LOCUS       RC207614L1                9261 bp    DNA     circular  SYN 
DEFINITION  USO1 (Myc-DDK-tagged)-Human USO1 vesicle docking protein homolog (yeast) (USO1)
ACCESSION   NM_003715
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7552..7732 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8460..9119 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7891..8459 
                     /label="ColE1"  
     misc_feature    6427..6447 
                     /label="LR50 primer"  
     misc_feature    6224..6295 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6436..7027 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6206..6212 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6206 
                     /label="USO1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAATTTCCTC CGCGGGGTAA TGGGGGGTCA 
     3361 GAGTGCCGGA CCCCAGCACA CAGAAGCCGA GACGATTCAA AAGCTTTGTG ACAGAGTAGC 
     3421 TTCATCTACT TTATTGGATG ATCGAAGAAA TGCTGTTCGT GCTCTCAAAT CATTATCTAA 
     3481 GAAATACCGC TTGGAAGTGG GTATACAAGC TATGGAACAT CTTATTCATG TTTTACAAAC 
     3541 AGATCGTTCA GATTCTGAAA TAATAGGTTA TGCTTTGGAC ATACTATATA ATATAATATC 
     3601 TAATGAAGAA GAAGAAGAAG TAGAAGAAAA TTCCACAAGA CAGAGTGAAG ATTTGGGAAG 
     3661 CCAATTTACA GAAATTTTCA TTAAGCAGCA GGAAAATGTC ACTCTTCTGT TATCTTTATT 
     3721 GGAGGAGTTT GATTTCCATG TCCGCTGGCC TGGTGTGAAG CTTCTTACTT CTCTTTTAAA 
     3781 ACAACTAGGG CCTCAGGTGC AACAAATTAT TTTAGTCAGT CCTATGGGTG TTTCAAGATT 
     3841 GATGGACTTA CTAGCGGATT CCAGGGAAGT TATACGTAAT GATGGCGTCT TACTACTGCA 
     3901 GGCACTAACA AGAAGCAATG GTGCAATCCA GAAAATTGTT GCTTTTGAAA ATGCTTTCGA 
     3961 GAGACTACTG GACATTATTT CAGAGGAGGG GAACAGTGAT GGAGGTATAG TAGTTGAAGA 
     4021 TTGTTTGATT TTGCTCCAAA ACTTATTAAA AAACAACAAC TCCAATCAAA ATTTTTTTAA 
     4081 AGAAGGCTCA TATATTCAAC GTATGAAACC TTGGTTTGAA GTTGGAGATG AAAATTCTGG 
     4141 CTGGTCTGCA CAGAAAGTGA CCAATCTACA TCTAATGCTA CAGCTTGTTC GTGTATTGGT 
     4201 ATCTCCCACC AACCCTCCTG GTGCTACCAG TAGCTGCCAG AAGGCTATGT TCCAGTGTGG 
     4261 GTTATTGCAG CAGCTTTGTA CTATCCTAAT GGCTACTGGG GTTCCTGCTG ATATCCTGAC 
     4321 TGAGACCATA AATACTGTAT CAGAAGTTAT TCGAGGCTGC CAAGTAAACC AAGACTACTT 
     4381 TGCATCTGTA AATGCACCTT CAAACCCACC AAGACCGGCA ATTGTAGTAC TTCTCATGTC 
     4441 CATGGTTAAT GAAAGGCAGC CATTTGTTTT GCGCTGTGCT GTTCTCTATT GTTTCCAGTG 
     4501 TTTCTTGTAT AAAAACCAAA AAGGACAAGG AGAAATCGTG TCAACACTTT TACCTTCTAC 
     4561 CATTGATGCA ACAGGTAATT CAGTTTCAGC TGGCCAGTTA TTATGTGGAG GTTTGTTTTC 
     4621 TACTGATTCA CTTTCAAACT GGTGTGCTGC TGTGGCCCTT GCCCATGCGT TGCAAGAAAA 
     4681 TGCCACCCAG AAAGAACAGT TGCTCAGGGT TCAACTTGCT ACAAGTATTG GCAACCCTCC 
     4741 AGTTTCTTTA CTTCAACAGT GCACCAATAT TCTTTCACAG GGAAGCAAAA TACAAACAAG 
     4801 AGTTGGATTA TTAATGTTGC TTTGTACCTG GCTAAGCAAT TGTCCCATTG CAGTAACGCA 
     4861 TTTTCTTCAC AATTCAGCCA ATGTTCCATT TCTTACAGGA CAAATTGCAG AAAATCTTGG 
     4921 AGAAGAAGAG CAGTTGGTCC AAGGCTTATG TGCCCTTTTG TTGGGCATTT CGATTTATTT 
     4981 CAATGATAAC TCACTTGAGA GCTACATGAA AGAGAAGCTA AAACAACTGA TTGAGAAGAG 
     5041 GATTGGCAAA GAGAATTTCA TAGAGAAACT AGGATTTATT AGCAAACATG AGTTGTATTC 
     5101 CAGAGCATCT CAGAAACCCC AGCCAAACTT TCCCAGTCCA GAATACATGA TATTTGATCA 
     5161 TGAGTTTACG AAGCTGGTAA AAGAACTTGA AGGTGTTATA ACTAAGGCTA TTTATAAGTC 
     5221 CAGTGAAGAA GATAAAAAAG AAGAAGAGGT GAAAAAAACA TTAGAACAGC ATGACAATAT 
     5281 TGTGACTCAC TACAAAAATA TGATTCGAGA GCAGGATCTC CAACTTGAGG AATTAAGGCA 
     5341 GCAGGTTTCT ACATTAAAAT GTCAAAATGA ACAGCTCCAG ACGGCAGTCA CACAGCAAGT 
     5401 ATCACAGATC CAGCAGCACA AAGACCAGTA TAATCTTCTT AAAATACAGC TAGGAAAAGA 
     5461 CAATCAGCAT CAAGGTTCTT ACAGTGAGGG GGCTCAGATG AATGGCATTC AGCCAGAAGA 
     5521 AATTGGTAGA TTGCGAGAAG AGATAGAAGA ATTAAAACGT AATCAGGAAC TTTTACAAAG 
     5581 CCAGCTGACT GAAAAGGACT CTATGATTGA AAATATGAAA TCTTCCCAAA CATCTGGCAC 
     5641 AAATGAACAG TCTTCAGCAA TAGTTTCAGC TAGAGATTCT GAACAAGTTG CAGAATTAAA 
     5701 ACAGGAACTG GCAACTTTAA AGTCTCAGTT AAACTCACAA TCTGTGGAGA TCACCAAACT 
     5761 ACAGACAGAA AAGCAGGAAC TGTTACAGAA AACAGAAGCG TTTGCAAAAT CAGTTGAGGT 
