LOCUS       RC208033L1                9231 bp    DNA     circular  SYN 
DEFINITION  GAA (Myc-DDK-tagged)-Human glucosidase, alpha, acid (GAA), transcript variant 3
ACCESSION   NM_001079804
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7522..7702 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8430..9089 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7861..8429 
                     /label="ColE1"  
     misc_feature    6397..6417 
                     /label="LR50 primer"  
     misc_feature    6194..6265 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6406..6997 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6176..6182 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6176 
                     /label="GAA"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGAGTGAGG CACCCGCCCT GCTCCCACCG 
     3361 GCTCCTGGCC GTCTGCGCCC TCGTGTCCTT GGCAACCGCT GCACTCCTGG GGCACATCCT 
     3421 ACTCCATGAT TTCCTGCTGG TTCCCCGAGA GCTGAGTGGC TCCTCCCCAG TCCTGGAGGA 
     3481 GACTCACCCA GCTCACCAGC AGGGAGCCAG TAGACCAGGG CCCCGGGATG CCCAGGCACA 
     3541 CCCCGGCCGT CCCAGAGCAG TGCCCACACA GTGCGACGTC CCCCCCAACA GCCGCTTCGA 
     3601 TTGCGCCCCT GACAAGGCCA TCACCCAGGA ACAGTGCGAG GCCCGCGGCT GTTGCTACAT 
     3661 CCCTGCAAAG CAGGGGCTGC AGGGAGCCCA GATGGGGCAG CCCTGGTGCT TCTTCCCACC 
     3721 CAGCTACCCC AGCTACAAGC TGGAGAACCT GAGCTCCTCT GAAATGGGCT ACACGGCCAC 
     3781 CCTGACCCGT ACCACCCCCA CCTTCTTCCC CAAGGACATC CTGACCCTGC GGCTGGACGT 
     3841 GATGATGGAG ACTGAGAACC GCCTCCACTT CACGATCAAA GATCCAGCTA ACAGGCGCTA 
     3901 CGAGGTGCCC TTGGAGACCC CGCATGTCCA CAGCCGGGCA CCGTCCCCAC TCTACAGCGT 
     3961 GGAGTTCTCC GAGGAGCCCT TCGGGGTGAT CGTGCGCCGG CAGCTGGACG GCCGCGTGCT 
     4021 GCTGAACACG ACGGTGGCGC CCCTGTTCTT TGCGGACCAG TTCCTTCAGC TGTCCACCTC 
     4081 GCTGCCCTCG CAGTATATCA CAGGCCTCGC CGAGCACCTC AGTCCCCTGA TGCTCAGCAC 
     4141 CAGCTGGACC AGGATCACCC TGTGGAACCG GGACCTTGCG CCCACGCCCG GTGCGAACCT 
     4201 CTACGGGTCT CACCCTTTCT ACCTGGCGCT GGAGGACGGC GGGTCGGCAC ACGGGGTGTT 
     4261 CCTGCTAAAC AGCAATGCCA TGGATGTGGT CCTGCAGCCG AGCCCTGCCC TTAGCTGGAG 
     4321 GTCGACAGGT GGGATCCTGG ATGTCTACAT CTTCCTGGGC CCAGAGCCCA AGAGCGTGGT 
     4381 GCAGCAGTAC CTGGACGTTG TGGGATACCC GTTCATGCCG CCATACTGGG GCCTGGGCTT 
     4441 CCACCTGTGC CGCTGGGGCT ACTCCTCCAC CGCTATCACC CGCCAGGTGG TGGAGAACAT 
     4501 GACCAGGGCC CACTTCCCCC TGGACGTCCA GTGGAACGAC CTGGACTACA TGGACTCCCG 
     4561 GAGGGACTTC ACGTTCAACA AGGATGGCTT CCGGGACTTC CCGGCCATGG TGCAGGAGCT 
     4621 GCACCAGGGC GGCCGGCGCT ACATGATGAT CGTGGATCCT GCCATCAGCA GCTCGGGCCC 
     4681 TGCCGGGAGC TACAGGCCCT ACGACGAGGG TCTGCGGAGG GGGGTTTTCA TCACCAACGA 
     4741 GACCGGCCAG CCGCTGATTG GGAAGGTATG GCCCGGGTCC ACTGCCTTCC CCGACTTCAC 
     4801 CAACCCCACA GCCCTGGCCT GGTGGGAGGA CATGGTGGCT GAGTTCCATG ACCAGGTGCC 
     4861 CTTCGACGGC ATGTGGATTG ACATGAACGA GCCTTCCAAC TTCATCAGGG GCTCTGAGGA 
     4921 CGGCTGCCCC AACAATGAGC TGGAGAACCC ACCCTACGTG CCTGGGGTGG TTGGGGGGAC 
     4981 CCTCCAGGCG GCCACCATCT GTGCCTCCAG CCACCAGTTT CTCTCCACAC ACTACAACCT 
     5041 GCACAACCTC TACGGCCTGA CCGAAGCCAT CGCCTCCCAC AGGGCGCTGG TGAAGGCTCG 
     5101 GGGGACACGC CCATTTGTGA TCTCCCGCTC GACCTTTGCT GGCCACGGCC GATACGCCGG 
     5161 CCACTGGACG GGGGACGTGT GGAGCTCCTG GGAGCAGCTC GCCTCCTCCG TGCCAGAAAT 
     5221 CCTGCAGTTT AACCTGCTGG GGGTGCCTCT GGTCGGGGCC GACGTCTGCG GCTTCCTGGG 
     5281 CAACACCTCA GAGGAGCTGT GTGTGCGCTG GACCCAGCTG GGGGCCTTCT ACCCCTTCAT 
     5341 GCGGAACCAC AACAGCCTGC TCAGTCTGCC CCAGGAGCCG TACAGCTTCA GCGAGCCGGC 
     5401 CCAGCAGGCC ATGAGGAAGG CCCTCACCCT GCGCTACGCA CTCCTCCCCC ACCTCTACAC 
     5461 ACTGTTCCAC CAGGCCCACG TCGCGGGGGA GACCGTGGCC CGGCCCCTCT TCCTGGAGTT 
     5521 CCCCAAGGAC TCTAGCACCT GGACTGTGGA CCACCAGCTC CTGTGGGGGG AGGCCCTGCT 
     5581 CATCACCCCA GTGCTCCAGG CCGGGAAGGC CGAAGTGACT GGCTACTTCC CCTTGGGCAC 
     5641 ATGGTACGAC CTGCAGACGG TGCCAGTAGA GGCCCTTGGC AGCCTCCCAC CCCCACCTGC 
     5701 AGCTCCCCGT GAGCCAGCCA TCCACAGCGA GGGGCAGTGG GTGACGCTGC CGGCCCCCCT 
