LOCUS       RC208403                8541 bp    DNA     circular  SYN 
DEFINITION  DHX8 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8)
ACCESSION   NM_004941
VERSION     NM_004941.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4698..4789 
                     /label="Myc-DDK"  
     misc_feature    4819..5404 
                     /label="PolyA signal"  
     misc_feature    4876..4895 
                     /label="Restriction Enzyme"  
     misc_feature    5680..6352 
                     /label="ColE1"  
     misc_feature    6782..7576 
                     /label="Kan/Neo"  
     misc_feature    7611..7913 
                     /label="SV40 ori"  
     misc_feature    7999..8476 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4680..4686 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4680 
                     /label="DHX8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTGGCT GTAGCCATGG CGGGAGCCTT AATCGGGTCG GAGCCAGGCC 
     1081 CCGCGGAAGA ACTTGCCAAA CTCGAGTACC TGTCTTTGGT GTCAAAGGTT TGCACTGAGC 
     1141 TGGACAATCA CTTGGGGATC AACGACAAGG ACCTTGCTGA ATTTGTGATC AGTCTTGCTG 
     1201 AGAAAAATAC CACCTTTGAT ACTTTTAAGG CTTCTCTCGT CAAAAATGGT GCAGAATTTA 
     1261 CGGATTCTCT TATTAGTAAC TTGCTGCGTC TCATACAAAC CATGCGGCCT CCAGCGAAGC 
     1321 CTTCCACTAG CAAAGATCCA GTTGTTAAAC CTAAAACAGA AAAAGAAAAG CTGAAGGAAC 
     1381 TCTTCCCAGT CCTTTGCCAA CCGGACAACC CTTCTGTTCG GACCATGTTG GATGAAGATG 
     1441 ATGTGAAAGT TGCTGTGGAT GTCCTGAAAG AACTGGAAGC TTTAATGCCC AGCGCAGCAG 
     1501 GCCAGGAGAA GCAAAGAGAT GCTGAACACC GGGACAGGAC AAAGAAGAAG AAGCGGAGTC 
     1561 GAAGCCGAGA TCGAAACCGA GATCGAGACA GAGATAGGGA ACGAAACCGA GATAGAGACC 
     1621 ACAAGCGGAG ACACCGATCC CGCTCTCGAT CACGTTCCAG GACCCGGGAG AGGAATAAAG 
     1681 TGAAGTCTAG ATATCGGTCC AGGAGCAGGA GTCAGAGTCC CCCCAAAGAC CGGAAGGACC 
     1741 GGGACAAATA TGGAGAGCGG AATCTGGATA GATGGCGGGA TAAGCATGTG GACCGCCCTC 
     1801 CTCCAGAAGA GCCCACCATT GGTGACATTT ATAATGGCAA AGTTACCAGC ATCATGCAGT 
     1861 TTGGTTGCTT TGTGCAGCTG GAAGGACTAA GGAAGCGGTG GGAAGGCCTG GTGCACATCT 
     1921 CTGAGCTCCG GCGGGAGGGT CGTGTGGCCA ATGTAGCTGA TGTCGTGAGC AAAGGCCAGA 
     1981 GGGTCAAAGT CAAAGTGCTG TCCTTCACTG GGACCAAGAC CAGCCTGAGC ATGAAGGATG 
     2041 TGGATCAAGA GACTGGAGAA GATCTAAACC CAAATAGACG GCGAAATCTT GTCGGGGAGA 
     2101 CCAATGAGGA GACCTCAATG CGGAATCCTG ATAGACCCAC TCACTTGTCC CTTGTCAGTG 
     2161 CTCCTGAAGT AGAGGACGAC TCACTGGAAC GCAAGCGCCT CACCCAAATC TCTGACCCAG 
     2221 AGAAGTGGGA GATCAAACAG ATGATTGCTG CCAATGTCCT TTCCAAAGAA GAATTTCCAG 
     2281 ACTTTGATGA AGAGACTGGC ATTCTCCCTA AGGTGGATGA TGAAGAAGAT GAGGACCTTG 
     2341 AGATTGAATT GGTTGAGGAA GAGCCTCCAT TCCTGAGAGG GCACACTAAG CAAAGCATGG 
     2401 ACATGAGCCC CATTAAAATT GTCAAGAACC CAGACGGCTC CCTCTCCCAA GCAGCAATGA 
     2461 TGCAGAGTGC CTTGGCCAAA GAAAGGCGGG AACTCAAACA GGCCCAGCGG GAAGCTGAGA 
     2521 TGGATTCTAT TCCCATGGGA CTCAACAAAC ACTGGGTTGA CCCTCTGCCT GATGCGGAAG 
     2581 GCAGACAGAT TGCTGCCAAC ATGAGGGGTA TTGGGATGAT GCCCAATGAT ATTCCTGAGT 
     2641 GGAAGAAGCA TGCCTTTGGG GGCAACAAAG CCTCTTACGG AAAAAAGACC CAGATGTCAA 
     2701 TCCTTGAGCA GAGGGAGAGC CTGCCCATCT ACAAACTGAA GGAGCAATTG GTCCAGGCCG 
     2761 TCCATGACAA TCAGATCCTG ATTGTCATTG GTGAGACAGG ATCTGGAAAG ACAACACAGA 
     2821 TCACCCAGTA CCTGGCGGAG GCAGGCTACA CTTCCAGGGG CAAGATTGGG TGTACCCAGC 
     2881 CCAGAAGAGT GGCAGCTATG TCGGTGGCCA AAAGAGTGTC AGAGGAGTTT GGTTGTTGCT 
     2941 TAGGCCAAGA GGTGGGCTAC ACCATTCGAT TTGAGGACTG CACTAGCCCT GAAACAGTCA 
     3001 TCAAGTACAT GACAGATGGG ATGTTGCTTA GAGAGTGCTT GATTGACCCT GACCTCACTC 
     3061 AGTACGCGAT CATCATGTTG GACGAGGCAC ATGAGAGGAC AATTCACACT GATGTGCTCT 
     3121 TTGGATTGTT GAAAAAGACA GTTCAGAAAC GGCAGGACAT GAAGCTGATT GTCACCTCAG 
     3181 CCACCTTGGA TGCAGTGAAG TTTTCTCAAT ACTTCTATGA AGCTCCCATT TTCACCATCC 
     3241 CAGGTCGAAC ATATCCAGTG GAAATACTGT ACACAAAGGA ACCTGAGACA GATTATCTGG 
     3301 ATGCCAGCCT GATTACTGTT ATGCAGATTC ATTTAACAGA ACCACCAGGT GATATCCTGG 
     3361 TCTTCCTGAC TGGTCAGGAA GAAATTGATA CTGCTTGTGA GATCCTGTAT GAAAGAATGA 
     3421 AATCCCTGGG ACCTGATGTT CCAGAGTTAA TTATCCTCCC AGTGTACTCT GCTCTTCCCA 
     3481 GTGAGATGCA GACCCGAATC TTTGACCCAG CTCCACCAGG CAGCAGAAAG GTTGTGATTG 
     3541 CCACCAATAT CGCAGAGACA TCGCTGACTA TTGATGGTAT CTACTATGTG GTGGACCCAG 
     3601 GATTCGTGAA ACAGAAAGTT TACAATTCCA AGACAGGGAT TGACCAGCTC GTGGTGACGC 
     3661 CTATTTCTCA GGCTCAGGCA AAGCAACGAG CTGGCAGAGC TGGGAGAACA GGCCCAGGGA 
     3721 AGTGTTACAG GTTGTACACA GAACGTGCCT ACCGAGATGA AATGCTGACC ACCAACGTGC 
     3781 CGGAAATCCA GAGAACCAAC TTAGCAAGCA CAGTGCTGTC ACTCAAGGCC ATGGGTATCA 
     3841 ATGATCTGCT GTCCTTTGAT TTCATGGATG CCCCACCTAT GGAAACTTTG ATCACAGCCA 
