LOCUS       RC207991L1                9357 bp    DNA     circular  SYN 
DEFINITION  NLRP4 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 4 (NLRP4)
ACCESSION   NM_134444
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7648..7828 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8556..9215 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7987..8555 
                     /label="ColE1"  
     misc_feature    6523..6543 
                     /label="LR50 primer"  
     misc_feature    6320..6391 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6532..7123 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6302..6308 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6302 
                     /label="NLRP4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCAGCCTCT TTCTTCTCTG ATTTTGGTCT 
     3361 TATGTGGTAT CTGGAGGAGC TCAAAAAGGA GGAGTTCAGG AAATTTAAAG AACATCTCAA 
     3421 GCAAATGACT TTGCAGCTTG AACTCAAGCA GATTCCCTGG ACTGAGGTCA AAAAAGCATC 
     3481 CCGGGAAGAA CTTGCAAACC TCTTGATCAA GCACTATGAA GAACAACAAG CTTGGAACAT 
     3541 AACCTTAAGA ATCTTTCAAA AGATGGATAG AAAGGATCTC TGCATGAAGG TCATGAGGGA 
     3601 GAGAACAGGA TACACAAAGA CCTATCAAGC TCACGCAAAG CAGAAATTCA GCCGCTTATG 
     3661 GTCCAGCAAG TCTGTCACTG AGATTCACCT ATACTTTGAG GAGGAAGTCA AGCAAGAAGA 
     3721 ATGTGACCAT TTGGACCGCC TTTTTGCTCC CAAGGAAGCT GGGAAACAGC CACGTACAGT 
     3781 GATCATTCAA GGACCACAAG GAATTGGAAA AACGACACTC CTGATGAAGC TGATGATGGC 
     3841 CTGGTCGGAC AACAAGATCT TTCGGGATAG GTTCCTGTAC ACATTCTATT TCTGCTGCAG 
     3901 AGAACTGAGG GAGTTGCCGC CAACGAGTTT GGCTGACTTG ATTTCCAGAG AGTGGCCTGA 
     3961 CCCCGCTGCT CCTATAACAG AGATCGTGTC TCAACCGGAG AGACTCTTGT TCGTCATCGA 
     4021 CAGCTTCGAA GAGCTGCAGG GCGGCTTGAA CGAACCCGAT TCGGATCTGT GTGGTGACTT 
     4081 GATGGAGAAA CGGCCGGTGC AGGTGCTTCT GAGCAGTTTG CTGAGGAAGA AGATGCTCCC 
     4141 GGAGGCCTCC CTGCTCATCG CTATCAAACC CGTGTGCCCG AAGGAGCTCC GGGATCAGGT 
     4201 GACGATCTCA GAAATCTACC AGCCCCGGGG ATTCAACGAG AGTGATAGGT TAGTGTATTT 
     4261 CTGCTGTTTC TTCAAAGACC CGAAAAGAGC CATGGAAGCC TTCAATCTTG TAAGAGAAAG 
     4321 TGAACAGCTG TTTTCCATAT GCCAAATCCC GCTCCTCTGC TGGATCCTGT GTACCAGTCT 
     4381 GAAGCAAGAG ATGCAGAAAG GAAAAGACCT GGCCCTGACC TGCCAGAGCA CTACCTCTGT 
     4441 GTACTCCTCT TTCGTCTTTA ACCTGTTCAC ACCTGACGGT GCCGAGGGCC CGACTCAGCA 
     4501 AACCCAGCAC CAGCTGAAGG CCCTGTGCTC CCTGGCTGCA GAGGGTATGT GGACAGACAC 
     4561 ATTTGAGTTT TGTGAAGACG ACCTCCGGAG AAATGGGGTT GTTGACGCTG ACATCCCTGC 
     4621 GCTGCTGGGC ACCAAGATAC TTCTGAAGTA CGGGGAGCGT GAGAGCTCCT ACGTGTTCCT 
     4681 CCACGTGTGT ATCCAGGAGT TCTGTGCCGC CTTGTTCTAT TTGCTCAAGA GCCACCTTGA 
     4741 TCATCCTCAC CCAGCTGTGA GATGTGTACA GGAATTGCTA GTTGCCAATT TTGAAAAAGC 
     4801 AAGGAGAGCA CATTGGATTT TTTTGGGGTG TTTTCTAACT GGCCTTTTAA ATAAAAAGGA 
     4861 ACAAGAAAAA CTGGATGCGT TTTTTGGCTT CCAACTGTCC CAAGAGATAA AGCAGCAAAT 
     4921 TCACCAGTGC CTGAAGAGCT TAGGGGAGCG TGGCAATCCT CAGGGACAGG TGGATTCCTT 
     4981 GGCGATATTT TACTGTCTCT TTGAAATGCA GGATCCTGCC TTTGTGAAGC AGGCAGTGAA 
     5041 CCTCCTCCAA GAAGCTAACT TTCATATTAT TGACAACGTG GACTTGGTGG TTTCTGCCTA 
     5101 CTGCTTAAAA TACTGCTCCA GCTTGAGGAA ACTCTGTTTT TCCGTTCAAA ATGTCTTTAA 
     5161 GAAAGAGGAT GAACACAGCT CTACGTCGGA TTACAGCCTC ATCTGTTGGC ATCACATCTG 
     5221 CTCTGTGCTC ACCACCAGCG GGCACCTCAG AGAGCTCCAG GTGCAGGACA GCACCCTCAG 
     5281 CGAGTCGACC TTTGTGACCT GGTGTAACCA GCTGAGGCAT CCCAGCTGTC GCCTTCAGAA 
     5341 GCTTGGAATA AATAACGTTT CCTTTTCTGG CCAGAGTGTT CTGCTCTTTG AGGTGCTCTT 
     5401 TTATCAGCCA GACTTGAAAT ACCTGAGCTT CACCCTCACG AAACTCTCTC GTGATGACAT 
     5461 CAGGTCCCTC TGTGATGCCT TGAACTACCC AGCAGGCAAC GTCAAAGAGC TAGCGCTGGT 
     5521 AAATTGTCAC CTCTCACCCA TTGATTGTGA AGTCCTTGCT GGCCTTCTAA CCAACAACAA 
     5581 GAAGCTGACG TATCTGAATG TATCCTGCAA CCAGTTAGAC ACAGGCGTGC CCCTTTTGTG 
     5641 TGAAGCCATG TGCAGCCCAG ACACGGTCCT GGTATACCTG ATGTTGGCTT TCTGCCACCT 
     5701 CAGCGAGCAG TGCTGCGAAT ACATCTCTGA AATGCTTCTG CGTAACAAGA GCGTGCGCTA 
     5761 TCTAGACCTC AGTGCCAATG TCCTGAAGGA CGAAGGACTG AAAACTCTCT GCGAGGCCTT 
