LOCUS       RC207677                7275 bp    DNA     circular  SYN 
DEFINITION  PCDHB13 (Myc-DDK-tagged)-Human protocadherin beta 13 (PCDHB13)
ACCESSION   NM_018933
VERSION     NM_018933.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3432..3523 
                     /label="Myc-DDK"  
     misc_feature    3553..4138 
                     /label="PolyA signal"  
     misc_feature    3610..3629 
                     /label="Restriction Enzyme"  
     misc_feature    4414..5086 
                     /label="ColE1"  
     misc_feature    5516..6310 
                     /label="Kan/Neo"  
     misc_feature    6345..6647 
                     /label="SV40 ori"  
     misc_feature    6733..7210 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3414..3420 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3414 
                     /label="PCDHB13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCAGC GGGAAGCTCA TTTGCAGACA AAGGCAAGTC CTTTTTTCCT 
     1081 TTCTCCTTTT GGGCTTATCT CTGGCGGGCG CGGCGGAACC TAGAAGCTAT TCTGTGGTGG 
     1141 AGGAAACTGA GGGCAGCTCC TTTGTCACCA ATTTAGCAAA GGACCTGGGT CTGGAGCAGA 
     1201 GGGAATTCTC CAGGCGGGGG GTTAGGGTTG TTTCCAGAGG GAACAAACTA CATTTGCAGC 
     1261 TCAATCAGGA GACCGCGGAT TTGTTGCTAA ATGAGAAATT GGACCGTGAG GATCTGTGCG 
     1321 GTCACACAGA GCCCTGTGTG CTACGTTTCC AAGTGTTGCT AGAGAGTCCC TTCGAGTTTT 
     1381 TTCAAGCTGA GCTGCAAGTA ATAGACATAA ACGACCACTC TCCAGTATTT CTGGACAAAC 
     1441 AAATGTTGGT GAAAGTATCA GAGAGCAGTC CTCCTGGGAC TACGTTTCCT CTGAAGAATG 
     1501 CCGAAGACTT AGATGTAGGC CAAAACAATA TTGAGAACTA TATAATCAGC CCCAACTCCT 
     1561 ATTTTCGGGT CCTCACCCGC AAACGCAGTG ATGGCAGGAA ATACCCAGAG CTGGTGCTGG 
     1621 ACAAAGCGCT GGACCGAGAG GAAGAAGCTG AGCTCAGGTT AACACTCACA GCACTGGATG 
     1681 GTGGCTCTCC GCCCAGATCT GGCACTGCTC AGGTCTACAT CGAAGTCCTG GATGTCAACG 
     1741 ATAATGCCCC TGAATTTGAG CAGCCTTTCT ATAGAGTGCA GATCTCTGAG GACAGTCCGG 
     1801 TAGGCTTCCT GGTTGTGAAG GTCTCTGCCA CGGATGTAGA CACAGGAGTC AACGGAGAGA 
     1861 TTTCCTATTC ACTTTTCCAA GCTTCAGAAG AGATTGGCAA AACCTTTAAG ATCAATCCCT 
     1921 TGACAGGAGA AATTGAACTA AAAAAACAAC TCGATTTCGA AAAACTTCAG TCCTATGAAG 
     1981 TCAATATTGA GGCAAGAGAT GCTGGAACCT TTTCTGGAAA ATGCACCGTT CTGATTCAAG 
     2041 TGATAGATGT GAACGACCAT GCCCCAGAAG TTACCATGTC TGCATTTACC AGCCCAATAC 
     2101 CTGAGAACGC GCCTGAAACT GTGGTTGCAC TTTTCAGTGT TTCAGATCTT GATTCAGGAG 
     2161 AAAATGGGAA AATTAGTTGC TCCATTCAGG AGGATCTACC CTTCCTCCTG AAATCCGCGG 
     2221 AAAACTTTTA CACCCTACTA ACGGAGAGAC CACTAGACAG AGAAAGCAGA GCGGAATACA 
     2281 ACATCACTAT CACTGTCACT GACTTGGGGA CCCCTATGCT GATAACACAG CTCAATATGA 
     2341 CCGTGCTGAT CGCCGATGTC AATGACAACG CTCCCGCCTT CACCCAAACC TCCTACACCC 
     2401 TGTTCGTCCG CGAGAACAAC AGCCCCGCCC TGCACATCCG CAGCGTCAGC GCTACAGACA 
     2461 GAGACTCAGG CACCAACGCC CAGGTCACCT ACTCGCTGCT GCCGCCCCAG GACCCGCACC 
     2521 TGCCCCTCAC ATCCCTGGTC TCCATCAACG CGGACAACGG CCACCTGTTC GCCCTCAGGT 
     2581 CTCTGGACTA CGAGGCCCTG CAGGGGTTCC AGTTCCGCGT GGGCGCTTCA GACCACGGCT 
     2641 CCCCGGCGCT GAGCAGCGAG GCGCTGGTGC GCGTGGTGGT GCTGGACGCC AACGACAACT 
     2701 CGCCCTTCGT GCTGTACCCG CTGCAGAACG GCTCCGCGCC CTGCACCGAG CTGGTGCCCC 
     2761 GGGCGGCCGA GCCGGGCTAC CTGGTGACCA AGGTGGTGGC GGTGGACGGC GACTCGGGCC 
     2821 AGAACGCCTG GCTGTCGTAC CAGCTGCTCA AGGCCACGGA GCTCGGTCTG TTCGGCGTGT 
     2881 GGGCGCACAA TGGCGAGGTG CGCACCGCCA GGCTGCTGAG CGAGCGCGAC GCGGCCAAGC 
     2941 ACAGGCTGGT GGTGCTGGTC AAGGACAATG GCGAGCCTCC GCGCTCGGCC ACCGCCACGC 
     3001 TGCACGTGCT CCTGGTGGAC GGCTTCTCCC AGCCCTACCT GCCTCTCCCG GAGGCGGCCC 
     3061 CGACCCAGGC CCAGGCCGAC TTGCTCACCG TCTACCTGGT GGTGGCGTTG GCCTCGGTGT 
     3121 CTTCGCTCTT CCTCTTTTCG GTGCTCCTGT TCGTGGCGGT GCGGCTGTGT AGGAGGAGCA 
     3181 GGGCGGCCTC GGTGGGTCGC TGCTTGGTGC CCGAGGGCCC CCTTCCAGGG CATCTTGTGG 
     3241 ACATGAGCGG CACCAGGACC CTATCCCAGA GCTACCAGTA TGAGGTGTGT CTGGCAGGAG 
     3301 GCTCAGGGAC CAATGAGTTC AAGTTCCTGA AGCCGATTAT CCCCAACTTC CCTCCCCAGT 
     3361 GCCCTGGGAA AGAAATACAA GGAAATTCTA CCTTCCCCAA TAACTTTGGG TTCAATATTC 
     3421 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3481 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3541 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3601 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3661 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3721 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3781 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3841 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3901 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3961 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4021 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4081 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4141 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4201 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4261 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4321 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4381 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4441 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4501 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4561 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4621 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4681 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4741 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4801 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4861 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4921 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4981 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5041 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5101 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5161 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5221 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5281 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5341 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5401 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5461 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5521 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5581 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5641 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5701 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5761 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5821 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5881 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5941 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6001 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6061 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6121 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6181 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6241 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6301 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6361 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6421 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6481 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6541 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6601 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6661 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6721 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6781 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6841 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6901 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6961 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7021 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7081 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7141 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7201 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7261 TTTAACGCGA ATTTT
//