LOCUS       RC208603                6822 bp    DNA     circular  SYN 
DEFINITION  FZD1 (Myc-DDK-tagged)-Human frizzled family receptor 1 (FZD1)
ACCESSION   NM_003505
VERSION     NM_003505.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2979..3070 
                     /label="Myc-DDK"  
     misc_feature    3100..3685 
                     /label="PolyA signal"  
     misc_feature    3157..3176 
                     /label="Restriction Enzyme"  
     misc_feature    3961..4633 
                     /label="ColE1"  
     misc_feature    5063..5857 
                     /label="Kan/Neo"  
     misc_feature    5892..6194 
                     /label="SV40 ori"  
     misc_feature    6280..6757 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2961..2967 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2961 
                     /label="FZD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGAGGAG GAGGCGCCTA AGAAGTCCCG GGCCGCCGGC GGTGGCGCGA 
     1081 GCTGGGAACT TTGTGCCGGG GCGCTCTCGG CCCGGCTGGC GGAGGAGGGC AGCGGGGACG 
     1141 CCGGTGGCCG CCGCCGCCCG CCAGTTGACC CCCGGCGATT GGCGCGCCAG CTGCTGCTGC 
     1201 TGCTTTGGCT GCTGGAGGCT CCGCTGCTGC TGGGGGTCCG GGCCCAGGCG GCGGGCCAGG 
     1261 GGCCAGGCCA GGGGCCCGGG CCGGGGCAGC AACCGCCGCC GCCGCCTCAG CAGCAACAGA 
     1321 GCGGGCAGCA GTACAACGGC GAGCGGGGCA TCTCCGTCCC GGACCACGGC TATTGCCAGC 
     1381 CCATCTCCAT CCCGCTGTGC ACGGACATCG CGTACAACCA GACCATCATG CCCAACCTGC 
     1441 TGGGCCACAC GAACCAGGAG GACGCGGGCC TGGAGGTGCA CCAGTTCTAC CCTCTAGTGA 
     1501 AAGTGCAGTG TTCCGCTGAG CTCAAGTTCT TCCTGTGCTC CATGTACGCG CCCGTGTGCA 
     1561 CCGTGCTAGA GCAGGCGCTG CCGCCCTGCC GCTCCCTGTG CGAGCGCGCG CGCCAGGGCT 
     1621 GCGAGGCGCT CATGAACAAG TTCGGCTTCC AGTGGCCAGA CACGCTCAAG TGTGAGAAGT 
     1681 TCCCGGTGCA CGGCGCCGGC GAGCTGTGCG TGGGCCAGAA CACGTCCGAC AAGGGCACCC 
     1741 CGACGCCCTC GCTGCTTCCA GAGTTCTGGA CCAGCAACCC TCAGCACGGC GGCGGAGGGC 
     1801 ACCGTGGCGG CTTCCCGGGG GGCGCCGGCG CGTCGGAGCG AGGCAAGTTC TCCTGCCCGC 
     1861 GCGCCCTCAA GGTGCCCTCC TACCTCAACT ACCACTTCCT GGGGGAGAAG GACTGCGGCG 
     1921 CACCTTGTGA GCCGACCAAG GTGTATGGGC TCATGTACTT CGGGCCCGAG GAGCTGCGCT 
     1981 TCTCGCGCAC CTGGATTGGC ATTTGGTCAG TGCTGTGCTG CGCCTCCACG CTCTTCACGG 
     2041 TGCTTACGTA CCTGGTGGAC ATGCGGCGCT TCAGCTACCC GGAGCGGCCC ATCATCTTCT 
     2101 TGTCCGGCTG TTACACGGCC GTGGCCGTGG CCTACATCGC CGGCTTCCTC CTGGAAGACC 
     2161 GAGTGGTGTG TAATGACAAG TTCGCCGAGG ACGGGGCACG CACTGTGGCG CAGGGCACCA 
     2221 AGAAGGAGGG CTGCACCATC CTCTTCATGA TGCTCTACTT CTTCAGCATG GCCAGCTCCA 
     2281 TCTGGTGGGT GATCCTGTCG CTCACCTGGT TCCTGGCGGC TGGCATGAAG TGGGGCCACG 
     2341 AGGCCATCGA AGCCAACTCA CAGTATTTTC ACCTGGCCGC CTGGGCTGTG CCGGCCATCA 
     2401 AGACCATCAC CATCCTGGCG CTGGGCCAGG TGGACGGCGA TGTGCTGAGC GGAGTGTGCT 
     2461 TCGTGGGGCT TAACAACGTG GACGCGCTGC GTGGCTTCGT GCTGGCGCCC CTCTTCGTGT 
     2521 ACCTGTTTAT CGGCACGTCC TTTCTGCTGG CCGGCTTTGT GTCGCTCTTC CGCATCCGCA 
     2581 CCATCATGAA GCACGATGGC ACCAAGACCG AGAAGCTGGA GAAGCTCATG GTGCGCATTG 
     2641 GCGTCTTCAG CGTGCTGTAC ACTGTGCCAG CCACCATCGT CATCGCCTGC TACTTCTACG 
     2701 AGCAGGCCTT CCGGGACCAG TGGGAACGCA GCTGGGTGGC CCAGAGCTGC AAGAGCTACG 
     2761 CTATCCCCTG CCCTCACCTC CAGGCGGGCG GAGGCGCCCC GCCGCACCCG CCCATGAGCC 
     2821 CGGACTTCAC GGTCTTCATG ATTAAGTACC TTATGACGCT GATCGTGGGC ATCACGTCGG 
     2881 GCTTCTGGAT CTGGTCCGGC AAGACCCTCA ACTCCTGGAG GAAGTTCTAC ACGAGGCTCA 
     2941 CCAACAGCAA ACAAGGGGAG ACTACAGTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3001 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3061 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3121 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3181 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3241 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3301 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3361 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3421 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3481 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3541 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3601 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3661 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3721 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3781 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3841 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3901 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3961 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4021 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4081 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4141 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4201 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4261 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4321 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4381 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4441 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4501 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4561 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4621 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4681 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4741 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4801 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4861 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4921 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4981 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5041 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5101 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5161 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5221 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5281 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5341 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5401 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5461 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5521 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5581 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5641 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5701 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5761 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5821 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5881 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5941 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6001 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6061 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6121 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6181 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6241 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6301 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6361 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6421 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6481 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6541 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6601 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6661 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6721 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6781 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//