LOCUS       RC208141                6963 bp    DNA     circular  SYN 
DEFINITION  GAS2L3 (Myc-DDK-tagged)-Human growth arrest-specific 2 like 3 (GAS2L3)
ACCESSION   NM_174942
VERSION     NM_174942.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3120..3211 
                     /label="Myc-DDK"  
     misc_feature    3241..3826 
                     /label="PolyA signal"  
     misc_feature    3298..3317 
                     /label="Restriction Enzyme"  
     misc_feature    4102..4774 
                     /label="ColE1"  
     misc_feature    5204..5998 
                     /label="Kan/Neo"  
     misc_feature    6033..6335 
                     /label="SV40 ori"  
     misc_feature    6421..6898 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3102..3108 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3102 
                     /label="GAS2L3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCCTGCA ATTCAAGTAT GGTTTGGAGA AGATCTGCCT CTAAGTCCTC 
     1081 GGAGTCCTCT GACTCCCAGA CACGGACCAG GATTGGCTAA TGTTTGTCAG TACGATGAGT 
     1141 GGATAGCTGT GAGGCATGAA GCCACTTTGT TGCCCATGCA AGAAGATCTG TCAATCTGGT 
     1201 TATCTGGTTT ATTAGGTATT AAAGTTAAGG CAGAAAAATT ATTGGAAGAA CTTGATAATG 
     1261 GAGTACTATT ATGTCAACTG ATTGATGTTC TTCAAAACAT GGTGAAAACA TGCAACTCTG 
     1321 AAGAATCAGG GAATTTTCCA ATGAGAAAAG TGCCCTGTAA GAAAGATGCT GCATCAGGTT 
     1381 CATTCTTTGC TCGGGACAAT ACCGCAAACT TCCTTCACTG GTGTAGGGAC ATTGGGGTTG 
     1441 ATGAAACTTA CCTCTTTGAA TCTGAAGGTT TAGTTTTGCA CAAAGATCCA AGACAGGTGT 
     1501 ATCTTTGTCT TCTTGAAATT GGTCGAATTG TGTCAAGATA CGGGGTTGAG CCACCAGTGT 
     1561 TAGTAAAACT TGAGAAAGAA ATTGAGTTAG AAGAGACTTT GCTTAATACT TCTGGGCCTG 
     1621 AAGATTCCAT CAGCATTCCA AAATCATGCT GTCGGCATGA AGAGCTACAT GAAGCTGTTA 
     1681 AACATATTGC TGAGGACCCT CCTTGTAGTT GTTCTCATCG ATTTTCTATT GAGTATTTAT 
     1741 CTGAAGGACG GTACCGACTA GGGGATAAAA TACTCTTTAT AAGAATGCTT CATGGAAAAC 
     1801 ATGTCATGGT TCGCGTTGGT GGAGGCTGGG ATACTCTTCA AGGATTTTTG CTTAAATATG 
     1861 ACCCCTGTCG AATATTACAG TTTGCCACAC TAGAACAAAA AATTTTAGCA TTTCAAAAAG 
     1921 GAGTTTCTAA TGAAAGTGTA CCTGATTCGC CTGCCAGAAC ACCTCAGCCT CCTGAAATGA 
     1981 ATCCTTTGTC AGCAGTTAAC ATGTTTCAGA AACAAAATTC AAAACCCAGC GTGCCAGTTA 
     2041 GTATTCCAAA AAGCAAAGAA AAACAGGGAC GTCCACCAGG TGCATTGGTG CCAGCATCTT 
     2101 CACTGAAAGG AGGTAATCTG GGCTCTATGT CAGTCCGTTC TAAATTGCCA AATTCTCCAG 
     2161 CAGCATCTTC TCATCCCAAG CTCAAGTCTT CAAAAGGCAT AACGAAGAAA CCGCAGGCTC 
     2221 CTTCAAACAA TGCATCATCT TCACTTGCTT CATTAAATCC AGTAGGTAAA AACACTTCTT 
     2281 CACCAGCTTT ACCAAGAACT GCACCTTGTA TATCTGAGTC ACCGAGAAAA TGTATTTCAT 
     2341 CCCCCAATAC CCCCAAGGCC AAGGTTATTC CAGCCCAGAA TTCAGCAGAT CTGCCCGAGT 
     2401 CCACACTTTT GCCAAATAAG TGTTCAGGAA AAACTCAACC TAAGTATTTG AAACATAATC 
     2461 ATATTTCTTC CAGAGATAAT GCAGTATCTC ACTTAGCTGC ACATTCAAAT TCATCCTCAA 
     2521 AATGTCCCAA GCTGCCTAAA GCAAATATAC CTGTAAGACC TAAACCTTCT TTCCAGTCCT 
     2581 CTGCAAAAAT GACAAAAACC AGTTCCAAAA CCATAGCCAC GGGTCTAGGA ACACAGTCTC 
     2641 AACCATCCGA TGGAGCCCCA CAAGCAAAGC CAGTCCCAGC ACAGAAACTT AAATCGGCCT 
     2701 TGAATTTAAA TCAGCCAGTT TCTGTGTCCT CAGTTTCTCC TGTAAAAGCC ACACAGAAAT 
     2761 CAAAAGATAA GAATATAGTT TCAGCTACCA AAAAGCAGCC TCAGAATAAA AGTGCATTTC 
     2821 AGAAGACAGG ACCCAGCTCC TTGAAGTCTC CTGGCCGTAC CCCACTGTCC ATCGTGAGCC 
     2881 TACCCCAGTC TTCTACCAAA ACACAAACTG CACCGAAGTC AGCACAGACT GTCGCTAAGA 
     2941 GCCAGCATTC AACTAAAGGG CCTCCCAGAA GTGGCAAAAC CCCAGCTTCA ATCAGGAAAC 
     3001 CACCCTCATC TGTTAAGGAT GCAGATAGTG GAGATAAAAA ACCTACTGCA AAGAAAAAGG 
     3061 AAGATGATGA CCATTATTTT GTCATGACTG GAAGTAAGAA ACCTAGAAAA ACGCGTACGC 
     3121 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3181 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3241 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3301 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3361 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3421 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3481 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3541 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3601 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     3661 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     3721 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     3781 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     3841 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     3901 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     3961 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4021 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4081 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4141 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4201 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4261 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4321 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4381 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4441 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4501 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4561 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4621 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     4681 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     4741 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     4801 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     4861 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     4921 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     4981 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5041 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5101 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5161 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5221 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5281 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5341 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5401 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5461 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5521 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5581 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     5641 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     5701 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     5761 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     5821 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     5881 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     5941 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6001 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6061 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6121 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6181 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6241 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6301 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6361 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6421 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6481 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6541 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6601 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     6661 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     6721 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     6781 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     6841 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     6901 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     6961 TTT
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