LOCUS       RC207790                8187 bp    DNA     circular  SYN 
DEFINITION  PIK3CG (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, catalytic, gamma polypeptide (PIK3CG)
ACCESSION   NM_002649
VERSION     NM_002649.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4344..4435 
                     /label="Myc-DDK"  
     misc_feature    4465..5050 
                     /label="PolyA signal"  
     misc_feature    4522..4541 
                     /label="Restriction Enzyme"  
     misc_feature    5326..5998 
                     /label="ColE1"  
     misc_feature    6428..7222 
                     /label="Kan/Neo"  
     misc_feature    7257..7559 
                     /label="SV40 ori"  
     misc_feature    7645..8122 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4326..4332 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4326 
                     /label="PIK3CG"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGGAG AACTATAAAC AGCCCGTGGT GCTGAGAGAG GACAACTGCC 
     1081 GAAGGCGCCG GAGGATGAAG CCGCGCAGTG CTGCGGCCAG CCTGTCCTCC ATGGAGCTCA 
     1141 TCCCCATCGA GTTCGTGCTG CCCACCAGCC AGCGCAAATG CAAGAGCCCC GAAACGGCGC 
     1201 TGCTGCACGT GGCCGGCCAC GGCAACGTGG AGCAGATGAA GGCCCAGGTG TGGCTGCGAG 
     1261 CGCTGGAGAC CAGCGTGGCG GCGGACTTCT ACCACCGGCT GGGACCGCAT CACTTCCTCC 
     1321 TGCTCTATCA GAAGAAGGGG CAGTGGTACG AGATCTACGA CAAGTACCAG GTGGTGCAGA 
     1381 CTCTGGACTG CCTGCGCTAC TGGAAGGCCA CGCACCGGAG CCCGGGCCAG ATCCACCTGG 
     1441 TGCAGCGGCA CCCGCCCTCC GAGGAGTCCC AAGCCTTCCA GCGGCAGCTC ACGGCGCTGA 
     1501 TTGGCTATGA CGTCACTGAC GTCAGCAACG TGCACGACGA TGAGCTGGAG TTCACGCGCC 
     1561 GTGGCTTGGT GACCCCGCGC ATGGCGGAGG TGGCCAGCCG CGACCCCAAG CTCTACGCCA 
     1621 TGCACCCGTG GGTGACGTCC AAGCCCCTCC CGGAGTACCT GTGGAAGAAG ATTGCCAACA 
     1681 ACTGCATCTT CATCGTCATT CACCGCAGCA CCACCAGCCA GACCATTAAG GTCTCACCCG 
     1741 ACGACACCCC CGGCGCCATC CTGCAGAGCT TCTTCACCAA GATGGCCAAG AAGAAATCTC 
     1801 TGATGGATAT TCCCGAAAGC CAAAGCGAAC AGGATTTTGT GCTGCGCGTC TGTGGCCGGG 
     1861 ATGAGTACCT GGTGGGCGAA ACGCCCATCA AAAACTTCCA GTGGGTGAGG CACTGCCTCA 
     1921 AGAACGGAGA AGAGATTCAC GTGGTACTGG ACACGCCTCC AGACCCGGCC CTAGACGAGG 
     1981 TGAGGAAGGA AGAGTGGCCG CTGGTGGACG ACTGCACGGG AGTCACCGGC TACCATGAGC 
     2041 AGCTTACCAT CCACGGCAAG GACCACGAGA GTGTGTTCAC CGTGTCCCTG TGGGACTGCG 
     2101 ACCGCAAGTT CAGGGTCAAG ATCAGAGGCA TTGATATCCC CGTCCTGCCT CGGAACACCG 
     2161 ACCTCACAGT TTTTGTAGAG GCAAACATCC AGCATGGGCA ACAAGTCCTT TGCCAAAGGA 
     2221 GAACCAGCCC CAAACCCTTC ACAGAGGAGG TGCTGTGGAA TGTGTGGCTT GAGTTCAGTA 
     2281 TCAAAATCAA AGACTTGCCC AAAGGGGCTC TACTGAACCT CCAGATCTAC TGCGGTAAAG 
     2341 CTCCAGCACT GTCCAGCAAG GCCTCTGCAG AGTCCCCCAG TTCTGAGTCC AAGGGCAAAG 
     2401 TTCAGCTTCT CTATTATGTG AACCTGCTGC TGATAGACCA CCGTTTCCTC CTGCGCCGTG 
     2461 GAGAATACGT CCTCCACATG TGGCAGATAT CTGGGAAGGG AGAAGACCAA GGAAGCTTCA 
     2521 ATGCTGACAA ACTCACGTCT GCAACTAACC CAGACAAGGA GAACTCAATG TCCATCTCCA 
     2581 TTCTTCTGGA CAATTACTGC CACCCGATAG CCCTGCCTAA GCATCAGCCC ACCCCTGACC 
     2641 CGGAAGGGGA CCGGGTTCGA GCAGAAATGC CCAACCAGCT TCGCAAGCAA TTGGAGGCGA 
     2701 TCATAGCCAC TGATCCACTT AACCCTCTCA CAGCAGAGGA CAAAGAATTG CTCTGGCATT 
     2761 TTAGATACGA AAGCCTTAAG CACCCAAAAG CATATCCTAA GCTATTTAGT TCAGTGAAAT 
     2821 GGGGACAGCA AGAAATTGTG GCCAAAACAT ACCAATTGTT GGCCAGAAGG GAAGTCTGGG 
     2881 ATCAAAGTGC TTTGGATGTT GGGTTAACAA TGCAGCTCCT GGACTGCAAC TTCTCAGATG 
     2941 AAAATGTAAG AGCCATTGCA GTTCAGAAAC TGGAGAGCTT GGAGGACGAT GATGTTCTGC 
     3001 ATTACCTTCT ACAATTGGTC CAGGCTGTGA AATTTGAACC ATACCATGAT AGCGCCCTTG 
     3061 CCAGATTTCT GCTGAAGCGT GGTTTAAGAA ACAAAAGAAT TGGTCACTTT TTGTTTTGGT 
     3121 TCTTGAGAAG TGAGATAGCC CAGTCCAGAC ACTATCAGCA GAGGTTCGCT GTGATTCTGG 
     3181 AAGCCTATCT GAGGGGCTGT GGCACAGCCA TGCTGCACGA CTTTACCCAA CAAGTCCAAG 
     3241 TAATCGAGAT GTTACAAAAA GTCACCCTTG ATATTAAATC GCTCTCTGCT GAAAAGTATG 
     3301 ACGTCAGTTC CCAAGTTATT TCACAACTTA AACAAAAGCT TGAAAACCTG CAGAATTCTC 
     3361 AACTCCCCGA AAGCTTTAGA GTTCCATATG ATCCTGGACT GAAAGCAGGA GCGCTGGCAA 
     3421 TTGAAAAATG TAAAGTAATG GCCTCCAAGA AAAAACCACT ATGGCTTGAG TTTAAATGTG 
     3481 CCGATCCTAC AGCCCTATCA AATGAAACAA TTGGAATTAT CTTTAAACAT GGTGATGATC 
     3541 TGCGCCAAGA CATGCTTATT TTACAGATTC TACGAATCAT GGAGTCTATT TGGGAGACTG 
     3601 AATCTTTGGA TCTATGCCTC CTGCCATATG GTTGCATTTC AACTGGTGAC AAAATAGGAA 
     3661 TGATCGAGAT TGTGAAAGAC GCCACGACAA TTGCCAAAAT TCAGCAAAGC ACAGTGGGCA 
     3721 ACACGGGAGC ATTTAAAGAT GAAGTCCTGA ATCACTGGCT CAAAGAAAAA TCCCCTACTG 