     5821 ACAAGGAGAG ACCGAGACTA TAATAGCCAC CAAAACTACT GATGTAGAAG GAAGACTGTC 
     5881 AGCATTATTA CAAGAGACCA AAGAGTTAAA GAATGAAATT AAAGCTCTGT CTGAGGAAAG 
     5941 AACTGCCATT AAAGAGCAGC TGGATTCATC TAATAGTACC ATTGCCATTT TACAAACTGA 
     6001 GAAAGACAAA CTAGAGTTGG AAATTACAGA TTCTAAAAAA GAACAAGATG ATCTCTTGGT 
     6061 GCTCTTGGCC GATCAAGATC AGAAAATACT GTCATTGAAG AATAAACTCA AGGATCTTGG 
     6121 TCATCCAGTT GAAGAAGAGG ATGAACTTGA ATCTGGAGAC CAAGAGGATG AGGATGATGA 
     6181 AAGTGAAGAT CCTGGCAAGG ATCTAGATCA TATCACGCGT ACGCGGCCGC TCGAGCAGAA 
     6241 ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA 
     6301 TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA CAAGTAGGAT TCGTCGAGGG ACCTAATAAC 
     6361 TTCGTATAGC ATACATTATA CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA 
     6421 CAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 
     6481 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 
     6541 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 
     6601 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 
     6661 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 
     6721 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 
     6781 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA 
     6841 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 
     6901 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 
     6961 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 
     7021 GCCGCCTCCC CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT 
     7081 CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA 
     7141 GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC 
     7201 AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC 
     7261 CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA 
     7321 TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA 
     7381 CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG 
     7441 CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG 
     7501 GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC 
     7561 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC 
     7621 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 
     7681 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 
     7741 CAGCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     7801 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     7861 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     7921 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     7981 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     8041 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     8101 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     8161 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     8221 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     8281 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     8341 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     8401 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     8461 GGTCATGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT 
     8521 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC 
     8581 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC 
     8641 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA 
     8701 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA 
     8761 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT 
     8821 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC 
     8881 ACCAGCTCAC CGTCTTTCAT TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA 
     8941 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG 
     9001 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC 
     9061 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT 
     9121 TTCTCCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     9181 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTCCC GCGCACATTT 
     9241 CCCCGAAAAG TGCCACCTGA C
//