LOCUS       RC208606                7941 bp    DNA     circular  SYN 
DEFINITION  ATP1A2 (Myc-DDK-tagged)-Human ATPase, Na+/K+ transporting, alpha 2 polypeptide (ATP1A2)
ACCESSION   NM_000702
VERSION     NM_000702.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4098..4189 
                     /label="Myc-DDK"  
     misc_feature    4219..4804 
                     /label="PolyA signal"  
     misc_feature    4276..4295 
                     /label="Restriction Enzyme"  
     misc_feature    5080..5752 
                     /label="ColE1"  
     misc_feature    6182..6976 
                     /label="Kan/Neo"  
     misc_feature    7011..7313 
                     /label="SV40 ori"  
     misc_feature    7399..7876 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4080..4086 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4080 
                     /label="ATP1A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCGTGGG GCTGGCCGTG AGTACTCACC TGCCGCCACC ACGGCAGAGA 
     1081 ATGGGGGCGG CAAGAAGAAA CAGAAGGAGA AGGAACTGGA TGAGCTGAAG AAGGAGGTGG 
     1141 CAATGGATGA CCACAAGCTG TCCTTGGATG AGCTGGGCCG CAAATACCAA GTGGACCTGT 
     1201 CCAAGGGCCT CACCAACCAG CGGGCTCAGG ACGTTCTGGC TCGAGATGGG CCCAACGCCC 
     1261 TCACACCACC TCCCACAACC CCTGAGTGGG TCAAGTTCTG CCGTCAGCTT TTCGGGGGGT 
     1321 TCTCCATCCT GCTGTGGATT GGGGCTATCC TCTGCTTCCT GGCCTACGGC ATCCAGGCTG 
     1381 CCATGGAGGA TGAACCATCC AACGACAATC TATATCTGGG TGTGGTGCTG GCAGCTGTGG 
     1441 TCATTGTCAC TGGCTGCTTC TCCTACTACC AGGAGGCCAA GAGCTCCAAG ATCATGGATT 
     1501 CCTTCAAGAA CATGGTACCT CAGCAAGCCC TTGTGATCCG GGAGGGAGAG AAGATGCAGA 
     1561 TCAACGCAGA GGAAGTGGTG GTGGGAGACC TGGTGGAGGT GAAGGGTGGA GACCGCGTCC 
     1621 CTGCTGACCT CCGGATCATC TCTTCTCATG GCTGTAAGGT GGATAACTCA TCCTTAACAG 
     1681 GAGAGTCGGA GCCCCAGACC CGCTCCCCCG AGTTCACCCA TGAGAACCCC CTGGAGACCC 
     1741 GCAATATCTG TTTCTTCTCC ACCAACTGTG TTGAAGGCAC TGCCAGGGGC ATTGTGATTG 
     1801 CCACAGGAGA CCGGACGGTG ATGGGCCGCA TAGCTACTCT CGCCTCAGGC CTGGAGGTTG 
     1861 GGCGGACACC CATAGCAATG GAGATTGAAC ACTTCATCCA GCTGATCACA GGGGTCGCTG 
     1921 TATTCCTGGG GGTCTCCTTC TTCGTGCTCT CCCTCATCCT GGGCTACAGC TGGCTGGAGG 
     1981 CAGTCATCTT CCTCATCGGC ATCATAGTGG CCAACGTGCC TGAGGGGCTT CTGGCCACTG 
     2041 TCACTGTGTG CCTGACCCTG ACAGCCAAGC GCATGGCACG GAAGAACTGC CTGGTGAAGA 
     2101 ACCTGGAGGC GGTGGAGACG CTGGGCTCCA CGTCCACCAT CTGCTCGGAC AAGACGGGCA 
     2161 CCCTCACCCA GAACCGCATG ACCGTCGCCC ACATGTGGTT CGACAACCAA ATCCATGAGG 
     2221 CTGACACCAC CGAAGATCAG TCTGGGGCCA CTTTTGACAA ACGATCCCCT ACGTGGACGG 
     2281 CCCTGTCTCG AATTGCTGGT CTCTGCAACC GCGCCGTCTT CAAGGCAGGA CAGGAGAACA 
     2341 TCTCCGTGTC TAAGCGGGAC ACAGCTGGTG ATGCCTCTGA GTCAGCTCTG CTCAAGTGCA 
     2401 TTGAGCTCTC CTGTGGCTCA GTGAGGAAAA TGAGAGACAG AAACCCCAAG GTGGCAGAGA 
     2461 TTCCTTTCAA CTCTACCAAC AAGTACCAGC TGTCTATCCA CGAGCGAGAA GACAGCCCCC 
     2521 AGAGCCACGT GCTGGTGATG AAGGGGGCCC CAGAGCGCAT TCTGGACCGG TGCTCCACCA 
     2581 TCCTGGTGCA GGGCAAGGAG ATCCCGCTCG ACAAGGAGAT GCAAGATGCC TTTCAAAATG 
     2641 CCTACATGGA GCTGGGGGGA CTTGGGGAGC GTGTGCTGGG ATTCTGTCAA CTGAATCTGC 
     2701 CATCTGGAAA GTTTCCTCGG GGCTTCAAAT TCGACACGGA TGAGCTGAAC TTTCCCACGG 
     2761 AGAAGCTTTG CTTTGTGGGG CTCATGTCTA TGATTGACCC TCCCCGGGCT GCTGTGCCAG 
     2821 ATGCTGTGGG CAAGTGCCGA AGCGCAGGCA TCAAGGTGAT CATGGTAACC GGGGATCACC 
     2881 CTATCACAGC CAAGGCCATT GCCAAAGGCG TGGGCATCAT ATCAGAGGGT AACGAGACTG 
     2941 TGGAGGACAT TGCAGCCCGG CTCAACATTC CCATGAGTCA AGTCAACCCC AGAGAAGCCA 
     3001 AGGCATGCGT GGTGCACGGC TCTGACCTGA AGGACATGAC ATCGGAGCAG CTCGATGAGA 
     3061 TCCTCAAGAA CCACACAGAG ATCGTCTTTG CTCGAACGTC TCCCCAGCAG AAGCTCATCA 
     3121 TTGTGGAGGG ATGTCAGAGG CAGGGAGCCA TTGTGGCCGT GACGGGTGAC GGGGTGAACG 
     3181 ACTCCCCTGC ATTGAAGAAG GCTGACATTG GCATTGCCAT GGGCATCTCT GGCTCTGACG 
     3241 TCTCTAAGCA GGCAGCCGAC ATGATCCTGC TGGATGACAA CTTTGCCTCC ATCGTCACGG 
     3301 GGGTGGAGGA GGGCCGCCTG ATCTTTGACA ACTTGAAGAA ATCCATCGCC TACACCCTGA 
     3361 CCAGCAACAT CCCCGAGATC ACCCCCTTCC TGCTGTTCAT CATTGCCAAC ATCCCCCTAC 
     3421 CTCTGGGCAC TGTGACCATC CTTTGCATTG ACCTGGGCAC AGATATGGTC CCTGCCATCT 
     3481 CCTTGGCCTA TGAGGCAGCT GAGAGTGATA TCATGAAGCG GCAGCCACGA AACTCCCAGA 
     3541 CGGACAAGCT GGTGAATGAG AGGCTCATCA GCATGGCCTA CGGACAGATC GGGATGATCC 
     3601 AGGCACTGGG TGGCTTCTTC ACCTACTTTG TGATCCTGGC AGAGAACGGT TTCCTGCCAT 
