LOCUS       RC207980                8385 bp    DNA     circular  SYN 
DEFINITION  TBC1D1 (Myc-DDK-tagged)-Human TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (TBC1D1)
ACCESSION   NM_015173
VERSION     NM_015173.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4542..4633 
                     /label="Myc-DDK"  
     misc_feature    4663..5248 
                     /label="PolyA signal"  
     misc_feature    4720..4739 
                     /label="Restriction Enzyme"  
     misc_feature    5524..6196 
                     /label="ColE1"  
     misc_feature    6626..7420 
                     /label="Kan/Neo"  
     misc_feature    7455..7757 
                     /label="SV40 ori"  
     misc_feature    7843..8320 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4524..4530 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4524 
                     /label="TBC1D1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCAATA ACATTCACAG CAAGGAAACA TCTGCTTTCT AACGAGGTCT 
     1081 CGGTGGATTT TGGCCTGCAG CTGGTGGGCT CCCTGCCTGT GCATTCCCTG ACCACCATGC 
     1141 CCATGCTGCC CTGGGTTGTG GCTGAGGTGC GAAGACTCAG CAGGCAGTCC ACCAGAAAGG 
     1201 AACCTGTAAC CAAGCAAGTC CGGCTTTGCG TTTCACCCTC TGGACTGAGA TGTGAACCTG 
     1261 AGCCAGGGAG AAGTCAACAG TGGGATCCCC TGATCTATTC CAGCATCTTT GAGTGCAAGC 
     1321 CTCAGCGTGT TCACAAACTG ATTCACAACA GTCATGACCC AAGTTACTTT GCTTGTCTGA 
     1381 TTAAGGAAGA CGCTGTCCAC CGGCAGAGTA TCTGCTATGT GTTCAAAGCC GATGATCAAA 
     1441 CAAAAGTGCC TGAGATCATC AGCTCCATCC GTCAGGCGGG GAAGATCGCC CGGCAGGAGG 
     1501 AGCTGCACTG CCCGTCCGAG TTCGACGACA CGTTTTCCAA GAAGTTCGAG GTGCTCTTCT 
     1561 GCGGCCGCGT GACGGTGGCG CACAAGAAGG CTCCGCCGGC CCTGATCGAC GAGTGCATCG 
     1621 AGAAGTTCAA TCACGTCAGC GGCAGCCGGG GGTCCGAGAG CCCCCGCCCC AACCCGCCCC 
     1681 ATGCCGCGCC CACAGGGAGC CAGGAGCCTG TGCGCAGGCC CATGCGCAAG TCCTTCTCCC 
     1741 AGCCCGGCCT GCGCTCGCTG GCCTTTAGGA AGGAGCTGCA GGATGGGGGC CTCCGAAGCA 
     1801 GCGGCTTCTT CAGCTCCTTC GAGGAGAGCG ACATTGAGAA CCACCTCATT AGCGGACACA 
     1861 ATATTGTGCA GCCCACAGAT ATCGAGGAAA ATCGAACTAT GCTCTTCACG ATTGGCCAGT 
     1921 CTGAAGTTTA CCTCATCAGT CCTGACACCA AAAAAATAGC ATTGGAGAAA AATTTTAAGG 
     1981 AGATATCCTT TTGCTCTCAG GGCATCAGAC ACGTGGACCA CTTTGGGTTT ATCTGTCGGG 
     2041 AGTCTTCCGG AGGTGGCGGC TTTCATTTTG TCTGTTACGT GTTTCAGTGC ACAAATGAGG 
     2101 CTCTGGTTGA TGAAATTATG ATGACCCTGA AACAGGCCTT CACGGTGGCC GCAGTGCAGC 
     2161 AGACAGCTAA GGCGCCAGCC CAGCTGTGTG AGGGCTGCCC CCTGCAAAGC CTGCACAAGC 
     2221 TCTGTGAGAG GATAGAGGGA ATGAATTCTT CCAAAACAAA ACTAGAACTG CAAAAGCACC 
     2281 TGACGACATT AACAAATCAG GAGCAGGCGA CTATTTTTGA AGAGGTTCAG AAATTGAGAC 
     2341 CGAGAAATGA GCAGCGAGAG AATGAATTGA TTATTTCTTT TCTGAGATGT TTATATGAAG 
     2401 AGAAACAGAA AGAACACATC CATATTGGGG AGATGAAGCA GACATCGCAG ATGGCAGCAG 
     2461 AGAATATTGG AAGTGAATTA CCACCCAGTG CCACTCGATT TAGGCTAGAT ATGCTGAAAA 
     2521 ACAAAGCAAA GAGATCTTTA ACAGAGTCTT TAGAAAGTAT TTTGTCCCGG GGTAATAAAG 
     2581 CCAGAGGCCT GCAGGAACAC TCCATCAGTG TGGATCTGGA TAGCTCCCTG TCTAGTACAT 
     2641 TAAGTAACAC CAGCAAAGAG CCATCTGTGT GTGAAAAGGA GGCCTTGCCC ATCTCTGAGA 
     2701 GCTCCTTTAA GCTCCTCGGC TCCTCGGAGG ACCTGTCCAG TGACTCGGAG AGTCATCTCC 
     2761 CAGAAGAGCC AGCTCCGCTG TCGCCCCAGC AGGCCTTCAG GAGGCGAGCA AACACCCTGA 
     2821 GTCACTTCCC CATCGAATGC CAGGAACCTC CACAACCTGC CCGGGGGTCC CCGGGGGTTT 
     2881 CGCAAAGGAA ACTTATGAGG TATCACTCAG TGAGCACAGA GACGCCTCAT GAACGAAAGG 
     2941 ACTTTGAATC CAAAGCAAAC CATCTTGGTG ATTCTGGTGG GACTCCTGTG AAGACCCGGA 
     3001 GGCATTCCTG GAGGCAGCAG ATATTCCTCC GAGTAGCCAC CCCGCAGAAG GCGTGCGATT 
     3061 CTTCCAGCAG ATATGAAGAT TATTCAGAGC TGGGAGAGCT TCCCCCACGA TCTCCTTTAG 
     3121 AACCAGTTTG TGAAGATGGG CCCTTTGGCC CCCCACCAGA GGAAAAGAAA AGGACATCTC 
     3181 GTGAGCTCCG AGAGCTGTGG CAAAAGGCTA TTCTTAAACA GATACTGCTG CTTAGAATGG 
     3241 AGAAGGAAAA TCAGAAGCTC CAAGCCTCTG AAAATGATTT GCTGAACAAG CGCCTGAAGC 
     3301 TCGATTATGA AGAAATTACT CCCTGTCTTA AAGAAGTAAC TACAGTGTGG GAAAAGATGC 
     3361 TTAGCACTCC AGGAAGATCA AAAATTAAGT TTGACATGGA AAAAATGCAC TCGGCTGTTG 
     3421 GGCAAGGTGT GCCACGTCAT CACCGAGGTG AAATCTGGAA ATTTCTAGCT GAGCAATTCC 
     3481 ACCTTAAACA CCAGTTTCCC AGCAAACAGC AGCCAAAGGA TGTGCCATAC AAAGAACTCT 
     3541 TAAAGCAGCT GACTTCCCAG CAGCATGCGA TTCTTATTGA CCTTGGGCGA ACCTTTCCTA 
     3601 CACACCCATA CTTCTCTGCC CAGCTTGGAG CAGGACAGCT ATCGCTTTAC AACATTTTGA 
     3661 AGGCCTACTC ACTTCTAGAC CAGGAAGTGG GATATTGCCA AGGTCTCAGC TTTGTAGCAG 
     3721 GCATTTTGCT TCTTCATATG AGTGAGGAAG AGGCGTTTAA AATGCTCAAG TTTCTGATGT 
     3781 TTGACATGGG GCTGCGGAAA CAGTATCGGC CAGACATGAT TATTTTACAG ATCCAGATGT 
