LOCUS       RC207788L1                9201 bp    DNA     circular  SYN 
DEFINITION  PKN1 (Myc-DDK-tagged)-Human protein kinase N1 (PKN1), transcript variant 2
ACCESSION   NM_002741
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7492..7672 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8400..9059 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7831..8399 
                     /label="ColE1"  
     misc_feature    6367..6387 
                     /label="LR50 primer"  
     misc_feature    6164..6235 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6376..6967 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6146..6152 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6146 
                     /label="PKN1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCAGCGAC GCCGTGCAGA GTGAGCCTCG 
     3361 CAGCTGGTCC CTGCTAGAGC AGCTGGGCCT GGCCGGGGCA GACCTGGCGG CCCCCGGGGT 
     3421 ACAGCAGCAG CTGGAGCTGG AGCGGGAGCG GCTGCGGCGG GAAATCCGCA AGGAGCTGAA 
     3481 GCTGAAGGAG GGTGCTGAGA ACCTGCGGCG GGCCACCACT GACCTGGGCC GCAGCCTGGG 
     3541 CCCCGTAGAG CTGCTGCTGC GGGGCTCCTC GCGCCGCCTC GACCTGCTGC ACCAGCAGCT 
     3601 GCAGGAGCTG CACGCCCACG TGGTGCTTCC CGACCCGGCG GCCACCCACG ATGGCCCCCA 
     3661 GTCCCCTGGT GCGGGTGGCC CCACCTGCTC GGCCACCAAC CTGAGCCGCG TGGCGGGCCT 
     3721 GGAGAAGCAG TTGGCCATTG AGCTGAAGGT GAAGCAGGGG GCGGAGAACA TGATCCAGAC 
     3781 CTACAGCAAT GGCAGCACCA AGGACCGGAA GCTGCTGCTG ACAGCCCAGC AGATGTTGCA 
     3841 GGACAGTAAG ACCAAGATTG ACATCATCCG CATGCAACTC CGCCGGGCGC TGCAGGCCGG 
     3901 CCAGCTGGAG AACCAGGCAG CCCCGGATGA CACCCAAGGG AGTCCTGACC TGGGGGCTGT 
     3961 GGAGCTGCGC ATCGAAGAGC TGCGGCACCA CTTCCGAGTG GAGCACGCGG TGGCCGAGGG 
     4021 TGCCAAGAAC GTACTGCGCC TGCTCAGCGC TGCCAAGGCC CCGGACCGCA AGGCAGTCAG 
     4081 CGAGGCCCAG GAGAAATTGA CAGAATCCAA CCAGAAGCTG GGGCTGCTGC GGGAGGCTCT 
     4141 GGAGCGGAGA CTTGGGGAGC TGCCCGCCGA CCACCCCAAG GGGCGGCTGC TGCGAGAAGA 
     4201 GCTCGCTGCG GCCTCCTCCG CTGCCTTCAG CACCCGCCTG GCCGGGCCCT TTCCCGCCAC 
     4261 GCACTACAGC ACCCTGTGCA AGCCCGCGCC GCTCACAGGG ACCCTGGAGG TACGAGTGGT 
     4321 GGGCTGCAGA GACCTCCCAG AGACCATCCC GTGGAACCCT ACCCCCTCAA TGGGGGGACC 
     4381 TGGGACCCCA GACAGCCGCC CCCCCTTCCT GAGCCGCCCA GCCCGGGGCC TTTACAGCCG 
     4441 AAGCGGAAGC CTCAGTGGCC GGAGCAGCCT CAAAGCAGAA GCCGAGAACA CCAGTGAAGT 
     4501 CAGCACTGTG CTTAAGCTGG ATAACACAGT GGTGGGGCAG ACGTCTTGGA AGCCATGTGG 
     4561 CCCCAATGCC TGGGACCAGA GCTTCACTCT GGAGCTGGAA AGGGCACGGG AACTGGAGTT 
     4621 GGCTGTGTTC TGGCGGGACC AGCGGGGCCT GTGTGCCCTC AAATTCCTGA AGTTGGAGGA 
     4681 TTTCTTGGAC AATGAGAGGC ATGAGGTGCA GCTGGACATG GAACCCCAGG GCTGCCTGGT 
     4741 GGCTGAGGTC ACCTTCCGCA ACCCTGTCAT TGAGAGGATT CCTCGGCTCC GACGGCAGAA 
     4801 GAAAATTTTC TCCAAGCAGC AAGGGAAGGC GTTCCAGCGT GCTAGGCAGA TGAACATCGA 
     4861 TGTCGCCACG TGGGTGCGGC TGCTCCGGAG GCTCATCCCC AATGCCACGG GCACAGGCAC 
     4921 CTTTAGCCCT GGGGCTTCTC CAGGATCCGA GGCCCGGACC ACGGGTGACA TATCGGTGGA 
     4981 GAAGCTGAAC CTCGGCACTG ACTCGGACAG CTCACCTCAG AAGAGCTCGC GGGATCCTCC 
     5041 TTCCAGCCCA TCGAGCCTGA GCTCCCCCAT CCAGGAATCC ACTGCTCCCG AGCTGCCTTC 
     5101 GGAGACCCAG GAGACCCCAG GCCCCGCCCT GTGCAGCCCT CTGAGGAAGT CACCTCTGAC 
     5161 CCTCGAAGAT TTCAAGTTCC TGGCGGTGCT GGGCCGGGGT CATTTTGGGA AGGTGCTCCT 
     5221 CTCCGAATTC CGGCCCAGTG GGGAGCTGTT CGCCATCAAG GCTCTGAAGA AAGGGGACAT 
     5281 TGTGGCCCGA GACGAGGTGG AGAGCCTGAT GTGTGAGAAG CGGATATTGG CGGCAGTGAC 
     5341 CAGTGCGGGA CACCCCTTCC TGGTGAACCT CTTCGGCTGT TTCCAGACAC CGGAGCACGT 
     5401 GTGCTTCGTG ATGGAGTACT CGGCCGGTGG GGACCTGATG CTGCACATCC ACAGCGACGT 
     5461 GTTCTCTGAG CCCCGTGCCA TCTTTTATTC CGCCTGCGTG GTGCTGGGCC TACAGTTTCT 
     5521 TCACGAACAC AAGATCGTCT ACAGGGACCT GAAGTTGGAC AATTTGCTCC TGGACACCGA 
     5581 GGGCTACGTC AAGATCGCAG ACTTTGGCCT CTGCAAGGAG GGGATGGGCT ATGGGGACCG 
     5641 GACCAGCACA TTCTGTGGGA CCCCGGAGTT CCTGGCCCCT GAGGTGCTGA CGGACACGTC 
     5701 GTACACGCGA GCTGTGGACT GGTGGGGACT GGGTGTGCTG CTCTACGAGA TGCTGGTTGG 
