LOCUS       RC208384                7788 bp    DNA     circular  SYN 
DEFINITION  NFKB1 (Myc-DDK-tagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), transcript variant 1
ACCESSION   NM_003998
VERSION     NM_003998.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3945..4036 
                     /label="Myc-DDK"  
     misc_feature    4066..4651 
                     /label="PolyA signal"  
     misc_feature    4123..4142 
                     /label="Restriction Enzyme"  
     misc_feature    4927..5599 
                     /label="ColE1"  
     misc_feature    6029..6823 
                     /label="Kan/Neo"  
     misc_feature    6858..7160 
                     /label="SV40 ori"  
     misc_feature    7246..7723 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3927..3933 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3927 
                     /label="NFKB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAAGAT GATCCATATT TGGGAAGGCC TGAACAAATG TTTCATTTGG 
     1081 ATCCTTCTTT GACTCATACA ATATTTAATC CAGAAGTATT TCAACCACAG ATGGCACTGC 
     1141 CAACAGCAGA TGGCCCATAC CTTCAAATAT TAGAGCAACC TAAACAGAGA GGATTTCGTT 
     1201 TCCGTTATGT ATGTGAAGGC CCATCCCATG GTGGACTACC TGGTGCCTCT AGTGAAAAGA 
     1261 ACAAGAAGTC TTACCCTCAG GTCAAAATCT GCAACTATGT GGGACCAGCA AAGGTTATTG 
     1321 TTCAGTTGGT CACAAATGGA AAAAATATCC ACCTGCATGC CCACAGCCTG GTGGGAAAAC 
     1381 ACTGTGAGGA TGGGATCTGC ACTGTAACTG CTGGACCCAA GGACATGGTG GTCGGCTTCG 
     1441 CAAACCTGGG TATACTTCAT GTGACAAAGA AAAAAGTATT TGAAACACTG GAAGCACGAA 
     1501 TGACAGAGGC GTGTATAAGG GGCTATAATC CTGGACTCTT GGTGCACCCT GACCTTGCCT 
     1561 ATTTGCAAGC AGAAGGTGGA GGGGACCGGC AGCTGGGAGA TCGGGAAAAA GAGCTAATCC 
     1621 GCCAAGCAGC TCTGCAGCAG ACCAAGGAGA TGGACCTCAG CGTGGTGCGG CTCATGTTTA 
     1681 CAGCTTTTCT TCCGGATAGC ACTGGCAGCT TCACAAGGCG CCTGGAACCC GTGGTATCAG 
     1741 ACGCCATCTA TGACAGTAAA GCCCCCAATG CATCCAACTT GAAAATTGTA AGAATGGACA 
     1801 GGACAGCTGG ATGTGTGACT GGAGGGGAGG AAATTTATCT TCTTTGTGAC AAAGTTCAGA 
     1861 AAGATGACAT CCAGATTCGA TTTTATGAAG AGGAAGAAAA TGGTGGAGTC TGGGAAGGAT 
     1921 TTGGAGATTT TTCCCCCACA GATGTTCATA GACAATTTGC CATTGTCTTC AAAACTCCAA 
     1981 AGTATAAAGA TATTAATATT ACAAAACCAG CCTCTGTGTT TGTCCAGCTT CGGAGGAAAT 
     2041 CTGACTTGGA AACTAGTGAA CCAAAACCTT TCCTCTACTA TCCTGAAATC AAAGATAAAG 
     2101 AAGAAGTGCA GAGGAAACGT CAGAAGCTCA TGCCCAATTT TTCGGATAGT TTCGGCGGTG 
     2161 GTAGTGGTGC CGGAGCTGGA GGCGGAGGCA TGTTTGGTAG TGGCGGTGGA GGAGGGGGCA 
     2221 CTGGAAGTAC AGGTCCAGGG TATAGCTTCC CACACTATGG ATTTCCTACT TATGGTGGGA 
     2281 TTACTTTCCA TCCTGGAACT ACTAAATCTA ATGCTGGGAT GAAGCATGGA ACCATGGACA 
     2341 CTGAATCTAA AAAGGACCCT GAAGGTTGTG ACAAAAGTGA TGACAAAAAC ACTGTAAACC 
     2401 TCTTTGGGAA AGTTATTGAA ACCACAGAGC AAGATCAGGA GCCCAGCGAG GCCACCGTTG 
     2461 GGAATGGTGA GGTCACTCTA ACGTATGCAA CAGGAACAAA AGAAGAGAGT GCTGGAGTTC 
     2521 AGGATAACCT CTTTCTAGAG AAGGCTATGC AGCTTGCAAA GAGGCATGCC AATGCCCTTT 
     2581 TCGACTACGC GGTGACAGGA GACGTGAAGA TGCTGCTGGC CGTCCAGCGC CATCTCACTG 
     2641 CTGTGCAGGA TGAGAATGGG GACAGTGTCT TACACTTAGC AATCATCCAC CTTCATTCTC 
     2701 AACTTGTGAG GGATCTACTA GAAGTCACAT CTGGTTTGAT TTCTGATGAC ATTATCAACA 
     2761 TGAGAAATGA TCTGTACCAG ACGCCCTTGC ACTTGGCAGT GATCACTAAG CAGGAAGATG 
     2821 TGGTGGAGGA TTTGCTGAGG GCTGGGGCCG ACCTGAGCCT TCTGGACCGC TTGGGTAACT 
     2881 CTGTTTTGCA CCTAGCTGCC AAAGAAGGAC ATGATAAAGT TCTCAGTATC TTACTCAAGC 
     2941 ACAAAAAGGC AGCACTACTT CTTGACCACC CCAACGGGGA CGGTCTGAAT GCCATTCATC 
     3001 TAGCCATGAT GAGCAATAGC CTGCCATGTT TGCTGCTGCT GGTGGCCGCT GGGGCTGACG 
     3061 TCAATGCTCA GGAGCAGAAG TCCGGGCGCA CAGCACTGCA CCTGGCTGTG GAGCACGACA 
     3121 ACATCTCATT GGCAGGCTGC CTGCTCCTGG AGGGTGATGC CCATGTGGAC AGTACTACCT 
     3181 ACGATGGAAC CACACCCCTG CATATAGCAG CTGGGAGAGG GTCCACCAGG CTGGCAGCTC 
     3241 TTCTCAAAGC AGCAGGAGCA GATCCCCTGG TGGAGAACTT TGAGCCTCTC TATGACCTGG 
     3301 ATGACTCTTG GGAAAATGCA GGAGAGGATG AAGGAGTTGT GCCTGGAACC ACGCCTCTAG 
     3361 ATATGGCCAC CAGCTGGCAG GTATTTGACA TATTAAATGG GAAACCATAT GAGCCAGAGT 
     3421 TTACATCTGA TGATTTACTA GCACAAGGAG ACATGAAACA GCTGGCTGAA GATGTGAAGC 
     3481 TGCAGCTGTA TAAGTTACTA GAAATTCCTG ATCCAGACAA AAACTGGGCT ACTCTGGCGC 
