LOCUS       RC207419                7056 bp    DNA     circular  SYN 
DEFINITION  ALS2CR8 (Myc-DDK-tagged)-Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8 (ALS2CR8)
ACCESSION   NM_024744
VERSION     NM_024744.13
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3213..3304 
                     /label="Myc-DDK"  
     misc_feature    3334..3919 
                     /label="PolyA signal"  
     misc_feature    3391..3410 
                     /label="Restriction Enzyme"  
     misc_feature    4195..4867 
                     /label="ColE1"  
     misc_feature    5297..6091 
                     /label="Kan/Neo"  
     misc_feature    6126..6428 
                     /label="SV40 ori"  
     misc_feature    6514..6991 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3195..3201 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3195 
                     /label="CARF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACAATCT AATGATTCAT TAAGAGTCAA CCATAATGAC GGTGAAGAGT 
     1081 CAAAAACCAG TGCTCAAGTA TTTGAGCATC TAATCTGTAT GGACTCCAGG GATTCTTCCT 
     1141 TTGGACAAAA TGATTCTCCT ACAGTTTTGC CCATCACTAC TCGTGAAGCA AATAATTCAC 
     1201 TCATATCACA GAATATACCA GGGCCCCTGA CTCAGACACA GACTCTTTCT GCAGAGCAAT 
     1261 TCCATCTAGT GGACCAAAAT GGGCAGGCTA TTCAATATGA ACTTCAGTCA TTGGGGGAAT 
     1321 CCAATGCACA AATGATGATC GTTGCCAGCC CAACAGAAAA TGGACAGGTA CTTCGTGTAA 
     1381 TTCCACCTAC CCAGACAGGA ATGGCACAAG TGATTATACC TCAGGGGCAA CTTGTGGATG 
     1441 TGAATAGTCC TCGGGATGTC CCTGAAGAGA AACCCAGTAA CAGAAACTTA CCAACTGTAA 
     1501 GAGTGGATAC TCTAGCAGAC AATACCAGCA ATTACATTCT TCATCCTCAA ACATCCTTCC 
     1561 CATTGCCCAA AAAGTCAGTG ACCGGAATGC TGGAAGAACC CCTTCTGGGG CCTCTTCAGC 
     1621 CACTTTCTTC TAATACACCT ATATGGGCCT GCCGTCTTAG GAGCTGTGAG AAAATTGGAG 
     1681 ATTCATACCG TGGCTACTGT GTAAGTGAGA CTGAATTAGA AAGTGTCCTA ACATTTCACA 
     1741 AGCAGCAAAC ACAGAGTGTT TGGGGGACCC GTCAGTCTCC AAGCCCAGCC AAGCCTGCTA 
     1801 CACGCTTGAT GTGGAAATCC CAGTATGTTC CATATGATGG AATCCCATTT GTTAATGCAG 
     1861 GGAGTAGAGC TGTGGTAATG GAGTGTCAGT ATGGGCCAAG AAGAAAAGGT TTCCAGTTAA 
     1921 AAAAAGTCAG TGAGCAGGAA AGCAGGTCTT GTCAGCTCTA CAAAGCCACT TGTCCAGCTC 
     1981 GGATTTACAT TAAAAAGGTA CAGAAGTTTC CTGAATATAG AGTTCCTACA GACCCCAAAA 
     2041 TTGACAAGAA AATTATCAGA ATGGAGCAGG AGAAAGCTTT TAACATGCTA AAGAAGAACT 
     2101 TGGTAGATGC TGGTGGTGTT CTTAGGTGGT ATGTACAGTT ACCTACACAG CAAGCTCATC 
     2161 AGTATCATGA ATTAGAGACT CCCTGCCTCA CTTTGTCACC TTCTCCTTTT CCTGTGTCTT 
     2221 CTCTTGAAGA AGAGGAAACT GCAGTTAGAG ATGAGAATTG TGCATTACCC TCACGTTTAC 
     2281 ATCCTCAAGT AGCACATAAG ATTCAAGAAT TAGTATCACA GGGAATAGAA CAAGTGTATG 
     2341 CAGTAAGGAA ACAGCTAAGA AAATTTGTGG AAAGGGAACT GTTCAAACCC GATGAGGTAC 
     2401 CTGAAAGACA TAATTTATCT TTTTTTCCAA CTGTAAATGA TATAAAAAAT CACATCCATG 
     2461 AGGTACAGAA ATCCTTGAGA AATGGAGATA CGGTATATAA CTCAGAGATT ATTCCAGCAA 
     2521 CGCTTCAATG GACTACAGAC AGTGGGAATA TTCTCAAAGA GACCATGACA GTTACATTTG 
     2581 CAGAAGGAAA TTCACCAGGA GAATCAATTA CCACCAAAGT GGAAACAAAT CAGACCAGGG 
     2641 GTTCTTTGTC TCCTGAGCCA ACCCACTTGC TCTCCTCACT CTCCTCATTT CAGCCCAAAA 
     2701 TATTTACACA ACTACAGGGT TTGCAGTTAC AACCAAGGTA CACCTCTCCT GATGAATCAC 
     2761 CAGCTGTGGT ATCAGTAAAT AACCAGCCGT CCTCTAGTCC TTCAGGACTT CTGGATACAA 
     2821 TAGGAAGTGC TGTAATGAAT AATAATTCTC TACTGCTTGG TCAAAGTCAT AGCCTTCAAA 
     2881 GAGATACATG CTTAACCCAA AACAATAGTA CTGCCTCCAC CATGGGTAAC CTTCCAGAAC 
     2941 CAGATCAAAA TCTAGTTGCA ATGGACGAGC TGGTAGAAGT TGGAGATGTT GAGGATACAG 
     3001 GGAATCTGGA AGGAACTGTT CATCGGATTC TGTTGGGAGA TGTGCAGACT ATTCCAATAC 
     3061 AGATTATAGA CAACCACTCA GCTCTTATTG AAGAAAATCC AGAAAGTACC ATTTCTGTGA 
     3121 GCCAAGTTAA ACAAGAACCC AAAGAACCAG CATTGTCTAT GGAAGCAAAA AAAACTGTGG 
     3181 ACTATAAGAA ATTATCTGCT ACAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3241 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3301 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3361 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3421 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3481 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3541 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3601 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3661 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3721 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3781 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3841 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3901 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3961 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4021 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4081 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4141 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4201 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4261 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4321 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4381 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4441 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4501 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4561 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4621 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4681 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4741 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4801 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4861 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4921 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4981 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5041 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5101 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5161 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5221 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5281 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5341 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5401 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5461 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5521 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5581 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5641 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5701 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5761 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5821 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5881 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5941 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6001 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6061 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6121 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6181 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6241 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6301 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6361 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6421 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6481 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6541 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6601 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6661 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6721 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6781 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6841 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6901 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6961 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7021 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//