LOCUS       RC207771                8295 bp    DNA     circular  SYN 
DEFINITION  TIE1 (Myc-DDK-tagged)-Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)
ACCESSION   NM_005424
VERSION     NM_005424.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4452..4543 
                     /label="Myc-DDK"  
     misc_feature    4573..5158 
                     /label="PolyA signal"  
     misc_feature    4630..4649 
                     /label="Restriction Enzyme"  
     misc_feature    5434..6106 
                     /label="ColE1"  
     misc_feature    6536..7330 
                     /label="Kan/Neo"  
     misc_feature    7365..7667 
                     /label="SV40 ori"  
     misc_feature    7753..8230 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4434..4440 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4434 
                     /label="TIE1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCTGGCGG GTGCCCCCTT TCTTGCTCCC CATCCTCTTC TTGGCTTCTC 
     1081 ATGTGGGCGC GGCGGTGGAC CTGACGCTGC TGGCCAACCT GCGGCTCACG GACCCCCAGC 
     1141 GCTTCTTCCT GACTTGCGTG TCTGGGGAGG CCGGGGCGGG GAGGGGCTCG GACGCCTGGG 
     1201 GCCCGCCCCT GCTGCTGGAG AAGGACGACC GTATCGTGCG CACCCCGCCC GGGCCACCCC 
     1261 TGCGCCTGGC GCGCAACGGT TCGCACCAGG TCACGCTTCG CGGCTTCTCC AAGCCCTCGG 
     1321 ACCTCGTGGG CGTCTTCTCC TGCGTGGGCG GTGCTGGGGC GCGGCGCACG CGCGTCATCT 
     1381 ACGTGCACAA CAGCCCTGGA GCCCACCTGC TTCCAGACAA GGTCACACAC ACTGTGAACA 
     1441 AAGGTGACAC CGCTGTACTT TCTGCACGTG TGCACAAGGA GAAGCAGACA GACGTGATCT 
     1501 GGAAGAGCAA CGGATCCTAC TTCTACACCC TGGACTGGCA TGAAGCCCAG GATGGGCGGT 
     1561 TCCTGCTGCA GCTCCCAAAT GTGCAGCCAC CATCGAGCGG CATCTACAGT GCCACTTACC 
     1621 TGGAAGCCAG CCCCCTGGGC AGCGCCTTCT TTCGGCTCAT CGTGCGGGGT TGTGGGGCTG 
     1681 GGCGCTGGGG GCCAGGCTGT ACCAAGGAGT GCCCAGGTTG CCTACATGGA GGTGTCTGCC 
     1741 ACGACCATGA CGGCGAATGT GTATGCCCCC CTGGCTTCAC TGGCACCCGC TGTGAACAGG 
     1801 CCTGCAGAGA GGGCCGTTTT GGGCAGAGCT GCCAGGAGCA GTGCCCAGGC ATATCAGGCT 
     1861 GCCGGGGCCT CACCTTCTGC CTCCCAGACC CCTATGGCTG CTCTTGTGGA TCTGGCTGGA 
     1921 GAGGAAGCCA GTGCCAAGAA GCTTGTGCCC CTGGTCATTT TGGGGCTGAT TGCCGACTCC 
     1981 AGTGCCAGTG TCAGAATGGT GGCACTTGTG ACCGGTTCAG TGGTTGTGTC TGCCCCTCTG 
     2041 GGTGGCATGG AGTGCACTGT GAGAAGTCAG ACCGGATCCC CCAGATCCTC AACATGGCCT 
     2101 CAGAACTGGA GTTCAACTTA GAGACGATGC CCCGGATCAA CTGTGCAGCT GCAGGGAACC 
     2161 CCTTCCCCGT GCGGGGCAGC ATAGAGCTAC GCAAGCCAGA CGGCACTGTG CTCCTGTCCA 
     2221 CCAAGGCCAT TGTGGAGCCA GAGAAGACCA CAGCTGAGTT CGAGGTGCCC CGCTTGGTTC 
     2281 TTGCGGACAG TGGGTTCTGG GAGTGCCGTG TGTCCACATC TGGCGGCCAA GACAGCCGGC 
     2341 GCTTCAAGGT CAATGTGAAA GTGCCCCCCG TGCCCCTGGC TGCACCTCGG CTCCTGACCA 
     2401 AGCAGAGCCG CCAGCTTGTG GTCTCCCCGC TGGTCTCGTT CTCTGGGGAT GGACCCATCT 
     2461 CCACTGTCCG CCTGCACTAC CGGCCCCAGG ACAGTACCAT GGACTGGTCG ACCATTGTGG 
     2521 TGGACCCCAG TGAGAACGTG ACGTTAATGA ACCTGAGGCC AAAGACAGGA TACAGTGTTC 
     2581 GTGTGCAGCT GAGCCGGCCA GGGGAAGGAG GAGAGGGGGC CTGGGGGCCT CCCACCCTCA 
     2641 TGACCACAGA CTGTCCTGAG CCTTTGTTGC AGCCGTGGTT GGAGGGCTGG CATGTGGAAG 
     2701 GCACTGACCG GCTGCGAGTG AGCTGGTCCT TGCCCTTGGT GCCCGGGCCA CTGGTGGGCG 
     2761 ACGGTTTCCT GCTGCGCCTG TGGGACGGGA CACGGGGGCA GGAGCGGCGG GAGAACGTCT 
     2821 CATCCCCCCA GGCCCGCACT GCCCTCCTGA CGGGACTCAC GCCTGGCACC CACTACCAGC 
     2881 TGGATGTGCA GCTCTACCAC TGCACCCTCC TGGGCCCGGC CTCGCCCCCT GCACACGTGC 
     2941 TTCTGCCCCC CAGTGGGCCT CCAGCCCCCC GACACCTCCA CGCCCAGGCC CTCTCAGACT 
     3001 CCGAGATCCA GCTGACATGG AAGCACCCGG AGGCTCTGCC TGGGCCAATA TCCAAGTACG 
     3061 TTGTGGAGGT GCAGGTGGCT GGGGGTGCAG GAGACCCACT GTGGATAGAC GTGGACAGGC 
     3121 CTGAGGAGAC AAGCACCATC ATCCGTGGCC TCAACGCCAG CACGCGCTAC CTCTTCCGCA 
     3181 TGCGGGCCAG CATTCAGGGG CTCGGGGACT GGAGCAACAC AGTAGAAGAG TCCACCCTGG 
     3241 GCAACGGGCT GCAGGCTGAG GGCCCAGTCC AAGAGAGCCG GGCAGCTGAA GAGGGCCTGG 
     3301 ATCAGCAGCT GATCCTGGCG GTGGTGGGCT CCGTGTCTGC CACCTGCCTC ACCATCCTGG 
     3361 CCGCCCTTTT AACCCTGGTG TGCATCCGCA GAAGCTGCCT GCATCGGAGA CGCACCTTCA 
     3421 CCTACCAGTC AGGCTCGGGC GAGGAGACCA TCCTGCAGTT CAGCTCAGGG ACCTTGACAC 
     3481 TTACCCGGCG GCCAAAACTG CAGCCCGAGC CCCTGAGCTA CCCAGTGCTA GAGTGGGAGG 
     3541 ACATCACCTT TGAGGACCTC ATCGGGGAGG GGAACTTCGG CCAGGTCATC CGGGCCATGA 
     3601 TCAAGAAGGA CGGGCTGAAG ATGAACGCAG CCATCAAAAT GCTGAAAGAG TATGCCTCTG 
     3661 AAAATGACCA TCGTGACTTT GCGGGAGAAC TGGAAGTTCT GTGCAAATTG GGGCATCACC 
     3721 CCAACATCAT CAACCTCCTG GGGGCCTGTA AGAACCGAGG TTACTTGTAT ATCGCTATTG 
