LOCUS       RC208632L2                9465 bp    DNA     circular  SYN 
DEFINITION  LRRC8A (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member A (LRRC8A), transcript variant 2
ACCESSION   NM_019594
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7756..7936 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8664..9323 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7984..8603 
                     /label="ColE1"  
     misc_feature    6631..6651 
                     /label="LR50"  
     misc_feature    5771..6496 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6640..7231 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5750..5756 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5750 
                     /label="LRRC8A"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GATTCCGGTG ACAGAGCTCC GCTACTTTGC 
     3361 GGACACGCAG CCAGCATACC GGATCCTGAA GCCGTGGTGG GATGTGTTCA CAGACTACAT 
     3421 CTCTATCGTC ATGCTGATGA TTGCCGTCTT CGGGGGGACG CTGCAGGTCA CCCAAGACAA 
     3481 GATGATCTGC CTGCCTTGTA AGTGGGTCAC CAAGGACTCC TGCAATGATT CGTTCCGGGG 
     3541 CTGGGCAGCC CCTGGCCCGG AGCCCACCTA CCCCAACTCC ACCATTCTGC CGACCCCTGA 
     3601 CACGGGCCCC ACAGGCATCA AGTATGACCT GGACCGGCAC CAGTACAACT ACGTGGACGC 
     3661 TGTGTGCTAT GAGAACCGAC TGCACTGGTT TGCCAAGTAC TTCCCCTACC TGGTGCTTCT 
     3721 GCACACGCTC ATCTTCCTGG CCTGCAGCAA CTTCTGGTTC AAATTCCCGC GCACCAGCTC 
     3781 GAAGCTGGAG CACTTTGTGT CTATCCTGCT GAAGTGCTTC GACTCGCCCT GGACCACGAG 
     3841 GGCCCTGTCG GAGACAGTGG TGGAGGAGAG CGACCCCAAG CCGGCCTTCA GCAAGATGAA 
     3901 TGGGTCCATG GACAAAAAGT CATCGACCGT CAGTGAGGAC GTGGAGGCCA CCGTGCCCAT 
     3961 GCTGCAGCGG ACCAAGTCAC GGATCGAGCA GGGTATCGTG GACCGCTCAG AGACGGGCGT 
     4021 GCTGGACAAG AAGGAGGGGG AGCAAGCCAA GGCGCTGTTT GAGAAGGTGA AGAAGTTCCG 
     4081 GACCCATGTG GAGGAGGGGG ACATTGTGTA CCGCCTCTAC ATGCGGCAGA CCATCATCAA 
     4141 GGTGATCAAG TTCATCCTCA TCATCTGCTA CACCGTCTAC TACGTGCACA ACATCAAGTT 
     4201 CGACGTGGAC TGCACCGTGG ACATTGAGAG CCTGACGGGC TACCGCACCT ACCGCTGTGC 
     4261 CCACCCCCTG GCCACACTCT TCAAGATCCT GGCGTCCTTC TACATCAGCC TAGTCATCTT 
     4321 CTACGGCCTC ATCTGCATGT ATACACTGTG GTGGATGCTA CGGCGCTCCC TCAAGAAGTA 
     4381 CTCGTTTGAG TCGATCCGTG AGGAGAGCAG CTACAGCGAC ATCCCCGACG TCAAGAACGA 
     4441 CTTCGCCTTC ATGCTGCACC TCATTGACCA ATACGACCCG CTCTACTCCA AGCGCTTCGC 
     4501 CGTCTTCCTG TCGGAGGTGA GTGAGAACAA GCTGCGGCAG CTGAACCTCA ACAACGAGTG 
     4561 GACGCTGGAC AAGCTCCGGC AGCGGCTCAC CAAGAACGCG CAGGACAAGC TGGAGCTGCA 
     4621 CCTGTTCATG CTCAGTGGCA TCCCTGACAC TGTGTTTGAC CTGGTGGAGC TGGAGGTCCT 
     4681 CAAGCTGGAG CTGATCCCCG ACGTGACCAT CCCGCCCAGC ATTGCCCAGC TCACGGGCCT 
     4741 CAAGGAGCTG TGGCTCTACC ACACAGCGGC CAAGATTGAA GCGCCCGCGC TGGCCTTCCT 
     4801 GCGCGAGAAC CTGCGGGCGC TGCACATCAA GTTCACCGAC ATCAAGGAGA TCCCGCTGTG 
     4861 GATCTATAGC CTGAAGACAC TGGAGGAGCT GCACCTGACG GGCAACCTGA GCGCGGAGAA 
     4921 CAACCGCTAC ATCGTCATCG ACGGGCTGCG GGAGCTCAAA CGCCTCAAGG TGCTGCGGCT 
     4981 CAAGAGCAAC CTAAGCAAGC TGCCACAGGT GGTCACAGAT GTGGGCGTGC ACCTGCAGAA 
     5041 GCTGTCCATC AACAATGAGG GCACCAAGCT CATCGTCCTC AACAGCCTCA AGAAGATGGC 
     5101 GAACCTGACT GAGCTGGAGC TGATCCGCTG TGACCTGGAG CGCATCCCCC ACTCCATCTT 
     5161 CAGCCTCCAC AACCTGCAGG AGATTGACCT CAAGGACAAC AACCTCAAGA CCATCGAGGA 
     5221 GATCATCAGC TTCCAGCACC TGCACCGCCT CACCTGCCTT AAGCTGTGGT ACAACCACAT 
     5281 CGCCTACATC CCCATCCAGA TCGGCAACCT CACCAACCTG GAGCGCCTCT ACCTGAACCG 
     5341 CAACAAGATC GAGAAGATCC CCACCCAGCT CTTCTACTGC CGCAAGCTGC GCTACCTGGA 
     5401 CCTCAGCCAC AACAACCTGA CCTTCCTCCC TGCCGACATC GGCCTCCTGC AGAACCTCCA 
     5461 GAACCTAGCC ATCACGGCCA ACCGGATCGA GACGCTCCCT CCGGAGCTCT TCCAGTGCCG 
     5521 GAAGCTGCGG GCCCTGCACC TGGGCAACAA CGTGCTGCAG TCACTGCCCT CCAGGGTGGG 
     5581 CGAGCTGACC AACCTGACGC AGATCGAGCT GCGGGGCAAC CGGCTGGAGT GCCTGCCTGT 
     5641 GGAGCTGGGC GAGTGCCCAC TGCTCAAGCG CAGCGGCTTG GTGGTGGAGG AGGACCTGTT 
     5701 CAACACACTG CCACCCGAGG TGAAGGAGCG GCTGTGGAGG GCTGACAAGG AGCAGGCCAC 
     5761 GCGTACGCGG CCGCTCGAGA TGAGCGGGGG CGAGGAGCTG TTCGCCGGCA TCGTGCCCGT 
     5821 GCTGATCGAG CTGGACGGCG ACGTGCACGG CCACAAGTTC AGCGTGCGCG GCGAGGGCGA 
