LOCUS       RC207221                8226 bp    DNA     circular  SYN 
DEFINITION  IPO5 (Myc-DDK-tagged)-Human importin 5 (IPO5)
ACCESSION   NM_002271
VERSION     NM_002271.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4383..4474 
                     /label="Myc-DDK"  
     misc_feature    4504..5089 
                     /label="PolyA signal"  
     misc_feature    4561..4580 
                     /label="Restriction Enzyme"  
     misc_feature    5365..6037 
                     /label="ColE1"  
     misc_feature    6467..7261 
                     /label="Kan/Neo"  
     misc_feature    7296..7598 
                     /label="SV40 ori"  
     misc_feature    7684..8161 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4365..4371 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4365 
                     /label="IPO5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGAGGAT CAAGTTGGAA AACTAGAAGC AACAGAAAAC ACAATAAGCG 
     1081 CAATGGCGGC GGCCGCGGCG GAGCAGCAAC AGTTCTACCT GCTCCTGGGA AACCTGCTCA 
     1141 GCCCCGACAA TGTGGTCCGG AAACAGGCAG AGGAAACCTA TGAGAATATC CCAGGCCAGT 
     1201 CAAAGATCAC ATTCCTCTTA CAAGCCATCA GAAATACAAC AGCTGCTGAA GAGGCTAGAC 
     1261 AAATGGCCGC CGTTCTCCTA AGACGTCTCT TGTCCTCTGC ATTTGATGAA GTCTATCCAG 
     1321 CACTTCCCTC TGATGTTCAG ACTGCCATCA AGAGTGAGCT ACTCATGATT ATTCAGATGG 
     1381 AAACACAATC TAGCATGAGG AAAAAAGTTT GTGATATTGC GGCAGAACTG GCCAGGAATT 
     1441 TAATAGATGA GGATGGCAAT AACCAGTGGC CCGAAGGTTT GAAGTTCCTT TTTGATTCAG 
     1501 TCAGCTCTCA AAATGTGGGA CTGCGGGAAG CTGCCCTTCA CATTTTCTGG AACTTTCCTG 
     1561 GAATTTTTGG GAACCAGCAA CAACACTATT TAGATGTCAT CAAACGAATG TTAGTTCAGT 
     1621 GTATGCAAGA TCAGGAACAC CCGTCGATCA GGACGTTATC TGCTAGAGCT ACAGCTGCAT 
     1681 TTATACTTGC AAATGAGCAT AATGTTGCTC TGTTCAAACA TTTTGCAGAC TTGCTACCGG 
     1741 GATTCCTACA GGCGGTAAAT GACTCGTGCT ACCAGAATGA TGATTCTGTC CTAAAATCCC 
     1801 TCGTTGAGAT TGCAGATACT GTTCCAAAGT ATTTGCGTCC TCACTTGGAA GCAACTCTAC 
     1861 AGCTAAGTCT AAAGTTGTGT GGAGACACTA GCCTCAACAA TATGCAACGC CAGCTTGCCC 
     1921 TTGAAGTGAT CGTCACCCTC TCTGAGACTG CAGCTGCTAT GTTAAGAAAA CATACCAATA 
     1981 TTGTTGCACA GACTATTCCT CAGATGTTAG CAATGATGGT TGATTTGGAA GAAGATGAGG 
     2041 ACTGGGCAAA TGCAGATGAA CTAGAAGATG ATGATTTTGA CAGCAATGCA GTTGCAGGCG 
     2101 AGAGTGCTCT AGATCGAATG GCTTGCGGAC TTGGTGGAAA GCTCGTTCTG CCGATGATCA 
     2161 AGGAACACAT TATGCAAATG CTTCAAAATC CTGACTGGAA ATACCGGCAT GCAGGATTGA 
     2221 TGGCCTTATC TGCCATTGGT GAAGGGTGCC ACCAGCAAAT GGAAGGAATT CTAAATGAGA 
     2281 TCGTAAATTT TGTTTTACTT TTTCTCCAGG ATCCTCATCC AAGAGTAAGG TATGCAGCCT 
     2341 GTAATGCCGT GGGACAGATG GCTACAGATT TTGCACCTGG TTTCCAAAAG AAATTTCATG 
     2401 AGAAGGTGAT TGCAGCTCTG CTGCAGACCA TGGAAGACCA AGGCAATCAA CGTGTGCAGG 
     2461 CCCATGCAGC TGCTGCCCTC ATTAACTTTA CTGAAGACTG TCCCAAGTCA CTACTTATTC 
     2521 CATACTTGGA TAATTTGGTG AAACATCTGC ATTCCATTAT GGTACTGAAG CTTCAAGAGC 
     2581 TGATTCAGAA AGGCACCAAG TTAGTTTTGG AACAAGTTGT GACATCCATT GCATCAGTTG 
     2641 CCGATACTGC AGAAGAAAAA TTTGTCCCCT ACTATGATTT ATTTATGCCA TCACGGAAGC 
     2701 ACATCGTTGA GAATGCGGTT CAAAAAGAAC TGAGACTTCT GAGAGGAAAA ACTATTGAAT 
     2761 GCATTAGCCT CATTGGTCTG GCTGTTGGGA AGGAAAAATT CATGCAGGAT GCATCAGATG 
     2821 TGATGCAGCT TTTGTTAAAG ACCCAGACAG ACTTCAATGA TATGGAAGAT GATGATCCTC 
     2881 AGATCTCTTA CATGATCTCA GCATGGGCCA GAATGTGCAA AATCCTTGGA AAAGAATTTC 
     2941 AGCAATACCT TCCAGTGGTT ATGGGGCCTT TAATGAAGAC GGCTTCAATT AAGCCCGAAG 
     3001 TAGCCCTTTT AGATACCCAA GACATGGAGA ATATGAGTGA TGATGATGGT TGGGAATTTG 
     3061 TGAACCTTGG AGATCAGCAA AGCTTTGGTA TTAAAACTGC AGGACTAGAA GAAAAATCAA 
     3121 CTGCTTGCCA GATGTTGGTT TGCTATGCTA AGGAGTTAAA GGAAGGCTTT GTGGAGTACA 
     3181 CCGAACAGGT TGTCAAACTG ATGGTCCCTT TACTGAAATT TTATTTCCAC GATGGTGTTC 
     3241 GAGTGGCAGC AGCGGAATCC ATGCCTCTTC TCCTGGAGTG TGCAAGAGTC CGTGGTCCTG 
     3301 AGTACCTCAC ACAGATGTGG CATTTTATGT GTGATGCTCT AATTAAGGCC ATTGGTACAG 
     3361 AACCAGATTC AGACGTCCTC TCAGAAATAA TGCATTCTTT TGCAAAGTGC ATTGAAGTAA 
     3421 TGGGAGATGG ATGCCTTAAT AATGAACACT TTGAAGAACT GGGAGGTATA TTGAAAGCAA 
     3481 AGCTTGAAGA ACATTTTAAA AATCAAGAAT TACGACAAGT TAAAAGACAA GATGAAGACT 
     3541 ATGATGAACA GGTCGAAGAG TCACTACAAG ATGAGGATGA TAATGATGTT TATATTCTGA 
     3601 CCAAAGTGTC AGATATTTTA CACTCAATAT TCAGTAGCTA CAAAGAAAAG GTGTTACCAT 
     3661 GGTTTGAACA GCTGCTTCCA TTAATTGTCA ACCTCATTTG TCCACATAGA CCATGGCCAG 
     3721 ACAGACAATG GGGATTATGC ATCTTTGATG ATGTCATAGA ACACTGTAGT CCAGCCTCAT 
