LOCUS       RC208404                8331 bp    DNA     circular  SYN 
DEFINITION  SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2
ACCESSION   NM_004176
VERSION     NM_004176.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4470 
                     /label="SREBF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GACGAGCCAC CCTTCAGCGA GGCGGCTTTG GAGCAGGCGC 
     1081 TGGGCGAGCC GTGCGATCTG GACGCGGCGC TGCTGACCGA CATCGAAGAC ATGCTTCAGC 
     1141 TTATCAACAA CCAAGACAGT GACTTCCCTG GCCTATTTGA CCCACCCTAT GCTGGGAGTG 
     1201 GGGCAGGGGG CACAGACCCT GCCAGCCCCG ATACCAGCTC CCCAGGCAGC TTGTCTCCAC 
     1261 CTCCTGCCAC ATTGAGCTCC TCTCTTGAAG CCTTCCTGAG CGGGCCGCAG GCAGCGCCCT 
     1321 CACCCCTGTC CCCTCCCCAG CCTGCACCCA CTCCATTGAA GATGTACCCG TCCATGCCCG 
     1381 CTTTCTCCCC TGGGCCTGGT ATCAAGGAAG AGTCAGTGCC ACTGAGCATC CTGCAGACCC 
     1441 CCACCCCACA GCCCCTGCCA GGGGCCCTCC TGCCACAGAG CTTCCCAGCC CCAGCCCCAC 
     1501 CGCAGTTCAG CTCCACCCCT GTGTTAGGCT ACCCCAGCCC TCCGGGAGGC TTCTCTACAG 
     1561 GAAGCCCTCC CGGGAACACC CAGCAGCCGC TGCCTGGCCT GCCACTGGCT TCCCCGCCAG 
     1621 GGGTCCCGCC CGTCTCCTTG CACACCCAGG TCCAGAGTGT GGTCCCCCAG CAGCTACTGA 
     1681 CAGTCACAGC TGCCCCCACG GCAGCCCCTG TAACGACCAC TGTGACCTCG CAGATCCAGC 
     1741 AGGTCCCGGT CCTGCTGCAG CCCCACTTCA TCAAGGCAGA CTCGCTGCTT CTGACAGCCA 
     1801 TGAAGACAGA CGGAGCCACT GTGAAGGCGG CAGGTCTCAG TCCCCTGGTC TCTGGCACCA 
     1861 CTGTGCAGAC AGGGCCTTTG CCGACCCTGG TGAGTGGCGG AACCATCTTG GCAACAGTCC 
     1921 CACTGGTCGT AGATGCGGAG AAGCTGCCTA TCAACCGGCT CGCAGCTGGC AGCAAGGCCC 
     1981 CGGCCTCTGC CCAGAGCCGT GGAGAGAAGC GCACAGCCCA CAACGCCATT GAGAAGCGCT 
     2041 ACCGCTCCTC CATCAATGAC AAAATCATTG AGCTCAAGGA TCTGGTGGTG GGCACTGAGG 
     2101 CAAAGCTGAA TAAATCTGCT GTCTTGCGCA AGGCCATCGA CTACATTCGC TTTCTGCAAC 
     2161 ACAGCAACCA GAAACTCAAG CAGGAGAACC TAAGTCTGCG CACTGCTGTC CACAAAAGCA 
     2221 AATCTCTGAA GGATCTGGTG TCGGCCTGTG GCAGTGGAGG GAACACAGAC GTGCTCATGG 
     2281 AGGGCGTGAA GACTGAGGTG GAGGACACAC TGACCCCACC CCCCTCGGAT GCTGGCTCAC 
     2341 CTTTCCAGAG CAGCCCCTTG TCCCTTGGCA GAAGGGGCAG TGGCAGCGGT GGCAGTGGCA 
     2401 GTGACTCGGA GCCTGACAGC CCAGTCTTTG AGGACAGCAA GGCAAAGCCA GAGCAGCGGC 
     2461 CGTCTCTGCA CAGCCGGGGC ATGCTGGACC GCTCCCGCCT GGCCCTGTGC ACGCTCGTCT 
     2521 TCCTCTGCCT GTCCTGCAAC CCCTTGGCCT CCTTGCTGGG GGCCCGGGGG CTTCCCAGCC 
     2581 CCTCAGATAC CACCAGCGTC TACCATAGCC CTGGGCGCAA CGTGCTGGGC ACCGAGAGCA 
     2641 GAGATGGCCC TGGCTGGGCC CAGTGGCTGC TGCCCCCAGT GGTCTGGCTG CTCAATGGGC 
