LOCUS       RC207668                8133 bp    DNA     circular  SYN 
DEFINITION  FUK (Myc-DDK-tagged)-Human fucokinase (FUK)
ACCESSION   NM_145059
VERSION     NM_145059.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4290..4381 
                     /label="Myc-DDK"  
     misc_feature    4411..4996 
                     /label="PolyA signal"  
     misc_feature    4468..4487 
                     /label="Restriction Enzyme"  
     misc_feature    5272..5944 
                     /label="ColE1"  
     misc_feature    6374..7168 
                     /label="Kan/Neo"  
     misc_feature    7203..7505 
                     /label="SV40 ori"  
     misc_feature    7591..8068 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4272..4278 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4272 
                     /label="FUK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCAGCCG AAGGGAGTTG ATTGGACAGT CATCATCCTG ACCTGCCAGT 
     1081 ACAAGGACAG TGTCCAGGTC TTTCAGAGAG AACTGGAAGT GCGGCAGAAG CGGGAGCAGA 
     1141 TCCCTGCTGG GACGCTGTTA CTGGCCGTGG AGGACCCAGA GAAGCGTGTG GGCAGCGGAG 
     1201 GAGCCACCCT CAACGCCCTG CTGGTGGCTG CTGAACACCT GAGTGCCCGG GCAGGCTTCA 
     1261 CTGTGGTCAC ATCCGATGTC CTGCACTCGG CCTGGATCCT CATTCTGCAC ATGGGTCGAG 
     1321 ACTTCCCCTT TGATGACTGT GGCAGGGCTT TCACCTGCCT CCCCGTGGAG AACCCCGAGG 
     1381 CCCCCGTGGA AGCCTTGGTC TGCAACCTGG ACTGCCTGCT GGACATCATG ACCTATCGGC 
     1441 TGGGCCCGGG CTCCCCGCCA GGCGTGTGGG TCTGCAGCAC CGACATGCTG CTGTCTGTTC 
     1501 CTGCAAATCC TGGTATCAGC TGGGACAGCT TCCGGGGAGC CAGAGTGATC GCCCTCCCAG 
     1561 GGAGCCCGGC CTACGCTCAG AATCATGGCG TCTACCTAAC TGACCCCCAG GGCCTTGTTT 
     1621 TGGACATTTA CTACCAGGGC ACTGAGGCAG AGATTCAGCG GTGTGTCAGG CCTGATGGGC 
     1681 GGGTGCCACT GGTCTCTGGG GTTGTCTTCT TCTCTGTGGA GACTGCCGAG CGCCTCCTAG 
     1741 CCACCCACGT GAGCCCGCCC CTGGATGCCT GCACCTACCT AGGCTTGGAC TCCGGAGCCC 
     1801 GGCCTGTCCA GCTGTCTCTG TTTTTTGACA TTCTCCACTG CATGGCTGAG AACGTGACCA 
     1861 GGGAGGACTT CCTGGTGGGG AGGCCCCCAG AGTTGGGGCA AGGCGATGCA GATGTAGCGG 
     1921 GTTATCTGCA GAGCGCCCGG GCCCAGCTGT GGAGGGAGCT TCGCGATCAG CCCCTTACCA 
     1981 TGGCCTATGT CTCCAGCGGC AGCTACAGCT ACATGACCTC CTCAGCCAGT GAGTTCCTGC 
     2041 TCAGCCTCAC ACTCCCCGGG GCTCCTGGGG CCCAGATTGT GCACTCCCAG GTGGAGGAGC 
     2101 AGCAGCTTCT GGCGGCCGGG AGCTCTGTGG TCAGCTGCCT GCTGGAGGGC CCTGTCCAGC 
     2161 TGGGTCCTGG GAGCGTCCTG CAGCACTGCC ACCTGCAGGG CCCCATTCAC ATAGGCGCTG 
     2221 GCTGCTTGGT GACTGGCCTG GATACAGCCC ACTCCAAGGC CCTGCATGGC CGGGAGCTGC 
     2281 GTGACCTTGT CCTGCAGGGA CACCACACGC GGCTACACGG CTCCCCGGGC CACGCCTTCA 
     2341 CCCTCGTTGG CCGTCTGGAC AGCTGGGAGA GACAGGGGGC AGGCACATAT CTCAACGTGC 
     2401 CCTGGAGTGA ATTCTTCAAG AGGACAGGTG TTCGAGCCTG GGACCTGTGG GACCCTGAGA 
     2461 CGCTGCCCGC AGAGTACTGC CTTCCCAGCG CCCGCCTCTT TCCTGTGCTC CACCCCTCGA 
     2521 GGGAGCTGGG ACCCCAGGAC CTGCTGTGGA TGCTGGACCA CCAGGAGGAT GGGGGCGAGG 
     2581 CCCTGCGAGC CTGGCGGGCC TCCTGGCGCC TGTCCTGGGA GCAGCTGCAG CCGTGCCTGG 
     2641 ATCGGGCTGC CACGCTGGCC TCTCGCCGGG ACCTGTTCTT CCGCCAGGCC CTGCATAAGG 
     2701 CGCGGCACGT GCTGGAGGCC CGGCAGGACC TCAGCCTGCG CCCGCTGATC TGGGCTGCTG 
     2761 TCCGCGAGGG CTGCCCCGGG CCCCTGCTGG CCACGCTGGA CCAGGTTGCA GCTGGGGCAG 
     2821 GAGACCCTGG TGTGGCGGCA CGGGCACTGG CCTGTGTGGC GGACGTCCTG GGCTGCATGG 
     2881 CAGAGGGCCG TGGGGGCTTG CGGAGCGGGC CAGCTGCCAA CCCTGAGTGG ATGCGGCCCT 
     2941 TCTCATACCT GGAGTGTGGA GACCTGGCAG CGGGCGTGGA GGCGCTTGCC CAGGAGAGGG 
     3001 ACAAGTGGCT AAGCAGGCCA GCCTTGCTGG TGCGAGCGGC CCGCCACTAT GAGGGGGCTG 
     3061 GTCAGATCCT GATCCGCCAG GCTGTGATGT CAGCCCAGCA CTTTGTCTCC ACAGAGCAGG 
     3121 TGGAACTGCC GGGACCTGGG CAGTGGGTGG TGGCTGAGTG CCCGGCCCGT GTGGATTTCT 
     3181 CTGGGGGCTG GAGTGACACG CCACCCCTTG CCTATGAGCT TGGCGGGGCT GTGCTGGGCC 
     3241 TGGCTGTGCG AGTGGACGGC CGCCGGCCCA TCGGAGCCAG GGCACGCCGC ATCCCGGAGC 
     3301 CTGAGCTGTG GCTGGCGGTG GGGCCTCGGC AGGATGAGAT GACTGTGAAG ATAGTGTGCC 
     3361 GGTGCCTGGC TGACCTGCGG GACTACTGCC AGCCTCATGC CCCAGGGGCC CTGCTGAAGG 
     3421 CGGCCTTCAT CTGTGCAGGG ATCGTGCATG TCCACTCGGA ACTCCAGCTG AGTGAGCAGC 
     3481 TGCTCCGCAC CTTCGGGGGC GGCTTTGAGC TGCACACCTG GTCTGAGCTG CCCCACGGCT 
     3541 CTGGCCTGGG CACCAGCAGC ATCCTGGCAG GCACTGCCCT GGCTGCCTTG CAGCGAGCCG 
     3601 CAGGCCGGGT GGTGGGCACG GAAGCCCTGA TCCACGCAGT GCTGCACCTG GAGCAGGTGC 
     3661 TCACCACTGG AGGTGGCTGG CAGGACCAAG TAGGTGGCCT AATGCCTGGC ATCAAGGTGG 
     3721 GGCGCTCCCG GGCTCAGCTG CCACTGAAGG TGGAGGTAGA AGAGGTCACG GTGCCTGAGG 