     3781 TTAAATACGC AGAATATTTC TTAAGACCAA TGCTCCAATA TGTATGTGAC AACAGCCCAG 
     3841 AAGTCAGGCA AGCAGCTGCA TATGGCCTGG GAGTCATGGC ACAGTACGGT GGAGATAATT 
     3901 ATCGCCCTTT TTGTACAGAA GCACTTCCCC TGCTGGTAAG AGTTATTCAG TCTGCGGATT 
     3961 CTAAGACCAA AGAAAATGTC AATGCTACAG AGAACTGCAT CTCAGCAGTA GGGAAAATCA 
     4021 TGAAGTTCAA GCCTGACTGT GTAAACGTTG AAGAGGTCCT TCCACACTGG TTGTCTTGGC 
     4081 TTCCACTACA TGAAGATAAA GAAGAAGCTG TTCAGACTTT CAATTATCTG TGTGACCTGA 
     4141 TTGAAAGTAA TCATCCAATT GTTCTTGGCC CAAACAATAC CAATCTGCCC AAAATATTTA 
     4201 GTATAATTGC GGAAGGAGAA ATGCACGAGG CAATTAAACA TGAAGATCCT TGTGCCAAAC 
     4261 GTCTGGCCAA TGTCGTTCGC CAAGTACAGA CTTCTGGAGG ACTGTGGACT GAGTGCATAG 
     4321 CACAGCTCAG TCCTGAGCAG CAGGCCGCCA TTCAGGAGCT CCTGAACTCT GCGACGCGTA 
     4381 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4441 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4501 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4561 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4621 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4681 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4741 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4801 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4861 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4921 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4981 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5041 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5101 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5161 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5221 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5281 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5341 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5401 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5461 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5521 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5581 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5641 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5701 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5761 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5821 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5881 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5941 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6001 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6061 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6121 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6181 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6241 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6301 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6361 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6421 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6481 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6541 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6601 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6661 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6721 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6781 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6841 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6901 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6961 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7021 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7081 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7141 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7201 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7261 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7321 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7381 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7441 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7561 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7621 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7681 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7741 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7801 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7861 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7921 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7981 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8041 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8101 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8161 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8221 AATTTT
//