     3541 AGAAATTAGG TCTGGGGATA CTTAATAATG CCTTCCGGCT GAGTCCTGCT CCTTCCAAAA 
     3601 CACTTATGGA CAACTATGAG GTCTCTGGGG GTACAGTCAG AGAGCTGGTG GAGGCCCTGA 
     3661 GACAAATGGG CTACACCGAA GCAATTGAAG TGATCCAGGC AGCCTCCAGC CCAGTGAAGA 
     3721 CCACCTCTCA GGCCCACTCG CTGCCTCTCT CGCCTGCCTC CACAAGGCAG CAAATAGACG 
     3781 AGCTCCGAGA CAGTGACAGT GTCTGCGACA GCGGCGTGGA GACATCCTTC CGCAAACTCA 
     3841 GCTTTACCGA GTCTCTGACC AGTGGTGCCT CACTGCTAAC TCTCAACAAA ATGCCCCATG 
     3901 ATTATGGGCA GGAAGGACCT CTAGAAGGCA AAATTACGCG TACGCGGCCG CTCGAGCAGA 
     3961 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4021 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4081 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4141 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4201 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4261 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4321 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4381 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4441 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4501 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4561 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4621 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4681 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4741 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4801 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4861 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4921 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4981 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5041 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5101 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5161 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5221 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5281 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5341 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5401 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5461 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5521 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5581 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5641 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5701 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5761 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5821 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5881 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5941 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6001 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6061 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6121 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6181 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6241 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6301 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6361 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6421 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6481 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6541 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6601 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6661 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6721 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6781 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6841 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6901 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6961 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7021 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7141 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7201 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7261 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7321 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7381 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7441 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7501 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7561 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7621 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7681 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7741 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//