LOCUS       RC207991                7863 bp    DNA     circular  SYN 
DEFINITION  NLRP4 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 4 (NLRP4)
ACCESSION   NM_134444
VERSION     NM_134444.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4020..4111 
                     /label="Myc-DDK"  
     misc_feature    4141..4726 
                     /label="PolyA signal"  
     misc_feature    4198..4217 
                     /label="Restriction Enzyme"  
     misc_feature    5002..5674 
                     /label="ColE1"  
     misc_feature    6104..6898 
                     /label="Kan/Neo"  
     misc_feature    6933..7235 
                     /label="SV40 ori"  
     misc_feature    7321..7798 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4002..4008 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4002 
                     /label="NLRP4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCTCT TTCTTCTCTG ATTTTGGTCT TATGTGGTAT CTGGAGGAGC 
     1081 TCAAAAAGGA GGAGTTCAGG AAATTTAAAG AACATCTCAA GCAAATGACT TTGCAGCTTG 
     1141 AACTCAAGCA GATTCCCTGG ACTGAGGTCA AAAAAGCATC CCGGGAAGAA CTTGCAAACC 
     1201 TCTTGATCAA GCACTATGAA GAACAACAAG CTTGGAACAT AACCTTAAGA ATCTTTCAAA 
     1261 AGATGGATAG AAAGGATCTC TGCATGAAGG TCATGAGGGA GAGAACAGGA TACACAAAGA 
     1321 CCTATCAAGC TCACGCAAAG CAGAAATTCA GCCGCTTATG GTCCAGCAAG TCTGTCACTG 
     1381 AGATTCACCT ATACTTTGAG GAGGAAGTCA AGCAAGAAGA ATGTGACCAT TTGGACCGCC 
     1441 TTTTTGCTCC CAAGGAAGCT GGGAAACAGC CACGTACAGT GATCATTCAA GGACCACAAG 
     1501 GAATTGGAAA AACGACACTC CTGATGAAGC TGATGATGGC CTGGTCGGAC AACAAGATCT 
     1561 TTCGGGATAG GTTCCTGTAC ACATTCTATT TCTGCTGCAG AGAACTGAGG GAGTTGCCGC 
     1621 CAACGAGTTT GGCTGACTTG ATTTCCAGAG AGTGGCCTGA CCCCGCTGCT CCTATAACAG 
     1681 AGATCGTGTC TCAACCGGAG AGACTCTTGT TCGTCATCGA CAGCTTCGAA GAGCTGCAGG 
     1741 GCGGCTTGAA CGAACCCGAT TCGGATCTGT GTGGTGACTT GATGGAGAAA CGGCCGGTGC 
     1801 AGGTGCTTCT GAGCAGTTTG CTGAGGAAGA AGATGCTCCC GGAGGCCTCC CTGCTCATCG 
     1861 CTATCAAACC CGTGTGCCCG AAGGAGCTCC GGGATCAGGT GACGATCTCA GAAATCTACC 
     1921 AGCCCCGGGG ATTCAACGAG AGTGATAGGT TAGTGTATTT CTGCTGTTTC TTCAAAGACC 
     1981 CGAAAAGAGC CATGGAAGCC TTCAATCTTG TAAGAGAAAG TGAACAGCTG TTTTCCATAT 
     2041 GCCAAATCCC GCTCCTCTGC TGGATCCTGT GTACCAGTCT GAAGCAAGAG ATGCAGAAAG 
     2101 GAAAAGACCT GGCCCTGACC TGCCAGAGCA CTACCTCTGT GTACTCCTCT TTCGTCTTTA 
     2161 ACCTGTTCAC ACCTGACGGT GCCGAGGGCC CGACTCAGCA AACCCAGCAC CAGCTGAAGG 
     2221 CCCTGTGCTC CCTGGCTGCA GAGGGTATGT GGACAGACAC ATTTGAGTTT TGTGAAGACG 
     2281 ACCTCCGGAG AAATGGGGTT GTTGACGCTG ACATCCCTGC GCTGCTGGGC ACCAAGATAC 
     2341 TTCTGAAGTA CGGGGAGCGT GAGAGCTCCT ACGTGTTCCT CCACGTGTGT ATCCAGGAGT 
     2401 TCTGTGCCGC CTTGTTCTAT TTGCTCAAGA GCCACCTTGA TCATCCTCAC CCAGCTGTGA 
     2461 GATGTGTACA GGAATTGCTA GTTGCCAATT TTGAAAAAGC AAGGAGAGCA CATTGGATTT 
     2521 TTTTGGGGTG TTTTCTAACT GGCCTTTTAA ATAAAAAGGA ACAAGAAAAA CTGGATGCGT 
     2581 TTTTTGGCTT CCAACTGTCC CAAGAGATAA AGCAGCAAAT TCACCAGTGC CTGAAGAGCT 
     2641 TAGGGGAGCG TGGCAATCCT CAGGGACAGG TGGATTCCTT GGCGATATTT TACTGTCTCT 
     2701 TTGAAATGCA GGATCCTGCC TTTGTGAAGC AGGCAGTGAA CCTCCTCCAA GAAGCTAACT 
     2761 TTCATATTAT TGACAACGTG GACTTGGTGG TTTCTGCCTA CTGCTTAAAA TACTGCTCCA 
     2821 GCTTGAGGAA ACTCTGTTTT TCCGTTCAAA ATGTCTTTAA GAAAGAGGAT GAACACAGCT 
     2881 CTACGTCGGA TTACAGCCTC ATCTGTTGGC ATCACATCTG CTCTGTGCTC ACCACCAGCG 
     2941 GGCACCTCAG AGAGCTCCAG GTGCAGGACA GCACCCTCAG CGAGTCGACC TTTGTGACCT 
     3001 GGTGTAACCA GCTGAGGCAT CCCAGCTGTC GCCTTCAGAA GCTTGGAATA AATAACGTTT 
     3061 CCTTTTCTGG CCAGAGTGTT CTGCTCTTTG AGGTGCTCTT TTATCAGCCA GACTTGAAAT 
     3121 ACCTGAGCTT CACCCTCACG AAACTCTCTC GTGATGACAT CAGGTCCCTC TGTGATGCCT 
     3181 TGAACTACCC AGCAGGCAAC GTCAAAGAGC TAGCGCTGGT AAATTGTCAC CTCTCACCCA 
     3241 TTGATTGTGA AGTCCTTGCT GGCCTTCTAA CCAACAACAA GAAGCTGACG TATCTGAATG 
     3301 TATCCTGCAA CCAGTTAGAC ACAGGCGTGC CCCTTTTGTG TGAAGCCATG TGCAGCCCAG 
     3361 ACACGGTCCT GGTATACCTG ATGTTGGCTT TCTGCCACCT CAGCGAGCAG TGCTGCGAAT 
     3421 ACATCTCTGA AATGCTTCTG CGTAACAAGA GCGTGCGCTA TCTAGACCTC AGTGCCAATG 
     3481 TCCTGAAGGA CGAAGGACTG AAAACTCTCT GCGAGGCCTT GAAACATCCG GACTGCTGCC 
     3541 TGGATTCACT GTGTTTGGTA AAATGTTTTA TCACTGCTGC TGGCTGTGAA GACCTCGCCT 
