LOCUS       RC208559L1                9336 bp    DNA     circular  SYN 
DEFINITION  EPHB4 (Myc-DDK-tagged)-Human EPH receptor B4 (EPHB4)
ACCESSION   NM_004444
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7627..7807 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8535..9194 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7966..8534 
                     /label="ColE1"  
     misc_feature    6502..6522 
                     /label="LR50 primer"  
     misc_feature    6299..6370 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6511..7102 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6281..6287 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6281 
                     /label="EPHB4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGCTCCGG GTGCTGCTCT GCTGGGCTTC 
     3361 GTTGGCCGCA GCTTTGGAAG AGACCCTGCT GAACACAAAA TTGGAAACTG CTGATCTGAA 
     3421 GTGGGTGACA TTCCCTCAGG TGGACGGGCA GTGGGAGGAA CTGAGCGGCC TGGATGAGGA 
     3481 ACAGCACAGC GTGCGCACCT ACGAAGTGTG TGACGTGCAG CGTGCCCCGG GCCAGGCCCA 
     3541 CTGGCTTCGC ACAGGTTGGG TCCCACGGCG GGGCGCCGTC CACGTGTACG CCACGCTGCG 
     3601 CTTCACCATG CTCGAGTGCC TGTCCCTGCC TCGGGCTGGG CGCTCCTGCA AGGAGACCTT 
     3661 CACCGTCTTC TACTATGAGA GCGATGCGGA CACGGCCACG GCCCTCACGC CAGCCTGGAT 
     3721 GGAGAACCCC TACATCAAGG TGGACACGGT GGCCGCGGAG CATCTCACCC GGAAGCGCCC 
     3781 TGGGGCCGAG GCCACCGGGA AGGTGAATGT CAAGACGCTG CGTCTGGGAC CGCTCAGCAA 
     3841 GGCTGGCTTC TACCTGGCCT TCCAGGACCA GGGTGCCTGC ATGGCCCTGC TATCCCTGCA 
     3901 CCTCTTCTAC AAAAAGTGCG CCCAGCTGAC TGTGAACCTG ACTCGATTCC CGGAGACTGT 
     3961 GCCTCGGGAG CTGGTTGTGC CCGTGGCCGG TAGCTGCGTG GTGGATGCCG TCCCCGCCCC 
     4021 TGGCCCCAGC CCCAGCCTCT ACTGCCGTGA GGATGGCCAG TGGGCCGAAC AGCCGGTCAC 
     4081 GGGCTGCAGC TGTGCTCCGG GGTTCGAGGC AGCTGAGGGG AACACCAAGT GCCGAGCCTG 
     4141 TGCCCAGGGC ACCTTCAAGC CCCTGTCAGG AGAAGGGTCC TGCCAGCCAT GCCCAGCCAA 
     4201 TAGCCACTCT AACACCATTG GATCAGCCGT CTGCCAGTGC CGCGTCGGGT ACTTCCGGGC 
     4261 ACGCACAGAC CCCCGGGGTG CACCCTGCAC CACCCCTCCT TCGGCTCCGC GGAGCGTGGT 
     4321 TTCCCGCCTG AACGGCTCCT CCCTGCACCT GGAATGGAGT GCCCCCCTGG AGTCTGGTGG 
     4381 CCGAGAGGAC CTCACCTACG CCCTCCGCTG CCGGGAGTGC CGACCCGGAG GCTCCTGTGC 
     4441 GCCCTGCGGG GGAGACCTGA CTTTTGACCC CGGCCCCCGG GACCTGGTGG AGCCCTGGGT 
     4501 GGTGGTTCGA GGGCTACGTC CTGACTTCAC CTATACCTTT GAGGTCACTG CATTGAACGG 
     4561 GGTATCCTCC TTAGCCACGG GGCCCGTCCC ATTTGAGCCT GTCAATGTCA CCACTGACCG 
     4621 AGAGGTACCT CCTGCAGTGT CTGACATCCG GGTGACGCGG TCCTCACCCA GCAGCTTGAG 
     4681 CCTGGCCTGG GCTGTTCCCC GGGCACCCAG TGGGGCTGTG CTGGACTACG AGGTCAAATA 
     4741 CCATGAGAAG GGCGCCGAGG GTCCCAGCAG CGTGCGGTTC CTGAAGACGT CAGAAAACCG 
     4801 GGCAGAGCTG CGGGGGCTGA AGCGGGGAGC CAGCTACCTG GTGCAGGTAC GGGCGCGCTC 
     4861 TGAGGCCGGC TACGGGCCCT TCGGCCAGGA ACATCACAGC CAGACCCAAC TGGATGAGAG 
     4921 CGAGGGCTGG CGGGAGCAGC TGGCCCTGAT TGCGGGCACG GCAGTCGTGG GTGTGGTCCT 
     4981 GGTCCTGGTG GTCATTGTGG TCGCAGTTCT CTGCCTCAGG AAGCAGAGCA ATGGGAGAGA 
     5041 AGCAGAATAT TCGGACAAAC ACGGACAGTA TCTCATCGGA CATGGTACTA AGGTCTACAT 
     5101 CGACCCCTTC ACTTATGAAG ACCCTAATGA GGCTGTGAGG GAATTTGCAA AAGAGATCGA 
     5161 TGTCTCCTAC GTCAAGATTG AAGAGGTGAT TGGTGCAGGT GAGTTTGGCG AGGTGTGTCG 
     5221 GGGGCGGCTC AAGGCCCCAG GGAAGAAGGA GAGCTGTGTG GCAATCAAGA CCCTGAAGGG 
     5281 TGGCTACACG GAGCGGCAGC GGCGTGAGTT TCTGAGCGAG GCCTCCATCA TGGGCCAGTT 
     5341 CGAGCACCCC AATATCATCC GCCTGGAGGG CGTGGTCACC AACAGCATGC CCGTCATGAT 
     5401 TCTCACAGAG TTCATGGAGA ACGGCGCCCT GGACTCCTTC CTGCGGCTAA ACGACGGACA 
     5461 GTTCACAGTC ATCCAGCTCG TGGGCATGCT GCGGGGCATC GCCTCGGGCA TGCGGTACCT 
     5521 TGCCGAGATG AGCTACGTCC ACCGAGACCT GGCTGCTCGC AACATCCTAG TCAACAGCAA 
     5581 CCTCGTCTGC AAAGTGTCTG ACTTTGGCCT TTCCCGATTC CTGGAGGAGA ACTCTTCCGA 
     5641 TCCCACCTAC ACGAGCTCCC TGGGAGGAAA GATTCCCATC CGATGGACTG CCCCGGAGGC 
     5701 CATTGCCTTC CGGAAGTTCA CTTCCGCCAG TGATGCCTGG AGTTACGGGA TTGTGATGTG 
     5761 GGAGGTGATG TCATTTGGGG AGAGGCCGTA CTGGGACATG AGCAATCAGG ACGTGATCAA 
