LOCUS       RC208531L1                9057 bp    DNA     circular  SYN 
DEFINITION  ACTN2 (Myc-DDK-tagged)-Human actinin, alpha 2 (ACTN2)
ACCESSION   NM_001103
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7348..7528 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8256..8915 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7687..8255 
                     /label="ColE1"  
     misc_feature    6223..6243 
                     /label="LR50 primer"  
     misc_feature    6020..6091 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6232..6823 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6002..6008 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6002 
                     /label="ACTN2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAACCAGATA GAGCCCGGCG TGCAGTACAA 
     3361 CTACGTGTAC GACGAGGATG AGTACATGAT CCAGGAGGAG GAGTGGGACC GCGACCTGCT 
     3421 CCTGGACCCA GCCTGGGAGA AGCAGCAGAG GAAGACCTTC ACTGCCTGGT GTAACTCCCA 
     3481 CCTAAGGAAA GCCGGCACCC AGATTGAGAA CATCGAGGAA GACTTCAGGA ATGGCCTTAA 
     3541 GCTCATGCTG CTTTTGGAAG TCATCTCAGG GGAAAGGCTG CCCAAACCTG ACCGGGGAAA 
     3601 AATGCGGTTC CACAAAATTG CTAATGTCAA CAAAGCTTTG GATTACATAG CCAGCAAAGG 
     3661 GGTGAAACTG GTGTCCATCG GCGCTGAAGA AATTGTTGAT GGCAATGTGA AAATGACCCT 
     3721 GGGTATGATC TGGACCATCA TCCTTCGCTT TGCTATTCAG GATATTTCGG TTGAAGAAAC 
     3781 ATCTGCCAAA GAAGGTCTGC TGCTTTGGTG TCAGAGGAAA ACTGCTCCTT ATAGAAATGT 
     3841 GAACATTCAG AACTTCCATA CTAGCTGGAA AGATGGCCTT GGACTCTGTG CCCTCATCCA 
     3901 CCGACACCGG CCTGACCTCA TTGACTACTC AAAGCTTAAC AAGGATGACC CCATAGGAAA 
     3961 TATTAACCTG GCCATGGAAA TCGCTGAGAA GCACCTGGAT ATTCCTAAAA TGTTGGATGC 
     4021 TGAAGACATC GTGAACACCC CTAAACCCGA TGAAAGAGCC ATCATGACGT ACGTCTCTTG 
     4081 CTTCTACCAC GCTTTTGCGG GCGCGGAGCA GGCCGAGACA GCGGCTAACA GGATATGTAA 
     4141 GGTTCTTGCT GTGAATCAAG AGAATGAGAG GCTGATGGAA GAATATGAGA GGCTAGCGAG 
     4201 TGAGCTTTTG GAATGGATTC GTCGCACGAT CCCCTGGCTG GAGAACCGGA CTCCCGAGAA 
     4261 GACCATGCAA GCCATGCAGA AGAAGCTGGA GGACTTCCGG GATTACCGCC GGAAGCACAA 
     4321 GCCACCCAAG GTGCAGGAGA AATGCCAGCT GGAGATCAAC TTCAACACGC TGCAGACCAA 
     4381 GCTGCGGATC AGCAACCGTC CTGCCTTCAT GCCCTCCGAG GGCAAGATGG TGTCGGATAT 
     4441 TGCTGGTGCC TGGCAGAGGC TGGAGCAGGC TGAGAAGGGT TACGAGGAGT GGTTGCTCAA 
     4501 TGAGATTCGG AGACTGGAGC GCTTGGAACA CCTGGCTGAG AAGTTCAGGC AGAAGGCCTC 
     4561 AACGCACGAG ACTTGGGCTT ATGGCAAAGA GCAGATCTTG CTGCAGAAGG ATTACGAGTC 
     4621 GGCGTCGCTG ACAGAGGTGC GGGCTCTGCT GCGGAAGCAC GAGGCGTTCG AGAGCGACCT 
     4681 GGCAGCGCAC CAGGACCGCG TGGAGCAGAT CGCAGCCATC GCGCAGGAGC TCAATGAACT 
     4741 GGACTATCAC GACGCTGTGA ATGTCAATGA TCGGTGCCAG AAAATTTGTG ACCAGTGGGA 
     4801 CCGACTGGGA ACGCTTACTC AGAAGAGGAG AGAAGCCCTA GAGAGAATGG AGAAATTGCT 
     4861 AGAAACCATT GATCAGCTTC ACCTGGAGTT TGCCAAGAGG GCTGCTCCTT TCAACAATTG 
     4921 GATGGAGGGC GCTATGGAGG ATCTGCAAGA TATGTTCATT GTCCACAGCA TTGAGGAGAT 
     4981 CCAGAGTCTG ATCACTGCGC ATGAGCAGTT CAAGGCCACG CTGCCCGAGG CGGACGGAGA 
     5041 GCGGCAGTCC ATCATGGCCA TCCAGAACGA GGTGGAGAAG GTGATTCAGA GCTACAACAT 
     5101 CAGAATCAGC TCAAGCAACC CGTACAGCAC TGTCACCATG GATGAGCTCC GGACCAAGTG 
     5161 GGACAAGGTG AAGCAACTCG TGCCCATCCG CGATCAATCC CTGCAGGAGG AGCTGGCTCG 
     5221 CCAGCATGCT AACGAGCGTC TGAGGCGCCA GTTTGCTGCC CAAGCCAATG CCATTGGGCC 
     5281 CTGGATCCAG AACAAGATGG AGGAGATTGC CCGGAGCTCC ATCCAGATCA CAGGAGCCCT 
     5341 GGAAGACCAG ATGAACCAGC TGAAGCAGTA TGAGCACAAC ATCATCAACT ATAAGAACAA 
     5401 CATCGACAAG CTGGAGGGAG ACCATCAGCT CATCCAGGAG GCCCTTGTCT TTGACAACAA 
     5461 GCACACGAAC TACACGATGG AGCACATTCG TGTTGGATGG GAGCTGCTGC TGACAACCAT 
     5521 CGCCAGAACC ATCAATGAGG TGGAGACTCA GATCCTGACG AGAGATGCGA AGGGCATCAC 
     5581 CCAGGAGCAG ATGAATGAGT TCAGAGCCTC CTTCAACCAC TTTGACAGGA GGAAGAATGG 
     5641 CCTGATGGAT CATGAGGATT TCAGAGCCTG CCTGATTTCC ATGGGTTATG ACCTGGGTGA 
