LOCUS       RC208549                7353 bp    DNA     circular  SYN 
DEFINITION  NPAS2 (Myc-DDK-tagged)-Human neuronal PAS domain protein 2 (NPAS2)
ACCESSION   NM_002518
VERSION     NM_002518.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3510..3601 
                     /label="Myc-DDK"  
     misc_feature    3631..4216 
                     /label="PolyA signal"  
     misc_feature    3688..3707 
                     /label="Restriction Enzyme"  
     misc_feature    4492..5164 
                     /label="ColE1"  
     misc_feature    5594..6388 
                     /label="Kan/Neo"  
     misc_feature    6423..6725 
                     /label="SV40 ori"  
     misc_feature    6811..7288 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3492..3498 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3492 
                     /label="NPAS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAAGAT GAGAAAGACA GAGCCAAGAG AGCTTCTCGA AACAAGTCTG 
     1081 AGAAGAAGCG TCGGGACCAG TTCAATGTTC TCATCAAAGA GCTCAGTTCC ATGCTCCCTG 
     1141 GCAACACGCG GAAAATGGAC AAAACCACCG TGTTGGAAAA GGTCATCGGA TTTTTGCAGA 
     1201 AACACAATGA AGTCTCAGCG CAAACGGAAA TCTGTGACAT TCAGCAAGAC TGGAAGCCTT 
     1261 CATTCCTCAG TAATGAAGAA TTCACCCAGC TGATGTTGGA GGCATTAGAT GGCTTCATTA 
     1321 TCGCAGTGAC AACAGACGGC AGCATCATCT ATGTCTCTGA CAGTATCACG CCTCTCCTTG 
     1381 GGCATTTACC GTCGGATGTC ATGGATCAGA ATTTGTTAAA TTTCCTCCCA GAACAAGAAC 
     1441 ATTCAGAAGT TTATAAAATC CTTTCTTCCC ATATGCTTGT GACGGATTCC CCCTCCCCAG 
     1501 AATACTTAAA ATCTGACAGC GATTTAGAGT TTTATTGCCA TCTTCTCAGA GGCAGCTTGA 
     1561 ACCCAAAGGA ATTTCCAACT TATGAATACA TAAAATTTGT AGGAAATTTT CGCTCTTACA 
     1621 ACAATGTGCC TAGCCCCTCC TGTAATGGTT TTGACAACAC CCTTTCAAGA CCTTGCCGGG 
     1681 TGCCACTAGG AAAGGAGGTT TGCTTCATTG CCACCGTTCG TCTGGCAACA CCACAATTCT 
     1741 TAAAGGAAAT GTGCATAGTT GACGAACCTT TAGAGGAATT CACTTCAAGG CATAGCTTGG 
     1801 AATGGAAATT TTTATTTCTG GATCACAGAG CACCTCCAAT CATAGGATAC CTGCCTTTTG 
     1861 AAGTGCTGGG AACCTCAGGC TATGACTACT ACCACATTGA TGACCTGGAG CTCCTGGCCA 
     1921 GGTGTCACCA GCACCTGATG CAGTTTGGCA AAGGGAAGTC GTGTTGCTAC CGGTTTCTGA 
     1981 CCAAAGGTCA GCAGTGGATC TGGCTGCAGA CTCACTACTA CATCACCTAC CATCAGTGGA 
     2041 ACTCCAAGCC CGAGTTCATC GTGTGCACAC ACTCGGTGGT CAGTTACGCA GATGTCCGGG 
     2101 TGGAAAGGAG GCAGGAGCTG GCTCTGGAAG ACCCGCCATC CGAGGCCCTC CACTCCTCAG 
     2161 CACTAAAGGA CAAGGGCTCA AGCCTGGAAC CTCGGCAGCA CTTTAACACA CTCGACGTGG 
     2221 GTGCCTCGGG CCTTAATACC AGTCATTCGC CATCGGCGTC CTCAAGAAGT TCCCACAAAT 
     2281 CCTCGCACAC AGCCATGTCA GAACCCACCT CCACTCCCAC CAAGCTGATG GCAGAGGCCA 
     2341 GCACCCCGGC TTTGCCAAGA TCAGCCACCC TGCCCCAAGA GTTACCTGTC CCCGGGCTCA 
     2401 GCCAGGCAGC CACCATGCCG GCCCCTCTGC CTTCCCCATC GTCCTGCGAC CTCACACAGC 
     2461 AGCTCCTGCC TCAGACCGTT CTGCAGAGCA CGCCCGCTCC CATGGCACAG TTTTCGGCAC 
     2521 AGTTCAGCAT GTTCCAGACC ATCAAAGACC AGCTAGAGCA GCGGACGCGG ATCCTGCAGG 
     2581 CCAATATCCG GTGGCAACAG GAAGAGCTCC ACAAGATCCA GGAGCAGCTC TGCCTGGTCC 
     2641 AGGACTCCAA CGTCCAGATG TTCCTGCAGC AGCCAGCTGT ATCCCTGAGC TTCAGCAGCA 
     2701 CCCAGCGACC TGAGGCTCAG CAGCAGCTAC AGCAAAGGTC AGCTGCAGTG ACTCAGCCCC 
     2761 AGCTCGGGGC GGGCCCCCAA CTTCCAGGGC AGATCTCCTC TGCCCAGGTC ACAAGCCAGC 
     2821 ACCTGCTCAG AGAATCAAGT GTGATATCAA CCCAGGGTCC AAAGCCAATG AGAAGCTCAC 
     2881 AGCTAATGCA GAGCAGCGGC CGCTCTGGAA GCAGCCTAGT GTCCCCGTTC AGCAGCGCCA 
     2941 CAGCTGCGCT CCCGCCAAGT CTGAATCTGA CCACACCTGC TTCCACCTCC CAGGATGCCA 
     3001 GCCAGTGCCA GCCCAGCCCA GACTTCAGCC ATGATCGGCA GCTCAGGCTG TTGCTGAGCC 
     3061 AGCCCATCCA GCCCATGATG CCCGGGTCCT GTGACGCAAG GCAGCCCTCG GAAGTCAGCA 
     3121 GGACGGGACG GCAAGTCAAG TACGCCCAGA GCCAGACCGT GTTTCAAAAT CCAGACGCAC 
     3181 ACCCCGCCAA CAGCAGCAGC GCCCCGATGC CCGTCCTGCT GATGGGGCAG GCGGTGCTCC 
     3241 ACCCCAGCTT CCCTGCCTCC CAACCATCGC CCCTGCAGCC TGCACAGGCC CGGCAGCAGC 
     3301 CACCGCAGCA CTACCTGCAG GTACAGGCAC CAACCTCTTT GCACAGTGAG CAGCAGGACT 
     3361 CGCTACTTCT CTCCACCTAC TCACAACAGC CAGGGACCCT GGGCTACCCC CAACCACCCC 
     3421 CAGCACAGCC CCAGCCCCTA CGTCCTCCCC GAAGGGTCAG CAGTCTGTCT GAGTCGTCAG 
     3481 GCCTCCAGCA GCCGCCCCGA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3541 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3601 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3661 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3721 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3781 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3841 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3901 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3961 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4021 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4081 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4141 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4201 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4261 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4321 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4381 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4441 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4501 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4561 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4621 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4681 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4741 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4801 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4861 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4921 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4981 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5041 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5101 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5161 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5221 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5281 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5341 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5401 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5461 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5521 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5581 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5641 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5701 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5761 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5821 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5881 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5941 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6001 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6061 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6121 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6181 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6241 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6301 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6361 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6421 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6481 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6541 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6601 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6661 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6721 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6781 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6841 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6901 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6961 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7021 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7081 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7141 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7201 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7261 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7321 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//