LOCUS       RC207414L1                8136 bp    DNA     circular  SYN 
DEFINITION  MAPK4 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 4 (MAPK4)
ACCESSION   NM_002747
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6427..6607 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7335..7994 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    6766..7334 
                     /label="ColE1"  
     misc_feature    5302..5322 
                     /label="LR50 primer"  
     misc_feature    5099..5170 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5311..5902 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5081..5087 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5081 
                     /label="MAPK4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCTGAGAAG GGTGACTGCA TCGCCAGTGT 
     3361 CTATGGGTAT GACCTCGGTG GGCGCTTTGT TGACTTCCAA CCCCTGGGCT TCGGTGTCAA 
     3421 TGGTTTGGTG CTGTCGGCCA TGGACAGCCG GGCCTGCCGG AAGGTCGCTG TGAAGAAGAT 
     3481 TGCCCTGAGC GATGCCCGCA GCATGAAGCA CGCGCTCCGA GAGATCAAGA TCATTCGGCG 
     3541 CCTGGACCAC GACAACATCG TCAAAGTGTA CGAGGTGCTC GGTCCCAAGG GCACTGACCT 
     3601 GCAGGGTGAG CTGTTCAAGT TCAGCGTGGC GTACATCGTC CAGGAGTACA TGGAGACCGA 
     3661 CCTGGCACGC CTGCTGGAGC AGGGCACGCT GGCAGAAGAG CATGCCAAGC TGTTCATGTA 
     3721 CCAGCTGCTC CGCGGGCTCA AGTACATCCA CTCCGCCAAC GTGCTGCACA GGGACCTGAA 
     3781 GCCCGCCAAC ATCTTCATCA GCACAGAGGA CCTCGTGCTC AAGATTGGGG ATTTCGGGTT 
     3841 GGCAAGGATC GTTGATCAGC ATTACTCCCA CAAGGGTTAT CTGTCAGAAG GGTTGGTAAC 
     3901 AAAGTGGTAC CGTTCCCCAC GACTGCTCCT TTCCCCCAAT AACTACACCA AAGCCATCGA 
     3961 CATGTGGGCC GCCGGCTGCA TCCTGTCTGA GATGCTTACG GGGAGAATGC TCTTTGCTGG 
     4021 GGCCCATGAG CTGGAGCAGA TGCAACTCAT CCTGGAGACC ATCCCTGTAA TCCGGGAGGA 
     4081 AGACAAGGAC GAGCTGCTCA GGGTGATGCC TTCCTTTGTC AGCAGCACCT GGGAGGTGAA 
     4141 GAGGCCTCTG CGCAAGCTGC TCCCTGAAGT GAACAGTGAA GCCATCGACT TTCTGGAGAA 
     4201 GATCCTGACC TTTAACCCCA TGGATCGCCT AACAGCTGAG ATGGGGCTGC AACACCCCTA 
     4261 CATGAGCCCA TACTCGTGCC CTGAGGACGA GCCCACCTCA CAACACCCCT TCCGCATTGA 
     4321 GGATGAGATC GACGACATCG TGCTGATGGC CGCTAACCAG AGCCAGCTGT CCAACTGGGA 
     4381 CACGTGCAGT TCCAGGTACC CTGTGAGCCT GTCGTCGGAC CTGGAGTGGC GGCCTGACCG 
     4441 GTGCCAGGAC GCCAGCGAGG TACAGCGCGA CCCGCGCGCG GGTTCGGCGC CACTGGCTGA 
     4501 GGACGTGCAG GTGGACCCGC GCAAGGACTC GCACAGCAGC TCCGAGCGCT TCCTAGAGCA 
     4561 GTCGCACTCG TCCATGGAGC GCGCCTTCGA GGCCGACTAC GGGCGCTCCT GCGACTACAA 
     4621 GGTGGGGTCG CCGTCCTACC TGGACAAGCT GCTGTGGCGC GACAACAAGC CGCACCACTA 
     4681 CTCGGAGCCC AAGCTCATCC TGGACCTGTC GCACTGGAAG CAGGCGGCCG GCGCGCCCCC 
     4741 CACGGCCACG GGGCTGGCGG ACACGGGGGC GCGCGAGGAC GAGCCGGCCA GCCTCTTCCT 
     4801 GGAGATCGCG CAGTGGGTCA AGAGCACGCA GGGCGGCCCA GAGCACGCCA GCCCGCCCGC 
     4861 CGACGACCCC GAGCGCCGCT TGTCTGCCTC GCCCCCCGGC CGCCCGGCCC CGGTGGACGG 
     4921 CGGCGCCAGC CCCCAGTTCG ACCTGGACGT GTTCATCTCC CGCGCCCTGA AGCTCTGCAC 
     4981 CAAGCCCGAG GACCTGCCGG ACAATAAACT GGGCGACCTC AATGGTGCGT GCATCCCCGA 
     5041 GCACCCTGGC GACCTCGTGC AGACCGAGGC CTTCTCCAAA GAAAGGTGGA CGCGTACGCG 
     5101 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     5161 CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC 
     5221 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC 
     5281 CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA 
     5341 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 
     5401 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 
     5461 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 
     5521 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 
     5581 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 
     5641 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 
     5701 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 
     5761 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 
     5821 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 
     5881 CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA 
     5941 AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT 
     6001 AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC 
     6061 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA 
     6121 AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 
     6181 AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC 
     6241 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA 
     6301 AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 
     6361 AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT 
     6421 GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG 
     6481 CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT 
     6541 GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT 
     6601 GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6661 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6721 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6781 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6841 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6901 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6961 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     7021 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     7081 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     7141 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     7201 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     7261 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     7321 ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 
     7381 AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC 
     7441 GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG 
     7501 GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA 
     7561 TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA 
     7621 CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT 
     7681 TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA 
     7741 ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA 
     7801 TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT 
     7861 ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA 
     7921 ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA 
     7981 TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8041 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8101 GTCCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//