LOCUS       RC207597                7233 bp    DNA     circular  SYN 
DEFINITION  TLR2 (Myc-DDK-tagged)-Human toll-like receptor 2 (TLR2)
ACCESSION   NM_003264
VERSION     NM_003264.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3390..3481 
                     /label="Myc-DDK"  
     misc_feature    3511..4096 
                     /label="PolyA signal"  
     misc_feature    3568..3587 
                     /label="Restriction Enzyme"  
     misc_feature    4372..5044 
                     /label="ColE1"  
     misc_feature    5474..6268 
                     /label="Kan/Neo"  
     misc_feature    6303..6605 
                     /label="SV40 ori"  
     misc_feature    6691..7168 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3372..3378 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3372 
                     /label="TLR2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACATACT TTGTGGATGG TGTGGGTCTT GGGGGTCATC ATCAGCCTCT 
     1081 CCAAGGAAGA ATCCTCCAAT CAGGCTTCTC TGTCTTGTGA CCGCAATGGT ATCTGCAAGG 
     1141 GCAGCTCAGG ATCTTTAAAC TCCATTCCCT CAGGGCTCAC AGAAGCTGTA AAAAGCCTTG 
     1201 ACCTGTCCAA CAACAGGATC ACCTACATTA GCAACAGTGA CCTACAGAGG TGTGTGAACC 
     1261 TCCAGGCTCT GGTGCTGACA TCCAATGGAA TTAACACAAT AGAGGAAGAT TCTTTTTCTT 
     1321 CCCTGGGCAG TCTTGAACAT TTAGACTTAT CCTATAATTA CTTATCTAAT TTATCGTCTT 
     1381 CCTGGTTCAA GCCCCTTTCT TCTTTAACAT TCTTAAACTT ACTGGGAAAT CCTTACAAAA 
     1441 CCCTAGGGGA AACATCTCTT TTTTCTCATC TCACAAAATT GCAAATCCTG AGAGTGGGAA 
     1501 ATATGGACAC CTTCACTAAG ATTCAAAGAA AAGATTTTGC TGGACTTACC TTCCTTGAGG 
     1561 AACTTGAGAT TGATGCTTCA GATCTACAGA GCTATGAGCC AAAAAGTTTG AAGTCAATTC 
     1621 AGAACGTAAG TCATCTGATC CTTCATATGA AGCAGCATAT TTTACTGCTG GAGATTTTTG 
     1681 TAGATGTTAC AAGTTCCGTG GAATGTTTGG AACTGCGAGA TACTGATTTG GACACTTTCC 
     1741 ATTTTTCAGA ACTATCCACT GGTGAAACAA ATTCATTGAT TAAAAAGTTT ACATTTAGAA 
     1801 ATGTGAAAAT CACCGATGAA AGTTTGTTTC AGGTTATGAA ACTTTTGAAT CAGATTTCTG 
     1861 GATTGTTAGA ATTAGAGTTT GATGACTGTA CCCTTAATGG AGTTGGTAAT TTTAGAGCAT 
     1921 CTGATAATGA CAGAGTTATA GATCCAGGTA AAGTGGAAAC GTTAACAATC CGGAGGCTGC 
     1981 ATATTCCAAG GTTTTACTTA TTTTATGATC TGAGCACTTT ATATTCACTT ACAGAAAGAG 
     2041 TTAAAAGAAT CACAGTAGAA AACAGTAAAG TTTTTCTGGT TCCTTGTTTA CTTTCACAAC 
     2101 ATTTAAAATC ATTAGAATAC TTGGATCTCA GTGAAAATTT GATGGTTGAA GAATACTTGA 
     2161 AAAATTCAGC CTGTGAGGAT GCCTGGCCCT CTCTACAAAC TTTAATTTTA AGGCAAAATC 
     2221 ATTTGGCATC ATTGGAAAAA ACCGGAGAGA CTTTGCTCAC TCTGAAAAAC TTGACTAACA 
     2281 TTGATATCAG TAAGAATAGT TTTCATTCTA TGCCTGAAAC TTGTCAGTGG CCAGAAAAGA 
     2341 TGAAATATTT GAACTTATCC AGCACACGAA TACACAGTGT AACAGGCTGC ATTCCCAAGA 
     2401 CACTGGAAAT TTTAGATGTT AGCAACAACA ATCTCAATTT ATTTTCTTTG AATTTGCCGC 
     2461 AACTCAAAGA ACTTTATATT TCCAGAAATA AGTTGATGAC TCTACCAGAT GCCTCCCTCT 
     2521 TACCCATGTT ACTAGTATTG AAAATCAGTA GGAATGCAAT AACTACGTTT TCTAAGGAGC 
     2581 AACTTGACTC ATTTCACACA CTGAAGACTT TGGAAGCTGG TGGCAATAAC TTCATTTGCT 
     2641 CCTGTGAATT CCTCTCCTTC ACTCAGGAGC AGCAAGCACT GGCCAAAGTC TTGATTGATT 
     2701 GGCCAGCAAA TTACCTGTGT GACTCTCCAT CCCATGTGCG TGGCCAGCAG GTTCAGGATG 
     2761 TCCGCCTCTC GGTGTCGGAA TGTCACAGGA CAGCACTGGT GTCTGGCATG TGCTGTGCTC 
     2821 TGTTCCTGCT GATCCTGCTC ACGGGGGTCC TGTGCCACCG TTTCCATGGC CTGTGGTATA 
     2881 TGAAAATGAT GTGGGCCTGG CTCCAGGCCA AAAGGAAGCC CAGGAAAGCT CCCAGCAGGA 
     2941 ACATCTGCTA TGATGCATTT GTTTCTTACA GTGAGCGGGA TGCCTACTGG GTGGAGAACC 
     3001 TTATGGTCCA GGAGCTGGAG AACTTCAATC CCCCCTTCAA GTTGTGTCTT CATAAGCGGG 
     3061 ACTTCATTCC TGGCAAGTGG ATCATTGACA ATATCATTGA CTCCATTGAA AAGAGCCACA 
     3121 AAACTGTCTT TGTGCTTTCT GAAAACTTTG TGAAGAGTGA GTGGTGCAAG TATGAACTGG 
     3181 ACTTCTCCCA TTTCCGTCTT TTTGATGAGA ACAATGATGC TGCCATTCTC ATTCTTCTGG 
     3241 AGCCCATTGA GAAAAAAGCC ATTCCCCAGC GCTTCTGCAA GCTGCGGAAG ATAATGAACA 
     3301 CCAAGACCTA CCTGGAGTGG CCCATGGACG AGGCTCAGCG GGAAGGATTT TGGGTAAATC 
     3361 TGAGAGCTGC GATAAAGTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3421 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3481 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3541 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3601 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3661 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3721 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3781 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3841 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3901 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3961 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4021 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4081 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4141 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4201 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4261 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4321 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4381 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4441 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4501 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4561 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4621 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4681 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4741 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4801 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4861 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4921 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4981 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5041 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5101 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5161 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5221 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5281 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5341 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5401 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5461 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5521 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5581 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5641 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5701 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5761 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5821 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5881 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5941 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6001 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6061 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6121 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6181 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6241 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6301 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6361 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6421 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6481 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6541 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6601 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6661 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6721 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6781 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6841 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6901 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6961 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7021 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7081 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7141 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7201 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//