     5881 GGGCGACGCC GACTACGGCA AGCTGGAGAT CAAGTTCATC TGCACCACCG GCAAGCTGCC 
     5941 CGTGCCCTGG CCCACCCTGG TGACCACCCT CTGCTACGGC ATCCAGTGCT TCGCCCGCTA 
     6001 CCCCGAGCAC ATGAAGATGA ACGACTTCTT CAAGAGCGCC ATGCCCGAGG GCTACATCCA 
     6061 GGAGCGCACC ATCCAGTTCC AGGACGACGG CAAGTACAAG ACCCGCGGCG AGGTGAAGTT 
     6121 CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC AAGGACTTCA AGGAGGACGG 
     6181 CAACATCCTG GGCCACAAGC TGGAGTACAG CTTCAACAGC CACAACGTGT ACATCCGCCC 
     6241 CGACAAGGCC AACAACGGCC TGGAGGCTAA CTTCAAGACC CGCCACAACA TCGAGGGCGG 
     6301 CGGCGTGCAG CTGGCCGACC ACTACCAGAC CAACGTGCCC CTGGGCGACG GCCCCGTGCT 
     6361 GATCCCCATC AACCACTACC TGAGCACTCA GACCAAGATC AGCAAGGACC GCAACGAGGC 
     6421 CCGCGACCAC ATGGTGCTCC TGGAGTCCTT CAGCGCCTGC TGCCACACCC ACGGCATGGA 
     6481 CGAGCTGTAC AGGTCCGGAC TCAGATAAGT TTAAACGGCC GGCCGCGGTC TGTACAAGTA 
     6541 GGATTCGTCG AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT 
     6601 TAAGGGTTCC GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG 
     6661 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 
     6721 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 
     6781 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 
     6841 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 
     6901 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 
     6961 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 
     7021 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 
     7081 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 
     7141 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 
     7201 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG 
     7261 AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG 
     7321 TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC 
     7381 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 
     7441 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA 
     7501 CCACACACAA GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA 
     7561 TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA 
     7621 AGCCAATGAA GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA 
     7681 CCCGGAGAGA GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC 
     7741 CCGAGAGCTG CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 
     7801 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 
     7861 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 
     7921 TTAGTCAGTG TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     7981 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     8041 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     8101 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     8161 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     8221 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     8281 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     8341 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     8401 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     8461 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     8521 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     8581 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     8641 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG 
     8701 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 
     8761 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG 
     8821 AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC 
     8881 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC 
     8941 AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG 
     9001 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA 
     9061 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC 
     9121 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA 
     9181 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA 
     9241 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA 
     9301 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC 
     9361 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     9421 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC
//