LOCUS       RC208051                7620 bp    DNA     circular  SYN 
DEFINITION  USP20 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 1
ACCESSION   NM_006676
VERSION     NM_006676.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3777..3868 
                     /label="Myc-DDK"  
     misc_feature    3898..4483 
                     /label="PolyA signal"  
     misc_feature    3955..3974 
                     /label="Restriction Enzyme"  
     misc_feature    4759..5431 
                     /label="ColE1"  
     misc_feature    5861..6655 
                     /label="Kan/Neo"  
     misc_feature    6690..6992 
                     /label="SV40 ori"  
     misc_feature    7078..7555 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3759..3765 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3759 
                     /label="USP20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGACTCC AGGGACCTTT GCCCTCACCT TGACTCCATA GGAGAGGTGA 
     1081 CCAAAGAGGA CTTGCTGCTC AAATCTAAGG GAACCTGTCA GTCGTGTGGG GTCACCGGAC 
     1141 CAAACCTATG GGCCTGTCTG CAGGTTGCCT GCCCCTATGT TGGCTGCGGA GAATCCTTCG 
     1201 CTGACCACAG CACCATTCAT GCACAGGCAA AAAAGCACAA CTTGACCGTG AACCTGACCA 
     1261 CGTTCCGACT GTGGTGTTAC GCCTGTGAGA AGGAGGTATT CCTGGAGCAG CGGCTGGCAG 
     1321 CCCCTCTGCT GGGCTCCTCT TCCAAGTTCT CTGAACAGGA CTCCCCGCCA CCCTCCCACC 
     1381 CTCTGAAAGC TGTTCCTATT GCTGTGGCTG ATGAAGGAGA GTCTGAGTCA GAGGATGATG 
     1441 ACCTGAAACC TCGAGGCCTC ACGGGCATGA AGAACCTCGG GAACTCCTGC TACATGAACG 
     1501 CCGCCCTGCA GGCCCTGTCC AATTGCCCGC CGCTGACTCA GTTCTTCTTG GAGTGTGGCG 
     1561 GCCTGGTGCG CACAGATAAG AAGCCAGCCC TGTGCAAGAG CTACCAGAAG CTGGTCTCTG 
     1621 AGGTCTGGCA TAAGAAACGG CCAAGCTACG TGGTCCCCAC CAGTCTGTCT CATGGGATCA 
     1681 AGTTGGTCAA CCCAATGTTC CGAGGCTATG CCCAGCAGGA CACCCAAGAG TTCCTTCGCT 
     1741 GCCTGATGGA CCAGCTGCAC GAGGAGCTCA AGGAGCCGGT GGTGGCCACG GTGGCGCTGA 
     1801 CGGAGGCTCG GGACTCAGAT TCGAGTGACA CGGATGAGAA ACGGGAGGGT GACCGGAGCC 
     1861 CATCAGAAGA TGAGTTCTTG TCCTGTGACT CGAGCAGTGA CCGGGGTGAG GGTGACGGGC 
     1921 AGGGGCGTGG CGGGGGCAGC TCGCAGGCCG AGACGGAGCT GCTGATCCCA GATGAGGCGG 
     1981 GCCGAGCCAT CTCTGAGAAG GAGCGGATGA AGGACCGCAA GTTCTCCTGG GGCCAGCAGC 
     2041 GTACAAACTC GGAGCAAGTG GACGAGGACG CTGATGTGGA CACTGCCATG GCTGCCCTTG 
     2101 ACCAGCCCGC GGAGGCCCAG CCCCCGTCAC CACGGTCCTC CAGCCCCTGC CGGACGCCAG 
     2161 AGCCGGACAA TGATGCTCAC CTACGCAGCT CCTCTCGCCC CTGCAGCCCC GTCCACCACC 
     2221 ACGAGGGCCA TGCCAAGCTG TCTAGCAGCC CCCCTCGTGC AAGCCCCGTG AGGATGGCAC 
     2281 CGTCGTACGT GCTCAAGAAA GCCCAGGTAT TGAGTGCTGG CAGCCGGAGG CGGAAGGAGC 
     2341 AGCGCTACCG CAGCGTCATC TCAGACATCT TTGACGGCTC CATTCTCAGC CTCGTGCAGT 
     2401 GTCTCACCTG TGACCGGGTA TCCACCACAG TGGAAACGTT CCAGGACTTA TCACTGCCCA 
     2461 TTCCTGGAAA GGAGGACCTG GCCAAGCTCC ATTCAGCCAT CTACCAGAAT GTGCCGGCCA 
     2521 AGCCAGGCGC CTGTGGGGAC AGCTATGCCG CCCAGGGCTG GCTGGCCTTC ATTGTGGAGT 
     2581 ACATCCGACG GTTTGTGGTA TCCTGTACCC CCAGCTGGTT TTGGGGGCCT GTCGTCACCC 
     2641 TGGAAGACTG CCTTGCTGCC TTCTTTGCCG CTGATGAGTT AAAGGGTGAC AACATGTACA 
     2701 GCTGTGAGCG GTGTAAGAAG CTGCGGAACG GAGTGAAGTA CTGCAAAGTC CTGCGGTTGC 
     2761 CCGAGATCCT GTGCATTCAC CTAAAGCGCT TTCGGCACGA GGTGATGTAC TCATTCAAGA 
     2821 TCAACAGCCA CGTCTCCTTC CCCCTCGAGG GGCTCGACCT GCGCCCCTTC CTTGCCAAGG 
     2881 AGTGCACATC CCAGATCACC ACCTACGACC TCCTCTCGGT CATCTGCCAC CACGGCACGG 
     2941 CAGGCAGTGG GCACTACATC GCCTACTGCC AGAACGTGAT CAATGGGCAG TGGTACGAGT 
     3001 TTGATGACCA GTACGTCACA GAAGTCCACG AGACGGTGGT GCAGAACGCC GAGGGCTACG 
     3061 TACTCTTCTA CAGGAAGAGC AGCGAGGAGG CCATGCGGGA GCGACAGCAG GTGGTGTCCC 
     3121 TGGCCGCCAT GCGGGAGCCC AGCCTGCTGC GGTTCTACGT GTCCCGCGAG TGGCTCAACA 
     3181 AGTTCAACAC CTTCGCGGAG CCAGGCCCCA TCACCAACCA GACCTTCCTC TGCTCCCACG 
     3241 GAGGCATCCC GCCCCACAAA TACCACTACA TCGACGACCT GGTGGTCATC CTGCCCCAGA 
     3301 ACGTCTGGGA GCACCTGTAC AACAGATTCG GGGGTGGCCC CGCCGTGAAC CACCTGTACG 
     3361 TGTGCTCCAT CTGCCAGGTG GAGATCGAGG CACTGGCCAA GCGCAGGAGG ATCGAGATCG 
     3421 ACACCTTCAT CAAGTTGAAC AAGGCCTTCC AGGCCGAGGA GTCGCCGGGC GTCATCTACT 