     2701 TGTTGGTGCT CGTCTCCTTG GTGCTTCTCT TTGTCTACGG TGAGCCAGTC ACACGGCCCC 
     2761 ACTCAGGCCC CGCCGTGTAC TTCTGGAGGC ATCGCAAGCA GGCTGACCTG GACCTGGCCC 
     2821 GGGGAGACTT TGCCCAGGCT GCCCAGCAGC TGTGGCTGGC CCTGCGGGCA CTGGGCCGGC 
     2881 CCCTGCCCAC CTCCCACCTG GACCTGGCTT GTAGCCTCCT CTGGAACCTC ATTCGTCACC 
     2941 TGCTGCAGCG TCTCTGGGTG GGCCGCTGGC TGGCAGGCCG GGCAGGGGGC CTGCAGCAGG 
     3001 ACTGTGCTCT GCGAGTGGAT GCTAGCGCCA GCGCCCGAGA CGCAGCCCTG GTCTACCATA 
     3061 AGCTGCACCA GCTGCACACC ATGGGGAAGC ACACAGGCGG GCACCTCACT GCCACCAACC 
     3121 TGGCGCTGAG TGCCCTGAAC CTGGCAGAGT GTGCAGGGGA TGCCGTGTCT GTGGCGACGC 
     3181 TGGCCGAGAT CTATGTGGCG GCTGCATTGA GAGTGAAGAC CAGTCTCCCA CGGGCCTTGC 
     3241 ATTTTCTGAC ACGCTTCTTC CTGAGCAGTG CCCGCCAGGC CTGCCTGGCA CAGAGTGGCT 
     3301 CAGTGCCTCC TGCCATGCAG TGGCTCTGCC ACCCCGTGGG CCACCGTTTC TTCGTGGATG 
     3361 GGGACTGGTC CGTGCTCAGT ACCCCATGGG AGAGCCTGTA CAGCTTGGCC GGGAACCCAG 
     3421 TGGACCCCCT GGCCCAGGTG ACTCAGCTAT TCCGGGAACA TCTCTTAGAG CGAGCACTGA 
     3481 ACTGTGTGAC CCAGCCCAAC CCCAGCCCTG GGTCAGCTGA TGGGGACAAG GAATTCTCGG 
     3541 ATGCCCTCGG GTACCTGCAG CTGCTGAACA GCTGTTCTGA TGCTGCGGGG GCTCCTGCCT 
     3601 ACAGCTTCTC CATCAGTTCC AGCATGGCCA CCACCACCGG CGTAGACCCG GTGGCCAAGT 
     3661 GGTGGGCCTC TCTGACAGCT GTGGTGATCC ACTGGCTGCG GCGGGATGAG GAGGCGGCTG 
     3721 AGCGGCTGTG CCCGCTGGTG GAGCACCTGC CCCGGGTGCT GCAGGAGTCT GAGAGACCCC 
     3781 TGCCCAGGGC AGCTCTGCAC TCCTTCAAGG CTGCCCGGGC CCTGCTGGGC TGTGCCAAGG 
     3841 CAGAGTCTGG TCCAGCCAGC CTGACCATCT GTGAGAAGGC CAGTGGGTAC CTGCAGGACA 
     3901 GCCTGGCTAC CACACCAGCC AGCAGCTCCA TTGACAAGGC CGTGCAGCTG TTCCTGTGTG 
     3961 ACCTGCTTCT TGTGGTGCGC ACCAGCCTGT GGCGGCAGCA GCAGCCCCCG GCCCCGGCCC 
     4021 CAGCAGCCCA GGGCACCAGC AGCAGGCCCC AGGCTTCCGC CCTTGAGCTG CGTGGCTTCC 
     4081 AACGGGACCT GAGCAGCCTG AGGCGGCTGG CACAGAGCTT CCGGCCCGCC ATGCGGAGGG 
     4141 TGTTCCTACA TGAGGCCACG GCCCGGCTGA TGGCGGGGGC CAGCCCCACA CGGACACACC 
     4201 AGCTCCTCGA CCGCAGTCTG AGGCGGCGGG CAGGCCCCGG TGGCAAAGGA GGCGCGGTGG 
     4261 CGGAGCTGGA GCCGCGGCCC ACGCGGCGGG AGCACGCGGA GGCCTTGCTG CTGGCCTCCT 
     4321 GCTACCTGCC CCCCGGCTTC CTGTCGGCGC CCGGGCAGCG CGTGGGCATG CTGGCTGAGG 
     4381 CGGCGCGCAC ACTCGAGAAG CTTGGCGATC GCCGGCTGCT GCACGACTGT CAGCAGATGC 
     4441 TCATGCGCCT GGGCGGTGGG ACCACTGTCA CTTCCAGCAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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