LOCUS       RC207731                7710 bp    DNA     circular  SYN 
DEFINITION  CLCA2 (Myc-DDK-tagged)-Human chloride channel accessory 2 (CLCA2)
ACCESSION   NM_006536
VERSION     NM_006536.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3867..3958 
                     /label="Myc-DDK"  
     misc_feature    3988..4573 
                     /label="PolyA signal"  
     misc_feature    4045..4064 
                     /label="Restriction Enzyme"  
     misc_feature    4849..5521 
                     /label="ColE1"  
     misc_feature    5951..6745 
                     /label="Kan/Neo"  
     misc_feature    6780..7082 
                     /label="SV40 ori"  
     misc_feature    7168..7645 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3849..3855 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3849 
                     /label="CLCA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCCAAAGG AGCATTGCAG GTCCTATTTG CAACCTGAAG TTTGTGACTC 
     1081 TCCTGGTTGC CTTAAGTTCA GAACTCCCAT TCCTGGGAGC TGGAGTACAG CTTCAAGACA 
     1141 ATGGGTATAA TGGATTGCTC ATTGCAATTA ATCCTCAGGT ACCTGAGAAT CAGAACCTCA 
     1201 TCTCAAACAT TAAGGAAATG ATAACTGAAG CTTCATTTTA CCTATTTAAT GCTACCAAGA 
     1261 GAAGAGTATT TTTCAGAAAT ATAAAGATTT TAATACCTGC CACATGGAAA GCTAATAATA 
     1321 ACAGCAAAAT AAAACAAGAA TCATATGAAA AGGCAAATGT CATAGTGACT GACTGGTATG 
     1381 GGGCACATGG AGATGATCCA TACACCCTAC AATACAGAGG GTGTGGAAAA GAGGGAAAAT 
     1441 ACATTCATTT CACACCTAAT TTCCTACTGA ATGATAACTT AACAGCTGGC TACGGATCAC 
     1501 GAGGCCGAGT GTTTGTCCAT GAATGGGCCC ACCTCCGTTG GGGTGTGTTC GATGAGTATA 
     1561 ACAATGACAA ACCTTTCTAC ATAAATGGGC AAAATCAAAT TAAAGTGACA AGGTGTTCAT 
     1621 CTGACATCAC AGGCATTTTT GTGTGTGAAA AAGGTCCTTG CCCCCAAGAA AACTGTATTA 
     1681 TTAGTAAGCT TTTTAAAGAA GGATGCACCT TTATCTACAA TAGCACCCAA AATGCAACTG 
     1741 CATCAATAAT GTTCATGCAA AGTTTATCTT CTGTGGTTGA ATTTTGTAAT GCAAGTACCC 
     1801 ACAACCAAGA AGCACCAAAC CTACAGAACC AGATGTGCAG CCTCAGAAGT GCATGGGATG 
     1861 TAATCACAGA CTCTGCTGAC TTTCACCACA GCTTTCCCAT GAATGGGACT GAGCTTCCAC 
     1921 CTCCTCCCAC ATTCTCGCTT GTACAGGCTG GTGACAAAGT GGTCTGTTTA GTGCTGGATG 
     1981 TGTCCAGCAA GATGGCAGAG GCTGACAGAC TCCTTCAACT ACAACAAGCC GCAGAATTTT 
     2041 ATTTGATGCA GATTGTTGAA ATTCATACCT TCGTGGGCAT TGCCAGTTTC GACAGCAAAG 
     2101 GAGAGATCAG AGCCCAGCTA CACCAAATTA ACAGCAATGA TGATCGAAAG TTGCTGGTTT 
     2161 CATATCTGCC CACCACTGTA TCAGCTAAAA CAGACATCAG CATTTGTTCA GGGCTTAAGA 
     2221 AAGGATTTGA GGTGGTTGAA AAACTGAATG GAAAAGCTTA TGGCTCTGTG ATGATATTAG 
     2281 TGACCAGCGG AGATGATAAG CTTCTTGGCA ATTGCTTACC CACTGTGCTC AGCAGTGGTT 
     2341 CAACAATTCA CTCCATTGCC CTGGGTTCAT CTGCAGCCCC AAATCTGGAG GAATTATCAC 
     2401 GTCTTACAGG AGGTTTAAAG TTCTTTGTTC CAGATATATC AAACTCCAAT AGCATGATTG 
     2461 ATGCTTTCAG TAGAATTTCC TCTGGAACTG GAGACATTTT CCAGCAACAT ATTCAGCTTG 
     2521 AAAGTACAGG TGAAAATGTC AAACCTCACC ATCAATTGAA AAACACAGTG ACTGTGGATA 
     2581 ATACTGTGGG CAACGACACT ATGTTTCTAG TTACGTGGCA GGCCAGTGGT CCTCCTGAGA 
     2641 TTATATTATT TGATCCTGAT GGACGAAAAT ACTACACAAA TAATTTTATC ACCAATCTAA 
     2701 CTTTTCGGAC AGCTAGTCTT TGGATTCCAG GAACAGCTAA GCCTGGGCAC TGGACTTACA 
     2761 CCCTGAACAA TACCCATCAT TCTCTGCAAG CCCTGAAAGT GACAGTGACC TCTCGCGCCT 
     2821 CCAACTCAGC TGTGCCCCCA GCCACTGTGG AAGCCTTTGT GGAAAGAGAC AGCCTCCATT 
     2881 TTCCTCATCC TGTGATGATT TATGCCAATG TGAAACAGGG ATTTTATCCC ATTCTTAATG 
     2941 CCACTGTCAC TGCCACAGTT GAGCCAGAGA CTGGAGATCC TGTTACGCTG AGACTCCTTG 
     3001 ATGATGGAGC AGGTGCTGAT GTTATAAAAA ATGATGGAAT TTACTCGAGG TATTTTTTCT 
     3061 CCTTTGCTGC AAATGGTAGA TATAGCTTGA AAGTGCATGT CAATCACTCT CCCAGCATAA 
     3121 GCACCCCAGC CCACTCTATT CCAGGGAGTC ATGCTATGTA TGTACCAGGT TACACAGCAA 
     3181 ACGGTAATAT TCAGATGAAT GCTCCAAGGA AATCAGTAGG CAGAAATGAG GAGGAGCGAA 
     3241 AGTGGGGCTT TAGCCGAGTC AGCTCAGGAG GCTCCTTTTC AGTGCTGGGA GTTCCAGCTG 
     3301 GCCCCCACCC TGATGTGTTT CCACCATGCA AAATTATTGA CCTGGAAGCT GTAAAAGTAG 
     3361 AAGAGGAATT GACCCTATCT TGGACAGCAC CTGGAGAAGA CTTTGATCAG GGCCAGGCTA 
     3421 CAAGCTATGA AATAAGAATG AGTAAAAGTC TACAGAATAT CCAAGATGAC TTTAACAATG 
     3481 CTATTTTAGT AAATACATCA AAGCGAAATC CTCAGCAAGC TGGCATCAGG GAGATATTTA 