     3781 AAGAAAAGTT TCAGGCAGCA GTGGAGAGAT TTGTTTATTC CTGTGCAGGC TACTGTGTGG 
     3841 CAACCTTTGT TCTTGGAATA GGCGACAGAC ACAATGACAA TATTATGATC ACCGAGACAG 
     3901 GAAACCTATT TCATATTGAC TTCGGGCACA TTCTTGGGAA TTACAAAAGT TTCCTGGGCA 
     3961 TTAATAAAGA GAGAGTGCCA TTTGTGCTAA CCCCTGACTT CCTCTTTGTG ATGGGAACTT 
     4021 CTGGAAAGAA GACAAGCCCA CACTTCCAGA AATTTCAGGA CATCTGTGTT AAGGCTTATC 
     4081 TAGCCCTTCG TCATCACACA AACCTACTGA TCATCCTGTT CTCCATGATG CTGATGACAG 
     4141 GAATGCCCCA GTTAACAAGC AAAGAAGACA TTGAATATAT CCGGGATGCC CTCACAGTGG 
     4201 GGAAAAATGA GGAGGATGCT AAAAAGTATT TTCTTGATCA GATCGAAGTT TGCAGAGACA 
     4261 AAGGATGGAC TGTGCAGTTT AATTGGTTTC TACATCTTGT TCTTGGCATC AAACAAGGAG 
     4321 AGAAACATTC AGCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4381 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4441 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4501 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4561 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4621 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4681 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4741 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4801 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4861 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4921 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4981 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5041 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5101 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5161 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5221 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5281 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5341 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5401 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5461 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5521 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5581 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5641 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5701 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5761 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5821 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5881 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5941 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6001 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6061 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6121 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6181 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6241 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6301 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6361 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6421 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6481 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6541 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6601 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6661 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6721 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6781 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6841 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6901 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6961 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7021 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7081 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7141 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7201 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7261 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7321 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7381 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7441 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7501 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7621 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7681 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7741 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7801 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7861 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7921 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7981 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8041 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8101 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8161 ATTTAACAAA AATTTAACGC GAATTTT
//