LOCUS       RC208264                9306 bp    DNA     circular  SYN 
DEFINITION  NUP153 (Myc-DDK-tagged)-Human nucleoporin 153kDa (NUP153)
ACCESSION   NM_005124
VERSION     NM_005124.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5463..5554 
                     /label="Myc-DDK"  
     misc_feature    5584..6169 
                     /label="PolyA signal"  
     misc_feature    5641..5660 
                     /label="Restriction Enzyme"  
     misc_feature    6445..7117 
                     /label="ColE1"  
     misc_feature    7547..8341 
                     /label="Kan/Neo"  
     misc_feature    8376..8678 
                     /label="SV40 ori"  
     misc_feature    8764..9241 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5445..5451 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5445 
                     /label="NUP153"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCGGGA GCCGGAGGAG TCGGAGGGGG CGGTGGCGGC AAGATCCGGA 
     1081 CGCGGCGTTG CCACCAGGGG CCAATTAAGC CTTACCAGCA GGGGCGACAA CAGCATCAGG 
     1141 GCATTCTTAG CAGGGTTACA GAATCTGTTA AGAATATTGT GCCAGGGTGG CTACAAAGAT 
     1201 ACTTCAACAA GAATGAAGAT GTATGCAGCT GTTCAACAGA CACAAGCGAG GTTCCACGCT 
     1261 GGCCAGAAAA TAAAGAGGAC CATCTGGTAT ATGCCGATGA GGAGAGCTCT AATATTACTG 
     1321 ATGGGAGAAT CACACCTGAG CCAGCAGTCA GTAATACAGA AGAACCTTCA ACAACTAGTA 
     1381 CTGCTTCAAA TTATCCAGAT GTGTTAACAA GGCCTTCTCT TCATCGGAGC CATCTGAATT 
     1441 TTTCCATGTT GGAATCCCCT GCATTACACT GTCAGCCATC TACATCCTCG GCATTCCCAA 
     1501 TTGGCAGTTC GGGATTTTCC CTTGTAAAGG AAATTAAAGA TTCTACCTCT CAGCATGATG 
     1561 ATGATAACAT CTCAACTACC AGTGGTTTTT CTTCAAGAGC TTCTGATAAA GATATAACTG 
     1621 TTTCAAAGAA CACTTCATTG CCACCTCTGT GGTCCCCAGA AGCTGAACGT TCTCACTCAC 
     1681 TCTCACAGCA CACTGCCACC AGCTCAAAAA AACCAGCATT CAACTTGTCT GCCTTTGGAA 
     1741 CACTTTCCCC TTCACTTGGG AATTCTTCAA TCCTTAAAAC CAGTCAGCTT GGAGATTCTC 
     1801 CTTTTTATCC TGGAAAAACA ACATACGGTG GGGCAGCAGC TGCTGTAAGA CAGTCTAAAC 
     1861 TACGAAATAC ACCTTATCAG GCACCAGTTA GAAGACAAAT GAAAGCTAAG CAACTCAGTG 
     1921 CACAATCTTA CGGTGTGACC AGTTCAACAG CTCGGCGAAT ATTGCAGTCT TTAGAGAAGA 
     1981 TGTCAAGCCC TTTAGCGGAT GCAAAAAGAA TTCCATCCAT TGTTTCTTCT CCTCTGAATT 
     2041 CTCCTCTTGA TAGGAGTGGG ATAGATATCA CAGATTTTCA GGCCAAAAGA GAAAAGGTGG 
     2101 ATTCTCAATA TCCTCCTGTT CAGAGACTTA TGACCCCAAA GCCAGTTTCC ATAGCAACAA 
     2161 ATCGAAGTGT TTATTTTAAA CCATCTCTGA CTCCTTCTGG TGAATTCAGG AAGACTAATC 
     2221 AAAGAATAGA TAACAAGTGC AGTACTGGAT ATGAAAAAAA TATGACACCC GGACAAAATA 
     2281 GAGAACAACG AGAAAGTGGC TTTTCATATC CAAATTTCAG TTTGCCTGCA GCCAATGGTT 
     2341 TATCTTCTGG AGTAGGTGGT GGAGGTGGCA AGATGAGACG AGAAAGAACA CGCTTTGTTG 
     2401 CTTCTAAACC TCTGGAGGAG GAGGAAATGG AAGTTCCAGT ATTACCGAAA ATCTCTCTAC 
     2461 CGATCACCAG TTCTTCACTG CCTACCTTTA ATTTTAGTTC CCCTGAGATC ACAACTTCCT 
     2521 CTCCATCACC CATCAATTCG TCTCAAGCAT TAACAAACAA GGTACAAATG ACCTCTCCGA 
     2581 GCAGCACTGG CAGTCCCATG TTTAAATTTT CATCTCCAAT CGTAAAATCT ACTGAGGCAA 
     2641 ATGTACTACC TCCATCATCT ATTGGATTTA CATTTAGTGT GCCTGTTGCA AAAACAGCAG 
     2701 AACTTTCTGG TTCTAGTAGT ACTTTAGAAC CAATTATAAG TAGTTCAGCT CATCATGTCA 
     2761 CTACAGTGAA CAGTACAAAT TGTAAGAAGA CACCACCTGA AGATTGTGAG GGTCCTTTTA 
     2821 GACCTGCAGA AATCCTGAAA GAAGGAAGTG TTCTAGATAT TCTGAAAAGC CCTGGTTTCG 
     2881 CATCGCCGAA GATAGATTCT GTTGCTGCTC AGCCCACCGC AACAAGCCCA GTAGTTTATA 
     2941 CAAGACCAGC AATAAGTAGC TTTTCTTCTA GTGGAATTGG GTTTGGGGAG AGTTTAAAAG 
