LOCUS       RC207917                8628 bp    DNA     circular  SYN 
DEFINITION  TPP2 (Myc-DDK-tagged)-Human tripeptidyl peptidase II (TPP2)
ACCESSION   NM_003291
VERSION     NM_003291.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="TPP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCGCT GCGACTGAGG AGCCCTTCCC TTTTCACGGT CTCCTGCCGA 
     1081 AGAAGGAGAC CGGAGCCGCC TCCTTCCTCT GCCGCTACCC GGAGTATGAT GGGCGGGGGG 
     1141 TGCTCATCGC AGTCCTGGAC ACGGGGGTCG ACCCGGGGGC TCCGGGCATG CAGGTTACAA 
     1201 CTGATGGAAA ACCAAAAATC GTTGATATCA TTGATACAAC AGGAAGTGGC GATGTGAATA 
     1261 CTGCTACAGA AGTAGAGCCA AAGGATGGTG AGATTGTTGG CCTTTCAGGA AGAGTGCTTA 
     1321 AGATTCCTGC AAGCTGGACA AATCCCTCAG GCAAATATCA TATTGGCATA AAAAATGGCT 
     1381 ATGACTTCTA TCCTAAGGCA CTCAAGGAAA GGATACAGAA AGAACGGAAG GAAAAAATCT 
     1441 GGGACCCTGT TCACAGAGTG GCCCTTGCAG AAGCCTGTAG AAAACAGGAA GAATTTGATG 
     1501 TTGCCAACAA CGGCTCTTCT CAAGCAAATA AACTAATCAA GGAGGAACTT CAAAGTCAAG 
     1561 TGGAATTGCT AAATTCTTTT GAGAAGAAAT ACAGCGATCC TGGCCCTGTA TATGACTGCT 
     1621 TGGTATGGCA TGATGGCGAA GTCTGGAGAG CCTGCATTGA TTCTAATGAA GATGGGGACT 
     1681 TGAGTAAATC TACCGTGTTG AGAAACTACA AAGAAGCCCA AGAATATGGC TCTTTTGGCA 
     1741 CAGCTGAGAT GTTGAATTAC TCCGTTAATA TATACGATGA TGGAAACCTG CTCTCCATTG 
     1801 TGACCAGCGG AGGAGCTCAT GGGACACATG TAGCTAGTAT AGCTGCTGGA CACTTTCCAG 
     1861 AAGAACCTGA ACGGAATGGG GTAGCTCCTG GTGCTCAAAT TCTTTCCATC AAGATTGGTG 
     1921 ATACAAGACT AAGCACAATG GAAACAGGCA CAGGCCTCAT AAGAGCTATG ATAGAAGTTA 
     1981 TAAATCATAA GTGTGATCTT GTCAACTACA GTTACGGAGA AGCAACTCAC TGGCCAAATT 
     2041 CTGGGAGAAT TTGTGAAGTA ATTAATGAAG CAGTATGGAA GCATAATATA ATTTATGTTT 
     2101 CAAGTGCTGG AAATAATGGT CCATGCCTGT CTACAGTTGG TTGTCCAGGT GGAACTACAT 
     2161 CAAGTGTGAT AGGTGTTGGT GCTTATGTTT CTCCTGATAT GATGGTTGCT GAGTATTCAC 
     2221 TGAGAGAGAA ATTACCTGCA AATCAATATA CTTGGTCTTC TAGAGGACCT AGTGCTGACG 
     2281 GGGCCCTTGG TGTGAGTATC AGTGCGCCAG GAGGAGCCAT TGCTTCTGTT CCTAACTGGA 
     2341 CACTGAGAGG GACGCAGCTG ATGAATGGAA CATCTATGTC TTCCCCCAAT GCATGTGGAG 
     2401 GCATTGCCCT GATCCTTTCA GGTCTGAAAG CTAATAACAT TGACTACACA GTTCATTCAG 
     2461 TCAGAAGAGC TCTAGAAAAC ACTGCAGTGA AGGCTGACAA TATAGAAGTA TTTGCTCAAG 
     2521 GACATGGTAT TATTCAGGTT GATAAAGCCT ATGACTACCT CGTTCAGAAT ACATCATTTG 
     2581 CTAATAAATT AGGTTTTACT GTTACTGTTG GAAATAACCG TGGCATCTAC CTCCGAGATC 
     2641 CTGTTCAGGT GGCTGCACCT TCAGATCATG GCGTTGGCAT TGAACCTGTA TTTCCGGAGA 
     2701 ACACAGAAAA CTCTGAAAAA ATATCCCTTC AGCTTCATTT AGCTCTGACT TCAAATTCAT 
     2761 CTTGGGTTCA GTGTCCCAGC CATTTGGAAC TCATGAATCA ATGTAGACAC ATAAACATAC 
