LOCUS       RC208408                7764 bp    DNA     circular  SYN 
DEFINITION  THBS4 (Myc-DDK-tagged)-Human thrombospondin 4 (THBS4)
ACCESSION   NM_003248
VERSION     NM_003248.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3921..4012 
                     /label="Myc-DDK"  
     misc_feature    4042..4627 
                     /label="PolyA signal"  
     misc_feature    4099..4118 
                     /label="Restriction Enzyme"  
     misc_feature    4903..5575 
                     /label="ColE1"  
     misc_feature    6005..6799 
                     /label="Kan/Neo"  
     misc_feature    6834..7136 
                     /label="SV40 ori"  
     misc_feature    7222..7699 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3903..3909 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3903 
                     /label="THBS4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGCCCCG CGCGGAGCCG CCGTCCTCCT GCTGCACCTG GTCCTGCAGC 
     1081 GGTGGCTAGC GGCAGGCGCC CAGGCCACCC CCCAGGTCTT TGACCTTCTC CCATCTTCCA 
     1141 GTCAGAGGCT AAACCCAGGC GCTCTGCTGC CAGTCCTGAC AGACCCCGCC CTGAATGATC 
     1201 TCTATGTGAT TTCCACCTTC AAGCTGCAGA CTAAAAGTTC AGCCACCATC TTCGGTCTTT 
     1261 ACTCTTCAAC TGACAACAGT AAATATTTTG AATTTACTGT GATGGGACGC TTAAACAAAG 
     1321 CCATCCTCCG TTACCTGAAG AACGATGGGA AGGTGCATTT GGTGGTTTTC AACAACCTGC 
     1381 AGCTGGCAGA CGGAAGGCGG CACAGGATCC TCCTGAGGCT GAGCAATTTG CAGCGAGGGG 
     1441 CCGGCTCCCT AGAGCTCTAC CTGGACTGCA TCCAGGTGGA TTCCGTTCAC AATCTCCCCA 
     1501 GGGCCTTTGC TGGCCCCTCC CAGAAACCTG AGACCATTGA ATTGAGGACT TTCCAGAGGA 
     1561 AGCCACAGGA CTTCTTGGAA GAGCTGAAGC TGGTGGTGAG AGGCTCACTG TTCCAGGTGG 
     1621 CCAGCCTGCA AGACTGCTTC CTGCAGCAGA GTGAGCCACT GGCTGCCACA GGCACAGGGG 
     1681 ACTTTAACCG GCAGTTCTTG GGTCAAATGA CACAATTAAA CCAACTCCTG GGAGAGGTGA 
     1741 AGGACCTTCT GAGACAGCAG GTTAAGGAAA CATCATTTTT GCGAAACACC ATAGCTGAAT 
     1801 GCCAGGCTTG CGGTCCTCTC AAGTTTCAGT CTCCGACCCC AAGCACGGTG GTGCCCCCGG 
     1861 CTCCCCCTGC ACCGCCAACA CGCCCACCTC GTCGGTGTGA CTCCAACCCA TGTTTCCGAG 
     1921 GTGTCCAATG TACCGACAGT AGAGATGGCT TCCAGTGTGG GCCCTGCCCC GAGGGCTACA 
     1981 CAGGAAACGG GATCACCTGT ATTGATGTTG ATGAGTGCAA ATACCATCCC TGCTACCCGG 
     2041 GCGTGCACTG CATAAATTTG TCTCCTGGCT TCAGATGTGA CGCCTGCCCA GTGGGCTTCA 
     2101 CAGGGCCCAT GGTGCAGGGT GTTGGGATCA GTTTTGCCAA GTCAAACAAG CAGGTCTGCA 
     2161 CTGACATTGA TGAGTGTCGA AATGGAGCGT GCGTTCCCAA CTCGATCTGC GTTAATACTT 
     2221 TGGGATCTTA CCGCTGTGGG CCTTGTAAGC CGGGGTATAC TGGTGATCAG ATAAGGGGAT 
     2281 GCAAAGCGGA AAGAAACTGC AGAAACCCAG AGCTGAACCC TTGCAGTGTG AATGCCCAGT 
     2341 GCATTGAAGA GAGGCAGGGG GATGTGACAT GTGTGTGTGG AGTCGGTTGG GCTGGAGATG 
     2401 GCTATATCTG TGGAAAGGAT GTGGACATCG ACAGTTACCC CGACGAAGAA CTGCCATGCT 
     2461 CTGCCAGGAA CTGTAAAAAG GACAACTGCA AATATGTGCC AAATTCTGGC CAAGAAGATG 
     2521 CAGACAGAGA TGGCATTGGC GACGCTTGTG ACGAGGATGC TGACGGAGAT GGGATCCTGA 
     2581 ATGAGCAGGA TAACTGTGTC CTGATTCATA ATGTGGACCA AAGGAACAGC GATAAAGATA 
     2641 TCTTTGGGGA TGCCTGTGAT AACTGCCTGA GTGTCTTAAA TAACGACCAG AAAGACACCG 
     2701 ATGGGGATGG AAGAGGAGAT GCCTGTGATG ATGACATGGA TGGAGATGGA ATAAAAAACA 
     2761 TTCTGGACAA CTGCCCAAAA TTTCCCAATC GTGACCAACG GGACAAGGAT GGTGATGGTG 
     2821 TGGGGGATGC CTGTGACAGT TGTCCTGATG TCAGCAACCC TAACCAGTCT GATGTGGATA 
     2881 ATGATCTGGT TGGGGACTCC TGTGACACCA ATCAGGACAG TGATGGAGAT GGGCACCAGG 
     2941 ACAGCACAGA CAACTGCCCC ACCGTCATTA ACAGTGCCCA GCTGGACACC GATAAGGATG 
     3001 GAATTGGTGA CGAGTGTGAT GATGATGATG ACAATGATGG TATCCCAGAC CTGGTGCCCC 
     3061 CTGGACCAGA CAACTGCCGG CTGGTCCCCA ACCCAGCCCA GGAGGATAGC AACAGCGACG 
     3121 GAGTGGGAGA CATCTGTGAG TCTGACTTTG ACCAGGACCA GGTCATCGAT CGGATCGACG 
     3181 TCTGCCCAGA GAACGCAGAG GTCACCCTGA CCGACTTCAG GGCTTACCAG ACCGTGGTCC 
     3241 TGGATCCTGA AGGGGATGCC CAGATCGATC CCAACTGGGT GGTCCTGAAC CAGGGCATGG 
     3301 AGATTGTACA GACCATGAAC AGTGATCCTG GCCTGGCAGT GGGGTACACA GCTTTTAATG 
     3361 GAGTTGACTT CGAAGGGACC TTCCATGTGA ATACCCAGAC AGATGATGAC TATGCAGGCT 
     3421 TTATCTTTGG CTACCAAGAT AGCTCCAGCT TCTACGTGGT CATGTGGAAG CAGACGGAGC 
     3481 AGACATATTG GCAAGCCACC CCATTCCGAG CAGTTGCAGA ACCTGGCATT CAGCTCAAGG 