     3661 CACGGCTACT GGGAATCCGC CTCGACTGGG ATGACCGGAC CATGAATGAT CTGGAGGACA 
     3721 GCTATGGACA GGAGTGGACC TATGAGCAGC GGAAGGTGGT GGAGTTCACG TGCCACACGG 
     3781 CATTCTTTGC CAGCATCGTG GTGGTGCAGT GGGCTGACCT CATCATCTGC AAGACCCGCC 
     3841 GCAACTCAGT CTTCCAGCAG GGCATGAAGA ACAAGATCCT GATTTTTGGG CTCCTGGAGG 
     3901 AGACGGCGTT GGCTGCCTTT CTCTCTTACT GCCCAGGCAT GGGTGTAGCC CTCCGCATGT 
     3961 ACCCGCTCAA AGTCACCTGG TGGTTCTGCG CCTTCCCCTA CAGCCTCCTC ATCTTCATCT 
     4021 ATGATGAGGT CCGAAAGCTC ATCCTGCGGC GGTATCCTGG TGGCTGGGTG GAGAAGGAGA 
     4081 CATACTACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4141 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4201 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4261 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4321 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4381 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4441 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4501 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4561 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4621 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4681 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4741 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4801 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4861 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4921 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4981 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5041 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5101 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5161 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5221 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5281 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5341 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5401 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5461 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5521 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5581 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5641 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5701 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5761 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5821 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5881 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5941 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6001 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6061 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6121 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6181 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6241 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6301 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6361 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6421 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6481 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6541 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6601 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6661 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6721 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6781 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6841 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6901 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6961 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7021 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7081 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7141 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7201 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7261 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7321 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7381 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7441 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7501 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7561 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7621 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7681 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7741 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7801 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7861 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7921 CAAAAATTTA ACGCGAATTT T
//