     3001 CTGGGTCATC ATGGCAGTGT GATACATGTC TACTCCAGAA CAAAGTTACA GACAACAAAT 
     3061 GCATAGCCTG TCAAGCAGCA AAATTGTCAC CCAGAGATAC TGCTAAACAG ACTGGAATTG 
     3121 AAACACCAAA TAAAAGTGGC AAAACAACTC TTTCTGCATC AGGGACAGGC TTTGGAGACA 
     3181 AATTTAAACC AGTGATAGGC ACTTGGGATT GTGATACCTG TTTAGTGCAA AATAAACCTG 
     3241 AAGCAATAAA ATGTGTAGCC TGTGAAACAC CGAAACCTGG AACTTGTGTG AAGCGAGCCC 
     3301 TTACATTGAC AGTGGTTTCG GAAAGTGCTG AGACTATGAC TGCTTCATCT TCCAGCTGCA 
     3361 CTGTAACCAC TGGTACCTTA GGATTTGGAG ATAAATTCAA AAGGCCCATT GGATCTTGGG 
     3421 AGTGTTCAGT ATGCTGTGTT TCTAATAATG CAGAAGACAA TAAGTGTGTG TCCTGTATGT 
     3481 CTGAGAAACC AGGAAGTTCA GTACCTGCTT CAAGTAGCAG CACTGTACCT GTCTCTCTGC 
     3541 CTTCTGGAGG CTCTCTAGGA TTGGAAAAGT TCAAGAAACC CGAGGGAAGC TGGGACTGTG 
     3601 AATTGTGCCT AGTGCAGAAT AAGGCAGACT CTACCAAATG TTTGGCATGT GAAAGTGCAA 
     3661 AGCCAGGCAC AAAATCTGGG TTTAAAGGCT TTGACACATC TTCCTCATCT TCGAACTCAG 
     3721 CAGCCTCCTC ATCCTTCAAA TTTGGTGTCT CATCATCCTC TTCTGGGCCT TCTCAGACTT 
     3781 TAACAAGCAC TGGAAATTTT AAATTTGGAG ATCAGGGAGG ATTCAAAATA GGTGTGTCAT 
     3841 CTGATTCTGG GTCTATAAAC CCCATGAGTG AAGGCTTTAA ATTTTCTAAA CCAATAGGAG 
     3901 ATTTTAAATT TGGAGTTTCA TCTGAATCTA AGCCCGAAGA AGTTAAAAAA GATAGTAAGA 
     3961 ATGATAATTT TAAGTTTGGA CTTTCTTCTG GTTTAAGCAA CCCAGTTTCT TTAACTCCAT 
     4021 TTCAATTTGG GGTATCTAAT CTTGGACAGG AAGAAAAGAA AGAGGAACTG CCCAAATCTT 
     4081 CCTCTGCAGG TTTTAGCTTT GGTACAGGTG TTATTAACTC CACCCCTGCT CCTGCTAACA 
     4141 CCATAGTGAC CTCTGAGAAC AAGAGCAGCT TCAACCTTGG AACCATAGAA ACCAAGAGTG 
     4201 CTTCAGTGGC TCCTTTCACA TGTAAGACAT CAGAAGCTAA AAAAGAAGAA ATGCCTGCCA 
     4261 CCAAAGGAGG ATTCTCTTTT GGCAACGTGG AGCCTGCCTC TCTGCCATCT GCCTCAGTGT 
     4321 TTGTTTTGGG AAGGACAGAA GAGAAACAGC AAGAGCCTGT CACTTCTACT TCCCTAGTTT 
     4381 TTGGGAAGAA AGCTGACAAT GAAGAGCCAA AGTGTCAACC AGTGTTTTCC TTTGGGAATT 
     4441 CAGAGCAAAC CAAAGATGAG AATTCTTCAA AGTCCACATT TAGTTTTAGT ATGACAAAAC 
     4501 CATCTGAGAA GGAATCTGAA CAGCCAGCAA AAGCCACTTT TGCCTTTGGA GCTCAAACTA 
     4561 GTACTACAGC TGATCAAGGT GCAGCAAAGC CAGTTTTTAG TTTCTTGAAC AACAGTTCCT 
     4621 CTAGTTCAAG TACACCAGCC ACTTCTGCTG GTGGTGGCAT ATTTGGTAGT TCCACCTCTT 
     4681 CCTCCAATCC ACCTGTGGCT ACCTTTGTGT TTGGACAGTC CAGCAATCCT GTGAGCAGCT 
     4741 CTGCCTTTGG TAACACTGCT GAATCCAGCA CCTCTCAGTC TTTGCTATTT TCTCAAGATA 
     4801 GCAAACTAGC AACCACATCC AGCACAGGTA CAGCTGTCAC CCCATTTGTC TTTGGTCCAG 
     4861 GAGCCAGCAG TAATAATACT ACCACCTCTG GTTTCGGCTT TGGAGCCACA ACCACATCTA 
     4921 GCTCTGCAGG ATCCTCCTTT GTATTTGGAA CTGGACCCTC AGCACCATCT GCCAGTCCAG 
     4981 CATTTGGTGC TAACCAGACC CCAACATTTG GACAAAGTCA AGGTGCCAGC CAGCCCAATC 
     5041 CCCCAGGCTT TGGATCTATA TCATCTTCCA CAGCATTATT TCCCACTGGT TCTCAGCCTG 
     5101 CACCACCTAC TTTTGGGACA GTGTCAAGCA GTAGCCAGCC CCCTGTGTTT GGACAGCAAC 
     5161 CTAGTCAGTC TGCATTTGGC TCTGGAACAA CTCCTAATTC TAGTTCGGCT TTCCAGTTTG 
     5221 GCAGCAGCAC TACAAATTTC AACTTCACAA ACAACAGTCC ATCAGGAGTG TTCACATTTG 
     5281 GTGCAAATTC TAGCACACCT GCAGCCTCAG CCCAGCCTTC AGGCTCGGGG GGCTTTCCAT 
     5341 TTAACCAGTC TCCAGCAGCA TTTACAGTGG GGTCAAATGG GAAAAATGTG TTCTCTTCTT 
     5401 CTGGAACTTC ATTCTCTGGT CGCAAGATAA AGACTGCTGT TAGACGCAGG AAAACGCGTA 
     5461 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5521 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5581 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5641 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5701 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5761 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5821 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5881 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5941 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     6001 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     6061 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6121 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6181 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6241 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6301 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6361 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6421 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6481 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6541 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6601 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6661 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6721 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6781 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6841 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6901 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6961 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     7021 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     7081 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7141 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7201 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7261 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7321 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7381 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7441 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7501 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7561 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7621 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7681 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7741 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7801 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7861 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7921 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7981 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     8041 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     8101 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8161 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8221 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8281 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8341 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8401 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8461 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8521 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8581 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8641 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8701 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8761 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8821 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8881 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8941 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     9001 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     9061 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9121 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9181 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9241 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9301 AATTTT
//