     3781 AATATGCCCC CTACGGGAAC CTGCTAGATT TTCTGCGGAA AAGCCGGGTC CTAGAGACTG 
     3841 ACCCAGCTTT TGCTCGAGAG CATGGGACAG CCTCTACCCT TAGCTCCCGG CAGCTGCTGC 
     3901 GTTTCGCCAG TGATGCGGCC AATGGCATGC AGTACCTGAG TGAGAAGCAG TTCATCCACA 
     3961 GGGACCTGGC TGCCCGGAAT GTGCTGGTCG GAGAGAACCT AGCCTCCAAG ATTGCAGACT 
     4021 TCGGCCTTTC TCGGGGAGAG GAGGTTTATG TGAAGAAGAC GATGGGGCGT CTCCCTGTGC 
     4081 GCTGGATGGC CATTGAGTCC CTGAACTACA GTGTCTATAC CACCAAGAGT GATGTCTGGT 
     4141 CCTTTGGAGT CCTTCTTTGG GAGATAGTGA GCCTTGGAGG TACACCCTAC TGTGGCATGA 
     4201 CCTGTGCCGA GCTCTATGAA AAGCTGCCCC AGGGCTACCG CATGGAGCAG CCTCGAAACT 
     4261 GTGACGATGA AGTGTACGAG CTGATGCGTC AGTGCTGGCG GGACCGTCCC TATGAGCGAC 
     4321 CCCCCTTTGC CCAGATTGCG CTACAGCTAG GCCGCATGCT GGAAGCCAGG AAGGCCTATG 
     4381 TGAACATGTC GCTGTTTGAG AACTTCACTT ACGCGGGCAT TGATGCCACA GCTGAGGAGG 
     4441 CCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4501 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4561 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4621 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4681 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4741 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4801 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4861 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4921 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4981 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5041 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5101 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5161 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5221 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5281 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5341 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5401 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5461 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5521 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5581 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5641 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5701 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5761 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5821 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5881 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5941 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6001 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6061 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6121 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6181 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6241 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6301 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6361 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6421 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6481 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6541 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6601 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6661 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6721 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6781 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6841 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6901 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6961 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7021 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7081 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7141 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7201 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7261 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7321 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7381 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7441 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7501 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7561 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7741 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7801 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7861 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7921 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7981 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8041 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8101 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8161 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8221 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8281 TTTAACGCGA ATTTT
//