     3541 CGTTCTCACC CCAAATTTCC ACGAATGGAC CTGAACATCA GCCAAATGGA GAAACACATG 
     3601 AAAGCCACAG AATTTATGTT GCAATACGAG CAATGGATAG GAACTCCTTA CAGTCTGCTG 
     3661 TATCTAACAT TGCCCAGGCG CCTCTGTTTA TTCCCCCCAA TTCTGATCCT GTACCTGCCA 
     3721 GAGATTATCT TATATTGAAA GGAGTTTTAA CAGCAATGGG TTTGATAGGA ATCATTTGCC 
     3781 TTATTATAGT TGTGACACAT CATACTTTAA GCAGGAAAAA GAGAGCAGAC AAGAAAGAGA 
     3841 ATGGAACAAA ATTATTAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3901 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3961 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4021 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4081 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4141 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4201 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4261 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4321 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4381 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4441 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4501 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4561 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4621 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4681 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4741 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4801 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4861 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4921 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4981 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5041 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5101 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5161 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5221 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5281 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5341 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5401 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5461 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5521 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5581 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5641 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5701 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5761 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5821 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5881 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5941 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6001 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6061 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6121 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6181 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6241 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6301 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6361 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6421 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6481 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6541 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6601 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6661 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6721 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6781 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6841 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6901 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6961 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7021 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7081 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7141 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7201 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7261 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7321 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7381 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7441 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7501 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7561 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7621 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7681 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//