     5761 GGACACCATC AACGTCCACC TCCGGGCTGG GTACATCATC CCCCTGCAGG GCCCTGGCCT 
     5821 CACAACCACA GAGTCCCGCC AGCAGCCCAT GGCCCTGGCT GTGGCCCTGA CCAAGGGTGG 
     5881 GGAGGCCCGA GGGGAGCTTT TCTGGGACGA TGGAGAGAGC CTGGAAGTGC TGGAGCGAGG 
     5941 GGCCTACACA CAGGTCATCT TCCTGGCCAG GAATAACACG ATCGTGAATG AGCTGGTACG 
     6001 TGTGACCAGT GAGGGAGCTG GCCTGCAGCT GCAGAAGGTG ACTGTCCTGG GCGTGGCCAC 
     6061 GGCGCCCCAG CAGGTCCTCT CCAACGGTGT CCCTGTCTCC AACTTCACCT ACAGCCCCGA 
     6121 CACCAAGGTC CTGGACATCT GTGTCTCGCT GTTGATGGGA GAGCAGTTTC TCGTCAGCTG 
     6181 GTGTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     6241 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA 
     6301 CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 
     6361 CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA 
     6421 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 
     6481 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 
     6541 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 
     6601 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 
     6661 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 
     6721 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 
     6781 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT 
     6841 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 
     6901 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 
     6961 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT 
     7021 CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC 
     7081 TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA 
     7141 GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA 
     7201 AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG 
     7261 GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT 
     7321 CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT 
     7381 AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT 
     7441 GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA 
     7501 CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 
     7561 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 
     7621 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 
     7681 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC 
     7741 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     7801 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     7861 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     7921 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     7981 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     8041 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     8101 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     8161 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     8221 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     8281 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     8341 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     8401 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA 
     8461 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA 
     8521 TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC 
     8581 ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG 
     8641 AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
     8701 TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 
     8761 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG 
     8821 GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG 
     8881 AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG 
     8941 TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
     9001 TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 
     9061 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT 
     9121 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     9181 AATAAACAAA TAGGGGTCCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C
//