LOCUS       RC208018                8235 bp    DNA     circular  SYN 
DEFINITION  UPF1 (Myc-DDK-tagged)-Human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1)
ACCESSION   NM_002911
VERSION     NM_002911.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4392..4483 
                     /label="Myc-DDK"  
     misc_feature    4513..5098 
                     /label="PolyA signal"  
     misc_feature    4570..4589 
                     /label="Restriction Enzyme"  
     misc_feature    5374..6046 
                     /label="ColE1"  
     misc_feature    6476..7270 
                     /label="Kan/Neo"  
     misc_feature    7305..7607 
                     /label="SV40 ori"  
     misc_feature    7693..8170 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4374..4380 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4374 
                     /label="UPF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGTGGAG GCGTACGGGC CCAGCTCGCA GACTCTCACT TTCCTGGACA 
     1081 CGGAGGAGGC CGAGCTGCTT GGCGCCGACA CACAGGGCTC CGAGTTCGAG TTCACCGACT 
     1141 TTACTCTTCC TAGCCAGACG CAGACGCCCC CCGGCGGCCC CGGCGGCCCG GGCGGTGGCG 
     1201 GCGCGGGAGG CCCGGGCGGC GCGGGCGCGG GCGCTGCGGC GGGACAGCTC GACGCGCAGG 
     1261 TTGGGCCCGA AGGCATCCTG CAGAACGGGG CTGTGGACGA CAGTGTAGCC AAGACCAGCC 
     1321 AGTTGTTGGC TGAGTTGAAC TTCGAGGAAG ATGAAGAAGA CACCTATTAC ACGAAGGACC 
     1381 TCCCCATACA CGCCTGCAGT TACTGTGGAA TACACGATCC TGCCTGCGTG GTTTACTGTA 
     1441 ATACCAGCAA GAAGTGGTTC TGCAACGGAC GTGGAAATAC TTCTGGCAGC CACATTGTAA 
     1501 ATCACCTTGT GAGGGCAAAA TGCAAAGAGG TGACCCTGCA CAAGGACGGG CCCCTGGGGG 
     1561 AGACAGTCCT GGAGTGCTAC AACTGCGGCT GTCGCAACGT CTTCCTCCTC GGCTTCATCC 
     1621 CGGCCAAAGC TGACTCAGTG GTGGTGCTGC TGTGCAGGCA GCCCTGTGCC AGCCAGAGCA 
     1681 GCCTCAAGGA CATCAACTGG GACAGCTCGC AGTGGCAGCC GCTGATCCAG GACCGCTGCT 
     1741 TCCTGTCCTG GCTGGTCAAG ATCCCCTCCG AGCAGGAGCA GCTGCGGGCA CGCCAGATCA 
     1801 CGGCACAGCA GATCAACAAG CTGGAGGAGC TGTGGAAGGA AAACCCTTCT GCCACGCTGG 
     1861 AGGACCTGGA GAAGCCGGGG GTGGACGAGG AGCCGCAGCA TGTCCTCCTG CGGTACGAGG 
     1921 ACGCCTACCA GTACCAGAAC ATATTCGGGC CCCTGGTCAA GCTGGAGGCC GACTACGACA 
     1981 AGAAGCTGAA GGAGTCCCAG ACTCAAGATA ACATCACTGT CAGGTGGGAC CTGGGCCTTA 
     2041 ACAAGAAGAG AATCGCCTAC TTCACTTTGC CCAAGACTGA CTCTGACATG CGGCTCATGC 
     2101 AGGGGGATGA GATATGCCTG CGGTACAAAG GGGACCTTGC GCCCCTGTGG AAAGGGATCG 
     2161 GCCACGTCAT CAAGGTCCCT GATAATTATG GCGATGAGAT CGCCATTGAG CTGCGGAGCA 
     2221 GCGTGGGTGC ACCTGTGGAG GTGACTCACA ACTTCCAGGT GGATTTTGTG TGGAAGTCGA 
     2281 CCTCCTTTGA CAGGATGCAG AGCGCATTGA AAACGTTTGC CGTGGATGAG ACCTCGGTGT 
     2341 CTGGCTACAT CTACCACAAG CTGTTGGGCC ACGAGGTGGA GGACGTAATC ATCAAGTGCC 
     2401 AGCTGCCCAA GCGCTTCACG GCGCAGGGCC TCCCCGACCT CAACCACTCC CAGGTTTATG 
     2461 CCGTGAAGAC TGTGCTGCAA AGACCACTGA GCCTGATCCA GGGCCCGCCA GGCACGGGGA 
     2521 AGACGGTGAC GTCGGCCACC ATCGTCTACC ACCTGGCCCG GCAAGGCAAC GGGCCGGTGC 
     2581 TGGTGTGTGC TCCGAGCAAC ATCGCCGTGG ACCAGCTAAC GGAGAAGATC CACCAGACGG 
     2641 GGCTAAAGGT CGTGCGCCTC TGCGCCAAGA GCCGTGAGGC CATCGACTCC CCGGTGTCTT 
     2701 TTCTGGCCCT GCACAACCAG ATCAGGAACA TGGACAGCAT GCCTGAGCTG CAGAAGCTGC 
     2761 AGCAGCTGAA AGACGAGACT GGGGAGCTGT CGTCTGCCGA CGAGAAGCGG TACCGGGCCT 
     2821 TGAAGCGCAC CGCAGAGAGA GAGCTGCTGA TGAACGCAGA TGTCATCTGC TGCACATGTG 
     2881 TGGGCGCCGG TGACCCGAGG CTGGCCAAGA TGCAGTTCCG CTCCATTTTA ATCGACGAAA 
     2941 GCACCCAGGC CACCGAGCCG GAGTGCATGG TTCCCGTGGT CCTCGGGGCC AAGCAGCTGA 
     3001 TCCTTGTAGG CGACCACTGC CAGCTGGGCC CAGTGGTGAT GTGCAAGAAG GCGGCCAAGG 
     3061 CCGGGCTGTC ACAGTCGCTC TTCGAGCGCC TGGTGGTGCT GGGCATCCGG CCCATCCGCC 
     3121 TGCAGGTCCA GTACCGGATG CACCCTGCAC TCAGCGCCTT CCCATCCAAC ATCTTCTACG 
     3181 AGGGCTCCCT CCAGAATGGT GTCACTGCAG CGGATCGTGT GAAGAAGGGA TTTGACTTCC 
     3241 AGTGGCCCCA ACCCGATAAA CCGATGTTCT TCTACGTGAC CCAGGGCCAA GAGGAGATTG 
     3301 CCAGCTCGGG CACCTCCTAC CTGAACAGGA CCGAGGCTGC GAACGTGGAG AAGATCACCA 
     3361 CGAAGTTGCT GAAGGCAGGC GCCAAGCCGG ACCAGATTGG CATCATCACG CCCTACGAGG 
     3421 GCCAGCGCTC CTACCTGGTG CAGTACATGC AGTTCAGCGG CTCCCTGCAC ACCAAGCTCT 
     3481 ACCAGGAGGT GGAGATCGCC AGTGTGGACG CCTTTCAGGG ACGCGAGAAG GACTTCATCA 
     3541 TCCTGTCCTG TGTGCGGGCC AACGAGCACC AAGGCATTGG CTTTTTAAAT GACCCCAGGC 
     3601 GTCTGAACGT GGCCCTGACC AGAGCAAGGT ATGGCGTCAT CATTGTGGGC AACCCGAAGG 
     3661 CACTATCAAA GCAGCCGCTC TGGAACCACC TGCTGAACTA CTATAAGGAG CAGAAGGTGC 
     3721 TGGTGGAGGG GCCGCTCAAC AACCTGCGTG AGAGCCTCAT GCAGTTCAGC AAGCCACGGA 
