LOCUS       RC208071                8028 bp    DNA     circular  SYN 
DEFINITION  HERC4 (Myc-DDK-tagged)-Human hect domain and RLD 4 (HERC4), transcript variant 2
ACCESSION   NM_015601
VERSION     NM_015601.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4185..4276 
                     /label="Myc-DDK"  
     misc_feature    4306..4891 
                     /label="PolyA signal"  
     misc_feature    4363..4382 
                     /label="Restriction Enzyme"  
     misc_feature    5167..5839 
                     /label="ColE1"  
     misc_feature    6269..7063 
                     /label="Kan/Neo"  
     misc_feature    7098..7400 
                     /label="SV40 ori"  
     misc_feature    7486..7963 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4167..4173 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4167 
                     /label="HERC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGTGCTGG GGAAATGCAT CCTTTGGGCA GCTAGGTTTG GGTGGAATTG 
     1081 ATGAAGAAAT TGTACTAGAG CCCAGAAAAA GTGACTTCTT TATAAATAAA AGGGTCCGAG 
     1141 ATGTAGGATG TGGACTCAGA CATACTGTGT TTGTTCTGGA TGATGGAACA GTGTACACAT 
     1201 GTGGATGTAA TGATCTAGGA CAGCTAGGTC ATGAAAAATC CAGAAAGAAA CCAGAGCAGG 
     1261 TTGTTGCCCT GGATGCCCAA AATATTGTAG CTGTTTCATG TGGAGAAGCT CATACGTTAG 
     1321 CGCTAAATGA CAAAGGCCAG GTGTATGCTT GGGGTCTCGA TTCTGATGGA CAGCTTGGCC 
     1381 TGGTAGGATC AGAGGAATGC ATCAGAGTAC CCAGAAATAT TAAAAGTTTG TCAGATATCC 
     1441 AGATTGTACA GGTTGCTTGT GGTTACTATC ATTCACTTGC ACTTTCTAAA GCAAGTGAAG 
     1501 TCTTCTGTTG GGGACAGAAT AAATATGGCC AATTGGGTTT AGGTACTGAC TGTAAAAAGC 
     1561 AAACTTCACC GCAGCTGCTT AAGTCTTTGC TTGGAATCCC TTTCATGCAA GTTGCAGCAG 
     1621 GAGGAGCCCA TAGTTTTGTA CTCACCCTTT CTGGAGCTAT CTTTGGATGG GGACGCAACA 
     1681 AGTTTGGTCA GCTAGGTCTT AATGATGAAA ATGATAGGTA TGTTCCTAAT TTACTAAAGT 
     1741 CACTAAGATC TCAGAAAATA GTTTATATTT GTTGTGGAGA AGATCATACT GCTGCTCTAA 
     1801 CCAAGGAAGG TGGAGTGTTT ACTTTTGGAG CTGGAGGGTA TGGTCAGTTG GGCCATAATT 
     1861 CTACCAGTCA TGAAATAAAC CCAAGGAAAG TTTTTGAACT TATGGGAAGC ATTGTCACTG 
     1921 AGATTGCTTG TGGACGGCAG CACACTTCTG CTTTTGTTCC TTCATCAGGA CGAATTTACT 
     1981 CTTTTGGGCT TGGTGGTAAT GGGCAGCTGG GAACCGGTTC AACAAGCAAC AGGAAAAGCC 
     2041 CCTTTACTGT AAAAGGAAAT TGGTACCCCT ATAATGGGCA GTGTCTACCA GATATTGATT 
     2101 CTGAAGAATA TTTCTGTGTA AAAAGAATTT TCTCAGGGGG AGATCAAAGC TTTTCACATT 
     2161 ACTCTAGTCC CCAGAACTGT GGGCCACCAG ATGACTTCAG ATGTCCCAAT CCGACAAAGC 
     2221 AGATCTGGAC AGTGAATGAA GCTCTAATTC AGAAATGGCT GAGCTATCCT TCTGGAAGGT 
     2281 TTCCTGTGGA GATAGCCAAT GAGATAGATG GAACGTTTTC TTCCTCTGGT TGCCTAAATG 
     2341 GAAGTTTTTT AGCTGTTAGC AATGATGATC ACTATAGAAC AGGTACCAGA TTTTCAGGGG 
     2401 TTGATATGAA TGCTGCTAGG CTTTTATTCC ACAAACTTAT ACAACCTGAT CATCCGCAGA 
     2461 TATCTCAGCA GGTGGCAGCT AGTTTGGAAA AGAATCTTAT TCCTAAACTG ACTAGCTCCT 
     2521 TACCTGATGT TGAAGCATTG AGGTTTTATC TTACTCTACC AGAATGTCCC CTGATGAGTG 
     2581 ATTCCAACAA TTTCACAACA ATAGCAATTC CCTTTGGTAC AGCTCTTGTG AACCTAGAAA 
     2641 AGGCACCACT GAAAGTACTT GAAAACTGGT GGTCAGTACT TGAACCTCCA CTATTCCTCA 
     2701 AGATAGTAGA ACTTTTTAAG GAAGTTGTGG TACATCTTTT GAAACTCTAC AAGATCGGTA 
     2761 TTCCCCCTTC TGAAAGAAGA ATTTTCAACA GTTTTCTTCA TACTGCATTA AAGGTTTTAG 
     2821 AAATACTACA TAGGGTAAAT GAGAAAATGG GACAGATTAT ACAGTATGAT AAATTTTATA 
     2881 TACATGAAGT ACAAGAATTG ATAGACATAA GAAATGATTA TATCAACTGG GTCCAACAGC 
     2941 AGGCCTATGG AATGTTGGCA GATATCCCTG TTACAATCTG TACATATCCA TTTGTATTTG 
     3001 ATGCCCAAGC AAAAACTACT CTGTTACAGA CCGATGCAGT CTTACAGATG CAGATGGCTA 
     3061 TTGATCAGGC CCACAGGCAG AATGTCTCCT CTCTTTTTCT CCCAGTGATT GAATCTGTGA 
     3121 ATCCCTGCTT AATTCTAGTG GTGCGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC 
     3181 TTAGGAAAAC AAAGAACATA GATTACAAGA AGCCACTCAA GGTTATATTT GTTGGAGAAG 
     3241 ATGCTGTGGA TGCAGGAGGG GTGCGCAAAG AATTTTTCTT GCTCATCATG AGGGAATTAT 
     3301 TGGATCCTAA ATACGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG 
     3361 ATAAGACATT TGAAGACAGT GATTTGTTCC ATTTGATTGG TGTTATCTGT GGCTTAGCAA 
     3421 TTTATAATTG TACCATTGTG GACCTCCATT TTCCTTTGGC TTTATATAAG AAACTACTGA 
     3481 AAAAGAAGCC ATCCTTGGAT GATTTGAAAG AACTAATGCC TGATGTTGGG AGAAGCATGC 
     3541 AACAGTTACT GGATTATCCA GAAGATGACA TAGAGGAAAC ATTTTGTCTT AATTTTACGA 
     3601 TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA 
