LOCUS       RC207612                8748 bp    DNA     circular  SYN 
DEFINITION  PEX1 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 1 (PEX1)
ACCESSION   NM_000466
VERSION     NM_000466.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4903..4994 
                     /label="Myc-DDK"  
     misc_feature    5024..5609 
                     /label="PolyA signal"  
     misc_feature    5081..5100 
                     /label="Restriction Enzyme"  
     misc_feature    5885..6557 
                     /label="ColE1"  
     misc_feature    6987..7781 
                     /label="Kan/Neo"  
     misc_feature    7816..8118 
                     /label="SV40 ori"  
     misc_feature    8204..8681 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4878..4885 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4878 
                     /label="PEX1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG TGGGGCAGCG ATCGCCTGGC GGGTGCTGGG GGAGGCGGGG 
     1081 CGGCAGTGAC TGTGGCCTTC ACCAACGCTC GCGACTGCTT CCTCCACCTG CCGCGGCGTC 
     1141 TCGTGGCCCA GCTGCATCTG CTGCAGAATC AAGCTATAGA AGTGGTCTGG AGTCACCAGC 
     1201 CTGCATTCTT GAGCTGGGTG GAAGGCAGGC ATTTTAGTGA TCAAGGTGAA AATGTGGCTG 
     1261 AAATTAACAG ACAAGTTGGT CAAAAACTTG GACTCTCAAA TGGGGGACAG GTATTTCTCA 
     1321 AGCCATGTTC CCATGTGGTA TCTTGTCAAC AAGTTGAGGT GGAACCCCTC TCAGCAGATG 
     1381 ATTGGGAGAT ACTGGAGCTG CATGCTGTTT CCCTTGAACA ACATCTTCTA GATCAAATTC 
     1441 GAATAGTTTT TCCAAAAGCC ATTTTTCCTG TTTGGGTTGA TCAACAAACG TACATATTTA 
     1501 TCCAAATTGT TGCACTAATA CCAGCTGCCT CTTATGGAAG GCTGGAAACT GACACCAAAC 
     1561 TCCTTATTCA GCCAAAGACA CGCCGAGCCA AAGAGAATAC ATTTTCAAAA GCTGATGCTG 
     1621 AATATAAAAA ACTTCATAGT TATGGAAGAG ACCAGAAAGG AATGATGAAA GAACTTCAAA 
     1681 CCAAGCAACT TCAGTCAAAT ACTGTGGGAA TCACTGAATC TAATGAAAAC GAGTCAGAGA 
     1741 TTCCAGTTGA CTCATCATCA GTAGCAAGTT TATGGACTAT GATAGGAAGC ATTTTTTCCT 
     1801 TTCAATCTGA GAAGAAACAA GAGACATCTT GGGGTTTAAC TGAAATCAAT GCATTCAAAA 
     1861 ATATGCAGTC AAAGGTTGTT CCTCTAGACA ATATTTTCAG AGTATGCAAA TCTCAACCTC 
     1921 CTAGTATATA TAACGCGTCA GCAACCTCTG TTTTTCATAA ACACTGTGCC ATTCATGTAT 
     1981 TTCCATGGGA CCAGGAATAT TTTGATGTAG AGCCCAGCTT TACTGTGACA TATGGAAAGC 
     2041 TAGTTAAGCT ACTTTCTCCA AAGCAACAGC AAAGTAAAAC AAAACAAAAT GTGTTATCAC 
     2101 CTGAAAAAGA GAAGCAGATG TCAGAGCCAC TAGATCAAAA AAAAATTAGG TCAGATCATA 
     2161 ATGAAGAAGA TGAGAAGGCC TGTGTGCTAC AAGTAGTCTG GAATGGACTT GAAGAATTGA 
     2221 ACAATGCCAT CAAATATACC AAAAATGTAG AAGTTCTCCA TCTTGGGAAA GTCTGGATTC 
     2281 CAGATGACCT GAGGAAGAGA CTAAATATAG AAATGCATGC CGTAGTCAGG ATAACTCCAG 
     2341 TGGAAGTTAC CCCTAAAATT CCAAGATCTC TAAAGTTACA ACCTAGAGAG AATTTACCTA 
     2401 AAGACATAAG TGAAGAAGAC ATAAAAACTG TATTTTATTC ATGGCTACAG CAGTCTACTA 
     2461 CCACCATGCT TCCTTTGGTA ATATCAGAGG AAGAATTTAT TAAGCTGGAA ACTAAAGATG 
     2521 GACTGAAGGA ATTTTCTCTG AGTATAGTTC ATTCTTGGGA AAAAGAAAAA GATAAAAATA 
     2581 TTTTTCTGTT GAGTCCCAAT TTGCTGCAGA AGACTACAAT ACAAGTCCTT CTAGATCCTA 
     2641 TGGTAAAAGA AGAAAACAGT GAGGAAATTG ACTTTATTCT TCCTTTTTTA AAGCTGAGCT 
     2701 CTTTGGGAGG AGTGAATTCC TTAGGCGTAT CCTCCTTGGA GCACATCACT CACAGCCTCC 
     2761 TGGGACGCCC TTTGTCTCGG CAGCTGATGT CTCTTGTTGC AGGACTTAGG AATGGAGCTC 
     2821 TTTTACTCAC AGGAGGAAAG GGAAGTGGAA AATCAACTTT AGCCAAAGCA ATCTGTAAAG 
     2881 AAGCATTTGA CAAACTGGAT GCCCATGTGG AGAGAGTTGA CTGTAAAGCT TTACGAGGAA 
     2941 AAAGGCTTGA AAACATACAA AAAACCCTAG AGGTGGCTTT CTCAGAGGCA GTGTGGATGC 
     3001 AGCCATCTGT TGTCCTGCTG GATGACCTTG ACCTCATTGC TGGACTGCCT GCTGTCCCGG 
     3061 AACATGAGCA CAGTCCTGAT GCGGTGCAGA GCCAGCGGCT TGCTCATGCT TTGAATGATA 
     3121 TGATAAAAGA GTTTATCTCC ATGGGAAGTT TGGTTGCACT GATTGCCACA AGTCAGTCTC 
     3181 AGCAATCTCT ACATCCTTTA CTTGTTTCTG CTCAAGGAGT TCACATATTT CAGTGCGTCC 
     3241 AACACATTCA GCCTCCTAAT CAGGAACAAA GATGTGAAAT TCTGTGTAAT GTAATAAAAA 
     3301 ATAAATTGGA CTGTGATATA AACAAGTTCA CCGATCTTGA CCTGCAGCAT GTAGCTAAAG 
     3361 AAACTGGAGG GTTTGTGGCT AGAGATTTTA CAGTACTTGT GGATCGAGCC ATACATTCTC 
     3421 GACTCTCTCG TCAGAGTATA TCCACCAGAG AAAAATTAGT TTTAACAACA TTGGACTTCC 
     3481 AAAAGGCTCT CCGCGGATTT CTTCCTGCGT CTTTGCGAAG TGTCAACCTG CATAAACCTA 
     3541 GAGACCTGGG TTGGGACAAG ATTGGTGGGT TACATGAAGT TAGGCAGATA CTCATGGATA 
     3601 CTATCCAGTT ACCTGCCAAG TATCCAGAAT TATTTGCAAA CTTGCCCATA CGACAAAGAA 
     3661 CAGGAATACT GTTGTATGGT CCGCCTGGAA CAGGAAAAAC CTTACTAGCT GGGGTAATTG 
     3721 CACGAGAGAG TAGAATGAAT TTTATAAGTG TCAAGGGGCC AGAGTTACTC AGCAAATACA 
     3781 TTGGAGCAAG TGAACAAGCT GTTCGGGATA TTTTTATTAG AGCACAGGCT GCAAAGCCCT 
     3841 GCATTCTTTT CTTTGATGAA TTTGAATCCA TTGCTCCTCG GCGGGGTCAT GATAATACAG 
     3901 GAGTTACAGA CCGAGTAGTT AACCAGTTGC TGACTCAGTT GGATGGAGTA GAAGGCTTAC 
     3961 AGGGTGTTTA TGTATTGGCT GCTACTAGTC GCCCTGACTT GATTGACCCT GCCCTGCTTA 
     4021 GGCCTGGTCG ACTAGATAAA TGTGTATACT GTCCTCCTCC TGATCAGGTG TCACGTCTTG 