     3781 AGCTGGTCAA CACTATCAAC CCGGGAGCCC GCTTCATGAC CACAGCCATG TATGATGCCC 
     3841 GGGAGGCCAT CATCCCAGGC TCCGTCTATG ATCGGAGCAG CCAGGGCCGG CCTTCCAGCA 
     3901 TGTACTTCCA GACCCATGAC CAGATTGGCA TGATCAGTGC CGGCCCTAGC CACGTGGCTG 
     3961 CCATGAACAT TCCCATCCCC TTCAACCTGG TCATGCCACC CATGCCACCG CCTGGCTATT 
     4021 TTGGACAAGC CAACGGGCCT GCTGCAGGGC GAGGCACCCC GAAAGGCAAG ACTGGTCGTG 
     4081 GGGGACGCCA GAAGAACCGC TTTGGGCTTC CTGGACCCAG CCAGACTAAC CTCCCCAACA 
     4141 GCCAAGCCAG CCAGGATGTG GCGTCACAGC CCTTCTCTCA GGGCGCCCTG ACGCAGGGCT 
     4201 ACATCTCCAT GAGCCAGCCT TCCCAGATGA GCCAGCCCGG CCTCTCCCAG CCGGAGCTGT 
     4261 CCCAGGACAG TTACCTTGGT GACGAGTTTA AATCACAAAT CGACGTGGCG CTCTCACAGG 
     4321 ACTCCACGTA CCAGGGAGAG CGGGCTTACC AGCATGGCGG GGTGACGGGG CTGTCCCAGT 
     4381 ATACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4441 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4501 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4561 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4621 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4681 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4741 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4801 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4861 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4921 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4981 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5041 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5101 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5161 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5221 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5281 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5341 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5401 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5461 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5521 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5581 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5641 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5701 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5761 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5821 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5881 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5941 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6001 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6061 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6121 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6181 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6241 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6301 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6361 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6421 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6481 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6541 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6601 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6661 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6721 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6781 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6841 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6901 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6961 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7021 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7081 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7141 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7201 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7261 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7321 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7381 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7441 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7501 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7681 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7741 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7801 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7861 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7921 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7981 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8041 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8101 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8161 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8221 TTTAACGCGA ATTTT
//