LOCUS       RC207735                6888 bp    DNA     circular  SYN 
DEFINITION  GUF1 (Myc-DDK-tagged)-Human GUF1 GTPase homolog (S. cerevisiae) (GUF1)
ACCESSION   NM_021927
VERSION     NM_021927.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3045..3136 
                     /label="Myc-DDK"  
     misc_feature    3166..3751 
                     /label="PolyA signal"  
     misc_feature    3223..3242 
                     /label="Restriction Enzyme"  
     misc_feature    4027..4699 
                     /label="ColE1"  
     misc_feature    5129..5923 
                     /label="Kan/Neo"  
     misc_feature    5958..6260 
                     /label="SV40 ori"  
     misc_feature    6346..6823 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3027..3033 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3027 
                     /label="GUF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGACCCTC GTGGGTCGGG GCTGGGGGTG CGCACGCGCT CTCGCGCCAC 
     1081 GAGCCACTGG GGCCGCGCTT CTGGTGGCCC CGGGGCCCCG GTCCGCGCCG ACCCTTGGGG 
     1141 CTGCTCCAGA GTCCTGGGCT ACCGACAGGC TCTACAGCTC CGCAGAATTC AAGGAAAAAC 
     1201 TTGACATGTC TAGGTTTCCT GTTGAAAATA TTAGAAATTT CAGTATTGTT GCACACGTGG 
     1261 ATCATGGCAA AAGTACTTTA GCTGACAGGC TCCTAGAACT TACAGGGACA ATTGATAAAA 
     1321 CAAAGAATAA TAAGCAGGTT CTTGATAAAT TGCAAGTGGA ACGAGAAAGA GGAATCACTG 
     1381 TTAAAGCACA GACAGCATCT CTCTTTTACA ATTGTGAAGG AAAGCAGTAC CTTTTAAATC 
     1441 TCATTGATAC ACCGGGCCAT GTTGATTTTA GTTATGAAGT ATCCAGGTCA CTTTCTGCTT 
     1501 GCCAGGGTGT TTTACTTGTG GTTGATGCAA ATGAGGGAAT TCAAGCCCAA ACTGTAGCAA 
     1561 ACTTCTTTCT TGCCTTCGAA GCACAGCTAT CGGTAATTCC AGTTATAAAT AAGATAGATC 
     1621 TGAAGAATGC TGATCCTGAA AGGGTTGAAA ACCAAATTGA GAAAGTGTTT GATATTCCAA 
     1681 GTGATGAATG TATTAAGATT TCTGCTAAAC TTGGAACAAA TGTTGAGAGT GTTCTTCAGG 
     1741 CAATTATTGA AAGAATCCCC CCTCCTAAAG TGCATCGCAA AAATCCTCTG AGAGCTTTGG 
     1801 TATTTGACTC CACCTTTGAC CAGTATAGAG GTGTGATAGC CAATGTAGCA TTATTTGACG 
     1861 GAGTGGTTTC CAAAGGAGAT AAAATTGTAT CTGCACATAC TCAAAAGACA TACGAAGTTA 
     1921 ATGAAGTAGG AGTCTTGAAT CCTAATGAGC AGCCAACTCA TAAATTATAT GCAGGACAGG 
     1981 TGGGCTATCT GATTGCTGGG ATGAAAGATG TCACTGAAGC GCAAATAGGA GATACATTAT 
     2041 GTTTACATAA GCAACCAGTG GAGCCCTTGC CTGGGTTTAA ATCAGCGAAA CCAATGGTAT 
     2101 TTGCAGGAAT GTATCCTCTA GACCAATCTG AATATAACAA TCTGAAGAGT GCTATAGAAA 
     2161 AACTGACTTT AAATGATTCC AGTGTGACCG TTCATCGGGA TAGTAGCCTT GCTCTGGGTG 
     2221 CTGGCTGGAG GCTAGGATTT CTTGGACTTT TGCACATGGA AGTTTTCAAC CAGCGACTGG 
     2281 AGCAAGAATA TAATGCTTCT GTTATTTTAA CAACCCCTAC TGTTCCATAT AAAGCTGTAC 
     2341 TGTCATCATC AAAATTGATA AAGGAACATA GAGAAAAAGA AATTACAATT ATCAATCCTG 
     2401 CACAATTCCC CGATAAATCA AAAGTAACAG AATATTTGGA GCCAGTTGTT TTGGGCACTA 
     2461 TTATCACACC AGATGAATAC ACTGGAAAAA TAATGATGCT TTGCGAGGCT CGAAGAGCAG 
     2521 TTCAGAAGAA TATGATATTT ATTGATCAAA ATAGAGTTAT GCTTAAATAT CTCTTTCCTT 
     2581 TGAATGAAAT TGTGGTAGAT TTTTATGACT CTTTGAAATC CCTATCTTCT GGATATGCTA 
     2641 GTTTTGATTA CGAAGATGCA GGCTACCAGA CTGCAGAACT TGTAAAAATG GATATTCTAC 
     2701 TGAATGGAAA TACTGTAGAG GAGCTAGTAA CTGTTGTACA CAAAGACAAA GCTCATTCAA 
     2761 TTGGCAAAGC CATATGTGAA CGGCTGAAGG ATTCTCTTCC TAGGCAACTG TTTGAGATAG 
     2821 CAATTCAAGC TGCTATTGGA AGTAAAATCA TTGCAAGAGA AACTGTGAAA GCCTATAGGA 
     2881 AAAACGTTTT GGCAAAATGT TATGGTGGTG ATATTACCCG AAAAATGAAG CTTTTGAAGA 
     2941 GACAAGCAGA AGGGAAAAAA AAGCTGAGGA AAATTGGCAA CGTTGAAGTT CCAAAAGATG 
     3001 CTTTTATAAA AGTTCTGAAA ACACAATCTT CTAAAACGCG TACGCGGCCG CTCGAGCAGA 
     3061 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3121 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3181 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3241 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3301 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3361 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3421 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3481 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3541 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3601 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3661 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3721 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     3781 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     3841 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     3901 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     3961 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4021 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4081 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4141 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4201 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4261 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4321 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4381 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4441 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4501 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4561 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4621 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4681 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4741 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     4801 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     4861 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     4921 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     4981 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5041 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5101 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5161 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5221 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5281 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5341 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5401 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5461 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5521 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5581 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5641 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5701 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     5761 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     5821 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     5881 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     5941 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6001 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6061 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6121 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6181 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6241 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6301 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6361 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6421 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6481 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6541 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6601 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6661 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6721 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     6781 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     6841 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//