LOCUS       RC208349L1                8853 bp    DNA     circular  SYN 
DEFINITION  AXIN1 (Myc-DDK-tagged)-Human axin 1 (AXIN1), transcript variant 2
ACCESSION   NM_181050
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7144..7324 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8052..8711 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7483..8051 
                     /label="ColE1"  
     misc_feature    6019..6039 
                     /label="LR50 primer"  
     misc_feature    5816..5887 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6028..6619 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5798..5804 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5798 
                     /label="AXIN1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAATATCCAA GAGCAGGGTT TCCCCTTGGA 
     3361 CCTCGGAGCA AGTTTCACCG AAGATGCTCC CCGACCCCCA GTGCCTGGTG AGGAGGGAGA 
     3421 ACTGGTGTCC ACAGACCCGA GGCCCGCCAG CTACAGTTTC TGCTCCGGGA AAGGTGTTGG 
     3481 CATTAAAGGT GAGACTTCGA CGGCCACTCC GAGGCGCTCG GATCTGGACC TGGGGTATGA 
     3541 GCCTGAGGGC AGTGCCTCCC CCACCCCACC ATACTTGAAG TGGGCTGAGT CACTGCATTC 
     3601 CCTGCTGGAT GACCAAGATG GGATAAGCCT GTTCAGGACT TTCCTGAAGC AGGAGGGCTG 
     3661 TGCCGACTTG CTGGACTTCT GGTTTGCCTG CACTGGCTTC AGGAAGCTGG AGCCCTGTGA 
     3721 CTCGAACGAG GAGAAGAGGC TGAAGCTGGC GAGAGCCATC TACCGAAAGT ACATTCTTGA 
     3781 TAACAATGGC ATCGTGTCCC GGCAGACCAA GCCAGCCACC AAGAGCTTCA TAAAGGGCTG 
     3841 CATCATGAAG CAGCTGATCG ATCCTGCCAT GTTTGACCAG GCCCAGACCG AAATCCAGGC 
     3901 CACTATGGAG GAAAACACCT ATCCCTCCTT CCTTAAGTCT GATATTTATT TGGAATATAC 
     3961 GAGGACAGGC TCGGAGAGCC CCAAAGTCTG TAGTGACCAG AGCTCTGGGT CAGGGACAGG 
     4021 GAAGGGCATA TCTGGATACC TGCCGACCTT AAATGAAGAT GAGGAATGGA AGTGTGACCA 
     4081 GGACATGGAT GAGGACGATG GCAGAGACGC TGCTCCCCCC GGAAGACTCC CTCAGAAGCT 
     4141 GCTCCTGGAG ACAGCTGCCC CGAGGGTCTC CTCCAGTAGA CGGTACAGCG AAGGCAGAGA 
     4201 GTTCAGGTAT GGATCCTGGC GGGAGCCAGT CAACCCCTAT TATGTCAATG CCGGCTATGC 
     4261 CCTGGCCCCA GCCACCAGTG CCAACGACAG CGAGCAGCAG AGCCTGTCCA GCGATGCAGA 
     4321 CACCCTGTCC CTCACGGACA GCAGCGTGGA TGGGATCCCC CCATACAGGA TCCGTAAGCA 
     4381 GCACCGCAGG GAGATGCAGG AGAGCGTGCA GGTCAATGGG CGGGTGCCCC TACCTCACAT 
     4441 TCCCCGCACG TACCGGGTGC CGAAGGAGGT CCGCGTGGAG CCTCAGAAGT TCGCGGAGGA 
     4501 GCTCATCCAC CGCCTGGAGG CTGTGCAGCG CACGCGGGAG GCCGAGGAGA AGCTGGAGGA 
     4561 GCGGCTGAAG CGCGTGCGCA TGGAGGAGGA AGGTGAGGAC GGCGATCCAT CGTCAGGGCC 
     4621 CCCAGGGCCG TGTCACAAGC TGCCTCCCGC CCCCGCTTGG CACCACTTCC CGCCCCGCTG 
     4681 TGTGGACATG GGCTGTGCCG GGCTCCGGGA TGCACACGAG GAGAACCCTG AGAGCATCCT 
     4741 GGACGAGCAC GTACAGCGTG TGCTGAGGAC ACCTGGCCGC CAGTCGCCTG GGCCTGGCCA 
     4801 TCGCTCCCCG GACAGTGGGC ACGTGGCCAA GATGCCAGTG GCACTGGGGG GTGCCGCCTC 
     4861 GGGGCACGGG AAGCACGTAC CCAAGTCAGG GGCGAAGCTG GACGCGGCCG GCCTGCACCA 
     4921 CCACCGACAC GTCCACCACC ACGTCCACCA CAGCACAGCC CGGCCCAAGG AGCAGGTGGA 
     4981 GGCCGAGGCC ACCCGCAGGG CCCAGAGCAG CTTCGCCTGG GGCCTGGAAC CACACAGCCA 
     5041 TGGGGCAAGG TCCCGAGGCT ACTCAGAGAG TGTTGGCGCT GCCCCCAACG CCAGTGATGG 
     5101 CCTCGCCCAC AGTGGGAAGG TGGGCGTGGC GTGCAAAAGA AATGCCAAGA AGGCTGAGTC 
     5161 GGGGAAGAGC GCCAGCACCG AGGTGCCAGG TGCCTCGGAG GATGCGGAGA AGAACCAGAA 
     5221 AATCATGCAG TGGATCATTG AGGGGGAAAA GGAGATCAGC AGGCACCGCA GGACCGGCCA 
     5281 CGGGTCTTCG GGGACGAGGA AGCCACAGCC CCATGAGAAC TCCAGACCCT TGTCCCTTGA 
     5341 GCACCCCTGG GCCGGCCCTC AGCTCCGGAC CTCCGTGCAG CCCTCCCACC TCTTCATCCA 
     5401 AGACCCCACC ATGCCACCCC ACCCAGCTCC CAACCCCCTA ACCCAGCTGG AGGAGGCGCG 
     5461 CCGACGTCTG GAGGAGGAAG AAAAGAGAGC CAGCCGAGCA CCCTCCAAGC AGAGGACAAG 
     5521 ATCGCAGAGG AAGGTGGGCG GCGGGAGTGC CCAGCCGTGT GACAGCATCG TTGTGGCGTA 
     5581 CTACTTCTGC GGGGAACCCA TCCCCTACCG CACCCTGGTG AGGGGCCGCG CTGTCACCCT 
     5641 GGGCCAGTTC AAGGAGCTGC TGACCAAAAA GGGCAGCTAC AGATACTACT TCAAGAAAGT 
     5701 GAGCGACGAG TTTGACTGTG GGGTGGTGTT TGAGGAGGTT CGAGAGGACG AGGCCGTCCT 
     5761 GCCCGTCTTT GAGGAGAAGA TCATCGGCAA AGTGGAGAAG GTGGACACGC GTACGCGGCC 
     5821 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     5881 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     5941 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6001 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6061 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6121 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6181 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6241 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6301 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6361 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6421 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6481 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     6541 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     6601 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     6661 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     6721 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     6781 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     6841 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     6901 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     6961 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7021 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7081 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7141 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7201 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7261 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7321 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7381 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7441 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7501 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     7561 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     7621 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     7681 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     7741 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     7801 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     7861 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     7921 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     7981 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8041 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8101 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8161 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8221 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8281 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8341 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8401 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8461 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     8521 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     8581 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     8641 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     8701 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8761 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTC 
     8821 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//