LOCUS       RC207686                8877 bp    DNA     circular  SYN 
DEFINITION  IKBKAP (Myc-DDK-tagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (IKBKAP)
ACCESSION   NM_003640
VERSION     NM_003640.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5034..5125 
                     /label="Myc-DDK"  
     misc_feature    5155..5740 
                     /label="PolyA signal"  
     misc_feature    5212..5231 
                     /label="Restriction Enzyme"  
     misc_feature    6016..6688 
                     /label="ColE1"  
     misc_feature    7118..7912 
                     /label="Kan/Neo"  
     misc_feature    7947..8249 
                     /label="SV40 ori"  
     misc_feature    8335..8812 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5016..5022 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5016 
                     /label="IKBKAP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGAAATCTG AAATTATTTC GGACCCTGGA GTTCAGGGAT ATTCAAGGTC 
     1081 CAGGGAATCC TCAGTGCTTC TCTCTCCGAA CTGAACAGGG GACGGTGCTC ATTGGTTCAG 
     1141 AACATGGCCT GATAGAAGTA GACCCTGTCT CAAGAGAAGT GAAAAATGAA GTTTCTTTGG 
     1201 TGGCAGAAGG CTTTCTCCCA GAGGATGGAA GTGGCCGCAT TGTTGGTGTT CAGGACTTGC 
     1261 TGGATCAGGA GTCTGTGTGT GTGGCCACAG CCTCTGGAGA CGTCATACTC TGCAGTCTCA 
     1321 GCACACAACA GCTGGAGTGT GTTGGGAGTG TAGCCAGTGG TATCTCTGTT ATGAGTTGGA 
     1381 GTCCTGACCA AGAGCTGGTG CTTCTTGCCA CAGGTCAACA GACCCTGATT ATGATGACAA 
     1441 AAGATTTTGA GCCAATCCTG GAGCAGCAGA TCCATCAGGA TGATTTTGGT GAAAGCAAGT 
     1501 TTATCACTGT TGGATGGGGT AGGAAGGAGA CACAGTTCCA TGGATCAGAA GGCAGACAAG 
     1561 CAGCTTTTCA GATGCAAATG CATGAGTCTG CTTTGCCCTG GGATGACCAT AGACCACAAG 
     1621 TTACCTGGCG GGGGGATGGA CAGTTTTTTG CTGTGAGTGT TGTTTGCCCA GAAACAGGGG 
     1681 CTCGGAAGGT CAGAGTGTGG AACCGAGAGT TTGCTTTGCA GTCAACCAGT GAGCCTGTGG 
     1741 CAGGACTGGG ACCAGCCCTG GCTTGGAAAC CCTCAGGCAG TTTGATTGCA TCTACACAAG 
     1801 ATAAACCCAA CCAGCAGGAT ATTGTGTTTT TTGAGAAAAA TGGACTCCTT CATGGACACT 
     1861 TTACACTTCC CTTCCTTAAA GATGAGGTTA AGGTAAATGA CTTGCTCTGG AATGCAGATT 
     1921 CCTCTGTGCT TGCAGTCTGG CTGGAAGACC TTCAGAGAGA AGAAAGCTCC ATTCCGAAAA 
     1981 CCTGTGTTCA GCTCTGGACT GTTGGAAACT ATCACTGGTA TCTCAAGCAA AGTTTATCCT 
     2041 TCAGCACCTG TGGGAAGAGC AAGATTGTGT CTCTGATGTG GGACCCTGTG ACCCCATACC 
     2101 GGCTGCATGT TCTCTGTCAG GGCTGGCATT ACCTCGCCTA TGATTGGCAC TGGACGACTG 
     2161 ACCGGAGCGT GGGAGATAAT TCAAGTGACT TGTCCAATGT GGCTGTCATT GATGGAAACA 
     2221 GGGTGTTGGT GACAGTCTTC CGGCAGACTG TGGTTCCGCC TCCCATGTGC ACCTACCAAC 
     2281 TGCTGTTCCC ACACCCTGTG AATCAAGTCA CATTCTTAGC ACACCCTCAA AAGAGTAATG 
     2341 ACCTTGCTGT TCTAGATGCC AGTAACCAGA TTTCTGTTTA TAAATGTGGT GATTGTCCAA 
     2401 GTGCTGACCC TACAGTGAAA CTGGGAGCTG TGGGTGGAAG TGGATTTAAA GTTTGCCTTA 
     2461 GAACTCCTCA TTTGGAAAAG AGATACAAAA TCCAGTTTGA GAATAATGAA GATCAAGATG 
     2521 TAAACCCGCT GAAACTAGGC CTTCTCACTT GGATTGAAGA AGACGTCTTC CTGGCTGTAA 
     2581 GCCACAGTGA GTTCAGCCCC CGGTCTGTCA TTCACCATTT GACTGCAGCT TCTTCTGAGA 
     2641 TGGATGAAGA GCATGGACAG CTCAATGTCA GTTCATCTGC AGCGGTGGAT GGGGTCATAA 
     2701 TCAGTCTATG TTGCAATTCC AAGACCAAGT CAGTAGTATT ACAGCTGGCT GATGGCCAGA 
     2761 TATTTAAGTA CCTTTGGGAG TCACCTTCTC TGGCTATTAA ACCATGGAAG AACTCTGGTG 