     3781 GCTTTGTCCA GAAGCTCAAT GACCACCTGC TCTTGGTGTA CACTGGCAAG ACCCGCCTGG 
     3841 CTCGGAACCT GCTGCAGGAT GTGCTGAGGA GCTGGTATGC CCGACTTCCT GCTGTGGTGC 
     3901 AGAATGCCCA CAGCCTGGTA CGGCAAACTG AGGAGTGTGC TGAAGGCTTC CGCCAAGGAA 
     3961 GCCTGCCTCT GCTGGGCCAG TGCCTGACCT CGTACTGGGA GCAGAAGAAG CTCATGGCTC 
     4021 CAGGCTGTGA GCCCCTGACT GTGCGGCGTA TGATGGATGT CCTGGCCCCC CACGTGCATG 
     4081 GCCAGAGCCT GGCTGGGGCA GGCGGTGGAG GCTTTCTCTA TCTGTTGACC AAGGAGCCAC 
     4141 AGCAAAAGGA GGCCTTGGAG GCGGTGCTGG CCAAGACCGA GGGCCTTGGG AATTACAGCA 
     4201 TCCACCTGGT TGAAGTGGAC ACTCAGGGCC TGAGCCTGAA GCTGCTGGGG ACCGAGGCCT 
     4261 CAACCTGTTG CCCTTTCCCA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4321 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4381 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4441 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4501 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4561 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4621 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4681 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4741 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4801 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4861 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4921 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4981 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5041 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5101 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5161 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5221 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5281 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5341 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5401 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5461 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5521 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5581 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5641 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5701 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5761 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5821 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5881 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5941 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6001 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6061 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6121 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6181 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6241 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6301 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6361 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6421 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6481 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6541 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6601 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6661 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6721 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6781 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6841 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6901 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6961 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7021 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7081 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7141 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7201 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7261 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7321 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7381 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7441 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7561 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7621 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7681 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7741 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7801 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7861 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7921 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7981 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8041 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8101 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//