     5821 TGCCATTGAA CAGGACTACC GGCTGCCCCC GCCCCCAGAC TGTCCCACCT CCCTCCACCA 
     5881 GCTCATGCTG GACTGTTGGC AGAAAGACCG GAATGCCCGG CCCCGCTTCC CCCAGGTGGT 
     5941 CAGCGCCCTG GACAAGATGA TCCGGAACCC CGCCAGCCTC AAAATCGTGG CCCGGGAGAA 
     6001 TGGCGGGGCC TCACACCCTC TCCTGGACCA GCGGCAGCCT CACTACTCAG CTTTTGGCTC 
     6061 TGTGGGCGAG TGGCTTCGGG CCATCAAAAT GGGAAGATAC GAAGAAAGTT TCGCAGCCGC 
     6121 TGGCTTTGGC TCCTTCGAGC TGGTCAGCCA GATCTCTGCT GAGGACCTGC TCCGAATCGG 
     6181 AGTCACTCTG GCGGGACACC AGAAGAAAAT CTTGGCCAGT GTCCAGCACA TGAAGTCCCA 
     6241 GGCCAAGCCG GGAACCCCGG GTGGGACAGG AGGACCGGCC CCGCAGTACA CGCGTACGCG 
     6301 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     6361 CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC 
     6421 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC 
     6481 CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA 
     6541 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 
     6601 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 
     6661 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 
     6721 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 
     6781 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 
     6841 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 
     6901 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 
     6961 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 
     7021 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 
     7081 CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA 
     7141 AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT 
     7201 AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC 
     7261 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA 
     7321 AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 
     7381 AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC 
     7441 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA 
     7501 AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 
     7561 AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT 
     7621 GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG 
     7681 CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT 
     7741 GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT 
     7801 GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     7861 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     7921 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     7981 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     8041 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     8101 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     8161 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     8221 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     8281 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     8341 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     8401 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     8461 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     8521 ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 
     8581 AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC 
     8641 GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG 
     8701 GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA 
     8761 TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA 
     8821 CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT 
     8881 TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA 
     8941 ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA 
     9001 TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT 
     9061 ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA 
     9121 ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA 
     9181 TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     9241 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9301 GTCCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//