LOCUS       RC208502                6972 bp    DNA     circular  SYN 
DEFINITION  PRKCG (Myc-DDK-tagged)-Human protein kinase C, gamma (PRKCG)
ACCESSION   NM_002739
VERSION     NM_002739.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3129..3220 
                     /label="Myc-DDK"  
     misc_feature    3250..3835 
                     /label="PolyA signal"  
     misc_feature    3307..3326 
                     /label="Restriction Enzyme"  
     misc_feature    4111..4783 
                     /label="ColE1"  
     misc_feature    5213..6007 
                     /label="Kan/Neo"  
     misc_feature    6042..6344 
                     /label="SV40 ori"  
     misc_feature    6430..6907 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3111..3117 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3111 
                     /label="PRKCG"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGTCTG GGCCCCGGCG TAGGCGATTC AGAGGGGGGA CCCCGGCCCC 
     1081 TGTTTTGCAG AAAGGGGGCC CTGAGGCAGA AGGTGGTCCA CGAAGTCAAG AGCCACAAGT 
     1141 TCACCGCTCG CTTCTTCAAG CAGCCCACCT TCTGCAGCCA CTGCACCGAC TTCATCTGGG 
     1201 GTATCGGAAA GCAGGGCCTG CAATGTCAAG TCTGCAGCTT TGTGGTTCAT CGACGATGCC 
     1261 ACGAATTTGT GACCTTCGAG TGTCCAGGCG CTGGGAAGGG CCCCCAGACG GATGACCCCC 
     1321 GGAACAAACA CAAGTTCCGC CTGCATAGCT ACAGCAGCCC CACCTTCTGC GACCACTGTG 
     1381 GCTCCCTCCT CTACGGGCTT GTGCACCAGG GCATGAAATG CTCCTGCTGC GAGATGAACG 
     1441 TGCACCGGCG CTGTGTGCGT AGCGTGCCCT CCCTGTGCGG TGTGGACCAC ACCGAGCGCC 
     1501 GCGGGCGCCT GCAGCTGGAG ATCCGGGCTC CCACAGCAGA TGAGATCCAC GTAACTGTTG 
     1561 GCGAGGCCCG TAACCTAATT CCTATGGACC CCAATGGTCT CTCTGATCCC TATGTGAAAC 
     1621 TGAAGCTCAT CCCAGACCCT CGGAACCTGA CGAAACAGAA GACCCGAACG GTGAAAGCCA 
     1681 CGCTAAACCC TGTGTGGAAT GAGACCTTTG TGTTCAACCT GAAGCCAGGG GATGTGGAGC 
     1741 GCCGGCTCAG CGTGGAGGTG TGGGACTGGG ACCGGACCTC CCGCAACGAC TTCATGGGGG 
     1801 CCATGTCCTT TGGCGTCTCG GAGCTGCTCA AGGCGCCCGT GGATGGCTGG TACAAGTTAC 
     1861 TGAACCAGGA GGAGGGCGAG TATTACAATG TGCCGGTGGC CGATGCTGAC AACTGCAGCC 
     1921 TCCTCCAGAA GTTTGAGGCT TGTAACTACC CCCTGGAATT GTATGAGCGG GTGCGGATGG 
     1981 GCCCCTCTTC CTCTCCCATC CCCTCCCCTT CCCCTAGTCC CACCGACCCC AAGCGCTGCT 
     2041 TCTTCGGGGC GAGTCCAGGA CGCCTGCACA TCTCCGACTT CAGCTTCCTC ATGGTTCTAG 
     2101 GAAAAGGCAG TTTTGGGAAG GTGATGCTGG CCGAGCGCAG GGGCTCTGAT GAGCTCTACG 
     2161 CCATCAAGAT CTTGAAAAAG GACGTGATCG TCCAGGACGA CGATGTGGAC TGCACGCTGG 
     2221 TGGAGAAACG TGTGCTGGCG CTGGGGGGCC GGGGTCCTGG CGGCCGGCCC CACTTCCTCA 
     2281 CCCAGCTCCA CTCCACCTTC CAGACCCCGG ACCGCCTGTA TTTCGTGATG GAGTACGTCA 
     2341 CCGGGGGAGA CTTGATGTAC CACATTCAAC AGCTGGGCAA GTTTAAGGAG CCCCATGCAG 
     2401 CGTTCTACGC GGCAGAAATC GCTATCGGCC TCTTCTTCCT TCACAATCAG GGCATCATCT 
     2461 ACAGGGACCT GAAGCTGGAC AATGTGATGC TGGATGCTGA GGGACACATC AAGATCACTG 
     2521 ACTTTGGCAT GTGTAAGGAG AACGTCTTCC CCGGGACGAC AACCCGCACC TTCTGCGGGA 
     2581 CCCCGGACTA CATAGCCCCG GAGATCATTG CCTACCAGCC CTATGGGAAG TCTGTCGATT 
     2641 GGTGGTCCTT TGGAGTTCTG CTGTATGAGA TGTTGGCAGG ACAGCCTCCC TTCGATGGGG 
     2701 AGGACGAGGA GGAGCTGTTT CAGGCCATCA TGGAACAAAC TGTCACCTAC CCCAAGTCGC 
     2761 TTTCCCGGGA AGCCGTGGCC ATCTGCAAGG GGTTCCTGAC CAAGCACCCA GGGAAGCGCC 
     2821 TGGGCTCAGG GCCTGATGGG GAACCTACCA TCCGTGCACA TGGCTTTTTC CGCTGGATTG 
     2881 ACTGGGAGCG GCTGGAACGA TTGGAGATCC CGCCTCCTTT CAGACCCCGC CCGTGTGGCC 
     2941 GCAGCGGCGA GAACTTTGAC AAGTTCTTCA CGCGGGCGGC GCCAGCGCTG ACCCCTCCAG 
     3001 ACCGCCTAGT CCTGGCCAGC ATCGACCAGG CCGATTTCCA GGGCTTCACC TACGTGAACC 
     3061 CCGACTTCGT GCACCCGGAT GCCCGCAGCC CCACCAGCCC AGTGCCTGTG CCCGTCATGA 
     3121 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3181 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3241 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3301 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3361 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3421 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3481 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3541 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3601 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3661 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3721 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     3781 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     3841 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     3901 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     3961 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4021 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4081 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4141 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4201 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4261 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4321 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4381 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4441 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4501 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4561 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4621 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4681 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4741 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     4801 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     4861 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     4921 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     4981 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5041 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5101 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5161 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5221 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5281 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5341 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5401 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5461 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5521 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5581 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5641 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5701 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     5761 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     5821 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     5881 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     5941 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6001 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6061 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6121 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6181 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6241 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6301 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6361 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6421 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6481 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6541 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6601 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6661 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6721 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     6781 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     6841 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     6901 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     6961 AACGCGAATT TT
//