     3541 CTGTGAAGTC TAAGACAGGT CCAGGGGAGC ATCTCCGGAA CTCCCTGTGG CACACGGGGG 
     3601 ACACCAGTGA CCAGGTCAGG CTGCTGTGGA AGGACTCCAG GAATGTGGGC TGGAAGGACA 
     3661 AGGTGTCCTA CCGCTGGTTC CTACAGCACA GGCCCCAGGT GGGCTACATC AGGGTACGAT 
     3721 TTTATGAAGG CTCTGAGTTG GTGGCTGACT CTGGCGTCAC CATAGACACC ACAATGCGTG 
     3781 GAGGCCGACT TGGCGTTTTC TGCTTCTCTC AAGAAAACAT CATCTGGTCC AACCTCAAGT 
     3841 ATCGCTGCAA TGACACCATC CCTGAGGACT TCCAAGAGTT TCAAACCCAG AATTTCGACC 
     3901 GCTTCGATAA TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3961 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4021 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4081 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4141 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4201 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4261 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4321 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4381 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4441 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4501 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4561 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4621 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4681 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4741 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4801 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4861 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4921 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4981 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5041 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5101 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5161 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5221 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5281 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5341 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5401 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5461 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5521 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5581 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5641 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5701 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5761 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5821 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5881 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5941 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6001 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6061 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6121 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6181 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6241 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6301 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6361 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6421 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6481 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6541 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6601 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6661 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6721 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6781 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6841 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6901 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6961 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7021 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7081 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7201 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7261 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7321 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7381 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7441 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7501 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7561 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7621 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7681 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7741 TAACAAAAAT TTAACGCGAA TTTT
//