     3661 CAGCTGTTAA CAAACAAAAT CGGCAAGAGT TTGTCGATGC TTATGTGGAT TACATATTCA 
     3721 ATAAATCAGT GGCTTCCTTA TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG 
     3781 GAAAAGTCCT TCTGCTCTTT CAGCCTAATG AACTACAAGC AATGGTCATT GGAAATACAA 
     3841 ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAATAT TGGGCAGAAC 
     3901 ATCCTACGAT AAAAATTTTT TGGGAAGTAT TTCACGAATT ACCATTGGAA AAGAAGAAAC 
     3961 AGTTTCTGTT ATTTTTGACA GGTAGTGATC GCATTCCTAT TCTTGGTATG AAGAGTCTGA 
     4021 AACTAGTCAT CCAGTCCACA GGAGGTGGTG AGGAGTATCT CCCAGTTTCC CATACTTGTT 
     4081 TTAATCTTCT GGATCTTCCA AAATATACAG AAAAAGAAAC TCTACGCTCT AAACTGATCC 
     4141 AAGCTATTGA TCACAATGAA GGCTTCAGTT TAATAACGCG TACGCGGCCG CTCGAGCAGA 
     4201 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4261 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4321 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4381 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4441 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4501 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4561 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4621 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4681 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4741 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4801 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4861 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4921 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4981 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5041 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5101 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5161 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5221 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5281 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5341 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5401 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5461 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5521 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5581 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5641 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5701 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5761 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5821 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5881 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5941 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6001 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6061 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6121 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6181 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6241 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6301 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6361 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6421 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6481 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6541 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6601 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6661 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6721 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6781 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6841 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6901 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6961 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7021 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7081 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7141 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7201 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7261 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7441 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7501 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7561 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7621 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7681 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7741 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7801 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7861 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7921 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7981 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//