LOCUS       RC208450                8253 bp    DNA     circular  SYN 
DEFINITION  MICAL2 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 2 (MICAL2)
ACCESSION   NM_014632
VERSION     NM_014632.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4410..4501 
                     /label="Myc-DDK"  
     misc_feature    4531..5116 
                     /label="PolyA signal"  
     misc_feature    4588..4607 
                     /label="Restriction Enzyme"  
     misc_feature    5392..6064 
                     /label="ColE1"  
     misc_feature    6494..7288 
                     /label="Kan/Neo"  
     misc_feature    7323..7625 
                     /label="SV40 ori"  
     misc_feature    7711..8188 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4392..4398 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4392 
                     /label="MICAL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGAAAAC GAGGATGAGA AGCAGGCCCA GGCGGGGCAG GTTTTTGAGA 
     1081 ACTTTGTCCA GGCATCCACG TGCAAAGGTA CCCTCCAGGC CTTCAACATT CTCACACGAC 
     1141 ACCTGGACCT AGACCCTCTG GACCACAGAA ACTTTTATTC CAAGCTCAAG TCCAAGGTGA 
     1201 CCACCTGGAA AGCCAAAGCC CTGTGGTACA AATTGGATAA GCGTGGTTCC CACAAAGAGT 
     1261 ATAAGCGAGG GAAGTCGTGC ACGAACACCA AGTGTCTCAT AGTTGGGGGA GGACCCTGTG 
     1321 GCTTGCGCAC TGCCATTGAA CTTGCCTACC TGGGAGCCAA AGTGGTCGTG GTGGAGAAGA 
     1381 GGGACTCCTT CTCCCGGAAC AACGTGCTAC ACCTCTGGCC TTTCACCATC CATGACCTTC 
     1441 GGGGCCTGGG AGCCAAGAAG TTCTATGGGA AGTTCTGTGC TGGCTCCATC GACCATATCA 
     1501 GTATTCGCCA ACTACAGCTC ATCCTATTCA AGGTGGCCCT GATGCTGGGA GTTGAAATCC 
     1561 ATGTGAATGT GGAGTTCGTG AAGGTTCTAG AGCCTCCTGA AGATCAAGAA AATCAAAAAA 
     1621 TTGGCTGGCG GGCAGAATTT CTCCCTACAG ACCATTCTCT GTCGGAGTTT GAGTTTGACG 
     1681 TCATCATTGG TGCCGATGGC CGCAGGAACA CCCTGGAAGG GTTCAGAAGA AAAGAATTCC 
     1741 GTGGGAAGCT GGCGATTGCC ATCACCGCCA ACTTCATAAA CAGAAACAGC ACAGCGGAAG 
     1801 CCAAGGTGGA AGAGATTAGT GGTGTGGCTT TCATCTTCAA TCAGAAATTT TTTCAGGACC 
     1861 TTAAAGAAGA AACAGGCATA GATCTTGAGA ACATTGTTTA CTACAAGGAC TGCACCCACT 
     1921 ATTTTGTAAT GACAGCCAAG AAGCAGAGCC TGCTCGACAA AGGTGTCATC ATTAACGACT 
     1981 ACATCGACAC AGAGATGCTG CTGTGTGCGG AGAACGTGAA CCAAGACAAC CTGCTATCCT 
     2041 ATGCCCGGGA AGCTGCAGAC TTTGCCACCA ACTACCAGCT GCCATCCTTA GACTTTGCCA 
     2101 TGAACCACTA TGGGCAGCCT GATGTGGCCA TGTTTGACTT TACCTGCATG TATGCCTCAG 
     2161 AGAACGCGGC CCTGGTGCGG GAGCGGCAGG CGCACCAGCT GCTCGTGGCC CTTGTGGGTG 
     2221 ACAGCTTGCT TGAGCCATTT TGGCCCATGG GTACAGGCTG TGCCCGTGGC TTCCTGGCAG 
     2281 CCTTTGACAC GGCATGGATG GTGAAGAGCT GGAACCAGGG CACCCCTCCC CTGGAGCTGC 
     2341 TGGCTGAAAG GGAAAGTCTC TACCGGCTGT TACCTCAGAC AACCCCGGAG AACATCAACA 
     2401 AGAACTTTGA GCAGTACACG TTGGACCCAG GGACACGGTA CCCAAACCTC AACTCACACT 
     2461 GTGTCAGGCC CCATCAGGTG AAGCATTTGT ATATCACTAA GGAGCTGGAG CACTACCCTC 
     2521 TCGAGAGACT GGGCTCGGTG AGGAGATCTG TCAACCTCTC CAGGAAGGAG TCAGATATCC 
     2581 GGCCCAGCAA GCTCCTGACC TGGTGCCAGC AGCAGACAGA GGGCTACCAG CATGTCAACG 
     2641 TCACCGACCT GACCACATCC TGGCGCAGTG GGTTGGCCCT GTGTGCCATC ATCCACCGCT 
     2701 TCCGGCCTGA GCTCATCAAC TTTGACTCTT TGAATGAAGA TGATGCTGTG GAGAACAACC 
     2761 AGCTCGCATT TGATGTGGCC GAGCGAGAGT TTGGGATCCC TCCAGTGACC ACGGGCAAAG 
     2821 AGATGGCATC TGCCCAGGAG CCTGACAAGC TCAGCATGGT CATGTACCTC TCCAAGTTCT 
     2881 ACGAGCTCTT CCGGGGCACC CCACTGAGGC CCGTGGATTC TTGGCGCAAA AACTATGGAG 
     2941 AAAATGCTGA CCTCAGCTTG GCCAAATCAT CCATTTCTAA TAACTATCTC AACCTCACAT 
     3001 TTCCAAGGAA GAGGACTCCA CGGGTGGATG GTCAAACCGG AGAGAATGAC ATGAACAAAC 
     3061 GGAGACGGAA GGGCTTCACC AACCTGGACG AGCCTTCAAA CTTTTCCAGC CGTAGCTTGG 
     3121 GCTCCAATCA AGAGTGTGGG AGCAGTAAGG AAGGTGGAAA TCAGAACAAA GTCAAGTCCA 
     3181 TGGCGAATCA GCTGCTGGCC AAGTTTGAGG AGAGCACTCG GAACCCCTCA CTCATGAAGC 
     3241 AGGAACGCCG TGTCTCAGGG ATAGGTAAGC CGGTCCTGTG CTCTTCCTCC GGCCCTCCTG 
     3301 TTCACTCTTG CTGCCCCAAG CCGGAGGAGG CCACACCCAG CCCATCACCT CCTCTGAAAA 
     3361 GGCAGTTCCC CTCTGTGGTC GTGACGGGGC ACGTGCTCAG AGAGCTCAAG CAAGTGTCTG 
     3421 CTGGCAGTGA GTGCCTGAGC AGACCTTGGA GAGCCAGAGC CAAGTCTGAC CTACAGCTGG 
     3481 GTGGGACAGA AAATTTCGCT ACCCTGCCTT CTACCCGCCC GAGGGCGCAG GCTCTTTCCG 
     3541 GGGTGCTGTG GCGGCTGCAG CAAGTGGAGG AAAAGATTCT CCAGAAGAGG GCTCAGAACT 
     3601 TGGCCAACAG GGAATTTCAC ACAAAGAACA TTAAGGAGAA GGCGGCTCAC CTTGCCTCCA 
     3661 TGTTTGGACA CGGGGATTTC CCGCAGAATA AACTACTCTC TAAAGGCCTG TCTCATACTC 
     3721 ATCCTCCATC TCCTCCCTCT CGCCTTCCGT CTCCTGATCC AGCTGCTTCT TCCTCTCCAT 
