LOCUS       RC208343                6984 bp    DNA     circular  SYN 
DEFINITION  ALOX12B (Myc-DDK-tagged)-Human arachidonate 12-lipoxygenase, 12R type (ALOX12B)
ACCESSION   NM_001139
VERSION     NM_001139.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3141..3232 
                     /label="Myc-DDK"  
     misc_feature    3262..3847 
                     /label="PolyA signal"  
     misc_feature    3319..3338 
                     /label="Restriction Enzyme"  
     misc_feature    4123..4795 
                     /label="ColE1"  
     misc_feature    5225..6019 
                     /label="Kan/Neo"  
     misc_feature    6054..6356 
                     /label="SV40 ori"  
     misc_feature    6442..6919 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3123..3129 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3123 
                     /label="ALOX12B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCTAC AAAGTCAGGG TGGCCACAGG CACCGACCTC TTGTCGGGAA 
     1081 CACGGGACTC CATCTCACTG ACCATTGTGG GGACACAAGG AGAGAGCCAT AAGCAGCTGC 
     1141 TGAACCACTT TGGGAGAGAC TTTGCAACTG GGGCGGTGGG CCAGTACACC GTGCAGTGCC 
     1201 CTCAGGACCT GGGTGAGCTC ATCATCATCC GCCTGCACAA AGAGCGGTAC GCCTTCTTCC 
     1261 CCAAGGACCC TTGGTACTGC AACTATGTGC AGATCTGTGC CCCCAACGGC CGTATCTACC 
     1321 ACTTCCCCGC CTACCAGTGG ATGGATGGCT ACGAGACCCT GGCACTCCGG GAGGCCACAG 
     1381 GAAAGACAAC AGCAGATGAC TCGCTCCCCG TCCTCCTGGA GCACAGAAAA GAGGAGATCA 
     1441 GAGCCAAGCA GGACTTCTAC CACTGGCGAG TCTTTCTTCC TGGCCTGCCC AGCTATGTGC 
     1501 ACATTCCCAG TTACCGCCCT CCGGTGCGGA GGCATCGCAA CCCCAACCGG CCTGAGTGGA 
     1561 ATGGCTATAT TCCGGGATTC CCAATTCTCA TCAACTTTAA GGCCACCAAG TTCCTGAACT 
     1621 TAAATCTCCG CTACTCCTTC CTCAAGACGG CCTCCTTCTT CGTCCGCCTG GGGCCCATGG 
     1681 CACTGGCTTT CAAAGTCCGC GGCCTGTTGG ACTGCAAACA TTCGTGGAAG AGGCTGAAGG 
     1741 ACATTAGGAA AATTTTCCCT GGCAAGAAAT CTGTCGTCTC CGAGTACGTG GCCGAGCACT 
     1801 GGGCAGAGGA CACCTTCTTT GGGTACCAGT ACCTCAACGG CGTCAACCCC GGCCTGATCC 
     1861 GCCGCTGCAC GCGGATCCCA GACAAGTTCC CCGTCACAGA CGACATGGTG GCTCCGTTCC 
     1921 TGGGCGAGGG AACGTGCTTG CAAGCGGAGC TGGAGAAGGG GAACATTTAC CTGGCCGACT 
     1981 ACCGCATCAT GGAGGGCATC CCCACCGTGG AGCTCAGCGG CCGGAAGCAG CACCACTGCG 
     2041 CCCCCCTCTG CCTGCTGCAC TTTGGACCCG AGGGCAAGAT GATGCCCATC GCCATCCAGC 
     2101 TCAGCCAGAC CCCTGGGCCA GATTGCCCCA TCTTCCTGCC CAGTGATTCT GAGTGGGACT 
     2161 GGCTGCTAGC CAAGACGTGG GTACGCTATG CGGAGTTCTA CAGCCACGAG GCCATCGCCC 
     2221 ACCTGCTGGA GACACACCTC ATTGCTGAGG CCTTCTGCCT GGCCTTGCTG AGGAACCTGC 
     2281 CCATGTGCCA CCCCCTCTAC AAGCTCCTCA TCCCCCATAC CCGATACACC GTCCAGATCA 
     2341 ACAGCATTGG CCGGGCCGTT CTCCTCAATG AGGGGGGGCT CTCTGCCAAG GGCATGTCCC 
     2401 TGGGCGTGGA AGGCTTTGCT GGGGTGATGG TACGGGCTCT GTCGGAGCTC ACCTATGACA 
     2461 GCCTCTACCT CCCCAATGAC TTTGTGGAGC GTGGGGTCCA GGACCTGCCT GGATATTACT 
     2521 ACCGCGATGA CAGCTTGGCG GTGTGGAATG CACTGGAGAA GTATGTGACG GAGATCATCA 
     2581 CCTATTATTA CCCGAGTGAC GCAGCCGTGG AGGGTGATCC GGAATTGCAG TCTTGGGTGC 
     2641 AGGAAATATT TAAAGAGTGC CTCCTGGGGC GGGAGAGCTC AGGCTTCCCT AGGTGCTTGC 
     2701 GAACCGTGCC TGAGCTGATC CGATATGTCA CTATAGTCAT CTACACCTGC TCTGCCAAGC 
     2761 ACGCTGCTGT CAACACAGGC CAGATGGAGT TCACCGCCTG GATGCCCAAC TTCCCAGCGT 
     2821 CCATGCGGAA TCCACCGATT CAGACTAAGG GGCTGACCAC TCTGGAGACC TTCATGGACA 
     2881 CGTTGCCGGA TGTGAAGACC ACGTGCATCA CGCTGCTGGT GCTCTGGACC CTCAGCCGAG 
     2941 AGCCTGACGA CAGGCGGCCC CTGGGACACT TCCCGGACAT TCACTTCGTG GAGGAGGCCC 
     3001 CGCGGAGGAG CATAGAGGCG TTCCGCCAGC GCCTGAACCA GATCTCACAC GACATCCGCC 
     3061 AGCGCAACAA GTGCCTTCCC ATCCCCTACT ACTACCTGGA CCCGGTGCTG ATTGAGAACA 
     3121 GCATTTCTAT TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3181 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3241 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3301 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3361 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3421 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3481 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3541 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3601 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3661 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3721 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3781 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3841 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3901 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3961 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4021 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4081 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4141 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4201 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4261 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4321 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4381 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4441 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4501 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4561 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4621 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4681 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4741 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4801 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4861 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4921 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4981 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5041 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5101 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5161 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5221 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5281 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5341 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5401 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5461 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5521 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5581 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5641 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5701 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5761 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5821 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5881 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5941 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6001 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6061 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6121 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6181 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6241 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6301 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6361 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6421 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6481 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6541 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6601 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6661 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6721 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6781 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6841 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6901 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6961 TAACAAAAAT TTAACGCGAA TTTT
//