     3841 ACCAGCTCTC GAGGTTGCTT CATGATTACC ACAGAGACCT CTACAATCAC CTGGAGGAGC 
     3901 ACGAGATCGG CCCCAGCCTC TACGCTGCCC CCTGGTTCCT CACCATGTTT GCCTCACAGT 
     3961 TCCCGCTGGG ATTCGTAGCC AGAGTCTTTG ATATGATTTT TCTTCAGGGA ACAGAGGTCA 
     4021 TATTTAAAGT GGCTTTAAGT CTGTTGGGAA GCCATAAGCC CTTGATTCTG CAGCATGAAA 
     4081 ACCTAGAAAC CATAGTTGAC TTTATAAAAA GCACGCTACC CAACCTTGGC TTGGTACAGA 
     4141 TGGAAAAGAC CATCAATCAG GTATTTGAAA TGGACATCGC TAAACAGTTA CAAGCTTATG 
     4201 AAGTTGAGTA CCACGTCCTT CAAGAAGAAC TTATCGATTC CTCTCCTCTC AGTGACAACC 
     4261 AAAGAATGGA TAAATTAGAG AAAACCAACA GCAGCTTACG CAAACAGAAC CTTGACCTCC 
     4321 TTGAACAGTT GCAGGTGGCA AATGGTAGGA TCCAAAGCCT TGAGGCCACC ATTGAGAAGC 
     4381 TCCTGAGCAG TGAGAGCAAG CTGAAGCAGG CCATGCTTAC CTTAGAACTG GAGCGGTCGG 
     4441 CCCTGCTGCA GACGGTGGAG GAGCTGCGGC GGCGGAGCGC AGAGCCCAGC GACCGGGAGC 
     4501 CTGAGTGCAC GCAGCCCGAG CCCACGGGCG ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4561 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4621 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4681 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4741 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4801 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4861 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4921 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4981 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5041 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5101 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5161 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5221 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5281 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5341 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5401 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5461 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5521 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5581 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5641 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5701 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5761 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5821 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5881 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5941 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6001 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6061 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6121 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6181 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6241 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6301 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6361 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6421 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6481 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6541 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6601 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6661 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6721 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6781 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6841 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6901 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6961 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7021 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7081 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7141 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7201 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7261 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7321 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7381 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7441 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7501 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7561 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7621 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7801 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7861 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7921 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7981 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8041 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8101 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8161 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8221 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8281 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8341 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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