     3781 CAACTGTTGA CTCTGCTTCT CCTGCCAGAA AGGAAAAGAA GTCACCTTCA GGGTTCCATT 
     3841 TTCATCCCAG CCATTTGAGA ACAGTGCATC CTCAGCTGAC GGTAGGGAAA GTGTCCAGCG 
     3901 GAATAGGGGC TGCAGCTGAA GTCCTGGTCA ATCTGTACAT GAATGATCAC AGACCTAAGG 
     3961 CCCAGGCCAC CTCTCCAGAC CTGGAATCTA TGCGAAAGTC ATTTCCCCTT AACCTGGGAG 
     4021 GCAGCGACAC GTGTTACTTC TGTAAGAAAC GTGTGTACGT GATGGAACGG CTGAGCGCCG 
     4081 AGGGCCACTT CTTCCACCGG GAGTGTTTCC GCTGCAGCAT CTGTGCCACC ACCTTGCGCC 
     4141 TGGCCGCCTA CACCTTTGAC TGCGATGAAG GCAAATTTTA CTGCAAGCCT CACTTCATTC 
     4201 ACTGTAAAAC CAATAGCAAA CAACGGAAGA GACGGGCAGA GTTGAAGCAA CAAAGAGAGG 
     4261 AGGAGGCAAC ATGGCAAGAG CAGGAAGCCC CTCGGAGAGA CACTCCCACC GAAAGTTCTT 
     4321 GCGCAGTGGC CGCCATTGGC ACCCTGGAAG GCAGCCCCCC AGTTCATTTC AGCCTTCCAG 
     4381 TGCTACACCC ACTTCTTGGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4441 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4501 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4561 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4621 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4681 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4741 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4801 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4861 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4921 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4981 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5041 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5101 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5161 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5221 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5281 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5341 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5401 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5461 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5521 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5581 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5641 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5701 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5761 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5821 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5881 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5941 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6001 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6061 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6121 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6181 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6241 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6301 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6361 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6421 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6481 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6541 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6601 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6661 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6721 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6781 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6841 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6901 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6961 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7021 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7081 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7141 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7201 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7261 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7321 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7381 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7441 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7501 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7681 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7741 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7801 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7861 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7921 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7981 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8041 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8101 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8161 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8221 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//