     2821 GATTTCCTGT TCGGTTTCCT TATCCATGCA CCCAGACCGA ATTGGCCATG ATTGGAGAAG 
     2881 AGGAATGTGT CCTTGGTCTG ACTGACAGGT GTCGCTTTTT CATCAATGAC ATTGAGGTTG 
     2941 CGTCAAATAT CACGTCATTT GCAGTATATG ATGAGTTTTT ATTGTTGACA ACCCATTCCC 
     3001 ATACCTGCCA GTGTTTTTGC CTGAGGGATG CTTCATTTAA AACATTACAG GCCGGCCTGA 
     3061 GCAGCAATCA TGTGTCCCAT GGGGAAGTTC TGCGGAAAGT GGAGAGGGGT TCACGGATTG 
     3121 TCACTGTTGT GCCCCAGGAC ACAAAGCTTG TATTACAGAT GCCAAGGGGA AACTTAGAAG 
     3181 TTGTTCATCA TCGAGCCCTG GTTTTAGCTC AGATTCGGAA GTGGTTGGAC AAACTTATGT 
     3241 TTAAAGAGGC ATTTGAATGC ATGAGAAAGC TGAGAATCAA TCTCAATCTG ATTTATGATC 
     3301 ATAACCCTAA GGTGTTTCTT GGAAATGTGG AAACCTTCAT TAAACAGATA GATTCTGTGA 
     3361 ATCATATTAA CTTGTTTTTT ACAGAATTGA AAGAAGAAGA TGTCACGAAG ACCATGTACC 
     3421 CTGCACCAGT TACCAGCAGT GTCTACCTGT CCAGGGATCC TGACGGGAAT AAAATAGACC 
     3481 TTGTCTGCGA TGCTATGAGA GCAGTCATGG AGAGCATAAA TCCTCATAAA TACTGCCTAT 
     3541 CCATACTTAC ATCTCATGTA AAGAAGACAA CCCCAGAACT GGAAATTGTA CTGCAAAAAG 
     3601 TACACGAGCT TCAAGGAAAT GCTCCCTCTG ATCCTGATGC TGTGAGTGCT GAAGAGGCCT 
     3661 TGAAATATTT GCTGCATCTG GTAGATGTTA ATGAATTATA TGATCATTCT CTTGGCACCT 
     3721 ATGACTTTGA TTTGGTCCTC ATGGTAGCTG AGAAGTCACA GAAGGATCCC AAAGAATATC 
     3781 TTCCATTTCT TAATACACTT AAGAAAATGG AAACTAATTA TCAGCGGTTT ACTATAGACA 
     3841 AATACTTGAA ACGATATGAA AAAGCCATTG GCCACCTCAG CAAATGTGGA CCTGAGTACT 
     3901 TCCCAGAATG CTTAAACTTG ATAAAAGATA AAAACTTGTA TAACGAAGCT CTGAAGTTAT 
     3961 ATTCACCAAG CTCACAACAG TACCAGGATA TCAGCATTGC TTATGGGGAG CACCTGATGC 
     4021 AGGAGCACAT GTATGAGCCA GCGGGGCTCA TGTTTGCCCG TTGCGGTGCC CACGAGAAAG 
     4081 CTCTCTCAGC CTTTCTCACA TGTGGCAACT GGAAGCAAGC CCTCTGTGTG GCAGCCCAGC 
     4141 TTAACTTTAC CAAAGACCAG CTGGTGGGCC TCGGCAGAAC TCTGGCAGGA AAGCTGGTTG 
     4201 AGCAGAGGAA GCACATTGAT GCGGCCATGG TTTTGGAAGA GTGTGCCCAG GATTATGAAG 
     4261 AAGCTGTGCT CTTGCTGTTA GAAGGAGCTG CCTGGGAAGA AGCTTTGAGG CTGGTATACA 
     4321 AATATAACAG ACTGGATATT ATAGAAACCA ACGTAAAGCC TTCCATTTTA GAAGCCCAGA 
     4381 AAAATTATAT GGCATTTCTG GACTCTCAGA CAGCCACATT CAGTCGCCAC AAGAAACGTT 
     4441 TATTGGTAGT TCGAGAGCTC AAGGAGCAAG CCCAGCAGGC AGGTCTGGAT GATGAGGTAC 
     4501 CCCACGGGCA AGAGTCAGAC CTCTTCTCTG AAACTAGCAG TGTCGTGAGT GGCAGTGAGA 
     4561 TGAGTGGCAA ATACTCCCAT AGTAACTCCA GGATATCAGC GAGATCATCC AAGAATCGCC 
     4621 GAAAAGCGGA GCGGAAGAAG CACAGCCTCA AAGAAGGCAG TCCGCTGGAG GACCTGGCCC 
     4681 TCCTGGAGGC ACTGAGTGAA GTGGTGCAGA ACACTGAAAA CCTGAAAGAT GAAGTATACC 
     4741 ATATTTTAAA GGTACTCTTT CTCTTTGAGT TTGATGAACA AGGAAGGGAA TTACAGAAGG 
     4801 CCTTTGAAGA TACGCTGCAG TTGATGGAAA GGTCACTTCC AGAAATTTGG ACTCTTACTT 
     4861 ACCAGCAGAA TTCAGCTACC CCGGTTCTAG GTCCCAATTC TACTGCAAAT AGTATCATGG 
     4921 CATCTTATCA GCAACAGAAG ACTTCGGTTC CTGTTCTTGA TGCTGAGCTT TTTATACCAC 
     4981 CAAAGATCAA CAGAAGAACC CAGTGGAAGC TGAGCCTGCT AGACACGCGT ACGCGGCCGC 
     5041 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5101 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5161 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5221 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5281 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5341 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5401 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5461 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5521 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5581 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5641 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5701 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5761 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5821 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5881 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5941 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6001 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6061 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6121 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6181 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6241 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6301 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6361 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6421 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6481 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6541 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6601 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6661 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6721 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6781 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6841 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6901 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6961 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7021 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7081 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7141 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7201 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7261 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7321 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7381 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7441 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7501 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7561 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7621 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7681 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7741 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7801 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7861 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7921 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7981 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8041 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8101 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8281 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8341 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8401 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8461 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8521 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8581 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8641 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8701 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8761 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8821 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//