     3601 CTGCTCTCAT CAGCAATCAA AACCTGAAGA TTCTGCAAAT TGGGTGCAAT GAAATCGGAG 
     3661 ATGTGGGTGT GCAGCTGTTG TGTCGGGCTC TGACGCATAC GGATTGCCGC TTAGAGATTC 
     3721 TTGGGTTGGA AGAATGTGGG TTAACGAGCA CCTGCTGTAA GGATCTCGCG TCTGTTCTCA 
     3781 CCTGCAGTAA GACCCTGCAG CTGCTCAACC TGACCTTGAA CACCTTGGAC CACACAGGGG 
     3841 TGGTTGTACT CTGTGAGGCC CTGAGACACC CAGAGTGTGC CCTGCAGGTG CTCGGGCTGA 
     3901 GAAAAACTGA TTTTGATGAG GAAACCCAGG CACTGCTGAC GGCTGAGGAA GAGAGAAATC 
     3961 CTAACCTGAC CATCACAGAC GACTGTGACA CAATCACAAG GGTAGAGATC ACGCGTACGC 
     4021 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4081 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4141 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4201 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4261 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4321 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4381 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4441 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4501 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4561 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4621 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4681 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4741 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4801 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4861 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4921 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4981 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5041 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5101 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5161 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5221 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5281 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5341 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5401 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5461 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5521 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5581 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5641 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5701 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5761 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5821 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5881 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5941 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6001 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6061 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6121 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6181 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6241 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6301 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6361 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6421 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6481 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6541 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6601 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6661 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6721 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6781 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6841 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6901 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6961 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7021 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7081 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7141 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7261 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7321 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7381 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7441 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7501 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7561 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7621 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7681 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7741 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7801 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7861 TTT
//