LOCUS       RC208066                7185 bp    DNA     circular  SYN 
DEFINITION  CUL3 (Myc-DDK-tagged)-Human cullin 3 (CUL3)
ACCESSION   NM_003590
VERSION     NM_003590.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3342..3433 
                     /label="Myc-DDK"  
     misc_feature    3463..4048 
                     /label="PolyA signal"  
     misc_feature    3520..3539 
                     /label="Restriction Enzyme"  
     misc_feature    4324..4996 
                     /label="ColE1"  
     misc_feature    5426..6220 
                     /label="Kan/Neo"  
     misc_feature    6255..6557 
                     /label="SV40 ori"  
     misc_feature    6643..7120 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3324..3330 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3324 
                     /label="CUL3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAATCTG AGCAAAGGCA CGGGCAGCCG GAAGGACACC AAGATGCGGA 
     1081 TCCGGGCCTT TCCGATGACC ATGGATGAAA AATATGTAAA CAGCATTTGG GACCTTCTGA 
     1141 AAAATGCAAT TCAAGAAATC CAGCGTAAGA ATAACAGTGG TCTTAGTTTT GAGGAGCTCT 
     1201 ATAGAAATGC ATATACAATG GTTTTGCATA AACATGGAGA AAAGCTCTAC ACTGGACTAA 
     1261 GAGAAGTTGT TACCGAACAT CTCATAAATA AGGTGCGAGA AGATGTACTA AATTCATTGA 
     1321 ATAACAACTT TCTTCAAACG CTAAATCAAG CTTGGAATGA TCATCAAACA GCTATGGTGA 
     1381 TGATTAGAGA CATACTAATG TACATGGACC GTGTGTATGT ACAACAAAAT AATGTGGAGA 
     1441 ACGTCTACAA TTTGGGATTA ATTATTTTTC GAGATCAAGT TGTACGTTAT GGGTGTATTA 
     1501 GGGATCATCT ACGGCAAACT CTATTGGATA TGATTGCAAG AGAGCGGAAA GGAGAAGTCG 
     1561 TAGACAGAGG CGCAATAAGA AATGCTTGCC AGATGTTAAT GATTTTAGGT CTCGAAGGAA 
     1621 GATCAGTCTA TGAAGAAGAT TTTGAGGCTC CTTTTTTGGA AATGTCTGCA GAATTTTTTC 
     1681 AGATGGAAAG CCAGAAATTT TTAGCAGAAA ATAGTGCTTC AGTATATATA AAGAAAGTAG 
     1741 AAGCTAGAAT TAATGAAGAA ATAGAACGAG TGATGCACTG CCTTGACAAA TCAACGGAAG 
     1801 AACCAATTGT AAAGGTGGTT GAAAGGGAAC TCATTTCCAA GCACATGAAG ACTATAGTAG 
     1861 AAATGGAGAA TTCTGGGCTA GTACATATGT TGAAAAATGG AAAGACAGAA GACCTTGGTT 
     1921 GCATGTACAA GTTATTTAGT CGTGTGCCAA ATGGTTTGAA AACAATGTGT GAGTGTATGA 
     1981 GTTCCTATTT GAGGGAGCAA GGTAAAGCTC TTGTTTCTGA AGAAGGAGAA GGAAAGAATC 
     2041 CTGTTGACTA TATCCAGGGC TTATTGGATC TGAAGAGTAG GTTCGATCGC TTCCTCCTGG 
     2101 AATCATTCAA CAATGACCGT CTCTTTAAAC AAACTATTGC GGGTGACTTT GAGTATTTTC 
     2161 TCAACCTCAA CTCCAGGTCT CCTGAATACC TCTCATTATT TATTGATGAT AAGCTGAAAA 
     2221 AGGGAGTCAA AGGGCTAACA GAACAAGAAG TAGAAACAAT ATTGGATAAA GCAATGGTCC 
     2281 TTTTTAGGTT TATGCAAGAA AAAGATGTAT TTGAACGTTA TTATAAACAA CACTTGGCAA 
     2341 GGAGACTTCT CACAAATAAA AGTGTTTCTG ATGACTCTGA AAAAAACATG ATATCTAAGT 
     2401 TAAAGACTGA ATGTGGATGT CAGTTCACGT CAAAACTGGA AGGAATGTTT AGGGATATGA 
     2461 GCATCTCAAA CACAACGATG GATGAATTCA GGCAACATCT ACAGGCAACT GGTGTATCTT 
     2521 TAGGTGGTGT TGATCTTACA GTCCGGGTGC TCACGACAGG ATATTGGCCC ACTCAGTCAG 
     2581 CCACACCAAA GTGCAACATC CCACCAGCAC CAAGACATGC TTTTGAGATA TTCAGAAGGT 
     2641 TCTACTTAGC CAAACACAGT GGTCGACAGC TCACACTCCA GCATCATATG GGTTCTGCAG 
     2701 ATCTCAATGC CACATTTTAT GGACCAGTTA AAAAGGAAGA TGGATCTGAA GTTGGTGTTG 
     2761 GAGGTGCACA AGTAACTGGC TCTAATACAC GGAAGCACAT ATTGCAAGTT TCCACTTTCC 
     2821 AGATGACCAT ATTAATGCTC TTTAATAATA GAGAAAAATA CACATTTGAG GAAATTCAGC 
     2881 AAGAGACAGA TATCCCTGAA AGAGAGCTTG TTAGAGCCCT ACAGTCCCTC GCCTGTGGTA 
     2941 AACCAACACA GCGGGTTCTT ACAAAAGAAC CCAAATCAAA GGAAATAGAA AATGGTCATA 
     3001 TATTTACAGT TAATGATCAG TTCACATCCA AACTACACAG AGTCAAGATT CAAACAGTTG 
     3061 CTGCCAAACA AGGTGAATCC GACCCAGAGA GGAAAGAAAC AAGGCAGAAA GTAGACGACG 
     3121 ACAGAAAACA TGAGATAGAA GCTGCTATAG TGCGGATAAT GAAATCTAGA AAGAAGATGC 
     3181 AGCACAATGT TCTAGTAGCG GAGGTAACTC AGCAGTTGAA GGCGCGATTC TTACCAAGTC 
     3241 CAGTTGTTAT TAAGAAACGT ATTGAAGGAC TTATTGAGAG AGAATATTTG GCACGAACAC 
     3301 CTGAGGATCG CAAAGTATAC ACATATGTAG CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3361 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3421 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3481 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3541 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3601 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3661 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3721 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3781 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3841 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3901 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3961 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4021 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4081 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4141 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4201 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4261 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4321 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4381 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4441 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4501 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4561 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4621 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4681 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4741 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4801 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4861 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4921 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4981 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5041 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5101 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5161 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5221 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5281 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5341 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5401 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5461 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5521 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5581 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5641 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5701 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5761 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5821 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5881 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5941 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6001 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6061 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6121 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6181 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6241 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6301 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6361 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6421 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6481 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6541 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6601 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6661 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6721 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6781 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6841 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6901 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6961 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7021 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7081 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7141 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//