LOCUS       RC208239L2                9297 bp    DNA     circular  SYN 
DEFINITION  GFM1 (Myc-DDK-tagged)-Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein
ACCESSION   NM_024996
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7586..7766 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8494..9153 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7814..8433 
                     /label="ColE1"  
     misc_feature    6461..6481 
                     /label="LR50"  
     misc_feature    5601..6326 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6470..7061 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5573..5580 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5573 
                     /label="GFM1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGACTCCTG GGAGCTGCAG CCGTCGCGGC 
     3361 TCTGGGGCGC GGAAGGGCCC CCGCCTCCCT AGGCTGGCAG AGGAAGCAGG TTAATTGGAA 
     3421 GGCCTGCCGA TGGTCTTCAT CAGGGGTGAT TCCTAATGAA AAAATACGAA ATATTGGAAT 
     3481 CTCAGCTCAC ATTGATTCTG GGAAAACTAC ATTAACAGAA CGAGTCCTTT ACTACACTGG 
     3541 CAGAATTGCA AAGATGCATG AGGTGAAAGG TAAAGATGGA GTTGGTGCTG TCATGGATTC 
     3601 CATGGAACTA GAGAGACAAA GAGGAATCAC TATTCAGTCA GCAGCCACTT ACACCATGTG 
     3661 GAAAGATGTC AATATTAACA TTATAGATAC TCCTGGGCAT GTGGACTTCA CAATAGAAGT 
     3721 GGAAAGGGCC CTGAGAGTGT TGGATGGTGC AGTCCTTGTT CTCTGTGCTG TTGGAGGGGT 
     3781 ACAGTGCCAG ACCATGACTG TCAATCGTCA GATGAAGCGC TACAACGTTC CGTTTCTAAC 
     3841 TTTTATTAAC AAATTGGACC GAATGGGCTC CAACCCAGCC AGGGCCCTGC AGCAAATGAG 
     3901 GTCTAAACTA AATCATAATG CAGCGTTTAT GCAGATACCC ATGGGTTTGG AGGGTAATTT 
     3961 TAAAGGTATT GTAGATCTTA TTGAGGAACG AGCCATCTAT TTTGATGGAG ACTTTGGTCA 
     4021 GATTGTTCGA TATGGTGAGA TTCCAGCTGA ATTAAGGGCG GCGGCCACTG ACCACCGGCA 
     4081 GGAGCTAATT GAATGTGTTG CCAATTCAGA TGAACAGCTT GGTGAGATGT TTCTGGAAGA 
     4141 AAAAATCCCC TCGATTTCTG ATTTAAAGCT AGCAATTCGA AGAGCTACTC TGAAAAGATC 
     4201 ATTTACTCCT GTATTTTTGG GAAGCGCCTT GAAGAACAAA GGAGTTCAGC CTCTTTTAGA 
     4261 TGCTGTTTTA GAATACCTCC CAAATCCATC TGAAGTCCAG AACTATGCTA TTCTCAATAA 
     4321 AGAGGATGAC TCAAAAGAGA AAACCAAAAT CCTAATGAAC TCCAGTAGAG ACAATTCCCA 
     4381 CCCATTTGTA GGCCTGGCTT TTAAACTGGA GGTAGGTCGA TTTGGACAAT TAACTTATGT 
     4441 TCGCAGTTAT CAGGGAGAGC TAAAGAAGGG TGACACCATC TATAACACAA GGACAAGAAA 
     4501 GAAAGTACGG TTGCAACGGC TGGCTCGCAT GCATGCCGAC ATGATGGAGG ATGTTGAGGA 
     4561 AGTATATGCC GGAGACATCT GTGCATTGTT TGGCATTGAC TGTGCTAGTG GAGACACATT 
     4621 CACAGACAAA GCCAACAGCG GCCTTTCTAT GGAGTCAATT CATGTTCCTG ATCCTGTCAT 
     4681 TTCAATAGCA ATGAAGCCTT CTAACAAGAA CGATCTGGAA AAATTTTCAA AAGGTATTGG 
     4741 CAGGTTTACA AGAGAAGATC CCACATTTAA AGTATACTTT GACACTGAGA ACAAAGAGAC 
     4801 AGTTATATCT GGAATGGGAG AATTACACCT GGAAATCTAT GCTCAGAGGC TGGAAAGAGA 
     4861 GTATGGCTGT CCTTGTATCA CAGGAAAGCC AAAAGTTGCC TTTCGAGAGA CCATTACTGC 
     4921 CCCTGTCCCG TTTGACTTTA CACATAAAAA ACAATCAGGT GGTGCAGGCC AGTATGGAAA 
     4981 AGTAATAGGT GTCCTGGAGC CTCTGGACCC AGAGGACTAC ACTAAATTGG AATTTTCAGA 
     5041 TGAAACATTC GGATCAAATA TTCCAAAGCA GTTTGTGCCT GCTGTAGAAA AGGGGTTTTT 
     5101 AGATGCCTGC GAGAAGGGCC CTCTTTCTGG TCACAAGCTC TCTGGGCTCC GGTTTGTCCT 
     5161 GCAAGATGGA GCACACCACA TGGTTGATTC TAATGAAATC TCTTTCATCC GAGCAGGAGA 
     5221 AGGTGCTCTT AAACAAGCCT TGGCAAATGC AACATTATGT ATTCTTGAAC CTATTATGGC 
     5281 TGTGGAAGTT GTAGCTCCAA ATGAATTTCA GGGACAAGTA ATTGCAGGAA TTAACCGACG 
     5341 CCATGGGGTA ATCACTGGGC AAGATGGAGT TGAGGACTAT TTTACACTGT ATGCAGATGT 
     5401 CCCTCTAAAT GATATGTTTG GTTATTCCAC TGAACTTAGG TCATGCACAG AGGGAAAGGG 
     5461 AGAATACACA ATGGAGTATA GCAGGTATCA GCCATGTTTA CCATCCACAC AAGAAGACGT 
     5521 CATTAATAAG TATTTGGAAG CTACAGGTCA ACTTCCTGTT AAAAAAGGAA AAGCCAAGAA 
     5581 CAGCGGACCG ACGCGTACGC GGCCGCTCGA GATGAGCGGG GGCGAGGAGC TGTTCGCCGG 
     5641 CATCGTGCCC GTGCTGATCG AGCTGGACGG CGACGTGCAC GGCCACAAGT TCAGCGTGCG 
     5701 CGGCGAGGGC GAGGGCGACG CCGACTACGG CAAGCTGGAG ATCAAGTTCA TCTGCACCAC 
     5761 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT GGTGACCACC CTCTGCTACG GCATCCAGTG 