     4081 AAATTTTAAA TGTCCTCAGT GACTCTCTAC CTCTGGCAGA TGATGTTGAC CTTCAGCATG 
     4141 TAGCATCAGT AACTGACTCC TTTACTGGAG CTGATCTGAA AGCTTTACTT TACAATGCCC 
     4201 AATTGGAGGC CTTACATGGA ATGCTGCTCT CGAGTGGACT CCAGGATGGA AGTTCCAGCT 
     4261 CTGATAGTGA CCTAAGTCTG TCTTCAATGG TCTTTCTTAA CCATAGCAGT GGCTCTGACG 
     4321 ATTCAGCTGG AGATGGAGAA TGTGGCTTAG ATCAGTCCCT TGTTTCTTTA GAGATGTCCG 
     4381 AGATCCTTCC AGATGAATCA AAATTCAATA TGTACCGGCT CTACTTTGGA AGCTCTTATG 
     4441 AATCAGAACT TGGAAATGGA ACCTCTTCTG ATTTGAGCTC ACAATGTCTC TCTGCACCAA 
     4501 GCTCCATGAC TCAGGATTTG CCTGGAGTTC CTGGGAAAGA CCAGTTGTTT TCACAGCCTC 
     4561 CAGTGTTAAG GACAGCTTCA CAAGAGGGTT GCCAAGAACT TACACAAGAA CAAAGAGATC 
     4621 AACTGAGGGC AGATATCAGT ATTATCAAAG GCAGATACCG GAGCCAAAGT GGAGAGGACG 
     4681 AATCCATGAA CCAACCAGGA CCAATCAAAA CCAGACTGGC TATTAGTCAG TCACATTTAA 
     4741 TGACTGCACT TGGTCACACA AGACCATCCA TTAGTGAAGA TGACTGGAAG AATTTTGCTG 
     4801 AGCTATATGA AAGCTTTCAA AATCCAAAGA GGAGAAAAAA TCAAAGTGGA ACAATGTTTC 
     4861 GACCTGGACA GAAAGTAACT TTAGCAAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     4921 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4981 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5041 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5101 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5161 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5221 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5281 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5341 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5401 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5461 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5521 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5581 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5641 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5701 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5761 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5821 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5881 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5941 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6001 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6061 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6121 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6181 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6241 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6301 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6361 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6421 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6481 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6541 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6601 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6661 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6721 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6781 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6841 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6901 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6961 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7021 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7081 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7141 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7201 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7261 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7321 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7381 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7441 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7501 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7561 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7621 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7681 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7741 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7801 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7861 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7921 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7981 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8101 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8161 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8221 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8281 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8341 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8401 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8461 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8521 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8581 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8641 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8701 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//