     3901 TGGAGCAGCT GTACACACTG GGGGCCCTGG ATGACGAGGG CCTGCTCACT CGCTTGGGCC 
     3961 GCCGGATGGC AGAGTTCCCT CTGGAGCCAA TGCTATGCAA AATGCTCATC ATGTCTGTGC 
     4021 ATCTGGGCTG CAGTGAGGAA ATGCTGACCA TTGTATCCAT GCTGTCTGTG CAGAACGTCT 
     4081 TCTATAGGCC CAAGGATAAA CAAGCCCTTG CAGATCAGAA GAAGGCCAAA TTCCACCAGA 
     4141 CTGAAGGGGA CCACCTCACC CTGCTAGCTG TGTACAACTC CTGGAAGAAC AACAAGTTCT 
     4201 CCAACCCATG GTGCTATGAG AACTTTATCC AGGCTCGTTC CCTGCGCCGG GCCCAGGACA 
     4261 TTCGCGAGCA GATGTTAGGC ATAATGGACA GACACAAGCT GGATGTTGTT TCCTGTGGCA 
     4321 AGTCCACAGT CCGAGTGCAG AAGGCCATCT GCAGTGGGTT CTTCCGTAAT GCTGCCAAGA 
     4381 AAGACCCGCA GGAGGGTTAC CGGACACTGA TCGACCAGCA GGTGGTCTAT ATCCATCCTT 
     4441 CCAGTGCCCT CTTCAACAGA CAGCCAGAAT GGGTGGTGTA CCATGAGCTG GTGCTCACCA 
     4501 CCAAGGAATA CATGCGTGAA GTTACCACCA TCGACCCTCG GTGGCTTGTG GAGTTTGCCC 
     4561 CAGCCTTCTT CAAGGTCTCA GACCCAACTA AGCTAAGCAA ACAGAAGAAG CAACAGCGTC 
     4621 TTGAACCCTT GTACAACCGC TATGAGGAAC CCAATGCCTG GAGAATATCT CGAGCTTTCC 
     4681 GACGGCGCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4741 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4801 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4861 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4921 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4981 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5041 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5101 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5161 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5221 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5281 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5341 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5401 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5461 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5521 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5581 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5641 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5701 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5761 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5821 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5881 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5941 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6001 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6061 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6121 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6181 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6241 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6301 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6361 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6421 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6481 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6541 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6601 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6661 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6721 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6781 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6841 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6901 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6961 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7021 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7081 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7141 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7201 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7261 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7321 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7381 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7441 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7501 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7561 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7621 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7681 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7741 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7981 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8041 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8101 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8161 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8221 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8281 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8341 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8401 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8521 CAAAAATTTA ACGCGAATTT T
//