     5821 CTTCGCCCGC TACCCCGAGC ACATGAAGAT GAACGACTTC TTCAAGAGCG CCATGCCCGA 
     5881 GGGCTACATC CAGGAGCGCA CCATCCAGTT CCAGGACGAC GGCAAGTACA AGACCCGCGG 
     5941 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCAAGGACTT 
     6001 CAAGGAGGAC GGCAACATCC TGGGCCACAA GCTGGAGTAC AGCTTCAACA GCCACAACGT 
     6061 GTACATCCGC CCCGACAAGG CCAACAACGG CCTGGAGGCT AACTTCAAGA CCCGCCACAA 
     6121 CATCGAGGGC GGCGGCGTGC AGCTGGCCGA CCACTACCAG ACCAACGTGC CCCTGGGCGA 
     6181 CGGCCCCGTG CTGATCCCCA TCAACCACTA CCTGAGCACT CAGACCAAGA TCAGCAAGGA 
     6241 CCGCAACGAG GCCCGCGACC ACATGGTGCT CCTGGAGTCC TTCAGCGCCT GCTGCCACAC 
     6301 CCACGGCATG GACGAGCTGT ACAGGTCCGG ACTCAGATAA GTTTAAACGG CCGGCCGCGG 
     6361 TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA 
     6421 GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT TCGATATCAA GCTTATCGAT 
     6481 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 
     6541 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 
     6601 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 
     6661 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 
     6721 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 
     6781 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 
     6841 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 
     6901 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 
     6961 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 
     7021 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 
     7081 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC 
     7141 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC 
     7201 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 
     7261 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA 
     7321 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC 
     7381 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA 
     7441 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA 
     7501 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT 
     7561 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT 
     7621 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 
     7681 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 
     7741 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA 
     7801 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     7861 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     7921 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     7981 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     8041 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     8101 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     8161 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     8221 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     8281 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     8341 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     8401 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     8461 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGATTACGC CCCGCCCTGC 
     8521 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 
     8581 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 
     8641 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA 
     8701 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA 
     8761 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC 
     8821 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG 
     8881 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC 
     8941 ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA 
     9001 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC 
     9061 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT 
     9121 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATACTCTT CCTTTTTCAA 
     9181 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     9241 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAC
//