LOCUS       RC208562                7965 bp    DNA     circular  SYN 
DEFINITION  COL6A1 (Myc-DDK-tagged)-Human collagen, type VI, alpha 1 (COL6A1)
ACCESSION   NM_001848
VERSION     NM_001848.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4122..4213 
                     /label="Myc-DDK"  
     misc_feature    4243..4828 
                     /label="PolyA signal"  
     misc_feature    4300..4319 
                     /label="Restriction Enzyme"  
     misc_feature    5104..5776 
                     /label="ColE1"  
     misc_feature    6206..7000 
                     /label="Kan/Neo"  
     misc_feature    7035..7337 
                     /label="SV40 ori"  
     misc_feature    7423..7900 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4104..4110 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4104 
                     /label="COL6A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGCGGCC CGTGCTCTGC TGCCCCTGCT GCTGCAGGCC TGCTGGACAG 
     1081 CCGCGCAGGA TGAGCCGGAG ACCCCGAGGG CCGTGGCCTT CCAGGACTGC CCCGTGGACC 
     1141 TGTTCTTTGT GCTGGACACC TCTGAGAGCG TGGCCCTGAG GCTGAAGCCC TACGGGGCCC 
     1201 TCGTGGACAA AGTCAAGTCC TTCACCAAGC GCTTCATCGA CAACCTGAGG GACAGGTACT 
     1261 ACCGCTGTGA CCGAAACCTG GTGTGGAACG CAGGCGCGCT GCACTACAGT GACGAGGTGG 
     1321 AGATCATCCA AGGCCTCACG CGCATGCCTG GCGGCCGCGA CGCACTCAAA AGCAGCGTGG 
     1381 ACGCGGTCAA GTACTTTGGG AAGGGCACCT ACACCGACTG CGCTATCAAG AAGGGGCTGG 
     1441 AGCAGCTCCT CGTGGGGGGC TCCCACCTGA AGGAGAATAA GTACCTGATT GTGGTGACCG 
     1501 ACGGGCACCC CCTGGAGGGC TACAAGGAAC CCTGTGGGGG GCTGGAGGAT GCTGTGAACG 
     1561 AGGCCAAGCA CCTGGGCGTC AAAGTCTTCT CGGTGGCCAT CACACCCGAC CACCTGGAGC 
     1621 CGCGTCTGAG CATCATCGCC ACGGACCACA CGTACCGGCG CAACTTCACG GCGGCTGACT 
     1681 GGGGCCAGAG CCGCGACGCA GAGGAGGCCA TCAGCCAGAC CATCGACACC ATCGTGGACA 
     1741 TGATCAAAAA TAACGTGGAG CAAGTGTGCT GCTCCTTCGA ATGCCAGCCT GCAAGAGGAC 
     1801 CTCCGGGGCT CCGGGGCGAC CCCGGCTTTG AGGGAGAACG AGGCAAGCCG GGGCTCCCAG 
     1861 GAGAGAAGGG AGAAGCCGGA GATCCTGGAA GACCCGGGGA CCTCGGACCT GTTGGGTACC 
     1921 AGGGAATGAA GGGAGAAAAA GGGAGCCGTG GGGAGAAGGG CTCCAGGGGA CCCAAGGGCT 
     1981 ACAAGGGAGA GAAGGGCAAG CGTGGCATCG ACGGGGTGGA CGGCGTGAAG GGGGAGATGG 
     2041 GGTACCCAGG CCTGCCAGGC TGCAAGGGCT CGCCCGGGTT TGACGGCATT CAAGGACCCC 
     2101 CTGGCCCCAA GGGAGACCCC GGCGCCTTTG GACTGAAAGG AGAAAAGGGC GAGCCTGGAG 
     2161 CTGACGGGGA GGCGGGGAGA CCAGGGAGCT CGGGACCATC TGGAGACGAG GGCCAGCCGG 
     2221 GAGAGCCTGG GCCCCCCGGA GAGAAAGGAG AGGCGGGCGA CGAGGGGAAC CCAGGACCTG 
     2281 ACGGTGCCCC CGGGGAGCGG GGTGGCCCTG GAGAGAGAGG ACCACGGGGG ACCCCAGGCA 
     2341 CACGGGGACC AAGAGGAGAC CCTGGTGAAG CTGGCCCGCA GGGTGATCAG GGAAGAGAAG 
     2401 GCCCCGTTGG TGTCCCTGGA GACCCGGGCG AGGCTGGCCC TATCGGACCT AAAGGCTACC 
     2461 GAGGCGATGA GGGTCCCCCA GGGTCCGAGG GTGCCAGAGG AGCCCCAGGA CCTGCCGGAC 
     2521 CCCCTGGAGA CCCGGGGCTG ATGGGTGAAA GGGGAGAAGA CGGCCCCGCT GGAAATGGCA 
     2581 CCGAGGGCTT CCCCGGCTTC CCCGGGTATC CGGGCAACAG GGGCGCTCCC GGGATAAACG 
     2641 GCACGAAGGG CTACCCCGGC CTCAAGGGGG ACGAGGGAGA AGCCGGGGAC CCCGGAGACG 
     2701 ATAACAACGA CATTGCACCC CGAGGAGTCA AAGGAGCAAA GGGGTACCGG GGTCCCGAGG 
     2761 GCCCCCAGGG ACCCCCAGGA CACCAAGGAC CGCCTGGGCC GGACGAATGC GAGATTTTGG 
     2821 ACATCATCAT GAAAATGTGC TCTTGCTGTG AATGCAAGTG CGGCCCCATC GACCTCCTGT 
     2881 TCGTGCTGGA CAGCTCAGAG AGCATTGGCC TGCAGAACTT CGAGATTGCC AAGGACTTCG 
     2941 TCGTCAAGGT CATCGACCGG CTGAGCCGGG ACGAGCTGGT CAAGTTCGAG CCAGGGCAGT 
     3001 CGTACGCGGG TGTGGTGCAG TACAGCCACA GCCAGATGCA GGAGCACGTG AGCCTGCGCA 
     3061 GCCCCAGCAT CCGGAACGTG CAGGAGCTCA AGGAAGCCAT CAAGAGCCTG CAGTGGATGG 
     3121 CGGGCGGCAC CTTCACGGGG GAGGCCCTGC AGTACACGCG GGACCAGCTG CTGCCGCCCA 
     3181 GCCCGAACAA CCGCATCGCC CTGGTCATCA CTGACGGGCG CTCAGACACT CAGAGGGACA 
     3241 CCACACCGCT CAACGTGCTC TGCAGCCCCG GCATCCAGGT GGTCTCCGTG GGCATCAAAG 
     3301 ACGTGTTTGA CTTCATCCCA GGCTCAGACC AGCTCAATGT CATTTCTTGC CAAGGCCTGG 
     3361 CACCATCCCA GGGCCGGCCC GGCCTCTCGC TGGTCAAGGA GAACTATGCA GAGCTGCTGG 
     3421 AGGATGCCTT CCTGAAGAAT GTCACCGCCC AGATCTGCAT AGACAAGAAG TGTCCAGATT 
     3481 ACACCTGCCC CATCACGTTC TCCTCCCCGG CTGACATCAC CATCCTGCTG GACGGCTCCG 
     3541 CCAGCGTGGG CAGCCACAAC TTTGACACCA CCAAGCGCTT CGCCAAGCGC CTGGCCGAGC 
     3601 GCTTCCTCAC AGCGGGCAGG ACGGACCCCG CCCACGACGT GCGGGTGGCG GTGGTGCAGT 
     3661 ACAGCGGCAC GGGCCAGCAG CGCCCAGAGC GGGCGTCGCT GCAGTTCCTG CAGAACTACA 