     3481 GCATCAGCAT GCAGTGGTTC CGGGAGTGGG AGGCGTTCGT CAAGGGGAAG GACAACGAGC 
     3541 CCCCCGGGCC CATTGACAAC AGCAGGATTG CACAGGTCAA AGGAAGCGGC CATGTCCAGC 
     3601 TGAAGCAGGG AGCTGACTAC GGGCAGATTT CGGAGGAGAC CTGGACCTAC CTGAACAGCC 
     3661 TGTATGGAGG TGGCCCCGAG ATTGCCATCC GCCAGAGTGT GGCGCAGCCG CTGGGCCCAG 
     3721 AGAACCTGCA CGGGGAGCAG AAGATCGAAG CCGAGACGCG GGCCGTGACG CGTACGCGGC 
     3781 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3841 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3901 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3961 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4021 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4081 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4141 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4201 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4261 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4321 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4381 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4441 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4501 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4561 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4621 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4681 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4741 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4861 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4921 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4981 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5041 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5101 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5161 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5221 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5281 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5341 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5401 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5461 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5521 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5581 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5641 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5701 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5761 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5821 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5881 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5941 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6001 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6061 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6121 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6181 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6241 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6301 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6361 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6421 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6481 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6541 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6601 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6661 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6721 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6781 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6841 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6961 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7021 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7081 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7141 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7201 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7261 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7321 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7381 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7441 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7501 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7561 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
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