAAGTATG 
     1141 AGCCCTGGCA GCTAATTGCA TGGAGTGTCG TGTGGACCCT GCTGATAGTC TGGGGATATG 
     1201 AGTTTGTCTT CCAGCCAGAG AGTTTATGGT CAAGGTTTAA AAAGAAATGT TTTAAGCTCA 
     1261 CCAGGAAGAT GCCCATTATT GGTCGTAAGA TTCAAGACAA GTTGAACAAG ACCAAGGATG 
     1321 ATATTAGCAA GAACATGTCA TTCCTGAAAG TGGACAAAGA GTATGTGAAA GCTTTACCCT 
     1381 CCCAGGGTCT GAGCTCATCT GCTGTTTTGG AGAAACTTAA GGAGTACAGC TCTATGGACG 
     1441 CCTTCTGGCA AGAGGGGAGA GCCTCTGGAA CAGTGTACAG TGGGGAGGAG AAGCTCACTG 
     1501 AGCTCCTTGT GAAGGCTTAT GGAGATTTTG CATGGAGTAA CCCCCTGCAT CCAGATATCT 
     1561 TCCCAGGACT ACGCAAGATA GAGGCAGAAA TTGTGAGGAT AGCTTGTTCC CTGTTCAATG 
     1621 GGGGACCAGA TTCGTGTGGA TGTGTGACTT CTGGGGGAAC AGAAAGCATA CTGATGGCCT 
     1681 GCAAAGCATA TCGGGATCTG GCCTTTGAGA AGGGGATCAA AACTCCAGAA ATTGTGGCTC 
     1741 CCCAAAGTGC CCATGCTGCA TTTAACAAAG CAGCCAGTTA CTTTGGGATG AAGATTGTGC 
     1801 GGGTCCCATT GACGAAGATG ATGGAGGTGG ATGTGCGGGC AATGAGAAGA GCTATCTCCA 
     1861 GGAACACTGC CATGCTCGTC TGTTCTACCC CACAGTTTCC TCATGGTGTA ATAGATCCTG 
     1921 TCCCTGAAGT GGCCAAGCTG GCTGTCAAAT ACAAAATACC CCTTCATGTC GACGCTTGTC 
     1981 TGGGAGGCTT CCTCATCGTC TTTATGGAGA AAGCAGGATA CCCACTGGAG CACCCATTTG 
     2041 ATTTCCGGGT GAAAGGTGTA ACCAGCATTT CAGCTGACAC CCATAAGTAT GGCTATGCCC 
     2101 CAAAAGGCTC ATCATTGGTG TTGTATAGTG ACAAGAAGTA CAGGAACTAT CAGTTCTTCG 
     2161 TCGATACAGA TTGGCAGGGT GGCATCTATG CTTCCCCAAC CATCGCAGGC TCACGGCCTG 
     2221 GTGGCATTAG CGCAGCCTGT TGGGCTGCCT TGATGCACTT CGGTGAGAAC GGCTATGTTG 
     2281 AAGCTACCAA ACAGATCATC AAAACTGCTC GCTTCCTCAA GTCAGAACTG GAAAATATCA 
     2341 AAGGCATCTT TGTTTTTGGG AATCCCCAAT TGTCAGTCAT TGCTCTGGGA TCCCGTGATT 
     2401 TTGACATCTA CCGACTATCA AACCTGATGA CTGCTAAGGG GTGGAACTTG AACCAGTTGC 
     2461 AGTTCCCACC CAGTATTCAT TTCTGCATCA CATTACTACA CGCCCGGAAA CGAGTAGCTA 
     2521 TACAATTCCT AAAGGACATT CGAGAATCTG TCACTCAAAT CATGAAGAAT CCTAAAGCGA 
     2581 AGACCACAGG AATGGGTGCC ATCTATGGCA TGGCCCAGAC AACTGTTGAC AGGAATATGG 
     2641 TTGCAGAATT GTCCTCAGTC TTCTTGGACA GCTTGTACAG CACCGACACT GTCACCCAGG 
     2701 GCAGCCAGAT GAATGGTTCT CCAAAACCCC ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     2761 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     2821 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     2881 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     2941 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3001 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3061 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3121 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3181 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3241 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3301 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3361 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3421 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3481 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     3541 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     3601 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     3661 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     3721 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     3781 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     3841 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     3901 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     3961 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4021 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4081 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4141 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4201 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4261 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4321 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4381 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4441 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4501 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     4561 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     4621 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     4681 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     4741 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     4801 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     4861 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     4921 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     4981 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5041 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5101 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5161 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5221 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5281 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5341 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5401 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5461 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     5521 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     5581 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     5641 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     5701 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     5761 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     5821 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     5881 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     5941 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6001 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6061 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6121 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6181 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6241 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6301 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6361 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6421 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6481 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     6541 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//