     2821 GTGTGGATCC CAGGGGCTTA AGAGAAGGAT TGCATTATAC AGAGGTATGT GGCTATGATA 
     2881 TAGCATCCCC TAACGCAGGT CCGCTCTTCA GAGTTCCGAT CACTGCAGTT ATAGCAGCAA 
     2941 AAGTAAATGA ATCATCACAT TATGATCTAG CCTTTACAGA TGTACACTTT AAACCTGGTC 
     3001 AAATTCGAAG GCATTTTATT GAGGTTCCTG AGGGTGCAAC ATGGGCTGAA GTGACAGTGT 
     3061 GTTCGTGTTC TTCTGAGGTG TCAGCAAAGT TTGTTCTACA TGCAGTCCAG CTTGTGAAGC 
     3121 AAAGAGCATA TCGAAGCCAT GAATTCTATA AGTTTTGTTC TCTTCCAGAG AAAGGAACAC 
     3181 TGACTGAAGC TTTTCCTGTC CTAGGTGGAA AAGCAATTGA ATTTTGCATT GCTCGTTGGT 
     3241 GGGCAAGTCT CAGTGATGTC AACATTGATT ATACCATTTC TTTCCATGGG ATAGTGTGTA 
     3301 CTGCTCCTCA GTTAAACATT CATGCATCGG AAGGAATCAA CCGCTTTGAT GTTCAGTCCT 
     3361 CCTTGAAATA CGAAGATCTG GCTCCCTGCA TAACTTTGAA GAACTGGGTC CAAACACTGC 
     3421 GCCCAGTGAG TGCAAAAACA AAACCTTTAG GATCAAGAGA TGTTTTGCCA AATAACCGTC 
     3481 AACTTTATGA GATGGTCCTG ACATATAACT TTCATCAACC CAAGAGTGGG GAAGTAACTC 
     3541 CAAGCTGCCC ACTACTTTGT GAACTATTAT ATGAATCTGA ATTTGACAGC CAACTGTGGA 
     3601 TTATTTTTGA CCAGAACAAA AGACAGATGG GTTCAGGCGA TGCCTATCCA CATCAGTATT 
     3661 CTTTGAAACT GGAGAAAGGA GATTATACAA TTCGACTACA GATTCGCCAT GAGCAAATCA 
     3721 GTGATTTGGA ACGCCTTAAA GACCTTCCAT TTATTGTTTC TCATAGATTG TCTAATACCT 
     3781 TGAGCTTAGA TATTCATGAA AATCATAGTT TTGCACTTCT AGGGAAGAAG AAATCAAGCA 
     3841 ATTTGACATT ACCACCCAAA TATAACCAGC CATTCTTTGT TACTTCCTTA CCTGATGATA 
     3901 AAATACCTAA AGGGGCAGGA CCTGGATGCT ATCTTGCAGG ATCCTTAACA TTGTCAAAGA 
     3961 CTGAACTAGG AAAGAAAGCT GATGTAATCC CTGTTCATTA CTACTTAATA CCTCCACCAA 
     4021 CAAAGACTAA GAATGGCAGC AAAGATAAGG AAAAAGATTC AGAAAAAGAG AAAGATTTAA 
     4081 AAGAAGAGTT TACTGAAGCA TTACGAGATC TTAAAATTCA GTGGATGACA AAGCTGGATT 
     4141 CTAGTGACAT TTATAACGAA TTGAAAGAAA CATATCCTAA TTATCTTCCT CTGTACGTTG 
     4201 CACGACTTCA TCAATTGGAT GCTGAAAAGG AACGAATGAA AAGACTTAAT GAAATTGTTG 
     4261 ATGCGGCAAA TGCTGTTATT TCTCATATAG ATCAAACAGC CCTAGCAGTT TATATTGCAA 
     4321 TGAAGACTGA TCCCAGGCCT GATGCAGCTA CTATAAAAAA TGACATGGAC AAACAAAAAT 
     4381 CCACCCTCGT AGATGCCCTT TGTAGGAAAG GTTGTGCCCT GGCAGACCAT CTTCTTCACA 
     4441 CCCAGGCTCA AGACGGAGCC ATTTCCACTG ATGCAGAAGG AAAGGAGGAG GAAGGAGAAA 
     4501 GTCCTTTGGA TTCTCTGGCA GAAACATTTT GGGAAACTAC TAAATGGACT GATCTCTTTG 
     4561 ACAATAAGGT TTTGACATTT GCATATAAAC ATGCATTAGT AAATAAAATG TATGGGAGAG 
     4621 GCCTTAAATT TGCAACTAAA CTTGTGGAAG AAAAACCAAC AAAAGAAAAC TGGAAAAATT 
     4681 GTATTCAACT GATGAAGTTA CTTGGATGGA CCCATTGTGC ATCTTTTACT GAAAACTGGC 
     4741 TCCCCATCAT GTATCCTCCC GATTATTGCG TATTCACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//