     5821 GAAACATCCG GACTGCTGCC TGGATTCACT GTGTTTGGTA AAATGTTTTA TCACTGCTGC 
     5881 TGGCTGTGAA GACCTCGCCT CTGCTCTCAT CAGCAATCAA AACCTGAAGA TTCTGCAAAT 
     5941 TGGGTGCAAT GAAATCGGAG ATGTGGGTGT GCAGCTGTTG TGTCGGGCTC TGACGCATAC 
     6001 GGATTGCCGC TTAGAGATTC TTGGGTTGGA AGAATGTGGG TTAACGAGCA CCTGCTGTAA 
     6061 GGATCTCGCG TCTGTTCTCA CCTGCAGTAA GACCCTGCAG CTGCTCAACC TGACCTTGAA 
     6121 CACCTTGGAC CACACAGGGG TGGTTGTACT CTGTGAGGCC CTGAGACACC CAGAGTGTGC 
     6181 CCTGCAGGTG CTCGGGCTGA GAAAAACTGA TTTTGATGAG GAAACCCAGG CACTGCTGAC 
     6241 GGCTGAGGAA GAGAGAAATC CTAACCTGAC CATCACAGAC GACTGTGACA CAATCACAAG 
     6301 GGTAGAGATC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC 
     6361 AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG 
     6421 TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA 
     6481 GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT TCGATATCAA GCTTATCGAT 
     6541 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 
     6601 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 
     6661 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 
     6721 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 
     6781 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 
     6841 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 
     6901 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 
     6961 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 
     7021 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 
     7081 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 
     7141 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC 
     7201 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC 
     7261 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 
     7321 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA 
     7381 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC 
     7441 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA 
     7501 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA 
     7561 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT 
     7621 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT 
     7681 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 
     7741 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 
     7801 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA 
     7861 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     7921 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     7981 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     8041 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     8101 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     8161 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     8221 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     8281 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     8341 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     8401 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     8461 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     8521 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGATTACGC CCCGCCCTGC 
     8581 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 
     8641 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 
     8701 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA 
     8761 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA 
     8821 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC 
     8881 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG 
     8941 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC 
     9001 ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA 
     9061 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC 
     9121 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT 
     9181 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATACTCTT CCTTTTTCAA 
     9241 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     9301 TAGAAAAATA AACAAATAGG GGTCCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAC
//