     5701 AGCCGAATTT GCCCGCATTA TGACCCTGGT AGATCCCAAC GGGCAAGGCA CCGTCACCTT 
     5761 CCAATCCTTC ATCGACTTCA TGACTAGAGA GACGGCTGAC ACCGACACTG CCGAGCAGGT 
     5821 CATCGCCTCC TTCCGGATCC TGGCTTCTGA TAAGCCATAC ATCCTGGCGG AGGAGCTGCG 
     5881 TCGGGAGCTG CCCCCGGATC AGGCCCAGTA CTGCATCAAG AGGATGCCCG CCTACTCGGG 
     5941 CCCAGGCAGT GTGCCTGGTG CACTGGATTA CGCTGCGTTC TCTTCCGCAC TCTACGGGGA 
     6001 GAGCGATCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC 
     6061 AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG 
     6121 TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA 
     6181 GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT TCGATATCAA GCTTATCGAT 
     6241 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 
     6301 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 
     6361 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 
     6421 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 
     6481 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 
     6541 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 
     6601 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 
     6661 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 
     6721 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 
     6781 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 
     6841 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC 
     6901 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC 
     6961 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 
     7021 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA 
     7081 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC 
     7141 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA 
     7201 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA 
     7261 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT 
     7321 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT 
     7381 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 
     7441 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 
     7501 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA 
     7561 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     7621 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     7681 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     7741 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     7801 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     7861 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     7921 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     7981 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     8041 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     8101 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     8161 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     8221 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGATTACGC CCCGCCCTGC 
     8281 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 
     8341 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 
     8401 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA 
     8461 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA 
     8521 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC 
     8581 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG 
     8641 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC 
     8701 ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA 
     8761 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC 
     8821 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT 
     8881 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATACTCTT CCTTTTTCAA 
     8941 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     9001 TAGAAAAATA AACAAATAGG GGTCCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAC
//