     3721 CGGCCCTGGC CAGTGCCGTC GATGCCATGG ACTTTATCAA CGACGCCACC GACGTCAACG 
     3781 ATGCCCTGGG CTATGTGACC CGCTTCTACC GCGAGGCCTC GTCCGGCGCT GCCAAGAAGA 
     3841 GGCTGCTGCT CTTCTCAGAT GGCAACTCGC AGGGCGCCAC GCCCGCTGCC ATCGAGAAGG 
     3901 CCGTGCAGGA AGCCCAGCGG GCAGGCATCG AGATCTTCGT GGTGGTCGTG GGCCGCCAGG 
     3961 TGAATGAGCC CCACATCCGC GTCCTGGTCA CCGGCAAGAC GGCCGAGTAC GACGTGGCCT 
     4021 ACGGCGAGAG CCACCTGTTC CGTGTCCCCA GCTACCAGGC CCTGCTCCGC GGTGTCTTCC 
     4081 ACCAGACAGT CTCCAGGAAG GTGGCGCTGG GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4141 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4201 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4261 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4321 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4381 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4441 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4501 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4561 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4621 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4681 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4741 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4801 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4861 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4921 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4981 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5041 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5101 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5161 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5221 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5281 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5341 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5401 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5461 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5521 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5581 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5641 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5701 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5761 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5821 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5881 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5941 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6001 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6061 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6121 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6181 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6241 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6301 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6361 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6421 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6481 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6541 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6601 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6661 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6721 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6781 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6841 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6901 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6961 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7021 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7081 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7141 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7201 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7261 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7321 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7381 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7441 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7501 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7561 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7621 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7681 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7741 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7801 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7861 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7921 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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