     5761 CGAGTCCCCA TTCCCAGGGG ATGATGAGGA GGAGGTCTTC GACAGCATCG TCAACGACGA 
     5821 GGTTCGCTAC CCCCGCTTCC TGTCGGCCGA AGCCATCGGC ATCATGAGAA GGCTGCTTCG 
     5881 GAGGAACCCA GAGCGGAGGC TGGGATCTAG CGAGAGAGAT GCAGAAGATG TGAAGAAACA 
     5941 GCCCTTCTTC AGGACTCTGG GCTGGGAAGC CCTGTTGGCC CGGCGCCTGC CACCGCCCTT 
     6001 TGTGCCCACG CTGTCCGGCC GCACCGACAT CAGCAACTTC GACGAGGAGT TCACCGGGGA 
     6061 GGCCCCCACA CTGAGCCCGC CCCGCGACGC GCGGCCCCTC ACAGCCGCGG AGCAGGCAGC 
     6121 CTTCCTGGAC TTCGACTTCG TGGCCGGGGG CTGCACGCGT ACGCGGCCGC TCGAGCAGAA 
     6181 ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA 
     6241 TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA CAAGTAGGAT TCGTCGAGGG ACCTAATAAC 
     6301 TTCGTATAGC ATACATTATA CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA 
     6361 CAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 
     6421 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 
     6481 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 
     6541 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 
     6601 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 
     6661 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 
     6721 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA 
     6781 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 
     6841 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 
     6901 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 
     6961 GCCGCCTCCC CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT 
     7021 CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA 
     7081 GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC 
     7141 AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC 
     7201 CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA 
     7261 TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA 
     7321 CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG 
     7381 CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG 
     7441 GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC 
     7501 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC 
     7561 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 
     7621 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 
     7681 CAGCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     7741 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     7801 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     7861 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     7921 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     7981 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     8041 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     8101 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     8161 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     8221 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     8281 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     8341 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     8401 GGTCATGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT 
     8461 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC 
     8521 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC 
     8581 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA 
     8641 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA 
     8701 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT 
     8761 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC 
     8821 ACCAGCTCAC CGTCTTTCAT TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA 
     8881 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG 
     8941 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC 
     9001 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT 
     9061 TTCTCCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     9121 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTCCC GCGCACATTT 
     9181 CCCCGAAAAG TGCCACCTGA C
//